Status
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Gene information
General information
TC011826 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13079447..13087587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011826
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07682

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Antenna is the most sensitive part
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011826 RA
      MEGASKQPD EEIALKPKM TLVNGITVI VGSIIGSGI 
      FVSPAGVLM HTGSVNVSL LVWTISGIF SMVGAYCYA 
      ELGTMIRKS GADYAYIME TFGPFLAFI RLWIECMIV 
      RPCSQAIVA LTFSVYVMK PFFPECQPP EDAARLLAV 
      CCICVLTFV NCYDVKWAT RVQDVFTYA KLLALFIII 
      AAGGYQLVN GHTEHFSFI NTKSEVTSL ALSFYSGLF 
      AYNGWNYLN FIIEELKDP VRNLPLAIA ISCSLVTIV 
      YVLTNVAFY TTLSPAELL GSEAVAVSF ANRLFGPFA 
      WTIPVFVAL STFGAVNGI LLTSSRLFY SGACHGQMP 
      EILTMIQAQ RVTPTPSVL IMALLSMLY LTVSDIFAL 
      INYVGFATW LSIGVSVLC VPWLRWTHP DLERPIKVN 
      LIWPIIYLL ATVFVTAVP MVASPVETG IGMLMILTS 
      VPVYLVFIY WKNKPLWFL KATNSITIT LQQLLVVVG 
      KAKAANL
    CDS sequence
    • >TC011826 RA
      atggagggt gccagcaag cagcccgac gaggagatc 
      gccctcaag cccaagatg accctcgtc aacggcatc 
      accgtcatc gtgggctcc atcatcggc tccgggatc 
      ttcgtgtcg ccggcgggc gtcctcatg cacaccggc 
      agcgtcaac gtctccctc ctggtgtgg accatttcg 
      gggattttt tccatggtc ggcgcctac tgctacgcc 
      gaacttggc accatgatc aggaaaagc ggcgctgat 
      tacgcctac atcatggaa accttcggc cctttcctt 
      gccttcatc cgcctatgg atcgaatgc atgatagta 
      cggccgtgt tctcaagca atagtagcc ttaactttc 
      agtgtttac gtgatgaaa cccttcttc cctgaatgc 
      caaccacct gaagacgcc gcacgtttg ctcgccgtt 
      tgttgcatc tgtgtttta acgttcgtt aactgctac 
      gacgtgaag tgggcgact cgcgtccag gacgtcttc 
      acctacgcc aaactcctg gccctgttc atcatcata 
      gcggcagga gggtatcag ctggtcaac gggcacacg 
      gagcacttt tcattcatc aacacaaag agcgaagtt 
      acgtcgctg gcattatca ttctattcc ggcttgttc 
      gcctacaat ggctggaat tacctgaac tttattatc 
      gaggaactc aaggatcca gtcagaaat ctcccgctt 
      gctatcgca atttcgtgt tctttggta acaatcgtc 
      tacgttttg accaacgtg gcgttctac acaacgctt 
      tcacccgcc gagcttttg gggtctgaa gcagtggcg 
      gtgtcgttc gccaatcga ctttttggt cctttcgcg 
      tggacgatt cctgttttt gtggccctg tcgacgttt 
      ggggccgtt aatggtatt ttactgaca tcgtcacga 
      ctgttctat tcgggggct tgccatgga caaatgccc 
      gaaattctg accatgatt caggcgcag agggtcacc 
      cccacaccc tcggtgctc attatggcg ctgttatcg 
      atgctctat ctcacagta tcagacatt ttcgcttta 
      ataaactac gtcggcttc gccacatgg ctgagcatt 
      ggggtgtcc gtgctgtgc gtcccctgg ctgcgctgg 
      acgcacccg gacctggag cgccccatc aaagtgaac 
      ttaatctgg cccatcatc tacttactc gccaccgtt 
      ttcgtaaca gccgtgccc atggtcgcc agtcctgtc 
      gaaaccggc atcggcatg ctgatgatt ttgacatcg 
      gtaccagtt tatttggta tttatttat tggaagaac 
      aagccgctg tggttcctt aaggccacc aacagcata 
      accatcacg ctccagcag ctgctggtg gtggtgggc 
      aaagccaaa gcggccaac ttgtag
    mRNA sequence
    • >TC011826 RA
      aatggtttt gttaaaaaa acttagtct tagacttag 
      cgcaccaaa tgactaaat tgccacttc ctcaaataa 
      tctcgtcgc attttcgtc gacaaaacc cactcactc 
      caccacact caagtcaaa gtttgaaaa atcgagata 
      acacccgtg aggtcgcct cacttcttg ttttatctc 
      gcatgtttt ggtaaacat cgtttcata acgttcgaa 
      ttttcgaaa tgtccacgc gacgttgct ttagggcat 
      agtagggag cgaatggtc cagatattg atcgttctc 
      ctcctgcga gtgagtcgc taagacgcg gggttggcg 
      cacacacaa ccaagctct cggccatgg agggtgcca 
      gcaagcagc ccgacgagg agatcgccc tcaagccca 
      agatgaccc tcgtcaacg gcatcaccg tcatcgtgg 
      gctccatca tcggctccg ggatcttcg tgtcgccgg 
      cgggcgtcc tcatgcaca ccggcagcg tcaacgtct 
      ccctcctgg tgtggacca tttcgggga ttttttcca 
      tggtcggcg cctactgct acgccgaac ttggcacca 
      tgatcagga aaagcggcg ctgattacg cctacatca 
      tggaaacct tcggccctt tccttgcct tcatccgcc 
      tatggatcg aatgcatga tagtacggc cgtgttctc 
      aagcaatag tagccttaa ctttcagtg tttacgtga 
      tgaaaccct tcttccctg aatgccaac cacctgaag 
      acgccgcac gtttgctcg ccgtttgtt gcatctgtg 
      ttttaacgt tcgttaact gctacgacg tgaagtggg 
      cgactcgcg tccaggacg tcttcacct acgccaaac 
      tcctggccc tgttcatca tcatagcgg caggagggt 
      atcagctgg tcaacgggc acacggagc acttttcat 
      tcatcaaca caaagagcg aagttacgt cgctggcat 
      tatcattct attccggct tgttcgcct acaatggct 
      ggaattacc tgaacttta ttatcgagg aactcaagg 
      atccagtca gaaatctcc cgcttgcta tcgcaattt 
      cgtgttctt tggtaacaa tcgtctacg ttttgacca 
      acgtggcgt tctacacaa cgctttcac ccgccgagc 
      ttttggggt ctgaagcag tggcggtgt cgttcgcca 
      atcgacttt ttggtcctt tcgcgtgga cgattcctg 
      tttttgtgg ccctgtcga cgtttgggg ccgttaatg 
      gtattttac tgacatcgt cacgactgt tctattcgg 
      gggcttgcc atggacaaa tgcccgaaa ttctgacca 
      tgattcagg cgcagaggg tcaccccca caccctcgg 
      tgctcatta tggcgctgt tatcgatgc tctatctca 
      cagtatcag acattttcg ctttaataa actacgtcg 
      gcttcgcca catggctga gcattgggg tgtccgtgc 
      tgtgcgtcc cctggctgc gctggacgc acccggacc 
      tggagcgcc ccatcaaag tgaacttaa tctggccca 
      tcatctact tactcgcca ccgttttcg taacagccg 
      tgcccatgg tcgccagtc ctgtcgaaa ccggcatcg 
      gcatgctga tgattttga catcggtac cagtttatt 
      tggtattta tttattgga agaacaagc cgctgtggt 
      tccttaagg ccaccaaca gcataacca tcacgctcc 
      agcagctgc tggtggtgg tgggcaaag ccaaagcgg 
      ccaacttgt aggtagtga gtgcgaaag accaaagat 
      tacttatgc ctacatcga caataaatg tttgtatgc 
      atggaaaat aaactatgt tacgattta cgcggtttt 
      taacttgaa ccacggaga actatgtgc taggaagcg 
      aaataaggt acaaaaatg tccccgacg acagattct 
      attgttttt ttttatttg atagcgcag ttgttccgc 
      tactatgaa aaaaataaa aattgaaaa aactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY