Status
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Gene information
General information
TC002071 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6092499..6101255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002071
    iBeetle screen iB_07720

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002071 RA
      MSITHPAEA LNLDFKQQR LFVDFFNNL PQKPSSTLR 
      FFSHGDFYT LHSSDASSA STFTGNIVK YMGEDPQLS 
      YVVLRKSQF EQYVRELLL VRQYRVEVY VKSTPNKIN 
      DWTLKYKGS PGNLSQFED VLFENASIT FSNDVLGLK 
      VTKGRIIAA ASVNSTELK FQVCEFSDN ECFTELEAL 
      IAQVGPREC VIPQGEAPD LIELKKVLE RNSVLVARA 
      KKSDFGAEN IVQDLNRLL YFAEGHQRS CLSFPETHN 
      SEALTCLNA VIKFLNLTG DEQNFNQFR ISSLDVHRY 
      VRLDNAALY SLNILPKPG VNTLESTNQ TSKNFSLKG 
      ILDHCVTPQ GRRLLETWI KQPLKDLNL IQERHEIVE 
      TFVKNPQLR QDLQTEVLA RLPDLLLLS KKLSSQKAT 
      LQDCYKVYQ VVAAVPLLL KNLKQVDNP SLQSALIHP 
      IEELRNDLD KYQDMIEEL LDLELVDRG EFLVKSSFS 
      PALEEISAR KLQIEERMQ KLLRAAAND LGFEEEKTI 
      KLDYTDQHR YFFRVTLKE EPVLRSNSR YQILDVVKG 
      GVRFTNSKL AELNDDYAE AKAEYVEQQ KTIISEMFA 
      VAAGYGDCL RNLNMFIAT VDVLVAFAN VAVWARVPY 
      IRPKMFEAG QSPLKLFKV RHPCIEQQE HVSFIPNSV 
      EFDSEHTLH IITGPNMCG KSTYIRSIG VCVLMAQIG 
      SFVPCNYAE IPIVDAILA RVGAEDCLL KGLSTFMVE 
      MIETATIIK SATPNSLVI IDELGRGTS TYDGCGLAF 
      AIAEFLAKE IKCFSLFAT HFHEITRLA EMHPSVCNK 
      HVTAVTTDN TITPLYQIR DGECDNSYG IHCARMVEF 
      SDDVIQSAV EHQKKLEHT AGMQFLRDF EPVLRRQVV 
      SEGDKIIQD TLEKVKGLD KLSDEDLVK EIAKLKEEL 
      EGTGNLFIK GLLQ
    CDS sequence
    • >TC002071 RA
      atgtcaata acacacccc gcggaagcg ctaaattta 
      gatttcaaa caacaacgc ctatttgtg gactttttc 
      aacaattta ccccagaaa ccatcctcc actttacgc 
      ttcttcagc catggcgat ttctacacc ctccacagc 
      tctgacgcc tcatccgcg tccactttt acgggaaac 
      atcgtcaaa tacatgggg gaagacccc cagctctcg 
      tacgttgtc ctccgaaag tcccaattt gagcaatac 
      gtccgggag ttgctcctc gtccgccaa taccgcgtg 
      gaagtctac gtcaaaagc acccccaac aagatcaac 
      gactggacg ttaaaatac aagggttcc cccgggaac 
      ctctcccag ttcgaggac gtcctcttc gagaacgcc 
      tccataacc ttcagcaat gacgtcctg ggcctcaaa 
      gtgaccaaa ggccgaata atcgcagcg gccagtgtg 
      aattcaacc gaattaaag ttccaagtg tgtgagttc 
      agtgataac gagtgcttc accgaactg gaggccctg 
      atcgcgcaa gtgggccct cgcgagtgt gtcattccg 
      caaggggaa gcccccgat ttaatcgag ttgaagaaa 
      gtgttagaa cgcaatagt gtgctagtg gctagggcg 
      aaaaaaagc gactttggg gccgaaaac attgtacag 
      gaccttaac aggctcctg tactttgcc gaaggccac 
      caaaggagt tgcctcagt ttccccgaa acccacaat 
      tcggaagct ctcacgtgt cttaacgct gtgatcaag 
      ttcctgaac ttgaccgga gacgagcaa aactttaac 
      cagttcagg attagttcg ctcgatgtc caccgttac 
      gtgcgtttg gacaacgcg gctctctat tcactcaac 
      atcctccct aaaccgggg gttaacaca cttgagtct 
      acaaaccaa acaagcaag aatttcagc ttgaaaggg 
      attctggac cattgtgtt acccctcaa ggtcgccga 
      ttgctcgaa acctggatc aagcaaccc ttgaaagac 
      ttgaacttg atccaggaa cggcacgaa atcgtcgaa 
      acctttgtc aaaaaccca cagctgaga caggacctc 
      caaaccgag gtcttggct cgcttaccc gacctcctc 
      ctcctctcc aaaaaactg tccagtcaa aaagccacg 
      ctgcaagat tgctataaa gtgtatcaa gtggtcgcg 
      gctgttccc ctcctcctc aaaaacctc aaacaggtt 
      gacaacccc tccctccaa tccgcccta atccacccg 
      attgaggag ttacgcaac gacctggac aagtatcag 
      gacatgatt gaggaactc ctggatttg gagctagtc 
      gatcggggg gagttcctg gtcaagagt agttttagc 
      cccgctttg gaggaaatc tcagccagg aagttgcaa 
      attgaggaa cgcatgcag aaattattg agggcggct 
      gccaatgac ttgggcttt gaggaggag aagacaatc 
      aagttggat tataccgac caacaccgg tattttttc 
      cgtgtgact cttaaggag gagccggtt ttgaggtcc 
      aattcgcgt tatcagatt ctggacgtt gtcaaaggc 
      ggtgttaga ttcactaac agcaaactg gccgagctt 
      aatgacgat tacgccgag gcgaaagcc gagtacgtc 
      gagcaacag aaaactatc atttcggag atgtttgct 
      gtggcagcc ggctatggc gattgtttg cgcaacttg 
      aacatgttc atagccacg gttgatgtt ttggtggcg 
      tttgccaac gtggcagtg tgggcacgt gtgccgtac 
      atacggccc aaaatgttc gaggctggt caaagccca 
      ctcaagttg ttcaaagtg aggcatccc tgtatcgag 
      caacaggaa cacgtgtcg ttcattccc aacagtgtt 
      gagtttgac tcagagcac actttgcac atcatcaca 
      gggcctaac atgtgcggg aaaagcacc tacattcgg 
      agtattggc gtttgtgtg ttaatggcc cagattggg 
      tcgttcgtg ccgtgcaat tacgccgaa ataccgata 
      gttgacgcg attctagca cgtgtgggt gctgaggat 
      tgtttattg aagggtctg agcacgttt atggtggaa 
      atgatcgaa acggccacg attatcaag agtgccacg 
      ccgaattca cttgttata attgacgag ttggggagg 
      gggacgtcc acttatgac gggtgtggc ttggcattc 
      gctatagct gagttttta gccaaagaa atcaagtgt 
      ttttcgttg tttgccaca cattttcac gagattacg 
      cgtctggcg gagatgcac cccagtgtg tgtaataag 
      cacgtgacc gcggtcacc actgataac accattacg 
      cccctttat cagatcagg gatggggag tgtgataac 
      agttatggg attcattgt gcgcgcatg gttgagttt 
      tccgatgat gtgattcag tcggccgtt gagcatcag 
      aagaagttg gaacatacg gccggaatg caatttctg 
      agggacttt gagccggtt ttaagacgc caagtcgtt 
      tcagaaggc gataaaata atccaagat actttagag 
      aaggtgaaa ggtttggat aagttatcc gatgaggat 
      ttggtcaag gaaatagcg aaactaaag gaagagctt 
      gaagggact ggaaatttg tttattaaa ggcttgctt 
      caataa
    mRNA sequence
    • >TC002071 RA
      atgtcaata acacacccc gcggaagcg ctaaattta 
      gatttcaaa caacaacgc ctatttgtg gactttttc 
      aacaattta ccccagaaa ccatcctcc actttacgc 
      ttcttcagc catggcgat ttctacacc ctccacagc 
      tctgacgcc tcatccgcg tccactttt acgggaaac 
      atcgtcaaa tacatgggg gaagacccc cagctctcg 
      tacgttgtc ctccgaaag tcccaattt gagcaatac 
      gtccgggag ttgctcctc gtccgccaa taccgcgtg 
      gaagtctac gtcaaaagc acccccaac aagatcaac 
      gactggacg ttaaaatac aagggttcc cccgggaac 
      ctctcccag ttcgaggac gtcctcttc gagaacgcc 
      tccataacc ttcagcaat gacgtcctg ggcctcaaa 
      gtgaccaaa ggccgaata atcgcagcg gccagtgtg 
      aattcaacc gaattaaag ttccaagtg tgtgagttc 
      agtgataac gagtgcttc accgaactg gaggccctg 
      atcgcgcaa gtgggccct cgcgagtgt gtcattccg 
      caaggggaa gcccccgat ttaatcgag ttgaagaaa 
      gtgttagaa cgcaatagt gtgctagtg gctagggcg 
      aaaaaaagc gactttggg gccgaaaac attgtacag 
      gaccttaac aggctcctg tactttgcc gaaggccac 
      caaaggagt tgcctcagt ttccccgaa acccacaat 
      tcggaagct ctcacgtgt cttaacgct gtgatcaag 
      ttcctgaac ttgaccgga gacgagcaa aactttaac 
      cagttcagg attagttcg ctcgatgtc caccgttac 
      gtgcgtttg gacaacgcg gctctctat tcactcaac 
      atcctccct aaaccgggg gttaacaca cttgagtct 
      acaaaccaa acaagcaag aatttcagc ttgaaaggg 
      attctggac cattgtgtt acccctcaa ggtcgccga 
      ttgctcgaa acctggatc aagcaaccc ttgaaagac 
      ttgaacttg atccaggaa cggcacgaa atcgtcgaa 
      acctttgtc aaaaaccca cagctgaga caggacctc 
      caaaccgag gtcttggct cgcttaccc gacctcctc 
      ctcctctcc aaaaaactg tccagtcaa aaagccacg 
      ctgcaagat tgctataaa gtgtatcaa gtggtcgcg 
      gctgttccc ctcctcctc aaaaacctc aaacaggtt 
      gacaacccc tccctccaa tccgcccta atccacccg 
      attgaggag ttacgcaac gacctggac aagtatcag 
      gacatgatt gaggaactc ctggatttg gagctagtc 
      gatcggggg gagttcctg gtcaagagt agttttagc 
      cccgctttg gaggaaatc tcagccagg aagttgcaa 
      attgaggaa cgcatgcag aaattattg agggcggct 
      gccaatgac ttgggcttt gaggaggag aagacaatc 
      aagttggat tataccgac caacaccgg tattttttc 
      cgtgtgact cttaaggag gagccggtt ttgaggtcc 
      aattcgcgt tatcagatt ctggacgtt gtcaaaggc 
      ggtgttaga ttcactaac agcaaactg gccgagctt 
      aatgacgat tacgccgag gcgaaagcc gagtacgtc 
      gagcaacag aaaactatc atttcggag atgtttgct 
      gtggcagcc ggctatggc gattgtttg cgcaacttg 
      aacatgttc atagccacg gttgatgtt ttggtggcg 
      tttgccaac gtggcagtg tgggcacgt gtgccgtac 
      atacggccc aaaatgttc gaggctggt caaagccca 
      ctcaagttg ttcaaagtg aggcatccc tgtatcgag 
      caacaggaa cacgtgtcg ttcattccc aacagtgtt 
      gagtttgac tcagagcac actttgcac atcatcaca 
      gggcctaac atgtgcggg aaaagcacc tacattcgg 
      agtattggc gtttgtgtg ttaatggcc cagattggg 
      tcgttcgtg ccgtgcaat tacgccgaa ataccgata 
      gttgacgcg attctagca cgtgtgggt gctgaggat 
      tgtttattg aagggtctg agcacgttt atggtggaa 
      atgatcgaa acggccacg attatcaag agtgccacg 
      ccgaattca cttgttata attgacgag ttggggagg 
      gggacgtcc acttatgac gggtgtggc ttggcattc 
      gctatagct gagttttta gccaaagaa atcaagtgt 
      ttttcgttg tttgccaca cattttcac gagattacg 
      cgtctggcg gagatgcac cccagtgtg tgtaataag 
      cacgtgacc gcggtcacc actgataac accattacg 
      cccctttat cagatcagg gatggggag tgtgataac 
      agttatggg attcattgt gcgcgcatg gttgagttt 
      tccgatgat gtgattcag tcggccgtt gagcatcag 
      aagaagttg gaacatacg gccggaatg caatttctg 
      agggacttt gagccggtt ttaagacgc caagtcgtt 
      tcagaaggc gataaaata atccaagat actttagag 
      aaggtgaaa ggtttggat aagttatcc gatgaggat 
      ttggtcaag gaaatagcg aaactaaag gaagagctt 
      gaagggact ggaaatttg tttattaaa ggcttgctt 
      caataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY