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Gene information
General information
TC011798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13403234..13406223
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011798
    iBeetle screen iB_07686

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011798 RA
      MSSLTSEFS SVRKPKREG NLTEPPFSL STIKGRSCQ 
      RLKFMSIKP LRLDEIQKE IEEAENSAE RANVYCPPS 
      KEPRGCAPL QDSFDLNFS PLATPSKEP PCDKFVTPD 
      NRIPAKVQC SVRVKPALP GLSAGSPEV SFRRIRVNE 
      VGYVVLNEL GKGGSSQVF HCFNPETKQ HRAIKVVSL 
      ENKVSAVGF INEVKMLQT LQKCNRIIK MFDYEIKEE 
      EKKLLVVLE VGGTDLSKI LKESALNNA HMPLYMLLY 
      YWMEMLYAV RQIHESGIV HSDLKPANF LEVEGYLKL 
      IDFGISSCI QSDMTSVIK AVPEGSFNY ISPEALSSE 
      TSSNASSPS FGKPKYKIS YKSDVWSLG CILYQLVYR 
      RTPFQHITQ IWVKLSAIV NPDHKIDYP DAHWVPQKL 
      INTIKKCLQ RNAKARPSI DELITDYEN MFHSLV
    CDS sequence
    • >TC011798 RA
      atgagttct ctgactagt gagttttct tcagtgcgg 
      aagccgaaa cgggagggc aatttaacg gagccgccg 
      ttcagcctc agtacgatc aaagggcgg tcatgccag 
      cgcctcaag ttcatgtca ataaagccg ctgcggctt 
      gacgagatc cagaaagag atcgaagag gcggaaaac 
      agcgccgaa cgcgccaat gtgtactgc ccccccagc 
      aaggaaccc cggggttgc gcccccttg caggacagc 
      ttcgacctg aacttctcc ccgctggcc accccgtcc 
      aaggagccc ccgtgtgac aagttcgtg acccccgat 
      aacaggatc ccggccaag gtgcaatgc agtgtgagg 
      gtgaagccc gcactgccc ggtctgagc gccggctct 
      ccggaggtc tcgtttcgg agaattcgg gtcaatgag 
      gtggggtat gtggtgctg aatgagttg gggaaaggg 
      ggcagttcg caggttttt cactgtttt aaccccgag 
      actaagcaa catcgggcg attaaagtc gtttcactg 
      gagaataag gtctcggct gtggggttc atcaacgag 
      gtgaagatg ctgcagacg ttgcaaaag tgcaatcgc 
      atcattaag atgttcgat tatgagatt aaggaggaa 
      gagaagaag ttgttggtg gtgttggag gttggaggg 
      actgatctg tcgaagatt ttgaaagag tcggcgctc 
      aacaacgcc catatgccc ctctacatg ctgctctac 
      tactggatg gagatgttg tacgccgtt aggcaaatt 
      catgaaagc ggaatcgtt cattcggat ttgaagcca 
      gcgaatttt ctcgaagtg gaaggttat ttgaaactg 
      atcgatttc ggaatctcc agttgcatt cagagcgat 
      atgacgagt gtcataaag gcggtgcct gagggctcc 
      ttcaattac ataagtccc gaagcgcta agcagtgag 
      actagttcg aatgccagc agcccctcg ttcggaaaa 
      ccaaagtac aaaattagt tataagtct gatgtttgg 
      tcgctgggt tgcatctta taccagctg gtctaccgc 
      cgcaccccc ttccagcac atcacccag atctgggtg 
      aaactttcg gcaatcgtg aacccggac cacaaaatc 
      gactacccg gacgcccac tgggtgccc caaaaactg 
      ataaatacc ataaaaaag tgccttcaa cgtaacgca 
      aaagccagg ccctcgatc gacgaacta atcaccgac 
      tacgaaaac atgttccat agtttagtg taa
    mRNA sequence
    • >TC011798 RA
      caattagtg cggccacga gtaacatag cgttgcttt 
      accaataat acgatgaaa caccaacga tttgcccca 
      acaatgcgc gaaagttag ccgtattac gtttgagcg 
      cagtaactt tgtcgtttc ggttggcgc cccgccaac 
      atggcggaa aaccgttga aaaacaaat tcaaaaaat 
      gagttctct gactagtga gttttcttc agtgcggaa 
      gccgaaacg ggagggcaa tttaacgga gccgccgtt 
      cagcctcag tacgatcaa agggcggtc atgccagcg 
      cctcaagtt catgtcaat aaagccgct gcggcttga 
      cgagatcca gaaagagat cgaagaggc ggaaaacag 
      cgccgaacg cgccaatgt gtactgccc ccccagcaa 
      ggaaccccg gggttgcgc ccccttgca ggacagctt 
      cgacctgaa cttctcccc gctggccac cccgtccaa 
      ggagccccc gtgtgacaa gttcgtgac ccccgataa 
      caggatccc ggccaaggt gcaatgcag tgtgagggt 
      gaagcccgc actgcccgg tctgagcgc cggctctcc 
      ggaggtctc gtttcggag aattcgggt caatgaggt 
      ggggtatgt ggtgctgaa tgagttggg gaaaggggg 
      cagttcgca ggtttttca ctgttttaa ccccgagac 
      taagcaaca tcgggcgat taaagtcgt ttcactgga 
      gaataaggt ctcggctgt ggggttcat caacgaggt 
      gaagatgct gcagacgtt gcaaaagtg caatcgcat 
      cattaagat gttcgatta tgagattaa ggaggaaga 
      gaagaagtt gttggtggt gttggaggt tggagggac 
      tgatctgtc gaagatttt gaaagagtc ggcgctcaa 
      caacgccca tatgcccct ctacatgct gctctacta 
      ctggatgga gatgttgta cgccgttag gcaaattca 
      tgaaagcgg aatcgttca ttcggattt gaagccagc 
      gaattttct cgaagtgga aggttattt gaaactgat 
      cgatttcgg aatctccag ttgcattca gagcgatat 
      gacgagtgt cataaaggc ggtgcctga gggctcctt 
      caattacat aagtcccga agcgctaag cagtgagac 
      tagttcgaa tgccagcag cccctcgtt cggaaaacc 
      aaagtacaa aattagtta taagtctga tgtttggtc 
      gctgggttg catcttata ccagctggt ctaccgccg 
      caccccctt ccagcacat cacccagat ctgggtgaa 
      actttcggc aatcgtgaa cccggacca caaaatcga 
      ctacccgga cgcccactg ggtgcccca aaaactgat 
      aaataccat aaaaaagtg ccttcaacg taacgcaaa 
      agccaggcc ctcgatcga cgaactaat caccgacta 
      cgaaaacat gttccatag tttagtgta aataaaccc 
      tttatataa ttttataca aaaaattta ttacttgcc 
      tttacaaac actgaataa ataaaataa ggcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY