| General information | |||
| TC031602
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452079.1: 0..6283 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031602 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07673 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031602 RA
MCRIVEKLR PERCHRCLK YGHRAKECK EKAGENNTE KGGRCLKCG RWGHHAKAC QNEPHCYEC EQQGHRADS MACPKMAHA MLAKLTHEL EASVVTVSE PNLTLAEKE AWLVDDTGS AAIKITDRS LNITDSGNG SSFAWARTG DMTYISCYI SPNISAAAF ETRLQELEA FLRTHHGNK LLTGDFNAA NRAWGSSTN DRRGQLVIE MTEALGMTL LNTGHSPTF LGSGGTSIV DLAFIDDSA AGRVRNWTV LEAETMSDH RAIAIDIED HRARPATGD HRYGGKAHA LRWTKDRDK QLREQLAEI LPRINTVDP ISVSRAIGE AAQRSLKKI ANNRKPIFW WTDEVGNAR QRCNAARRR AGRARDDRK TELWRIYKD ERKALKKEI KKAKAEAWQ RLIEDIEHD PWGKGYRIA IGRAAPRQQ VSERERWRA ARELFPQCA IPTYDMQEA CEPTMFSYE EIQAAAARM RMGKAAGPD GLGPEIVQA AVAASPETI SGLVNDLLK SQKFPEQWK RARLALIPK AKKSPDKLP KYRPICVLD SLGKFYEQL ILARLLGEL EAKGGLSDS QYGFVRGRS TIDAMQDVL RVVEWCSTG SYGRRELCA LILLDVANA FNSVRWPDI IQALIDKGI SAYLVNLVR SYLTDRFII VQHGDGFNA TCGVPQGSV LGPLLWNLV YDEVLHLDV PVGVRLFAF ADDLAMVAM GMTVDELQN NANLALDTV NRWMCSKHL TLAAEKTEA VIMTGRRDH ADIRFTVGD SVVKPSESA RYLGVYMDR KRTFAQHIA EAAAGTTKL TGKLSRILP NLRGASEGR RRTIAAAVT SKMMYGSEI WSKGLDKLS NAEKVNRAQ RPIMLRVAC AYRTVATTS LQVLCSMVP WDLLALERR ATFKRPENR KAEREKTIR QWKDRWDKE SRHSWTKRL IPEIEPWLT RRHGLLDFY TTQMMTGHG CFSSYLSRI GREATEDCW YCDCTRDDA EHTFFDCPR WTEERSEVE LILGAFPEP EALVGEMLK DERTKREGE RAQAPDAEA PLADGIDNS EQPTRSNVL KDSPGSAAT LLMRKRGLV GESPTLSGP LRTYHQRPG GRLLELPLN LGKKKKKKK VRSGQVRSG QVRSGPRVK FGWSSIGRL PRWRIGESL PDMGGTGEI KYPGRTKTS QDHTVTPDV VWKRGPRGF GAARKKLTV QGRDTLVPV GEGVVGQAL PDGQPTTVQ TPSVRSQAE ASPKATDFK RGSRQTLYE KISRSHSLG SADKKRKYT DMSPGYERS IEPYKKVES PEAFVLTEA LDMVIKLGL DLERRIENN TKREIKDLS ARLKRQVEI LSRTSVRAW VESHRYIEP EKIQIDVDI QTEALGEGH ADELARKEE THNEEILSL RSEVERLRQ ENEELRRMN RQPQEKTPH DQTTPQVYE GLKENVTAE NFEKLIDLK WTNQAYAKT TFVRNDAVK CEDQVVVVE AASGEDVAE RLTTRFPEI APATATSVP TPGTALCVT KSRTIELEN GERVEDIIG RSILLNIGK DHQNRRKNL MNGMRKVAT LASNKGIKK FRLYSLIDE PNSQKFETR KITEWALYD TEMMAEVYV PRARNRKST AGASEPEPE SEEWEEQKT KRRRTTKTI TVSKAETDE QTYASMLKR LKGEINVEE IGVTVKGVY ETSDGRIKI NLTEKGKGN CEKFRTAIE EKLGNKAEA KITTARKEL IITGIDKET TEEEVRLAI QQHYTEKTA CPEDTQVRS FHSNERTNK QTATVLVPE TEALHLLQK RKVIIGWTM CRIVEKLRP ERCHMCLKY GHRAKECKE KAGENNTEK GGRCLKCGR WGHHAKACQ NEPHCYECE QQGHRADSM ACPKYRILV EKMRRSTGA NNTSTKRRE
- >TC031602 RA
atgtgcaga atagtagag aagctgaga ccagagaga tgccatagg tgtctcaag tacggccac cgcgctaaa gaatgtaaa gagaaagcc ggcgaaaac aacaccgag aagggaggc agatgctta aagtgcggg cgctggggg caccacgca aaagcctgt caaaacgaa ccccactgt tacgaatgc gaacagcaa gggcatagg gccgattcc atggcctgc ccgaagatg gcccacgct atgctggcc aaactgaca cacgaattg gaggcgtcg gtcgttacg gtaagcgaa cctaactta acccttgcc gaaaaggaa gcatggctc gttgacgac acagggagc gcagctata aaaataact gatcggagt ttaaacatc accgacagt ggaaatggg tcgagtttt gcctgggcc aggactggc gacatgacc tacatcagc tgctacatc tcgccgaac atctcggca gcggcgttt gagacaaga ctccaagag ctggaagca ttcctcagg actcatcac ggcaacaaa ctgctcact ggcgacttc aacgcagcg aaccgagcg tggggatcg tcgacgaat gacaggagg gggcaactc gtcatcgag atgacggag gcgttggga atgaccctc cttaacacc ggacactcc ccaacattt ctcggttcg gggggaacg tccatagtc gacttggcg ttcatcgac gatagtgcg gcaggtagg gtgaggaac tggaccgtt ctcgaagcg gaaaccatg agcgaccac cgagcgatc gccatcgac atcgaagac cacagggcc agacccgcg acgggcgac catagatac ggaggaaag gcgcacgcg ctcagatgg actaaagac cgagataag cagctccgg gaacaactg gcggaaatt ctacccaga attaatacg gtcgacccg atctcagtg agccgggcc attggcgag gctgcccag cgctccctc aagaaaatc gctaataac aggaaaccc atcttctgg tggacggac gaagttggg aacgcgagg caaagatgt aacgccgca cgacgacgt gcgggaagg gcgagggac gaccgcaaa accgagcta tggcggatc tacaaggac gagaggaaa gcactgaag aaggaaata aagaaagct aaggcagag gcctggcaa aggcttata gaagacatc gaacacgac ccatggggt aagggctat agaatagca attgggagg gcggcccct cgacaacaa gtcagcgag cgagaaagg tggagggcg gcgcgtgaa ctgtttcct cagtgcgcc attcccacg tacgacatg caagaggcc tgcgaaccg acaatgttc tcctacgaa gagatacag gccgcggcg gcgagaatg cgcatgggc aaggcggcc ggacctgac ggcctgggc cccgagata gtgcaagct gctgtagcg gcgtctccc gagaccatt tctggactc gtcaacgac ttattaaaa agtcaaaag ttcccggaa cagtggaag agggccaga ttggcactc atacccaag gcaaagaag tcgcccgac aaactaccc aaatatcgc cctatatgc gtgctggat agcctgggg aagttctat gaacaacta atacttgcc agactgctc ggcgagctc gaagccaag ggcgggctc tccgactcc cagtacggc ttcgtgaga ggcaggtcg accattgac gcgatgcag gatgtactc agggtggtc gagtggtgc tccaccggc tcgtacggc agaagggag ctgtgtgcc ctaatattg ctcgacgtc gccaacgca ttcaatagc gtcaggtgg cccgacatt attcaggca ttgattgac aaagggata agtgcgtac ctcgtcaac ctggtgcgc tcgtatctc accgatcga ttcatcatc gtgcagcat ggcgacgga ttcaacgcg acatgcgga gtgccccag gggtcggta ctgggcccg ctcctctgg aatctcgtt tacgacgag gtgctacat ctcgacgtt cccgttggc gtgcgcctt ttcgccttc gccgacgac ctggcgatg gtcgccatg gggatgacg gttgacgag ttacaaaat aatgcgaac cttgccctc gacactgtg aaccgatgg atgtgtagc aaacactta acgctagcg gcggagaag accgaggcc gttataatg acgggcagg agagaccac gctgacatc aggttcact gtcggcgac agtgtggta aaacccagc gagagcgcc agatacctg ggggtatac atggacagg aaaagaacg ttcgcccaa cacatagcg gaagccgct gcggggacc accaagttg acgggcaag ctatcaagg attctcccg aatctccgg ggagcctcg gaggggaga cggaggacg attgcagct gcggtcacc agcaaaatg atgtacggg tccgagata tggtccaaa gggctggac aaattaagc aacgccgag aaagtaaac agggctcag agaccaata atgttaagg gttgcgtgc gcctaccgc actgtggcg accacctct ctccaagta ctgtgttcc atggtgccg tgggacttg ctggcactg gagaggagg gcgaccttc aagagaccc gaaaacagg aaagcggaa agggaaaaa actattcga cagtggaag gataggtgg gacaaggag agccggcac agctggacc aagaggctt attcccgag attgagccc tggctgacg aggaggcac ggcctgcta gacttttac accacacag atgatgacc ggccacggc tgcttttcc tcctacctg tctaggata gggagggag gcaaccgag gactgctgg tactgcgac tgcacgaga gacgacgct gaacataca ttttttgat tgcccaagg tggacggag gaaaggagc gaagtcgaa ctaatattg ggggcattc cccgaaccg gaagcactc gtaggggaa atgctaaag gatgagagg acgaagaga gaaggagaa agggcgcag gcgccagac gctgaggct cccctagca gatggcata gacaacagc gaacagccg acccgaagt aatgtcttg aaggatagt cccgggtcg gctgccaca ttgctgatg aggaaaagg ggtttagtg ggtgagagc cccacactc tccgggccg ctaagaacg taccaccaa aggcccggg ggtcggctg ttagagctt cccctcaac ctcggtaaa aaaaaaaaa aaaaaaaag gtcaggtca ggtcaggtc aggtcaggt caggtcagg tcaggtccc cgtgtgaag tttggctgg tcttccatc gggcgcctc cccaggtgg cggataggg gaaagcctc ccagatatg ggtggtacc ggggaaatc aaatacccg gggcggacc aaaaccagc caagatcac accgtaact ccagacgtg gtctggaaa cgggggccg cgcggtttc ggggctgcg aggaagaaa cttacggtg caagggagg gatacccta gtacccgtc ggtgaggga gtggttgga caggctctc cctgacggt cagccaacg actgttcag acaccttcg gtgaggagt caggccgag gcgtcaccg aaagctact gacttcaaa cgaggaagc aggcaaaca ctgtacgag aaaatatca agaagccac tcgctagga agtgccgat aagaaacgg aagtacacg gatatgtct ccaggctac gaacggagc atagaaccc tacaaaaag gttgagagt ccagaggcc tttgtgcta acagaagca ctggacatg gtaattaaa ctaggtttg gatcttgaa aggagaatt gaaaacaac acgaaaagg gaaatcaag gacctatcg gcaagactc aaaaggcag gtggagatt cttagtcga acttctgtg agggcgtgg gtcgaatca cacagatac attgagccc gaaaaaata caaattgat gtcgacata cagaccgag gcgttaggc gaagggcac gctgatgaa ctagcccgg aaagaagaa acacacaac gaagaaatt ctctcactg cggtccgaa gtagagaga ctgagacaa gagaacgaa gagctgaga agaatgaac agacaacca caggagaaa acaccgcac gaccagacc accccacaa gtatatgag ggtcttaag gaaaacgta actgccgag aacttcgaa aaactgatc gacctgaaa tggacgaat caagcatac gcaaagacg acctttgta cgcaacgac gcagtgaaa tgcgaagac caggtggtc gtcgtcgaa gcggcaagc ggagaggat gtcgcagaa aggctcacg actaggttt ccggaaatc gcaccagcg acggcgacg agtgttcct acaccggga accgcgcta tgtgtgacg aaatccagg accatcgaa ctcgagaat ggcgaaagg gtagaagac ataatcggg cgctctata ctacttaac ataggcaag gatcatcag aatcgaagg aagaaccta atgaacgga atgcgcaaa gtagccacc ctggcaagt aacaagggg atcaaaaag tttcggcta tattcgttg atcgatgaa ccgaatagc caaaagttt gaaacgaga aagattact gaatgggcc ctatacgac acggagatg atggctgaa gtatatgtc ccgcgagca aggaataga aaatccaca gcgggagcg agtgaaccc gaaccagag agtgaagaa tgggaagag caaaagact aaacgacgc agaaccacc aaaaccata accgtaagc aaagctgag accgacgag caaacctac gccagcatg ctgaaacgg ctcaagggc gagatcaac gtggaagag atcggtgtc acggtcaag ggtgtctac gagacgagc gacggtaga atcaagatc aacctgacc gaaaaaggg aagggaaac tgcgagaaa tttaggacg gccattgaa gagaaactc ggaaacaag gctgaagca aagataacc acggcgagg aaagaactg ataataacg ggcatcgac aaagagacc acagaagag gaggtacgc ctagcgata caacaacat tacaccgaa aaaacggca tgcccagaa gatacgcag gtgcgttcg ttccattct aacgagcga acgaacaaa cagacggcg acggtgcta gtgccggag accgaggca ttgcatctc ctacaaaag aggaaggtc ataatcggg tggactatg tgcagaata gtagagaag ctgagacca gagagatgc catatgtgt ctcaagtac ggccaccgc gctaaagaa tgtaaagag aaagccggc gaaaacaac accgagaag ggaggcaga tgcttaaag tgcgggcgc tgggggcac cacgcaaaa gcctgtcaa aacgaaccc cactgttac gaatgcgaa cagcaaggg catagggcc gattccatg gcctgcccg aagtatcga atacttgtg gaaaagatg aggaggagt acaggtgcg aataacacc tctacaaaa agacgagag tga
- >TC031602 RA
cgagcgaac gaacaaaca gacggcgac ggtgctagt gccggagac cgaggcatt gcatctcct acaaaagag gaaggtcat aatcgggtg gactatgtg cagaatagt agagaagct gagaccaga gagatgcca taggtgtct caagtacgg ccaccgcgc taaagaatg taaagagaa agccggcga aaacaacac cgagaaggg aggcagatg cttaaagtg cgggcgctg ggggcacca cgcaaaagc ctgtcaaaa cgaacccca ctgttacga atgcgaaca gcaagggca tagggccga ttccatggc ctgcccgaa gatggccca cgctatgct ggccaaact gacacacga attggaggc gtcggtcgt tacggtaag cgaacctaa cttaaccct tgccgaaaa ggaagcatg gctcgttga cgacacagg gagcgcagc tataaaaat aactgatcg gagtttaaa catcaccga cagtggaaa tgggtcgag ttttgcctg ggccaggac tggcgacat gacctacat cagctgcta catctcgcc gaacatctc ggcagcggc gtttgagac aagactcca agagctgga agcattcct caggactca tcacggcaa caaactgct cactggcga cttcaacgc agcgaaccg agcgtgggg atcgtcgac gaatgacag gagggggca actcgtcat cgagatgac ggaggcgtt gggaatgac cctccttaa caccggaca ctccccaac atttctcgg ttcgggggg aacgtccat agtcgactt ggcgttcat cgacgatag tgcggcagg tagggtgag gaactggac cgttctcga agcggaaac catgagcga ccaccgagc gatcgccat cgacatcga agaccacag ggccagacc cgcgacggg cgaccatag atacggagg aaaggcgca cgcgctcag atggactaa agaccgaga taagcagct ccgggaaca actggcgga aattctacc cagaattaa tacggtcga cccgatctc agtgagccg ggccattgg cgaggctgc ccagcgctc cctcaagaa aatcgctaa taacaggaa acccatctt ctggtggac ggacgaagt tgggaacgc gaggcaaag atgtaacgc cgcacgacg acgtgcggg aagggcgag ggacgaccg caaaaccga gctatggcg gatctacaa ggacgagag gaaagcact gaagaagga aataaagaa agctaaggc agaggcctg gcaaaggct tatagaaga catcgaaca cgacccatg gggtaaggg ctatagaat agcaattgg gagggcggc ccctcgaca acaagtcag cgagcgaga aaggtggag ggcggcgcg tgaactgtt tcctcagtg cgccattcc cacgtacga catgcaaga ggcctgcga accgacaat gttctccta cgaagagat acaggccgc ggcggcgag aatgcgcat gggcaaggc ggccggacc tgacggcct gggccccga gatagtgca agctgctgt agcggcgtc tcccgagac catttctgg actcgtcaa cgacttatt aaaaagtca aaagttccc ggaacagtg gaagagggc cagattggc actcatacc caaggcaaa gaagtcgcc cgacaaact acccaaata tcgccctat atgcgtgct ggatagcct ggggaagtt ctatgaaca actaatact tgccagact gctcggcga gctcgaagc caagggcgg gctctccga ctcccagta cggcttcgt gagaggcag gtcgaccat tgacgcgat gcaggatgt actcagggt ggtcgagtg gtgctccac cggctcgta cggcagaag ggagctgtg tgccctaat attgctcga cgtcgccaa cgcattcaa tagcgtcag gtggcccga cattattca ggcattgat tgacaaagg gataagtgc gtacctcgt caacctggt gcgctcgta tctcaccga tcgattcat catcgtgca gcatggcga cggattcaa cgcgacatg cggagtgcc ccaggggtc ggtactggg cccgctcct ctggaatct cgtttacga cgaggtgct acatctcga cgttcccgt tggcgtgcg ccttttcgc cttcgccga cgacctggc gatggtcgc catggggat gacggttga cgagttaca aaataatgc gaaccttgc cctcgacac tgtgaaccg atggatgtg tagcaaaca cttaacgct agcggcgga gaagaccga ggccgttat aatgacggg caggagaga ccacgctga catcaggtt cactgtcgg cgacagtgt ggtaaaacc cagcgagag cgccagata cctgggggt atacatgga caggaaaag aacgttcgc ccaacacat agcggaagc cgctgcggg gaccaccaa gttgacggg caagctatc aaggattct cccgaatct ccggggagc ctcggaggg gagacggag gacgattgc agctgcggt caccagcaa aatgatgta cgggtccga gatatggtc caaagggct ggacaaatt aagcaacgc cgagaaagt aaacagggc tcagagacc aataatgtt aagggttgc gtgcgccta ccgcactgt ggcgaccac ctctctcca agtactgtg ttccatggt gccgtggga cttgctggc actggagag gagggcgac cttcaagag acccgaaaa caggaaagc ggaaaggga aaaaactat tcgacagtg gaaggatag gtgggacaa ggagagccg gcacagctg gaccaagag gcttattcc cgagattga gccctggct gacgaggag gcacggcct gctagactt ttacaccac acagatgat gaccggcca cggctgctt ttcctccta cctgtctag gatagggag ggaggcaac cgaggactg ctggtactg cgactgcac gagagacga cgctgaaca tacattttt tgattgccc aaggtggac ggaggaaag gagcgaagt cgaactaat attgggggc attccccga accggaagc actcgtagg ggaaatgct aaaggatga gaggacgaa gagagaagg agaaagggc gcaggcgcc agacgctga ggctcccct agcagatgg catagacaa cagcgaaca gccgacccg aagtaatgt cttgaagga tagtcccgg gtcggctgc cacattgct gatgaggaa aaggggttt agtgggtga gagccccac actctccgg gccgctaag aacgtacca ccaaaggcc cgggggtcg gctgttaga gcttcccct caacctcgg taaaaaaaa aaaaaaaaa aaaggtcag gtcaggtca ggtcaggtc aggtcaggt caggtcagg tccccgtgt gaagtttgg ctggtcttc catcgggcg cctccccag gtggcggat aggggaaag cctcccaga tatgggtgg taccgggga aatcaaata cccggggcg gaccaaaac cagccaaga tcacaccgt aactccaga cgtggtctg gaaacgggg gccgcgcgg tttcggggc tgcgaggaa gaaacttac ggtgcaagg gagggatac cctagtacc cgtcggtga gggagtggt tggacaggc tctccctga cggtcagcc aacgactgt tcagacacc ttcggtgag gagtcaggc cgaggcgtc accgaaagc tactgactt caaacgagg aagcaggca aacactgta cgagaaaat atcaagaag ccactcgct aggaagtgc cgataagaa acggaagta cacggatat gtctccagg ctacgaacg gagcataga accctacaa aaaggttga gagtccaga ggcctttgt gctaacaga agcactgga catggtaat taaactagg tttggatct tgaaaggag aattgaaaa caacacgaa aagggaaat caaggacct atcggcaag actcaaaag gcaggtgga gattcttag tcgaacttc tgtgagggc gtgggtcga atcacacag atacattga gcccgaaaa aatacaaat tgatgtcga catacagac cgaggcgtt aggcgaagg gcacgctga tgaactagc ccggaaaga agaaacaca caacgaaga aattctctc actgcggtc cgaagtaga gagactgag acaagagaa cgaagagct gagaagaat gaacagaca accacagga gaaaacacc gcacgacca gaccacccc acaagtata tgagggtct taaggaaaa cgtaactgc cgagaactt cgaaaaact gatcgacct gaaatggac gaatcaagc atacgcaaa gacgacctt tgtacgcaa cgacgcagt gaaatgcga agaccaggt ggtcgtcgt cgaagcggc aagcggaga ggatgtcgc agaaaggct cacgactag gtttccgga aatcgcacc agcgacggc gacgagtgt tcctacacc gggaaccgc gctatgtgt gacgaaatc caggaccat cgaactcga gaatggcga aagggtaga agacataat cgggcgctc tatactact taacatagg caaggatca tcagaatcg aaggaagaa cctaatgaa cggaatgcg caaagtagc caccctggc aagtaacaa ggggatcaa aaagtttcg gctatattc gttgatcga tgaaccgaa tagccaaaa gtttgaaac gagaaagat tactgaatg ggccctata cgacacgga gatgatggc tgaagtata tgtcccgcg agcaaggaa tagaaaatc cacagcggg agcgagtga acccgaacc agagagtga agaatggga agagcaaaa gactaaacg acgcagaac caccaaaac cataaccgt aagcaaagc tgagaccga cgagcaaac ctacgccag catgctgaa acggctcaa gggcgagat caacgtgga agagatcgg tgtcacggt caagggtgt ctacgagac gagcgacgg tagaatcaa gatcaacct gaccgaaaa agggaaggg aaactgcga gaaatttag gacggccat tgaagagaa actcggaaa caaggctga agcaaagat aaccacggc gaggaaaga actgataat aacgggcat cgacaaaga gaccacaga agaggaggt acgcctagc gatacaaca acattacac cgaaaaaac ggcatgccc agaagatac gcaggtgcg ttcgttcca ttctaacga gcgaacgaa caaacagac ggcgacggt gctagtgcc ggagaccga ggcattgca tctcctaca aaagaggaa ggtcataat cgggtggac tatgtgcag aatagtaga gaagctgag accagagag atgccatat gtgtctcaa gtacggcca ccgcgctaa agaatgtaa agagaaagc cggcgaaaa caacaccga gaagggagg cagatgctt aaagtgcgg gcgctgggg gcaccacgc aaaagcctg tcaaaacga accccactg ttacgaatg cgaacagca agggcatag ggccgattc catggcctg cccgaagta tcgaatact tgtggaaaa gatgaggag gagtacagg tgcgaataa cacctctac aaaaagacg agagtgata caacttaat actaaccgg agcagaatg gcccacgct atgctggcc aaactgaca cacgaattg gaggcgtcg gtcgttacg gtaagcgaa cctaactta acccttgcc gaaaaggaa gcatggctc gttgacgac acagggagc gcagctata aaaataact gatcggagt ttaaatatc accgacagt ggaaatggg tcgagtttt gcctgggcc aggactggc gacatgacc tacatcagc tgctacatc tcgccgaat atctcggca gcggcgttt gagacaaga ctccaagag ctggaagca ttcctcagg acccatcac ggcaacaaa ctgctcatt ggcgacttc aacgcagcg aaccgagcg tggggatcg tcga
