Status
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Gene information
General information
TC031655
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001815,TC001816
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450299.1: 3410..11559
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031655
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031655 RA
      MEPSSSKVY KIKCCVPGC NDTTSKRHR FPKDVDLCN 
      SWVQNIGCP KLSTLSTAE LYNKFVCDI HFEEYYRVP 
      GTKRGLRKD AIPSLQLSR LGKEVEVSL SAVMVEPLP 
      GTSKDLDST QVVLQDVQS STSGTSPAT REEVFTPPN 
      LPTKSSDGR KRHGILQAV GARRAKDLT PRCKKFYAL 
      VGKWARQTA VKQHRIRNL TNRLREAEK VEKNYGQCE 
      ECVKMLTHS DGDIPMAVI EARQYRLST FTKPGSYLT 
      NLVSQYKAF ARSLKGAQK IKPKYGATT VEKLDNSSA 
      PPSSTPTYF EKNKGLVAR SHLIKTWTT TISSIQDKF 
      INKRNVFWA SSGVAASSV VVEPIPEPP PVPPEAAET 
      VSEIVSQVN ALGEPTFAS LGLGGKTPV GIAQTCFEY 
      LHINLGVPW WEAIVIGTL VIRICLFPL VIIAQRNAA 
      KMNNYMPQL QAIQLKMTE ARQSGNQLE VARYSQELM 
      IFMKEKQLN PLKNMIVPL AQMPVFVSF FMALREMSN 
      VPIESLRTG GLWWFTDLT VPDQYFLMP LITSATLFA 
      TIELGTDTA KLSSQNLQT MKYVLRAVP VIVLPFTIN 
      FPGAILCYW VSSNFISLL QVGFLKIPK VRNFFKIEP 
      MVTHKNLPM KPKGFTEGL KDSWTNLKI TRELEERQR 
      IDEMQFQKA GRGPIVKTY KYDPTKQTS SAISAKKR
    CDS sequence
    • >TC031655 RA
      atggagcct tcttcttca aaagtgtat aaaattaag 
      tgctgtgta ccaggttgt aatgataca accagtaag 
      cgacatcgt tttcctaaa gacgtcgat ttgtgcaat 
      tcttgggta caaaatatt ggttgtccg aaactatcc 
      actttatca acagcagag ctttacaac aagtttgtg 
      tgtgatata cattttgaa gagtattac cgggtacct 
      ggtacgaag agggggcta cggaaggac gccattcca 
      agcctgcaa ttatctagg ctgggcaag gaggtggag 
      gtgagcttg tcagctgtt atggtagag cctttgcct 
      gggacttca aaagatcta gattctaca caagttgtt 
      ctgcaagat gtccaatcg tccacgtca ggtacatct 
      ccagccaca agagaagaa gtttttact cctcctaat 
      cttccaacg aaatcttca gatggtaga aagcggcat 
      gggatatta caagctgta ggtgcaagg cgagccaag 
      gatttaact ccaaggtgc aaaaagttt tatgcgctc 
      gttggcaag tgggctcgg caaactgct gtaaaacaa 
      cacaggata cgcaactta acgaaccga ttgcgagaa 
      gcggaaaaa gttgaaaaa aactatggc caatgtgaa 
      gaatgtgtc aagatgctg acacattct gatggtgac 
      attccgatg gcagtaatt gaagccaga cagtaccgc 
      ctgtcaact ttcacaaag cctggaagc tatttaaca 
      aacttagtc tcgcaatat aaagccttc gctaggtcc 
      ctcaagggg gcacagaaa attaaaccc aagtatggg 
      gccacaaca gttgaaaaa ctcgacaat tctagtgcg 
      cccccttca tcaaccccc acctacttc gaaaaaaac 
      aaagggctt gtagctagg tcccacttg attaaaaca 
      tggaccacg acaatttcc tcaattcag gacaagttt 
      attaacaag aggaacgta ttttgggct tccagtggt 
      gtggcagcg tcgtcagtg gtcgtagag ccgatccct 
      gagccccca ccggtgccc cccgaagcc gcagagaca 
      gtttcggaa atagtcagt caagtgaat gcgttaggt 
      gagcccact tttgcctcc ttggggctg gggggcaag 
      acaccggtc gggatcgca cagacgtgc ttcgagtac 
      ctccatata aatctgggg gtgccgtgg tgggaggca 
      atagtcata ggaacgcta gttattcgc atttgtttg 
      tttcctttg gttataata gcgcagaga aatgcggct 
      aaaatgaac aattacatg ccacagctg caggcaatt 
      cagctgaag atgaccgaa gctagacag tcagggaat 
      cagttggag gtggcgaga tattcgcaa gagttgatg 
      atttttatg aaggagaaa cagttgaat ccgcttaaa 
      aatatgata gtgcctcta gcacagatg cccgttttc 
      gtgtcattt ttcatggct ttgagggag atgtccaat 
      gtcccgatt gagagcttg aggacaggg gggctgtgg 
      tggtttact gatttgaca gttcctgat cagtatttt 
      ttgatgcct ctgatcaca agtgccaca ctttttgcc 
      acaattgag ttgggtaca gatacagcg aaactgtca 
      tcacagaat ttgcaaacg atgaaatac gtgttgagg 
      gccgttcct gtaattgtc cttcccttc acaattaat 
      tttccgggg gctattttg tgctattgg gtgtcttcg 
      aattttatt tcgttgctc caagttgga tttttgaaa 
      attccaaaa gtgaggaat tttttcaaa attgagcca 
      atggtcacg cataagaat ttgccaatg aagcccaaa 
      gggttcacg gagggcttg aaggattcg tggactaac 
      ttaaaaatc acacgcgaa ttggaggaa cgtcaaaga 
      atagacgaa atgcaattt caaaaagct ggacgtggc 
      cccattgtc aagacttac aaatacgac ccaacaaaa 
      cagacttca tcggcgatt agtgccaaa aaacggtaa
    mRNA sequence
    • >TC031655 RA
      taattgatg gagccttct tcttcaaaa gtgtataaa 
      attaagtgc tgtgtacca ggttgtaat gatacaacc 
      agtaagcga catcgtttt cctaaagac gtcgatttg 
      tgcaattct tgggtacaa aatattggt tgtccgaaa 
      ctatccact ttatcaaca gcagagctt tacaacaag 
      tttgtgtgt gatatacat tttgaagag tattaccgg 
      gtacctggt acgaagagg gggctacgg aaggacgcc 
      attccaagc ctgcaatta tctaggctg ggcaaggag 
      gtggaggtg agcttgtca gctgttatg gtagagcct 
      ttgcctggg acttcaaaa gatctagat tctacacaa 
      gttgttctg caagatgtc caatcgtcc acgtcaggt 
      acatctcca gccacaaga gaagaagtt tttactcct 
      cctaatctt ccaacgaaa tcttcagat ggtagaaag 
      cggcatggg atattacaa gctgtaggt gcaaggcga 
      gccaaggat ttaactcca aggtgcaaa aagttttat 
      gcgctcgtt ggcaagtgg gctcggcaa actgctgta 
      aaacaacac aggatacgc aacttaacg aaccgattg 
      cgagaagcg gaaaaagtt gaaaaaaac tatggccaa 
      tgtgaagaa tgtgtcaag atgctgaca cattctgat 
      ggtgacatt ccgatggca gtaattgaa gccagacag 
      taccgcctg tcaactttc acaaagcct ggaagctat 
      ttaacaaac ttagtctcg caatataaa gccttcgct 
      aggtccctc aagggggca cagaaaatt aaacccaag 
      tatggggcc acaacagtt gaaaaactc gacaattct 
      agtgcgccc ccttcatca acccccacc tacttcgaa 
      aaaaacaaa gggcttgta gctaggtcc cacttgatt 
      aaaacatgg accacgaca atttcctca attcaggac 
      aagtttatt aacaagagg aacgtattt tgggcttcc 
      agtggtgtg gcagcgtcg tcagtggtc gtagagccg 
      atccctgag cccccaccg gtgcccccc gaagccgca 
      gagacagtt tcggaaata gtcagtcaa gtgaatgcg 
      ttaggtgag cccactttt gcctccttg gggctgggg 
      ggcaagaca ccggtcggg atcgcacag acgtgcttc 
      gagtacctc catataaat ctgggggtg ccgtggtgg 
      gaggcaata gtcatagga acgctagtt attcgcatt 
      tgtttgttt cctttggtt ataatagcg cagagaaat 
      gcggctaaa atgaacaat tacatgcca cagctgcag 
      gcaattcag ctgaagatg accgaagct agacagtca 
      gggaatcag ttggaggtg gcgagatat tcgcaagag 
      ttgatgatt tttatgaag gagaaacag ttgaatccg 
      cttaaaaat atgatagtg cctctagca cagatgccc 
      gttttcgtg tcatttttc atggctttg agggagatg 
      tccaatgtc ccgattgag agcttgagg acagggggg 
      ctgtggtgg tttactgat ttgacagtt cctgatcag 
      tattttttg atgcctctg atcacaagt gccacactt 
      tttgccaca attgagttg ggtacagat acagcgaaa 
      ctgtcatca cagaatttg caaacgatg aaatacgtg 
      ttgagggcc gttcctgta attgtcctt cccttcaca 
      attaatttt ccgggggct attttgtgc tattgggtg 
      tcttcgaat tttatttcg ttgctccaa gttggattt 
      ttgaaaatt ccaaaagtg aggaatttt ttcaaaatt 
      gagccaatg gtcacgcat aagaatttg ccaatgaag 
      cccaaaggg ttcacggag ggcttgaag gattcgtgg 
      actaactta aaaatcaca cgcgaattg gaggaacgt 
      caaagaata gacgaaatg caatttcaa aaagctgga 
      cgtggcccc attgtcaag acttacaaa tacgaccca 
      acaaaacag acttcatcg gcgattagt gccaaaaaa 
      cggtaaaat ttgttacgg atttaggta aacaaataa 
      ataatttag ttttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY