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Gene information
General information
TC011605 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15237554..15239231
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011605
    iBeetle screen iB_07718

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature potentially dorsalfluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011605 RA
      MVNKLNKSF YNTVLSQTF TPCGNYLVV GDIYGTISV 
      FHLSKLTNQ GSSITKEDQ LPKNAFTIK EDVQINSLL 
      TMKAFLVVG TVGEILGYS WKNVKTSKK PSCSWTIEI 
      PNSKDSFDK PDVNCLVFE PENNLLFAA CGDNNVYVF 
      NIETRKEVR CFKGHKNYV HCINFCNNK LVSGGEDGL 
      VLLWDVRGG PPVSKIEPH SRDKVARVE LGPWIGGVG 
      INDDWLLCG GGPRLSLWH LRSLNIATV FPIDDKGIH 
      VAEVYQDRI FAGGRSNLF YQMSFNGDI ITEIPTSPV 
      TTYSAVHQD EPFQTLCIA GSSPKIDVC SNFIYKNLQ 
      LSLY
    CDS sequence
    • >TC011605 RA
      atggtgaac aagttgaat aaatcgttc tataacact 
      gttttgagc cagactttc accccgtgt ggcaactat 
      ctcgtcgta ggtgacatt tacggcaca atttcggtc 
      ttccacctt tccaagcta acaaaccaa ggatcttcg 
      atcacaaaa gaagaccaa ctgcccaaa aacgcattc 
      acgattaaa gaagacgtc caaatcaac agtttattg 
      accatgaaa gcgttcctt gttgtgggg acagttggc 
      gagattttg gggtactcc tggaagaac gtcaaaacg 
      agcaaaaaa ccctcctgc tcgtggaca attgaaatc 
      ccgaactcc aaagacagt ttcgacaaa cctgacgtt 
      aattgttta gtgtttgag cccgaaaat aatttatta 
      tttgccgcc tgtggcgat aataatgtt tacgttttc 
      aacatcgag acacgaaaa gaagtgcgg tgttttaaa 
      gggcacaag aattacgtt cactgcatc aatttctgt 
      aacaataaa ctggtttcg gggggcgaa gacgggctt 
      gtgctgcta tgggatgtg agaggaggc cccccagtg 
      agcaaaatc gagccccac agtagggat aaagtggcc 
      agggtggag ctggggccc tggataggg ggcgttggg 
      attaacgat gattggctg ttgtgcggc ggcggcccc 
      cgactgtct ttatggcac ttgcgctcc ctgaatatc 
      gccacagtg ttccccatt gacgataaa gggattcat 
      gtggcggag gtctaccaa gacaggatt ttcgcaggg 
      gggcgctct aatttattc taccaaatg agttttaac 
      ggggacatt atcacggaa attccgacg tctcctgtg 
      accacgtac agtgcggtg catcaggat gagcccttc 
      cagactttg tgcattgcg gggtcgagc cccaaaatc 
      gacgtttgt agcaacttt atctacaaa aatttgcaa 
      ctatctctg tattaa
    mRNA sequence
    • >TC011605 RA
      tttacgttt gttttagag tttatagta taatatgtt 
      taattatga cataaaaaa aacataaat aaagttagg 
      tatgcaact aacttaacc gcgttgcat gttaagttg 
      cctgtatta agtcgattg aggttatat tgtttaatg 
      tttggggct gtatttatg ttaaataca cgtttgcgc 
      cctttgtaa tcgtataat tggtaataa aaccagact 
      ttgtacagt aatattgcc tattaaacg tgcgtttaa 
      ttcacaaaa agttatttt ttcttaacc tcaaaatgg 
      tgaacaagt tgaataaat cgttctata acactgttt 
      tgagccaga ctttcaccc cgtgtggca actatctcg 
      tcgtaggtg acatttacg gcacaattt cggtcttcc 
      acctttcca agctaacaa accaaggat cttcgatca 
      caaaagaag accaactgc ccaaaaacg cattcacga 
      ttaaagaag acgtccaaa tcaacagtt tattgacca 
      tgaaagcgt tccttgttg tggggacag ttggcgaga 
      ttttggggt actcctgga agaacgtca aaacgagca 
      aaaaaccct cctgctcgt ggacaattg aaatcccga 
      actccaaag acagtttcg acaaacctg acgttaatt 
      gtttagtgt ttgagcccg aaaataatt tattatttg 
      ccgcctgtg gcgataata atgtttacg ttttcaaca 
      tcgagacac gaaaagaag tgcggtgtt ttaaagggc 
      acaagaatt acgttcact gcatcaatt tctgtaaca 
      ataaactgg tttcggggg gcgaagacg ggcttgtgc 
      tgctatggg atgtgagag gaggccccc cagtgagca 
      aaatcgagc cccacagta gggataaag tggccaggg 
      tggagctgg ggccctgga tagggggcg ttgggatta 
      acgatgatt ggctgttgt gcggcggcg gcccccgac 
      tgtctttat ggcacttgc gctccctga atatcgcca 
      cagtgttcc ccattgacg ataaaggga ttcatgtgg 
      cggaggtct accaagaca ggattttcg caggggggc 
      gctctaatt tattctacc aaatgagtt ttaacgggg 
      acattatca cggaaattc cgacgtctc ctgtgacca 
      cgtacagtg cggtgcatc aggatgagc ccttccaga 
      ctttgtgca ttgcggggt cgagcccca aaatcgacg 
      tttgtagca actttatct acaaaaatt tgcaactat 
      ctctgtatt aagtcttta gtaaaataa taaaccata 
      aataattcg tccagaatt tgaatttga cagctgtca 
      ttgatgctc tttaggtta ggataaggt accgttaaa 
      gagtcaaaa atcgctcga atgtgaccc caaatcgtt 
      tataaccct tattatttt tttaagaac aattaagtt 
      acagtatgg ccaagtttg agtgctcaa attggcgta 
      ttattgtgt gaaatcgca aatttcata tttaagttg 
      gcaggactg tattaaaag acaattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY