| General information | |||
| TC031655
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450299.1: 3410..11559 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031655 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07724 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031655 RA
MEPSSSKVY KIKCCVPGC NDTTSKRHR FPKDVDLCN SWVQNIGCP KLSTLSTAE LYNKFVCDI HFEEYYRVP GTKRGLRKD AIPSLQLSR LGKEVEVSL SAVMVEPLP GTSKDLDST QVVLQDVQS STSGTSPAT REEVFTPPN LPTKSSDGR KRHGILQAV GARRAKDLT PRCKKFYAL VGKWARQTA VKQHRIRNL TNRLREAEK VEKNYGQCE ECVKMLTHS DGDIPMAVI EARQYRLST FTKPGSYLT NLVSQYKAF ARSLKGAQK IKPKYGATT VEKLDNSSA PPSSTPTYF EKNKGLVAR SHLIKTWTT TISSIQDKF INKRNVFWA SSGVAASSV VVEPIPEPP PVPPEAAET VSEIVSQVN ALGEPTFAS LGLGGKTPV GIAQTCFEY LHINLGVPW WEAIVIGTL VIRICLFPL VIIAQRNAA KMNNYMPQL QAIQLKMTE ARQSGNQLE VARYSQELM IFMKEKQLN PLKNMIVPL AQMPVFVSF FMALREMSN VPIESLRTG GLWWFTDLT VPDQYFLMP LITSATLFA TIELGTDTA KLSSQNLQT MKYVLRAVP VIVLPFTIN FPGAILCYW VSSNFISLL QVGFLKIPK VRNFFKIEP MVTHKNLPM KPKGFTEGL KDSWTNLKI TRELEERQR IDEMQFQKA GRGPIVKTY KYDPTKQTS SAISAKKR
- >TC031655 RA
atggagcct tcttcttca aaagtgtat aaaattaag tgctgtgta ccaggttgt aatgataca accagtaag cgacatcgt tttcctaaa gacgtcgat ttgtgcaat tcttgggta caaaatatt ggttgtccg aaactatcc actttatca acagcagag ctttacaac aagtttgtg tgtgatata cattttgaa gagtattac cgggtacct ggtacgaag agggggcta cggaaggac gccattcca agcctgcaa ttatctagg ctgggcaag gaggtggag gtgagcttg tcagctgtt atggtagag cctttgcct gggacttca aaagatcta gattctaca caagttgtt ctgcaagat gtccaatcg tccacgtca ggtacatct ccagccaca agagaagaa gtttttact cctcctaat cttccaacg aaatcttca gatggtaga aagcggcat gggatatta caagctgta ggtgcaagg cgagccaag gatttaact ccaaggtgc aaaaagttt tatgcgctc gttggcaag tgggctcgg caaactgct gtaaaacaa cacaggata cgcaactta acgaaccga ttgcgagaa gcggaaaaa gttgaaaaa aactatggc caatgtgaa gaatgtgtc aagatgctg acacattct gatggtgac attccgatg gcagtaatt gaagccaga cagtaccgc ctgtcaact ttcacaaag cctggaagc tatttaaca aacttagtc tcgcaatat aaagccttc gctaggtcc ctcaagggg gcacagaaa attaaaccc aagtatggg gccacaaca gttgaaaaa ctcgacaat tctagtgcg cccccttca tcaaccccc acctacttc gaaaaaaac aaagggctt gtagctagg tcccacttg attaaaaca tggaccacg acaatttcc tcaattcag gacaagttt attaacaag aggaacgta ttttgggct tccagtggt gtggcagcg tcgtcagtg gtcgtagag ccgatccct gagccccca ccggtgccc cccgaagcc gcagagaca gtttcggaa atagtcagt caagtgaat gcgttaggt gagcccact tttgcctcc ttggggctg gggggcaag acaccggtc gggatcgca cagacgtgc ttcgagtac ctccatata aatctgggg gtgccgtgg tgggaggca atagtcata ggaacgcta gttattcgc atttgtttg tttcctttg gttataata gcgcagaga aatgcggct aaaatgaac aattacatg ccacagctg caggcaatt cagctgaag atgaccgaa gctagacag tcagggaat cagttggag gtggcgaga tattcgcaa gagttgatg atttttatg aaggagaaa cagttgaat ccgcttaaa aatatgata gtgcctcta gcacagatg cccgttttc gtgtcattt ttcatggct ttgagggag atgtccaat gtcccgatt gagagcttg aggacaggg gggctgtgg tggtttact gatttgaca gttcctgat cagtatttt ttgatgcct ctgatcaca agtgccaca ctttttgcc acaattgag ttgggtaca gatacagcg aaactgtca tcacagaat ttgcaaacg atgaaatac gtgttgagg gccgttcct gtaattgtc cttcccttc acaattaat tttccgggg gctattttg tgctattgg gtgtcttcg aattttatt tcgttgctc caagttgga tttttgaaa attccaaaa gtgaggaat tttttcaaa attgagcca atggtcacg cataagaat ttgccaatg aagcccaaa gggttcacg gagggcttg aaggattcg tggactaac ttaaaaatc acacgcgaa ttggaggaa cgtcaaaga atagacgaa atgcaattt caaaaagct ggacgtggc cccattgtc aagacttac aaatacgac ccaacaaaa cagacttca tcggcgatt agtgccaaa aaacggtaa
- >TC031655 RA
taattgatg gagccttct tcttcaaaa gtgtataaa attaagtgc tgtgtacca ggttgtaat gatacaacc agtaagcga catcgtttt cctaaagac gtcgatttg tgcaattct tgggtacaa aatattggt tgtccgaaa ctatccact ttatcaaca gcagagctt tacaacaag tttgtgtgt gatatacat tttgaagag tattaccgg gtacctggt acgaagagg gggctacgg aaggacgcc attccaagc ctgcaatta tctaggctg ggcaaggag gtggaggtg agcttgtca gctgttatg gtagagcct ttgcctggg acttcaaaa gatctagat tctacacaa gttgttctg caagatgtc caatcgtcc acgtcaggt acatctcca gccacaaga gaagaagtt tttactcct cctaatctt ccaacgaaa tcttcagat ggtagaaag cggcatggg atattacaa gctgtaggt gcaaggcga gccaaggat ttaactcca aggtgcaaa aagttttat gcgctcgtt ggcaagtgg gctcggcaa actgctgta aaacaacac aggatacgc aacttaacg aaccgattg cgagaagcg gaaaaagtt gaaaaaaac tatggccaa tgtgaagaa tgtgtcaag atgctgaca cattctgat ggtgacatt ccgatggca gtaattgaa gccagacag taccgcctg tcaactttc acaaagcct ggaagctat ttaacaaac ttagtctcg caatataaa gccttcgct aggtccctc aagggggca cagaaaatt aaacccaag tatggggcc acaacagtt gaaaaactc gacaattct agtgcgccc ccttcatca acccccacc tacttcgaa aaaaacaaa gggcttgta gctaggtcc cacttgatt aaaacatgg accacgaca atttcctca attcaggac aagtttatt aacaagagg aacgtattt tgggcttcc agtggtgtg gcagcgtcg tcagtggtc gtagagccg atccctgag cccccaccg gtgcccccc gaagccgca gagacagtt tcggaaata gtcagtcaa gtgaatgcg ttaggtgag cccactttt gcctccttg gggctgggg ggcaagaca ccggtcggg atcgcacag acgtgcttc gagtacctc catataaat ctgggggtg ccgtggtgg gaggcaata gtcatagga acgctagtt attcgcatt tgtttgttt cctttggtt ataatagcg cagagaaat gcggctaaa atgaacaat tacatgcca cagctgcag gcaattcag ctgaagatg accgaagct agacagtca gggaatcag ttggaggtg gcgagatat tcgcaagag ttgatgatt tttatgaag gagaaacag ttgaatccg cttaaaaat atgatagtg cctctagca cagatgccc gttttcgtg tcatttttc atggctttg agggagatg tccaatgtc ccgattgag agcttgagg acagggggg ctgtggtgg tttactgat ttgacagtt cctgatcag tattttttg atgcctctg atcacaagt gccacactt tttgccaca attgagttg ggtacagat acagcgaaa ctgtcatca cagaatttg caaacgatg aaatacgtg ttgagggcc gttcctgta attgtcctt cccttcaca attaatttt ccgggggct attttgtgc tattgggtg tcttcgaat tttatttcg ttgctccaa gttggattt ttgaaaatt ccaaaagtg aggaatttt ttcaaaatt gagccaatg gtcacgcat aagaatttg ccaatgaag cccaaaggg ttcacggag ggcttgaag gattcgtgg actaactta aaaatcaca cgcgaattg gaggaacgt caaagaata gacgaaatg caatttcaa aaagctgga cgtggcccc attgtcaag acttacaaa tacgaccca acaaaacag acttcatcg gcgattagt gccaaaaaa cggtaaaat ttgttacgg atttaggta aacaaataa ataatttag ttttat
