Status
Please wait ...
Gene information
General information
TC011701 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 14858534..14862437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011701
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07700

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011701 RA
      MDFEFDEID SKALEDLME TVTKTSAKS GPSPEHLKV 
      LTKFFGHKT FRPKQWEII FAIMDKRDV CAIMSTGYG 
      KSLCFQYPS TFLKGVTLV VSPLIALMQ DQVLALTVS 
      NIPACLLGS AQNEQRKVI EDILQNKYS IVYITPEFC 
      SGDLGLELL KKMAQQLKI VLIAIDEAH CISSWGHDF 
      RSQYRNLGN LRQIFPRVP VIAVTATAT TRVRGDIVK 
      SLQLRDPLI VCSGFDRPN LYFEVYQRA GGGAFKDLT 
      KAMVYEKSE WKFSGPTII YCITRRQTE DLCEILKNC 
      GVRCRVYHA GLSQKERQE AHEEFVRDK VDVIVATIA 
      FGMGIDKPD IRNVIHYGS SNSVESYYQ EVGRAGRDG 
      LPARCVTIY SSGDFQILR NISGGSVKK ETALRQIED 
      YLTTRKCRR RFILEFFED EVGEDKPKQ RCCDVCTQK 
      LYDNRTDQE KYEGLDEKG RYDFTEDAK KFLKTVALF 
      GGKCGLTTY ILFLRGSQS TRLTEDKRK HPLNGCGKD 
      QSEEWWKCI AKILTAENC LSEKNQKLS SGFTYQALS 
      LTNKGQSMI NGSEKLLIP PPSTVLTLL KQKPAKESV 
      WLTTRPVNT PSTSKQPEN TETPEEIEQ RMSLYRKLL 
      DQRTDIAAK LDCMPYMVA SNTVLMEMS KCKPKTIAD 
      LRTLNLEGF PEAKILKFG AAFLGVIAR QLKLNKPKI 
      REILLQNPL PDVSMTVTA EVSCSMLES GLTVGEIAA 
      KRGVTVGTI ASHLISGIK FGFPVKMSE LGVSEEHRD 
      AIIKAYRGL VGPPLSPVL GFNSVRLSE MKERCPPDF 
      TFDQIKTVL AYLEVRCHL AALNFDYEE FEDFNFSDV 
      DLKTEMKSE EEGPPLKRE KKDCDPCVD AILDSPPRE 
      VKAEPKKSK KALPPWLVP KKLS
    CDS sequence
    • >TC011701 RA
      atggatttc gagttcgat gaaatcgat agtaaagca 
      ctggaagac ctcatggaa actgtaacc aagacctca 
      gcgaagtct ggaccaagt cctgaacac ctcaaagtc 
      ttaactaaa tttttcggc cacaaaacc ttccgacca 
      aagcaatgg gaaatcatc tttgcaatc atggacaag 
      agagacgtg tgtgcaatt atgagcact ggctatggc 
      aagtccttg tgcttccaa tacccttcc acttttctc 
      aaaggagtc actcttgtc gtctcaccc ttgattgca 
      ctgatgcaa gaccaagtc ctcgcacta actgtttcc 
      aatattccg gcgtgtctt ttggggtcg gcgcaaaac 
      gagcaaagg aaagtcatt gaggacatt ttgcagaac 
      aagtacagt attgtttac atcacccct gagttctgt 
      tcaggcgac ttggggctt gagcttttg aaaaaaatg 
      gcgcaacag ctcaaaatt gtcctgatt gcgatcgat 
      gaggcccat tgtattagc tcctggggg catgatttt 
      aggtcgcag tatcgcaat ttgggaaat ttgcgacag 
      atttttccg cgggttcct gtgatcgca gtcacggcg 
      actgcaaca accagagtg cgaggcgat attgtcaaa 
      agtctccaa ctgcgggac cctcttatt gtttgttcc 
      ggcttcgac cgcccgaat ctctacttc gaagtgtac 
      caaagagca ggcggtggc gccttcaaa gacctgacc 
      aaggccatg gtttacgaa aaatccgaa tggaaattc 
      tctggtcca acaattatt tattgtatc acccgtaga 
      cagactgaa gatttatgc gaaatttta aaaaattgt 
      ggtgttagg tgtcgagtg tatcacgcc ggtttgtcg 
      caaaaggaa cgtcaagag gctcatgag gagtttgtt 
      cgcgacaaa gtcgacgtg attgtggca acaattgcc 
      ttcggcatg ggcatagac aagcctgat attcgaaat 
      gtgatacat tacggctca tctaatagt gtcgaaagt 
      tactaccag gaagtaggc cgtgctgga cgcgacgga 
      cttccggcg cgctgtgtc acgatttac agttccggc 
      gattttcaa atcctccga aacatcagc ggaggctcg 
      gtcaaaaaa gaaacggct ttgaggcaa attgaggat 
      tatttgacc acgcgcaag tgtcggcgc cgcttcatt 
      ttggagttt tttgaggat gaagtgggc gaggataag 
      ccgaagcag aggtgttgt gacgtgtgt acacaaaaa 
      ctttatgat aatcgcacc gatcaggag aaatacgaa 
      ggtctggat gaaaaaggc cgttatgat tttacagaa 
      gatgcgaaa aagtttctc aaaactgtc gcacttttt 
      ggtggaaag tgcggtttg accacttat atccttttt 
      ctgagaggg tctcagtcg acaaggttg actgaagat 
      aaaaggaaa catccgctg aatggatgt gggaaggac 
      caatcggag gaatggtgg aagtgcata gcgaaaatc 
      ctcactgcg gagaattgt ctgagcgaa aaaaaccaa 
      aaattatca tcaggtttc acctatcaa gccttatca 
      ttaacaaac aaaggacaa tcgatgatt aacggaagt 
      gagaaatta ctcatcccg ccaccgtca actgtcctc 
      acgttattg aaacaaaag ccggccaaa gaatcggtt 
      tggttaacc actcgaccg gttaacacc ccctcaacg 
      agtaaacag ccggaaaat accgaaact ccggaggaa 
      attgagcaa cggatgtca ctctaccgg aaactactc 
      gaccaaagg accgatatt gcggcaaaa ttggactgt 
      atgccatac atggtggcc tcaaacaca gtcttaatg 
      gaaatgtca aaatgtaaa ccgaaaaca atcgccgat 
      cttcgaacc ttaaatctt gagggcttc cccgaggcc 
      aaaatactc aaatttggg gccgccttt ctgggggtc 
      attgcgaga caactcaag ttgaataaa ccaaaaatt 
      cgggagatt ctactccaa aacccttta cccgacgtt 
      agcatgact gtcactgcc gaagtctcg tgctccatg 
      ctagagtcg gggctcact gtgggcgaa atcgccgcc 
      aaaaggggg gtcaccgtg gggacaatt gcctcgcac 
      ctcatttcc ggcataaaa ttcgggttt ccggtgaaa 
      atgtcggaa ctgggggtc agtgaggag catcgcgac 
      gccataata aaagcctac agggggctt gtggggccc 
      cccttaagt ccagtttta gggtttaac agtgttagg 
      ttgtccgag atgaaggag aggtgcccc ccggatttt 
      accttcgac cagattaag acagttttg gcgtatttg 
      gaggtcagg tgtcacttg gcggctctc aattttgac 
      tacgaggag tttgaagac tttaacttt agtgatgtt 
      gatttgaag actgaaatg aagagtgag gaggagggg 
      ccccctttg aaacgcgag aagaaagac tgtgatccg 
      tgtgtggac gcaattttg gattcgccc cccagggaa 
      gtcaaagct gagccaaaa aaatcgaaa aaggcgctc 
      ccgccttgg ctcgtgccc aaaaaattg tcctaa
    mRNA sequence
    • >TC011701 RA
      tggaaagta tggaaacat aaccataga ataacataa 
      ccctgtatg tcaaatgtt tgaaatcag tttttttgt 
      tccgttttt tccttttgt ctaaaaaat ggatttcga 
      gttcgatga aatcgatag taaagcact ggaagacct 
      catggaaac tgtaaccaa gacctcagc gaagtctgg 
      accaagtcc tgaacacct caaagtctt aactaaatt 
      tttcggcca caaaacctt ccgaccaaa gcaatggga 
      aatcatctt tgcaatcat ggacaagag agacgtgtg 
      tgcaattat gagcactgg ctatggcaa gtccttgtg 
      cttccaata cccttccac ttttctcaa aggagtcac 
      tcttgtcgt ctcaccctt gattgcact gatgcaaga 
      ccaagtcct cgcactaac tgtttccaa tattccggc 
      gtgtctttt ggggtcggc gcaaaacga gcaaaggaa 
      agtcattga ggacatttt gcagaacaa gtacagtat 
      tgtttacat cacccctga gttctgttc aggcgactt 
      ggggcttga gcttttgaa aaaaatggc gcaacagct 
      caaaattgt cctgattgc gatcgatga ggcccattg 
      tattagctc ctgggggca tgattttag gtcgcagta 
      tcgcaattt gggaaattt gcgacagat ttttccgcg 
      ggttcctgt gatcgcagt cacggcgac tgcaacaac 
      cagagtgcg aggcgatat tgtcaaaag tctccaact 
      gcgggaccc tcttattgt ttgttccgg cttcgaccg 
      cccgaatct ctacttcga agtgtacca aagagcagg 
      cggtggcgc cttcaaaga cctgaccaa ggccatggt 
      ttacgaaaa atccgaatg gaaattctc tggtccaac 
      aattattta ttgtatcac ccgtagaca gactgaaga 
      tttatgcga aattttaaa aaattgtgg tgttaggtg 
      tcgagtgta tcacgccgg tttgtcgca aaaggaacg 
      tcaagaggc tcatgagga gtttgttcg cgacaaagt 
      cgacgtgat tgtggcaac aattgcctt cggcatggg 
      catagacaa gcctgatat tcgaaatgt gatacatta 
      cggctcatc taatagtgt cgaaagtta ctaccagga 
      agtaggccg tgctggacg cgacggact tccggcgcg 
      ctgtgtcac gatttacag ttccggcga ttttcaaat 
      cctccgaaa catcagcgg aggctcggt caaaaaaga 
      aacggcttt gaggcaaat tgaggatta tttgaccac 
      gcgcaagtg tcggcgccg cttcatttt ggagttttt 
      tgaggatga agtgggcga ggataagcc gaagcagag 
      gtgttgtga cgtgtgtac acaaaaact ttatgataa 
      tcgcaccga tcaggagaa atacgaagg tctggatga 
      aaaaggccg ttatgattt tacagaaga tgcgaaaaa 
      gtttctcaa aactgtcgc actttttgg tggaaagtg 
      cggtttgac cacttatat cctttttct gagagggtc 
      tcagtcgac aaggttgac tgaagataa aaggaaaca 
      tccgctgaa tggatgtgg gaaggacca atcggagga 
      atggtggaa gtgcatagc gaaaatcct cactgcgga 
      gaattgtct gagcgaaaa aaaccaaaa attatcatc 
      aggtttcac ctatcaagc cttatcatt aacaaacaa 
      aggacaatc gatgattaa cggaagtga gaaattact 
      catcccgcc accgtcaac tgtcctcac gttattgaa 
      acaaaagcc ggccaaaga atcggtttg gttaaccac 
      tcgaccggt taacacccc ctcaacgag taaacagcc 
      ggaaaatac cgaaactcc ggaggaaat tgagcaacg 
      gatgtcact ctaccggaa actactcga ccaaaggac 
      cgatattgc ggcaaaatt ggactgtat gccatacat 
      ggtggcctc aaacacagt cttaatgga aatgtcaaa 
      atgtaaacc gaaaacaat cgccgatct tcgaacctt 
      aaatcttga gggcttccc cgaggccaa aatactcaa 
      atttggggc cgcctttct gggggtcat tgcgagaca 
      actcaagtt gaataaacc aaaaattcg ggagattct 
      actccaaaa ccctttacc cgacgttag catgactgt 
      cactgccga agtctcgtg ctccatgct agagtcggg 
      gctcactgt gggcgaaat cgccgccaa aaggggggt 
      caccgtggg gacaattgc ctcgcacct catttccgg 
      cataaaatt cgggtttcc ggtgaaaat gtcggaact 
      gggggtcag tgaggagca tcgcgacgc cataataaa 
      agcctacag ggggcttgt ggggccccc cttaagtcc 
      agttttagg gtttaacag tgttaggtt gtccgagat 
      gaaggagag gtgcccccc ggattttac cttcgacca 
      gattaagac agttttggc gtatttgga ggtcaggtg 
      tcacttggc ggctctcaa ttttgacta cgaggagtt 
      tgaagactt taactttag tgatgttga tttgaagac 
      tgaaatgaa gagtgagga ggaggggcc ccctttgaa 
      acgcgagaa gaaagactg tgatccgtg tgtggacgc 
      aattttgga ttcgccccc cagggaagt caaagctga 
      gccaaaaaa atcgaaaaa ggcgctccc gccttggct 
      cgtgcccaa aaaattgtc ctaaattgt tattgttaa 
      gtcctaacc gttttttct tgttttttt ttatgaaat 
      aaaactatt tttttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY