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Gene information
General information
TC011650 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15998407..16000344
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011650
    iBeetle screen iB_07710

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011650 RA
      MPHEDLLIS ERCGSLGNP IKYTRQGQN GIIRNREIS 
      QESVADSVL QSVLGFFRE VLLPHGYPD SVSEDYFEY 
      QLWDTVQAF CSTITGIFT TQAILKGVG VGSAEASAL 
      SATITWIMK DGTGMVGRI IFAWWKGKS LDSNCKKWR 
      LFADLLNDG AMILELSIP HVTHLSTEI LCLSTLMKA 
      MVGVAGGAT RASITNHQA IKGNMAEIA AKDGSQETL 
      VNLIASFTG IFLINYFTD ATTQWIVTF LLIFLHLYS 
      NYQAVTALI FKNLNNLRM TLILKSYLA VNAVLGPEC 
      INKTESVFL GFGTSVEKF CGFKIVLGQ ALGKTLRRY 
      NSDELKSLT EIYKDKKYL IVPDLKTRT IYVTFEKGE 
      TPQEVIAGF YHAVLLGLA TCIYNRVTL EIYSKRQLH 
      HSTPITRLS TFMKNYQNN NIASNLPPP LLQAFTEFV 
      GQEIEMFFT ALQVNGWKT DSHCLYVDE WRAQWKDFD 
      KKSL
    CDS sequence
    • >TC011650 RA
      atgccccat gaagattta ttaatcagc gaacgatgt 
      ggctctttg ggcaacccc atcaaatac acccgtcaa 
      ggtcagaac ggtatcatc cgcaaccgc gaaataagc 
      caagaatcc gtcgcggat tccgtgctt caaagcgtt 
      ttgggcttc ttccgagaa gtccttttg ccccatggc 
      tatccagac agtgtaagc gaagattac tttgagtac 
      caactatgg gacacggtc caagcattt tgtagtact 
      atcactggg atttttacc acgcaggcg attttaaag 
      ggtgtgggg gtgggcagt gccgaggct tcggccctt 
      tctgccacc atcacctgg atcatgaaa gacggcact 
      ggaatggtc ggacgaatc atcttcgca tggtggaaa 
      ggaaaatcc ctagactcc aattgtaaa aagtggcga 
      cttttcgcc gacttatta aatgatgga gccatgatt 
      ctagaactg tcaatacca cacgtcacg cacctctcc 
      actgaaatt ctttgtctt tcaacacta atgaaagcg 
      atggtggga gttgcaggt ggcgccact cgtgcttcc 
      ataaccaat caccaagcg attaaaggc aacatggcg 
      gagattgcc gccaaagat ggcagccaa gagacactc 
      gtcaacttg attgcaagt ttcactgga atttttcta 
      attaattac tttacggat gccaccact caatggatt 
      gtgacattt ctcttgatt tttctccac ttgtattcg 
      aattatcaa gccgtcact gccctcatt ttcaagaat 
      ttgaataat ttacgaatg accttgata ttgaagagt 
      tatctggct gttaatgca gtgctaggc cctgaatgt 
      atcaataaa actgaatca gtttttttg ggatttggg 
      acaagcgtt gagaagttt tgtggtttt aagatagtt 
      ctgggacag gcacttgga aagactttg cgacgttat 
      aattcagat gaattgaaa agtttgacg gaaatttac 
      aaagacaaa aaatatctg attgtacct gatttaaaa 
      acgagaacc atttatgta acttttgaa aaaggtgaa 
      acgcctcaa gaagttata gcaggcttt tatcatgca 
      gtattactt ggacttgcc acttgtatt tataataga 
      gttacactt gaaatttat tcaaagcga caattacat 
      cattccact ccaatcacc agattgagt acttttatg 
      aaaaactac caaaataat aacatagct agtaatctc 
      cccccaccc ctcctacaa gcttttacc gaatttgtg 
      ggacaagaa atcgaaatg tttttcaca gcgctgcaa 
      gtcaatggg tggaaaacc gacagtcac tgcctttac 
      gtggacgag tggcgagcg caatggaaa gactttgat 
      aaaaagtca ttataa
    mRNA sequence
    • >TC011650 RA
      tcattcgag aatttaaat gaatcatcc tcagagata 
      agcgaaaac caaggaaac caaccaagc ccttttctt 
      gtagtattt caaatctgg cgcgtcctc gacctcgtc 
      cattgccat cgcctcgtt gtcaaaatc agctgtttg 
      tcaaaagtg tgtccacaa caaatttct attgatgtc 
      agataacgt ttgtggtca gccatcccg tcgtcatcg 
      tgtataatg ccccatgaa gatttatta atcagcgaa 
      cgatgtggc tctttgggc aaccccatc aaatacacc 
      cgtcaaggt cagaacggt atcatccgc aaccgcgaa 
      ataagccaa gaatccgtc gcggattcc gtgcttcaa 
      agcgttttg ggcttcttc cgagaagtc cttttgccc 
      catggctat ccagacagt gtaagcgaa gattacttt 
      gagtaccaa ctatgggac acggtccaa gcattttgt 
      agtactatc actgggatt tttaccacg caggcgatt 
      ttaaagggt gtgggggtg ggcagtgcc gaggcttcg 
      gccctttct gccaccatc acctggatc atgaaagac 
      ggcactgga atggtcgga cgaatcatc ttcgcatgg 
      tggaaagga aaatcccta gactccaat tgtaaaaag 
      tggcgactt ttcgccgac ttattaaat gatggagcc 
      atgattcta gaactgtca ataccacac gtcacgcac 
      ctctccact gaaattctt tgtctttca acactaatg 
      aaagcgatg gtgggagtt gcaggtggc gccactcgt 
      gcttccata accaatcac caagcgatt aaaggcaac 
      atggcggag attgccgcc aaagatggc agccaagag 
      acactcgtc aacttgatt gcaagtttc actggaatt 
      tttctaatt aattacttt acggatgcc accactcaa 
      tggattgtg acatttctc ttgattttt ctccacttg 
      tattcgaat tatcaagcc gtcactgcc ctcattttc 
      aagaatttg aataattta cgaatgacc ttgatattg 
      aagagttat ctggctgtt aatgcagtg ctaggccct 
      gaatgtatc aataaaact gaatcagtt tttttggga 
      tttgggaca agcgttgag aagttttgt ggttttaag 
      atagttctg ggacaggca cttggaaag actttgcga 
      cgttataat tcagatgaa ttgaaaagt ttgacggaa 
      atttacaaa gacaaaaaa tatctgatt gtacctgat 
      ttaaaaacg agaaccatt tatgtaact tttgaaaaa 
      ggtgaaacg cctcaagaa gttatagca ggcttttat 
      catgcagta ttacttgga cttgccact tgtatttat 
      aatagagtt acacttgaa atttattca aagcgacaa 
      ttacatcat tccactcca atcaccaga ttgagtact 
      tttatgaaa aactaccaa aataataac atagctagt 
      aatctcccc ccacccctc ctacaagct tttaccgaa 
      tttgtggga caagaaatc gaaatgttt ttcacagcg 
      ctgcaagtc aatgggtgg aaaaccgac agtcactgc 
      ctttacgtg gacgagtgg cgagcgcaa tggaaagac 
      tttgataaa aagtcatta taaatattt ttatacagt 
      ttttatggt tgaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY