Status
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Gene information
General information
TC031602
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005261,TC005260
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452079.1: 0..6283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031602
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07673

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031602 RA
        MCRIVEKLR PERCHRCLK YGHRAKECK EKAGENNTE 
        KGGRCLKCG RWGHHAKAC QNEPHCYEC EQQGHRADS 
        MACPKMAHA MLAKLTHEL EASVVTVSE PNLTLAEKE 
        AWLVDDTGS AAIKITDRS LNITDSGNG SSFAWARTG 
        DMTYISCYI SPNISAAAF ETRLQELEA FLRTHHGNK 
        LLTGDFNAA NRAWGSSTN DRRGQLVIE MTEALGMTL 
        LNTGHSPTF LGSGGTSIV DLAFIDDSA AGRVRNWTV 
        LEAETMSDH RAIAIDIED HRARPATGD HRYGGKAHA 
        LRWTKDRDK QLREQLAEI LPRINTVDP ISVSRAIGE 
        AAQRSLKKI ANNRKPIFW WTDEVGNAR QRCNAARRR 
        AGRARDDRK TELWRIYKD ERKALKKEI KKAKAEAWQ 
        RLIEDIEHD PWGKGYRIA IGRAAPRQQ VSERERWRA 
        ARELFPQCA IPTYDMQEA CEPTMFSYE EIQAAAARM 
        RMGKAAGPD GLGPEIVQA AVAASPETI SGLVNDLLK 
        SQKFPEQWK RARLALIPK AKKSPDKLP KYRPICVLD 
        SLGKFYEQL ILARLLGEL EAKGGLSDS QYGFVRGRS 
        TIDAMQDVL RVVEWCSTG SYGRRELCA LILLDVANA 
        FNSVRWPDI IQALIDKGI SAYLVNLVR SYLTDRFII 
        VQHGDGFNA TCGVPQGSV LGPLLWNLV YDEVLHLDV 
        PVGVRLFAF ADDLAMVAM GMTVDELQN NANLALDTV 
        NRWMCSKHL TLAAEKTEA VIMTGRRDH ADIRFTVGD 
        SVVKPSESA RYLGVYMDR KRTFAQHIA EAAAGTTKL 
        TGKLSRILP NLRGASEGR RRTIAAAVT SKMMYGSEI 
        WSKGLDKLS NAEKVNRAQ RPIMLRVAC AYRTVATTS 
        LQVLCSMVP WDLLALERR ATFKRPENR KAEREKTIR 
        QWKDRWDKE SRHSWTKRL IPEIEPWLT RRHGLLDFY 
        TTQMMTGHG CFSSYLSRI GREATEDCW YCDCTRDDA 
        EHTFFDCPR WTEERSEVE LILGAFPEP EALVGEMLK 
        DERTKREGE RAQAPDAEA PLADGIDNS EQPTRSNVL 
        KDSPGSAAT LLMRKRGLV GESPTLSGP LRTYHQRPG 
        GRLLELPLN LGKKKKKKK VRSGQVRSG QVRSGPRVK 
        FGWSSIGRL PRWRIGESL PDMGGTGEI KYPGRTKTS 
        QDHTVTPDV VWKRGPRGF GAARKKLTV QGRDTLVPV 
        GEGVVGQAL PDGQPTTVQ TPSVRSQAE ASPKATDFK 
        RGSRQTLYE KISRSHSLG SADKKRKYT DMSPGYERS 
        IEPYKKVES PEAFVLTEA LDMVIKLGL DLERRIENN 
        TKREIKDLS ARLKRQVEI LSRTSVRAW VESHRYIEP 
        EKIQIDVDI QTEALGEGH ADELARKEE THNEEILSL 
        RSEVERLRQ ENEELRRMN RQPQEKTPH DQTTPQVYE 
        GLKENVTAE NFEKLIDLK WTNQAYAKT TFVRNDAVK 
        CEDQVVVVE AASGEDVAE RLTTRFPEI APATATSVP 
        TPGTALCVT KSRTIELEN GERVEDIIG RSILLNIGK 
        DHQNRRKNL MNGMRKVAT LASNKGIKK FRLYSLIDE 
        PNSQKFETR KITEWALYD TEMMAEVYV PRARNRKST 
        AGASEPEPE SEEWEEQKT KRRRTTKTI TVSKAETDE 
        QTYASMLKR LKGEINVEE IGVTVKGVY ETSDGRIKI 
        NLTEKGKGN CEKFRTAIE EKLGNKAEA KITTARKEL 
        IITGIDKET TEEEVRLAI QQHYTEKTA CPEDTQVRS 
        FHSNERTNK QTATVLVPE TEALHLLQK RKVIIGWTM 
        CRIVEKLRP ERCHMCLKY GHRAKECKE KAGENNTEK 
        GGRCLKCGR WGHHAKACQ NEPHCYECE QQGHRADSM 
        ACPKYRILV EKMRRSTGA NNTSTKRRE
      CDS sequence
      • >TC031602 RA
        atgtgcaga atagtagag aagctgaga ccagagaga 
        tgccatagg tgtctcaag tacggccac cgcgctaaa 
        gaatgtaaa gagaaagcc ggcgaaaac aacaccgag 
        aagggaggc agatgctta aagtgcggg cgctggggg 
        caccacgca aaagcctgt caaaacgaa ccccactgt 
        tacgaatgc gaacagcaa gggcatagg gccgattcc 
        atggcctgc ccgaagatg gcccacgct atgctggcc 
        aaactgaca cacgaattg gaggcgtcg gtcgttacg 
        gtaagcgaa cctaactta acccttgcc gaaaaggaa 
        gcatggctc gttgacgac acagggagc gcagctata 
        aaaataact gatcggagt ttaaacatc accgacagt 
        ggaaatggg tcgagtttt gcctgggcc aggactggc 
        gacatgacc tacatcagc tgctacatc tcgccgaac 
        atctcggca gcggcgttt gagacaaga ctccaagag 
        ctggaagca ttcctcagg actcatcac ggcaacaaa 
        ctgctcact ggcgacttc aacgcagcg aaccgagcg 
        tggggatcg tcgacgaat gacaggagg gggcaactc 
        gtcatcgag atgacggag gcgttggga atgaccctc 
        cttaacacc ggacactcc ccaacattt ctcggttcg 
        gggggaacg tccatagtc gacttggcg ttcatcgac 
        gatagtgcg gcaggtagg gtgaggaac tggaccgtt 
        ctcgaagcg gaaaccatg agcgaccac cgagcgatc 
        gccatcgac atcgaagac cacagggcc agacccgcg 
        acgggcgac catagatac ggaggaaag gcgcacgcg 
        ctcagatgg actaaagac cgagataag cagctccgg 
        gaacaactg gcggaaatt ctacccaga attaatacg 
        gtcgacccg atctcagtg agccgggcc attggcgag 
        gctgcccag cgctccctc aagaaaatc gctaataac 
        aggaaaccc atcttctgg tggacggac gaagttggg 
        aacgcgagg caaagatgt aacgccgca cgacgacgt 
        gcgggaagg gcgagggac gaccgcaaa accgagcta 
        tggcggatc tacaaggac gagaggaaa gcactgaag 
        aaggaaata aagaaagct aaggcagag gcctggcaa 
        aggcttata gaagacatc gaacacgac ccatggggt 
        aagggctat agaatagca attgggagg gcggcccct 
        cgacaacaa gtcagcgag cgagaaagg tggagggcg 
        gcgcgtgaa ctgtttcct cagtgcgcc attcccacg 
        tacgacatg caagaggcc tgcgaaccg acaatgttc 
        tcctacgaa gagatacag gccgcggcg gcgagaatg 
        cgcatgggc aaggcggcc ggacctgac ggcctgggc 
        cccgagata gtgcaagct gctgtagcg gcgtctccc 
        gagaccatt tctggactc gtcaacgac ttattaaaa 
        agtcaaaag ttcccggaa cagtggaag agggccaga 
        ttggcactc atacccaag gcaaagaag tcgcccgac 
        aaactaccc aaatatcgc cctatatgc gtgctggat 
        agcctgggg aagttctat gaacaacta atacttgcc 
        agactgctc ggcgagctc gaagccaag ggcgggctc 
        tccgactcc cagtacggc ttcgtgaga ggcaggtcg 
        accattgac gcgatgcag gatgtactc agggtggtc 
        gagtggtgc tccaccggc tcgtacggc agaagggag 
        ctgtgtgcc ctaatattg ctcgacgtc gccaacgca 
        ttcaatagc gtcaggtgg cccgacatt attcaggca 
        ttgattgac aaagggata agtgcgtac ctcgtcaac 
        ctggtgcgc tcgtatctc accgatcga ttcatcatc 
        gtgcagcat ggcgacgga ttcaacgcg acatgcgga 
        gtgccccag gggtcggta ctgggcccg ctcctctgg 
        aatctcgtt tacgacgag gtgctacat ctcgacgtt 
        cccgttggc gtgcgcctt ttcgccttc gccgacgac 
        ctggcgatg gtcgccatg gggatgacg gttgacgag 
        ttacaaaat aatgcgaac cttgccctc gacactgtg 
        aaccgatgg atgtgtagc aaacactta acgctagcg 
        gcggagaag accgaggcc gttataatg acgggcagg 
        agagaccac gctgacatc aggttcact gtcggcgac 
        agtgtggta aaacccagc gagagcgcc agatacctg 
        ggggtatac atggacagg aaaagaacg ttcgcccaa 
        cacatagcg gaagccgct gcggggacc accaagttg 
        acgggcaag ctatcaagg attctcccg aatctccgg 
        ggagcctcg gaggggaga cggaggacg attgcagct 
        gcggtcacc agcaaaatg atgtacggg tccgagata 
        tggtccaaa gggctggac aaattaagc aacgccgag 
        aaagtaaac agggctcag agaccaata atgttaagg 
        gttgcgtgc gcctaccgc actgtggcg accacctct 
        ctccaagta ctgtgttcc atggtgccg tgggacttg 
        ctggcactg gagaggagg gcgaccttc aagagaccc 
        gaaaacagg aaagcggaa agggaaaaa actattcga 
        cagtggaag gataggtgg gacaaggag agccggcac 
        agctggacc aagaggctt attcccgag attgagccc 
        tggctgacg aggaggcac ggcctgcta gacttttac 
        accacacag atgatgacc ggccacggc tgcttttcc 
        tcctacctg tctaggata gggagggag gcaaccgag 
        gactgctgg tactgcgac tgcacgaga gacgacgct 
        gaacataca ttttttgat tgcccaagg tggacggag 
        gaaaggagc gaagtcgaa ctaatattg ggggcattc 
        cccgaaccg gaagcactc gtaggggaa atgctaaag 
        gatgagagg acgaagaga gaaggagaa agggcgcag 
        gcgccagac gctgaggct cccctagca gatggcata 
        gacaacagc gaacagccg acccgaagt aatgtcttg 
        aaggatagt cccgggtcg gctgccaca ttgctgatg 
        aggaaaagg ggtttagtg ggtgagagc cccacactc 
        tccgggccg ctaagaacg taccaccaa aggcccggg 
        ggtcggctg ttagagctt cccctcaac ctcggtaaa 
        aaaaaaaaa aaaaaaaag gtcaggtca ggtcaggtc 
        aggtcaggt caggtcagg tcaggtccc cgtgtgaag 
        tttggctgg tcttccatc gggcgcctc cccaggtgg 
        cggataggg gaaagcctc ccagatatg ggtggtacc 
        ggggaaatc aaatacccg gggcggacc aaaaccagc 
        caagatcac accgtaact ccagacgtg gtctggaaa 
        cgggggccg cgcggtttc ggggctgcg aggaagaaa 
        cttacggtg caagggagg gatacccta gtacccgtc 
        ggtgaggga gtggttgga caggctctc cctgacggt 
        cagccaacg actgttcag acaccttcg gtgaggagt 
        caggccgag gcgtcaccg aaagctact gacttcaaa 
        cgaggaagc aggcaaaca ctgtacgag aaaatatca 
        agaagccac tcgctagga agtgccgat aagaaacgg 
        aagtacacg gatatgtct ccaggctac gaacggagc 
        atagaaccc tacaaaaag gttgagagt ccagaggcc 
        tttgtgcta acagaagca ctggacatg gtaattaaa 
        ctaggtttg gatcttgaa aggagaatt gaaaacaac 
        acgaaaagg gaaatcaag gacctatcg gcaagactc 
        aaaaggcag gtggagatt cttagtcga acttctgtg 
        agggcgtgg gtcgaatca cacagatac attgagccc 
        gaaaaaata caaattgat gtcgacata cagaccgag 
        gcgttaggc gaagggcac gctgatgaa ctagcccgg 
        aaagaagaa acacacaac gaagaaatt ctctcactg 
        cggtccgaa gtagagaga ctgagacaa gagaacgaa 
        gagctgaga agaatgaac agacaacca caggagaaa 
        acaccgcac gaccagacc accccacaa gtatatgag 
        ggtcttaag gaaaacgta actgccgag aacttcgaa 
        aaactgatc gacctgaaa tggacgaat caagcatac 
        gcaaagacg acctttgta cgcaacgac gcagtgaaa 
        tgcgaagac caggtggtc gtcgtcgaa gcggcaagc 
        ggagaggat gtcgcagaa aggctcacg actaggttt 
        ccggaaatc gcaccagcg acggcgacg agtgttcct 
        acaccggga accgcgcta tgtgtgacg aaatccagg 
        accatcgaa ctcgagaat ggcgaaagg gtagaagac 
        ataatcggg cgctctata ctacttaac ataggcaag 
        gatcatcag aatcgaagg aagaaccta atgaacgga 
        atgcgcaaa gtagccacc ctggcaagt aacaagggg 
        atcaaaaag tttcggcta tattcgttg atcgatgaa 
        ccgaatagc caaaagttt gaaacgaga aagattact 
        gaatgggcc ctatacgac acggagatg atggctgaa 
        gtatatgtc ccgcgagca aggaataga aaatccaca 
        gcgggagcg agtgaaccc gaaccagag agtgaagaa 
        tgggaagag caaaagact aaacgacgc agaaccacc 
        aaaaccata accgtaagc aaagctgag accgacgag 
        caaacctac gccagcatg ctgaaacgg ctcaagggc 
        gagatcaac gtggaagag atcggtgtc acggtcaag 
        ggtgtctac gagacgagc gacggtaga atcaagatc 
        aacctgacc gaaaaaggg aagggaaac tgcgagaaa 
        tttaggacg gccattgaa gagaaactc ggaaacaag 
        gctgaagca aagataacc acggcgagg aaagaactg 
        ataataacg ggcatcgac aaagagacc acagaagag 
        gaggtacgc ctagcgata caacaacat tacaccgaa 
        aaaacggca tgcccagaa gatacgcag gtgcgttcg 
        ttccattct aacgagcga acgaacaaa cagacggcg 
        acggtgcta gtgccggag accgaggca ttgcatctc 
        ctacaaaag aggaaggtc ataatcggg tggactatg 
        tgcagaata gtagagaag ctgagacca gagagatgc 
        catatgtgt ctcaagtac ggccaccgc gctaaagaa 
        tgtaaagag aaagccggc gaaaacaac accgagaag 
        ggaggcaga tgcttaaag tgcgggcgc tgggggcac 
        cacgcaaaa gcctgtcaa aacgaaccc cactgttac 
        gaatgcgaa cagcaaggg catagggcc gattccatg 
        gcctgcccg aagtatcga atacttgtg gaaaagatg 
        aggaggagt acaggtgcg aataacacc tctacaaaa 
        agacgagag tga
      mRNA sequence
      • >TC031602 RA
        cgagcgaac gaacaaaca gacggcgac ggtgctagt 
        gccggagac cgaggcatt gcatctcct acaaaagag 
        gaaggtcat aatcgggtg gactatgtg cagaatagt 
        agagaagct gagaccaga gagatgcca taggtgtct 
        caagtacgg ccaccgcgc taaagaatg taaagagaa 
        agccggcga aaacaacac cgagaaggg aggcagatg 
        cttaaagtg cgggcgctg ggggcacca cgcaaaagc 
        ctgtcaaaa cgaacccca ctgttacga atgcgaaca 
        gcaagggca tagggccga ttccatggc ctgcccgaa 
        gatggccca cgctatgct ggccaaact gacacacga 
        attggaggc gtcggtcgt tacggtaag cgaacctaa 
        cttaaccct tgccgaaaa ggaagcatg gctcgttga 
        cgacacagg gagcgcagc tataaaaat aactgatcg 
        gagtttaaa catcaccga cagtggaaa tgggtcgag 
        ttttgcctg ggccaggac tggcgacat gacctacat 
        cagctgcta catctcgcc gaacatctc ggcagcggc 
        gtttgagac aagactcca agagctgga agcattcct 
        caggactca tcacggcaa caaactgct cactggcga 
        cttcaacgc agcgaaccg agcgtgggg atcgtcgac 
        gaatgacag gagggggca actcgtcat cgagatgac 
        ggaggcgtt gggaatgac cctccttaa caccggaca 
        ctccccaac atttctcgg ttcgggggg aacgtccat 
        agtcgactt ggcgttcat cgacgatag tgcggcagg 
        tagggtgag gaactggac cgttctcga agcggaaac 
        catgagcga ccaccgagc gatcgccat cgacatcga 
        agaccacag ggccagacc cgcgacggg cgaccatag 
        atacggagg aaaggcgca cgcgctcag atggactaa 
        agaccgaga taagcagct ccgggaaca actggcgga 
        aattctacc cagaattaa tacggtcga cccgatctc 
        agtgagccg ggccattgg cgaggctgc ccagcgctc 
        cctcaagaa aatcgctaa taacaggaa acccatctt 
        ctggtggac ggacgaagt tgggaacgc gaggcaaag 
        atgtaacgc cgcacgacg acgtgcggg aagggcgag 
        ggacgaccg caaaaccga gctatggcg gatctacaa 
        ggacgagag gaaagcact gaagaagga aataaagaa 
        agctaaggc agaggcctg gcaaaggct tatagaaga 
        catcgaaca cgacccatg gggtaaggg ctatagaat 
        agcaattgg gagggcggc ccctcgaca acaagtcag 
        cgagcgaga aaggtggag ggcggcgcg tgaactgtt 
        tcctcagtg cgccattcc cacgtacga catgcaaga 
        ggcctgcga accgacaat gttctccta cgaagagat 
        acaggccgc ggcggcgag aatgcgcat gggcaaggc 
        ggccggacc tgacggcct gggccccga gatagtgca 
        agctgctgt agcggcgtc tcccgagac catttctgg 
        actcgtcaa cgacttatt aaaaagtca aaagttccc 
        ggaacagtg gaagagggc cagattggc actcatacc 
        caaggcaaa gaagtcgcc cgacaaact acccaaata 
        tcgccctat atgcgtgct ggatagcct ggggaagtt 
        ctatgaaca actaatact tgccagact gctcggcga 
        gctcgaagc caagggcgg gctctccga ctcccagta 
        cggcttcgt gagaggcag gtcgaccat tgacgcgat 
        gcaggatgt actcagggt ggtcgagtg gtgctccac 
        cggctcgta cggcagaag ggagctgtg tgccctaat 
        attgctcga cgtcgccaa cgcattcaa tagcgtcag 
        gtggcccga cattattca ggcattgat tgacaaagg 
        gataagtgc gtacctcgt caacctggt gcgctcgta 
        tctcaccga tcgattcat catcgtgca gcatggcga 
        cggattcaa cgcgacatg cggagtgcc ccaggggtc 
        ggtactggg cccgctcct ctggaatct cgtttacga 
        cgaggtgct acatctcga cgttcccgt tggcgtgcg 
        ccttttcgc cttcgccga cgacctggc gatggtcgc 
        catggggat gacggttga cgagttaca aaataatgc 
        gaaccttgc cctcgacac tgtgaaccg atggatgtg 
        tagcaaaca cttaacgct agcggcgga gaagaccga 
        ggccgttat aatgacggg caggagaga ccacgctga 
        catcaggtt cactgtcgg cgacagtgt ggtaaaacc 
        cagcgagag cgccagata cctgggggt atacatgga 
        caggaaaag aacgttcgc ccaacacat agcggaagc 
        cgctgcggg gaccaccaa gttgacggg caagctatc 
        aaggattct cccgaatct ccggggagc ctcggaggg 
        gagacggag gacgattgc agctgcggt caccagcaa 
        aatgatgta cgggtccga gatatggtc caaagggct 
        ggacaaatt aagcaacgc cgagaaagt aaacagggc 
        tcagagacc aataatgtt aagggttgc gtgcgccta 
        ccgcactgt ggcgaccac ctctctcca agtactgtg 
        ttccatggt gccgtggga cttgctggc actggagag 
        gagggcgac cttcaagag acccgaaaa caggaaagc 
        ggaaaggga aaaaactat tcgacagtg gaaggatag 
        gtgggacaa ggagagccg gcacagctg gaccaagag 
        gcttattcc cgagattga gccctggct gacgaggag 
        gcacggcct gctagactt ttacaccac acagatgat 
        gaccggcca cggctgctt ttcctccta cctgtctag 
        gatagggag ggaggcaac cgaggactg ctggtactg 
        cgactgcac gagagacga cgctgaaca tacattttt 
        tgattgccc aaggtggac ggaggaaag gagcgaagt 
        cgaactaat attgggggc attccccga accggaagc 
        actcgtagg ggaaatgct aaaggatga gaggacgaa 
        gagagaagg agaaagggc gcaggcgcc agacgctga 
        ggctcccct agcagatgg catagacaa cagcgaaca 
        gccgacccg aagtaatgt cttgaagga tagtcccgg 
        gtcggctgc cacattgct gatgaggaa aaggggttt 
        agtgggtga gagccccac actctccgg gccgctaag 
        aacgtacca ccaaaggcc cgggggtcg gctgttaga 
        gcttcccct caacctcgg taaaaaaaa aaaaaaaaa 
        aaaggtcag gtcaggtca ggtcaggtc aggtcaggt 
        caggtcagg tccccgtgt gaagtttgg ctggtcttc 
        catcgggcg cctccccag gtggcggat aggggaaag 
        cctcccaga tatgggtgg taccgggga aatcaaata 
        cccggggcg gaccaaaac cagccaaga tcacaccgt 
        aactccaga cgtggtctg gaaacgggg gccgcgcgg 
        tttcggggc tgcgaggaa gaaacttac ggtgcaagg 
        gagggatac cctagtacc cgtcggtga gggagtggt 
        tggacaggc tctccctga cggtcagcc aacgactgt 
        tcagacacc ttcggtgag gagtcaggc cgaggcgtc 
        accgaaagc tactgactt caaacgagg aagcaggca 
        aacactgta cgagaaaat atcaagaag ccactcgct 
        aggaagtgc cgataagaa acggaagta cacggatat 
        gtctccagg ctacgaacg gagcataga accctacaa 
        aaaggttga gagtccaga ggcctttgt gctaacaga 
        agcactgga catggtaat taaactagg tttggatct 
        tgaaaggag aattgaaaa caacacgaa aagggaaat 
        caaggacct atcggcaag actcaaaag gcaggtgga 
        gattcttag tcgaacttc tgtgagggc gtgggtcga 
        atcacacag atacattga gcccgaaaa aatacaaat 
        tgatgtcga catacagac cgaggcgtt aggcgaagg 
        gcacgctga tgaactagc ccggaaaga agaaacaca 
        caacgaaga aattctctc actgcggtc cgaagtaga 
        gagactgag acaagagaa cgaagagct gagaagaat 
        gaacagaca accacagga gaaaacacc gcacgacca 
        gaccacccc acaagtata tgagggtct taaggaaaa 
        cgtaactgc cgagaactt cgaaaaact gatcgacct 
        gaaatggac gaatcaagc atacgcaaa gacgacctt 
        tgtacgcaa cgacgcagt gaaatgcga agaccaggt 
        ggtcgtcgt cgaagcggc aagcggaga ggatgtcgc 
        agaaaggct cacgactag gtttccgga aatcgcacc 
        agcgacggc gacgagtgt tcctacacc gggaaccgc 
        gctatgtgt gacgaaatc caggaccat cgaactcga 
        gaatggcga aagggtaga agacataat cgggcgctc 
        tatactact taacatagg caaggatca tcagaatcg 
        aaggaagaa cctaatgaa cggaatgcg caaagtagc 
        caccctggc aagtaacaa ggggatcaa aaagtttcg 
        gctatattc gttgatcga tgaaccgaa tagccaaaa 
        gtttgaaac gagaaagat tactgaatg ggccctata 
        cgacacgga gatgatggc tgaagtata tgtcccgcg 
        agcaaggaa tagaaaatc cacagcggg agcgagtga 
        acccgaacc agagagtga agaatggga agagcaaaa 
        gactaaacg acgcagaac caccaaaac cataaccgt 
        aagcaaagc tgagaccga cgagcaaac ctacgccag 
        catgctgaa acggctcaa gggcgagat caacgtgga 
        agagatcgg tgtcacggt caagggtgt ctacgagac 
        gagcgacgg tagaatcaa gatcaacct gaccgaaaa 
        agggaaggg aaactgcga gaaatttag gacggccat 
        tgaagagaa actcggaaa caaggctga agcaaagat 
        aaccacggc gaggaaaga actgataat aacgggcat 
        cgacaaaga gaccacaga agaggaggt acgcctagc 
        gatacaaca acattacac cgaaaaaac ggcatgccc 
        agaagatac gcaggtgcg ttcgttcca ttctaacga 
        gcgaacgaa caaacagac ggcgacggt gctagtgcc 
        ggagaccga ggcattgca tctcctaca aaagaggaa 
        ggtcataat cgggtggac tatgtgcag aatagtaga 
        gaagctgag accagagag atgccatat gtgtctcaa 
        gtacggcca ccgcgctaa agaatgtaa agagaaagc 
        cggcgaaaa caacaccga gaagggagg cagatgctt 
        aaagtgcgg gcgctgggg gcaccacgc aaaagcctg 
        tcaaaacga accccactg ttacgaatg cgaacagca 
        agggcatag ggccgattc catggcctg cccgaagta 
        tcgaatact tgtggaaaa gatgaggag gagtacagg 
        tgcgaataa cacctctac aaaaagacg agagtgata 
        caacttaat actaaccgg agcagaatg gcccacgct 
        atgctggcc aaactgaca cacgaattg gaggcgtcg 
        gtcgttacg gtaagcgaa cctaactta acccttgcc 
        gaaaaggaa gcatggctc gttgacgac acagggagc 
        gcagctata aaaataact gatcggagt ttaaatatc 
        accgacagt ggaaatggg tcgagtttt gcctgggcc 
        aggactggc gacatgacc tacatcagc tgctacatc 
        tcgccgaat atctcggca gcggcgttt gagacaaga 
        ctccaagag ctggaagca ttcctcagg acccatcac 
        ggcaacaaa ctgctcatt ggcgacttc aacgcagcg 
        aaccgagcg tggggatcg tcga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY