| General information | |||
| TC001386 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450585.1: 459..2703 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001386 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07723 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001386 RA
MPDNPPDFN AAVQVERVT VKPPPFWKG DPKLWFIQL EAQFNLGKI TSDTTKYHY VASAIDTSV LQQIADFVT NPPVVNQYE GIKQRLIST FSDSQERQL RKLLSEMDL GDKKPSQLL NEMKRLGDS AVSDELLKT LWLQRLPTH VQSVLATSS DPIQNLAQM ADKIVEIQH MSSVCTVAT PTEDMSAAI LQLTEEVAA LKAAYNTEI KPRRSRSRP QTPDRSSRD SCWYHRTFG NKAKKCITP CNFNKSENS TPRHRRLTI TDPTTGISF LIDSGSDIS IIPKKARQS TVSDLVLYA ANGTAIQTY GYERLTITL GLRRRLIWN FIIVDTKRA IIGADLLHY FDLLIDIRR ARLIDSTTG ISSAGTCIS ASHAAVLSV SPNARYRDL IREFPALTT FHNVEPILP HATQHLIET TGPPVASKP RRLPLDKLQ IAKREFEHM MALGICRPS NSPWASPLH MVPKKDSNW RPVGDYRRL NAVTKEDRY PIPHLHDFA HLLAGNTIF STVDLVRAY HQIPVEASS IPKTAITTP FGLFEFTRM QFGLRNAAQ SFQRFIHEV LNGLHFCFP YLNDILIAS TSERERTDH LRKVFERLL KYGLTINPE KCSFGNSKV KFLGYEVSA DGTKPLTDR VKVI
- >TC001386 RA
atgcccgat aacccacca gatttcaac gccgctgtc caggtcgag agggttact gttaaaccg ccacccttt tggaaaggt gatcccaag ctgtggttt atccaactt gaagcccaa ttcaacctc ggcaagata acgagcgat acaactaag tatcactac gtggccagt gctatcgac acatcagtt cttcagcaa attgccgat ttcgtgacc aatcctccc gttgttaac caatatgaa ggtatcaaa caacggctt atttctaca ttttcggat tctcaggaa cgccaattg cgtaaacta ctgtcggaa atggactta ggcgacaag aaaccttcg caattgctt aacgaaatg aagcgtctg ggtgattcg gctgtatcc gacgaatta ctgaaaact ttgtggtta caaagattg ccgacccac gtccaatcc gtgctcgca accagctcc gacccgatt cagaacctt gcgcaaatg gcagacaaa atcgtggag atacaacac atgtcctca gtttgtaca gttgcgacc ccaaccgaa gatatgtct gcagctatc ctgcaactc actgaggaa gtcgcagca ttaaaggct gcctacaat actgaaatt aagccgcga cgatcacga tcaagacca cagacgcca gaccgttca agcagggac tcttgttgg taccaccga acatttggc aataaagca aagaagtgt atcactccc tgcaacttc aacaagtcg gaaaactct acgccgcgc catcgacgc ctcaccata accgatcca accaccggt atttcgttt ttgattgac tctggttcg gatatttca atcatcccc aagaaagca cgacagtca acagtaagt gacctcgta ctttacgcc gccaatggc accgccatt caaacctac ggttacgag agacttacc atcactttg ggattacga cgtagatta atttggaac ttcatcatc gttgatacc aaacgagca ataattggc gccgattta ttgcattat ttcgacctt ttaatcgat attagacgg gccagacta attgatagc acgacagga atctccagt gcaggcaca tgtatttcc gcctctcac gctgcagtg ctgtcagtt tcaccgaac gctcggtat cgcgacctg atcagggaa tttccagct ctgacaact tttcacaat gtcgagcca atccttcca catgcgacc cagcatcta atcgaaacc accggacct ccagtggct tcaaaacca cggagactc cctctagac aaactacaa atagccaaa agagaattc gaacatatg atggcgcta gggatttgc agaccctcc aatagtccc tgggcaagt cccctccat atggtccct aaaaaggac agcaactgg cgtccggta ggtgactat agacgactc aacgcggtc actaaagag gatcgttac ccaattcct catttacat gacttcgct cacctgctt gctggtaat accattttt tcaaccgtt gacctcgtt agagcttac caccagatc ccggtcgaa gcgagctct attccgaaa accgccatt accacacct tttggcctt ttcgaattt acaagaatg caatttggt cttcgtaac gccgctcaa tcctttcaa agattcatc cacgaagtc ttgaatggt ctccacttt tgcttcccc tacctgaat gacattctc atcgcctcc actagcgaa agggaacgc actgaccat cttcgcaaa gtctttgaa agattatta aagtacggc cttactatt aatcccgaa aaatgttct tttggaaat tccaaagtg aagttcctg gggtacgaa gtctcagca gatggcacc aagccctta accgaccga gtcaaggtc atttaa
- >TC001386 RA
aacacgcgc atcgaaaac accacgcaa cagtgcacg ttacaacat gcccgataa cccaccaga tttcaacgc cgctgtcca ggtcgagag ggttactgt taaaccgcc acccttttg gaaaggtga tcccaagct gtggtttat ccaacttga agcccaatt caacctcgg caagataac gagcgatac aactaagta tcactacgt ggccagtgc tatcgacac atcagttct tcagcaaat tgccgattt cgtgaccaa tcctcccgt tgttaacca atatgaagg tatcaaaca acggcttat ttctacatt ttcggattc tcaggaacg ccaattgcg taaactact gtcggaaat ggacttagg cgacaagaa accttcgca attgcttaa cgaaatgaa gcgtctggg tgattcggc tgtatccga cgaattact gaaaacttt gtggttaca aagattgcc gacccacgt ccaatccgt gctcgcaac cagctccga cccgattca gaaccttgc gcaaatggc agacaaaat cgtggagat acaacacat gtcctcagt ttgtacagt tgcgacccc aaccgaaga tatgtctgc agctatcct gcaactcac tgaggaagt cgcagcatt aaaggctgc ctacaatac tgaaattaa gccgcgacg atcacgatc aagaccaca gacgccaga ccgttcaag cagggactc ttgttggta ccaccgaac atttggcaa taaagcaaa gaagtgtat cactccctg caacttcaa caagtcgga aaactctac gccgcgcca tcgacgcct caccataac cgatccaac caccggtat ttcgttttt gattgactc tggttcgga tatttcaat catccccaa gaaagcacg acagtcaac agtaagtga cctcgtact ttacgccgc caatggcac cgccattca aacctacgg ttacgagag acttaccat cactttggg attacgacg tagattaat ttggaactt catcatcgt tgataccaa acgagcaat aattggcgc cgatttatt gcattattt cgacctttt aatcgatat tagacgggc cagactaat tgatagcac gacaggaat ctccagtgc aggcacatg tatttccgc ctctcacgc tgcagtgct gtcagtttc accgaacgc tcggtatcg cgacctgat cagggaatt tccagctct gacaacttt tcacaatgt cgagccaat ccttccaca tgcgaccca gcatctaat cgaaaccac cggacctcc agtggcttc aaaaccacg gagactccc tctagacaa actacaaat agccaaaag agaattcga acatatgat ggcgctagg gatttgcag accctccaa tagtccctg ggcaagtcc cctccatat ggtccctaa aaaggacag caactggcg tccggtagg tgactatag acgactcaa cgcggtcac taaagagga tcgttaccc aattcctca tttacatga cttcgctca cctgcttgc tggtaatac cattttttc aaccgttga cctcgttag agcttacca ccagatccc ggtcgaagc gagctctat tccgaaaac cgccattac cacaccttt tggcctttt cgaatttac aagaatgca atttggtct tcgtaacgc cgctcaatc ctttcaaag attcatcca cgaagtctt gaatggtct ccacttttg cttccccta cctgaatga cattctcat cgcctccac tagcgaaag ggaacgcac tgaccatct tcgcaaagt ctttgaaag attattaaa gtacggcct tactattaa tcccgaaaa atgttcttt tggaaattc caaagtgaa gttcctggg gtacgaagt ctcagcaga tggcaccaa gcccttaac cgaccgagt caaggtcat ttaaactac ccgctacca aagacaccc aaagacgct agaaggttc ctaggtaca atttacttt taccgacgt tttgtacct catgcagcc gctcatcaa gccccgatc cacaatcta gtcaaaaac agcaaaaag aacgacaaa acaccgcta tcttggacc cccgaatcg aaagcagca ttcgagctg tgtaaaaca cgacctcgc tcaagcgat gctcttaat acaccctac agctactg
