Status
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Gene information
General information
TC001386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450585.1: 459..2703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001386
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07723

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001386 RA
        MPDNPPDFN AAVQVERVT VKPPPFWKG DPKLWFIQL 
        EAQFNLGKI TSDTTKYHY VASAIDTSV LQQIADFVT 
        NPPVVNQYE GIKQRLIST FSDSQERQL RKLLSEMDL 
        GDKKPSQLL NEMKRLGDS AVSDELLKT LWLQRLPTH 
        VQSVLATSS DPIQNLAQM ADKIVEIQH MSSVCTVAT 
        PTEDMSAAI LQLTEEVAA LKAAYNTEI KPRRSRSRP 
        QTPDRSSRD SCWYHRTFG NKAKKCITP CNFNKSENS 
        TPRHRRLTI TDPTTGISF LIDSGSDIS IIPKKARQS 
        TVSDLVLYA ANGTAIQTY GYERLTITL GLRRRLIWN 
        FIIVDTKRA IIGADLLHY FDLLIDIRR ARLIDSTTG 
        ISSAGTCIS ASHAAVLSV SPNARYRDL IREFPALTT 
        FHNVEPILP HATQHLIET TGPPVASKP RRLPLDKLQ 
        IAKREFEHM MALGICRPS NSPWASPLH MVPKKDSNW 
        RPVGDYRRL NAVTKEDRY PIPHLHDFA HLLAGNTIF 
        STVDLVRAY HQIPVEASS IPKTAITTP FGLFEFTRM 
        QFGLRNAAQ SFQRFIHEV LNGLHFCFP YLNDILIAS 
        TSERERTDH LRKVFERLL KYGLTINPE KCSFGNSKV 
        KFLGYEVSA DGTKPLTDR VKVI
      CDS sequence
      • >TC001386 RA
        atgcccgat aacccacca gatttcaac gccgctgtc 
        caggtcgag agggttact gttaaaccg ccacccttt 
        tggaaaggt gatcccaag ctgtggttt atccaactt 
        gaagcccaa ttcaacctc ggcaagata acgagcgat 
        acaactaag tatcactac gtggccagt gctatcgac 
        acatcagtt cttcagcaa attgccgat ttcgtgacc 
        aatcctccc gttgttaac caatatgaa ggtatcaaa 
        caacggctt atttctaca ttttcggat tctcaggaa 
        cgccaattg cgtaaacta ctgtcggaa atggactta 
        ggcgacaag aaaccttcg caattgctt aacgaaatg 
        aagcgtctg ggtgattcg gctgtatcc gacgaatta 
        ctgaaaact ttgtggtta caaagattg ccgacccac 
        gtccaatcc gtgctcgca accagctcc gacccgatt 
        cagaacctt gcgcaaatg gcagacaaa atcgtggag 
        atacaacac atgtcctca gtttgtaca gttgcgacc 
        ccaaccgaa gatatgtct gcagctatc ctgcaactc 
        actgaggaa gtcgcagca ttaaaggct gcctacaat 
        actgaaatt aagccgcga cgatcacga tcaagacca 
        cagacgcca gaccgttca agcagggac tcttgttgg 
        taccaccga acatttggc aataaagca aagaagtgt 
        atcactccc tgcaacttc aacaagtcg gaaaactct 
        acgccgcgc catcgacgc ctcaccata accgatcca 
        accaccggt atttcgttt ttgattgac tctggttcg 
        gatatttca atcatcccc aagaaagca cgacagtca 
        acagtaagt gacctcgta ctttacgcc gccaatggc 
        accgccatt caaacctac ggttacgag agacttacc 
        atcactttg ggattacga cgtagatta atttggaac 
        ttcatcatc gttgatacc aaacgagca ataattggc 
        gccgattta ttgcattat ttcgacctt ttaatcgat 
        attagacgg gccagacta attgatagc acgacagga 
        atctccagt gcaggcaca tgtatttcc gcctctcac 
        gctgcagtg ctgtcagtt tcaccgaac gctcggtat 
        cgcgacctg atcagggaa tttccagct ctgacaact 
        tttcacaat gtcgagcca atccttcca catgcgacc 
        cagcatcta atcgaaacc accggacct ccagtggct 
        tcaaaacca cggagactc cctctagac aaactacaa 
        atagccaaa agagaattc gaacatatg atggcgcta 
        gggatttgc agaccctcc aatagtccc tgggcaagt 
        cccctccat atggtccct aaaaaggac agcaactgg 
        cgtccggta ggtgactat agacgactc aacgcggtc 
        actaaagag gatcgttac ccaattcct catttacat 
        gacttcgct cacctgctt gctggtaat accattttt 
        tcaaccgtt gacctcgtt agagcttac caccagatc 
        ccggtcgaa gcgagctct attccgaaa accgccatt 
        accacacct tttggcctt ttcgaattt acaagaatg 
        caatttggt cttcgtaac gccgctcaa tcctttcaa 
        agattcatc cacgaagtc ttgaatggt ctccacttt 
        tgcttcccc tacctgaat gacattctc atcgcctcc 
        actagcgaa agggaacgc actgaccat cttcgcaaa 
        gtctttgaa agattatta aagtacggc cttactatt 
        aatcccgaa aaatgttct tttggaaat tccaaagtg 
        aagttcctg gggtacgaa gtctcagca gatggcacc 
        aagccctta accgaccga gtcaaggtc atttaa
      mRNA sequence
      • >TC001386 RA
        aacacgcgc atcgaaaac accacgcaa cagtgcacg 
        ttacaacat gcccgataa cccaccaga tttcaacgc 
        cgctgtcca ggtcgagag ggttactgt taaaccgcc 
        acccttttg gaaaggtga tcccaagct gtggtttat 
        ccaacttga agcccaatt caacctcgg caagataac 
        gagcgatac aactaagta tcactacgt ggccagtgc 
        tatcgacac atcagttct tcagcaaat tgccgattt 
        cgtgaccaa tcctcccgt tgttaacca atatgaagg 
        tatcaaaca acggcttat ttctacatt ttcggattc 
        tcaggaacg ccaattgcg taaactact gtcggaaat 
        ggacttagg cgacaagaa accttcgca attgcttaa 
        cgaaatgaa gcgtctggg tgattcggc tgtatccga 
        cgaattact gaaaacttt gtggttaca aagattgcc 
        gacccacgt ccaatccgt gctcgcaac cagctccga 
        cccgattca gaaccttgc gcaaatggc agacaaaat 
        cgtggagat acaacacat gtcctcagt ttgtacagt 
        tgcgacccc aaccgaaga tatgtctgc agctatcct 
        gcaactcac tgaggaagt cgcagcatt aaaggctgc 
        ctacaatac tgaaattaa gccgcgacg atcacgatc 
        aagaccaca gacgccaga ccgttcaag cagggactc 
        ttgttggta ccaccgaac atttggcaa taaagcaaa 
        gaagtgtat cactccctg caacttcaa caagtcgga 
        aaactctac gccgcgcca tcgacgcct caccataac 
        cgatccaac caccggtat ttcgttttt gattgactc 
        tggttcgga tatttcaat catccccaa gaaagcacg 
        acagtcaac agtaagtga cctcgtact ttacgccgc 
        caatggcac cgccattca aacctacgg ttacgagag 
        acttaccat cactttggg attacgacg tagattaat 
        ttggaactt catcatcgt tgataccaa acgagcaat 
        aattggcgc cgatttatt gcattattt cgacctttt 
        aatcgatat tagacgggc cagactaat tgatagcac 
        gacaggaat ctccagtgc aggcacatg tatttccgc 
        ctctcacgc tgcagtgct gtcagtttc accgaacgc 
        tcggtatcg cgacctgat cagggaatt tccagctct 
        gacaacttt tcacaatgt cgagccaat ccttccaca 
        tgcgaccca gcatctaat cgaaaccac cggacctcc 
        agtggcttc aaaaccacg gagactccc tctagacaa 
        actacaaat agccaaaag agaattcga acatatgat 
        ggcgctagg gatttgcag accctccaa tagtccctg 
        ggcaagtcc cctccatat ggtccctaa aaaggacag 
        caactggcg tccggtagg tgactatag acgactcaa 
        cgcggtcac taaagagga tcgttaccc aattcctca 
        tttacatga cttcgctca cctgcttgc tggtaatac 
        cattttttc aaccgttga cctcgttag agcttacca 
        ccagatccc ggtcgaagc gagctctat tccgaaaac 
        cgccattac cacaccttt tggcctttt cgaatttac 
        aagaatgca atttggtct tcgtaacgc cgctcaatc 
        ctttcaaag attcatcca cgaagtctt gaatggtct 
        ccacttttg cttccccta cctgaatga cattctcat 
        cgcctccac tagcgaaag ggaacgcac tgaccatct 
        tcgcaaagt ctttgaaag attattaaa gtacggcct 
        tactattaa tcccgaaaa atgttcttt tggaaattc 
        caaagtgaa gttcctggg gtacgaagt ctcagcaga 
        tggcaccaa gcccttaac cgaccgagt caaggtcat 
        ttaaactac ccgctacca aagacaccc aaagacgct 
        agaaggttc ctaggtaca atttacttt taccgacgt 
        tttgtacct catgcagcc gctcatcaa gccccgatc 
        cacaatcta gtcaaaaac agcaaaaag aacgacaaa 
        acaccgcta tcttggacc cccgaatcg aaagcagca 
        ttcgagctg tgtaaaaca cgacctcgc tcaagcgat 
        gctcttaat acaccctac agctactg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY