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Gene information
General information
TC034430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13095028..13098690
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034430
    iBeetle screen iB_07683

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034430 RA
      MSTLMWSCP CCLRFKFSP EEIEQRYKS LEIDKMLEK 
      DKQALKRQV KLLLLGAGE SGKSTFLKQ MKIIHGVKF 
      ESEMLMEYQ QIIYQNVIK GMRVLVDAR DKLGIPWGD 
      TSNAALGKE LLQFNASVL DSRTFIQYC PLLVKLWRD 
      SGILRAFDR RREFQLSDS VEYFLNNLD RVSRHDYIP 
      NHKDILHCR KATKGITEF AIPINNIPF LFVDVGGQR 
      SQRQKWFQC FDSVTSILF LVSSSEFDQ VLLEDRKTN 
      RLEESRDIF DTIVNNRIF VNVSVILFL NKYDLLLKK 
      VANPETNIH WYFPQFAGN PHSINDVKE FILAMFTGV 
      KRESRKVLY HHFTTAVDT ENIKVVFEY VKEIILHRN 
      LESLLL
    CDS sequence
    • >TC034430 RA
      atgtccact ttaatgtgg tcctgtccg tgttgttta 
      cggttcaag ttcagcccc gaagagatc gagcagcgg 
      tacaagtcg ttagaaatc gacaaaatg ctcgagaag 
      gacaagcaa gccctcaaa cggcaagtc aagctgctg 
      ttattgggc gctggggag agtggcaag tcgactttt 
      ctcaagcag atgaagatc atccacggt gtgaagttc 
      gagagcgaa atgctgatg gagtaccag cagatcatc 
      taccagaac gtcatcaag gggatgcgg gtgctggtg 
      gacgccaga gacaagctg ggcatcccg tggggcgac 
      acctcgaac gccgccctc ggcaaggag ctgctgcag 
      ttcaacgcc tcggtcctg gactccaga accttcatc 
      cagtattgc cctttgctg gtcaagctg tggcgggat 
      agcggcatt ttaagggct tttgatagg agaagggag 
      tttcagttg agtgactct gtggaatat ttcctgaac 
      aatttggac cgtgtctca cgtcacgac tacatcccc 
      aatcacaag gacattttg cactgccgt aaagccacc 
      aagggaatc accgaattt gcgattccg ataaacaat 
      attccgttt ttgttcgtc gacgtcggc ggtcaaaga 
      tcgcagaga caaaagtgg tttcagtgt ttcgattcg 
      gtgacgtcg atactgttc ctcgtgtcg tcgtccgaa 
      ttcgaccaa gtgctgctc gaggacagg aaaacgaac 
      cgcttggag gagtcgaga gatattttc gacacgata 
      gtcaacaat cggatattt gtgaacgtc tccgtgatc 
      ctctttctg aacaaatac gacctgttg ttgaagaag 
      gtggccaat cccgagacg aacatccac tggtacttc 
      ccccagttt gcggggaat ccccactcg atcaacgac 
      gtgaaggag ttcattctg gcgatgttc acgggggtg 
      aagagggag tcgcggaag gtgctctat caccacttt 
      acgacggcc gttgatacg gaaaatatc aaagtcgtt 
      ttcgaatac gtaaaagag attattctg catcgaaat 
      ctggaaagt cttctgctc taa
    mRNA sequence
    • >TC034430 RA
      taccaacca ttcagtcat ttccaccgc acagctatg 
      tagtggact agttgtggt gttttatta aaaatgtcc 
      actttaatg tggtcctgt ccgtgttgt ttacggttc 
      aagttcagc cccgaagag atcgagcag cggtacaag 
      tcgttagaa atcgacaaa atgctcgag aaggacaag 
      caagccctc aaacggcaa gtcaagctg ctgttattg 
      ggcgctggg gagagtggc aagtcgact tttctcaag 
      cagatgaag atcatccac ggtgtgaag ttcgagagc 
      gaaatgctg atggagtac cagcagatc atctaccag 
      aacgtcatc aaggggatg cgggtgctg gtggacgcc 
      agagacaag ctgggcatc ccgtggggc gacacctcg 
      aacgccgcc ctcggcaag gagctgctg cagttcaac 
      gcctcggtc ctggactcc agaaccttc atccagtat 
      tgccctttg ctggtcaag ctgtggcgg gatagcggc 
      attttaagg gcttttgat aggagaagg gagtttcag 
      ttgagtgac tctgtggaa tatttcctg aacaatttg 
      gaccgtgtc tcacgtcac gactacatc cccaatcac 
      aaggacatt ttgcactgc cgtaaagcc accaaggga 
      atcaccgaa tttgcgatt ccgataaac aatattccg 
      tttttgttc gtcgacgtc ggcggtcaa agatcgcag 
      agacaaaag tggtttcag tgtttcgat tcggtgacg 
      tcgatactg ttcctcgtg tcgtcgtcc gaattcgac 
      caagtgctg ctcgaggac aggaaaacg aaccgcttg 
      gaggagtcg agagatatt ttcgacacg atagtcaac 
      aatcggata tttgtgaac gtctccgtg atcctcttt 
      ctgaacaaa tacgacctg ttgttgaag aaggtggcc 
      aatcccgag acgaacatc cactggtac ttcccccag 
      tttgcgggg aatccccac tcgatcaac gacgtgaag 
      gagttcatt ctggcgatg ttcacgggg gtgaagagg 
      gagtcgcgg aaggtgctc tatcaccac tttacgacg 
      gccgttgat acggaaaat atcaaagtc gttttcgaa 
      tacgtaaaa gagattatt ctgcatcga aatctggaa 
      agtcttctg ctctaacac tgactgaag tgtgctatg 
      gctgtgtat cacacaatc gattttcgc aagactgac 
      taagtttaa ttagtctga taagacttt gggaatttt 
      aagtgtaca agaaattac acaaatgtg atactactt 
      tttttagaa tttttcgga gagggcaac caaagattc 
      aaattgcaa taaccaaat actcacatt tctgtaatt 
      ttgaccgaa tatatttat caatcgtag ttaggaata 
      ctgccaaac gtgcctcat ttttacgta tttatcgaa 
      ggttttaca gtccgccgg gttcttatg taaaatatt 
      gtaatatat tttttatat tttaggttt tagtaatgt 
      gatccgatt ttagaacgg cctaaatta tcgatagtt 
      tgtatatag tcacatatt ttgtgaaat tttaccgat 
      atttttcgg cagctgtgt gtctggtga attatttac 
      agggagttt tatagtgcc ttaaaacgc atcttctaa 
      aaatatact caatacaac ataaacaac gtaattctc 
      gaaacagtt atctgattt tcattgtat aataaaagt 
      gtatataag ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY