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Gene information
General information
TC011773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13711188..13712759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011773
    iBeetle screen iB_07690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011773 RA
      MTTEERKSC LKSCFDAWK RLVKMRIEK SKAVQKKLE 
      QQEKLENLI KALKKVQKE EKTEPRTQP LKVLNNFKN 
      RFVAQKSTI ERLTSKLEQ KERLILELK LGILDKEAF 
      NESKVEIRE IFAKCSAKT RCKVAPPVD YSEKFMIMT 
      QKAPKFVQE LEERALERA KRHEVVLER KRQMDETRA 
      RIIAEALEK KRAQDEEEK KKYLEMMKE RRRLELERE 
      KKRQVNKAI FMEKMQKAE DLHNRHLLK NALKKLLDE 
      YIDMKMKQE KSELHLKIT ILSKSITAW KQFVEGKYR 
      DKNALADAC FARNALTRV MDKWRKLRH ERVQANQVA 
      EDYYDFVLL NKSFVQWHR HVCAEIIRQ DRKKKLADL 
      HYKRRILFH HYYMWRSLP AIIQLEKSK EEKMKKWRE 
      KVWEVLPDY KPPYDDD
    CDS sequence
    • >TC011773 RA
      atgaccacc gaagagcga aaatcgtgc ctcaagtcg 
      tgcttcgac gcctggaag cgactagtt aagatgcga 
      atcgagaaa agcaaagct gttcaaaaa aaattggaa 
      cagcaggag aaacttgaa aacttgatc aaagccttg 
      aaaaaggtc caaaaggag gagaagacc gaacctagg 
      acacagccc ttgaaggtg ctaaacaac ttcaagaat 
      cgcttcgtg gcccagaag agcaccatc gagcgcttg 
      acctcaaag ctcgaacag aaggagcgt ttgatcctc 
      gaactgaaa ctcggaatt ttggacaaa gaagccttc 
      aatgagagt aaagtcgaa attcgcgag attttcgcc 
      aagtgttcg gcgaaaacc cggtgcaaa gtggccccg 
      ccggtcgac tacagcgaa aagttcatg attatgacc 
      caaaaagcc ccaaagttc gtccaagag ttggaagaa 
      cgggcactt gaacgtgcc aaacgccac gaagtcgtg 
      ctggaacgg aagagacag atggacgag acgcgcgcg 
      aggatcata gccgaagcc ttggagaag aaacgggcg 
      caggacgaa gaagaaaag aagaaatac ctcgaaatg 
      atgaaagaa cgtcggaga ctggagctc gagcgtgag 
      aagaagcgg caagtgaac aaggcgatt ttcatggag 
      aaaatgcaa aaagccgaa gatttgcac aacaggcat 
      ttgctcaaa aacgccttg aagaagctc ctcgacgaa 
      tacatcgat atgaagatg aagcaggag aagagcgag 
      ctgcacctc aaaatcacc attttgagt aagtcaatc 
      acggcatgg aaacagttc gttgagggt aaataccgg 
      gataagaac gccctggct gatgcgtgt ttcgcgagg 
      aatgcgcta actcgggtg atggacaag tggcgcaaa 
      ctacgacat gaacgcgtt caggccaat caagtcgct 
      gaggattat tacgatttt gttttgctg aacaaaagt 
      ttcgtccag tggcatcgc cacgtctgt gccgagatt 
      ataaggcag gacagaaag aaaaaactg gccgacttg 
      cattacaaa aggcgaatt ttgtttcat cattactac 
      atgtggcgg tcgttgccg gcgattatc cagctggag 
      aagtccaag gaggagaag atgaagaag tggagggag 
      aaggtgtgg gaggtcttg cccgactac aagccccca 
      tatgatgac gattaa
    mRNA sequence
    • >TC011773 RA
      tgacgcgcc ctcagtcaa acgtcaccg cggcgccaa 
      attctaatt atcgccccc cttggcaac caaaactta 
      tcaaaacat tacagggtg tttgataca atgaccacc 
      gaagagcga aaatcgtgc ctcaagtcg tgcttcgac 
      gcctggaag cgactagtt aagatgcga atcgagaaa 
      agcaaagct gttcaaaaa aaattggaa cagcaggag 
      aaacttgaa aacttgatc aaagccttg aaaaaggtc 
      caaaaggag gagaagacc gaacctagg acacagccc 
      ttgaaggtg ctaaacaac ttcaagaat cgcttcgtg 
      gcccagaag agcaccatc gagcgcttg acctcaaag 
      ctcgaacag aaggagcgt ttgatcctc gaactgaaa 
      ctcggaatt ttggacaaa gaagccttc aatgagagt 
      aaagtcgaa attcgcgag attttcgcc aagtgttcg 
      gcgaaaacc cggtgcaaa gtggccccg ccggtcgac 
      tacagcgaa aagttcatg attatgacc caaaaagcc 
      ccaaagttc gtccaagag ttggaagaa cgggcactt 
      gaacgtgcc aaacgccac gaagtcgtg ctggaacgg 
      aagagacag atggacgag acgcgcgcg aggatcata 
      gccgaagcc ttggagaag aaacgggcg caggacgaa 
      gaagaaaag aagaaatac ctcgaaatg atgaaagaa 
      cgtcggaga ctggagctc gagcgtgag aagaagcgg 
      caagtgaac aaggcgatt ttcatggag aaaatgcaa 
      aaagccgaa gatttgcac aacaggcat ttgctcaaa 
      aacgccttg aagaagctc ctcgacgaa tacatcgat 
      atgaagatg aagcaggag aagagcgag ctgcacctc 
      aaaatcacc attttgagt aagtcaatc acggcatgg 
      aaacagttc gttgagggt aaataccgg gataagaac 
      gccctggct gatgcgtgt ttcgcgagg aatgcgcta 
      actcgggtg atggacaag tggcgcaaa ctacgacat 
      gaacgcgtt caggccaat caagtcgct gaggattat 
      tacgatttt gttttgctg aacaaaagt ttcgtccag 
      tggcatcgc cacgtctgt gccgagatt ataaggcag 
      gacagaaag aaaaaactg gccgacttg cattacaaa 
      aggcgaatt ttgtttcat cattactac atgtggcgg 
      tcgttgccg gcgattatc cagctggag aagtccaag 
      gaggagaag atgaagaag tggagggag aaggtgtgg 
      gaggtcttg cccgactac aagccccca tatgatgac 
      gattaatgc tttattcaa ttattcgac atttgttta 
      actgtccag gttcaacct aatttcttc tctttttga 
      ttgagtttt cagataata gttctaatt ttaatgaga 
      cttaattat tttcttttt cactaaagt taaatttaa 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY