Status
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Gene information
General information
TC011883 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12258309..12265742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011883
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_07668

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011883 RA
        MYSGGRASS EMGLPGCKW FVISVCCMT 
        GIYGYSIRP IEDENGQPI NLDRDFLFT 
        YDTRQFLDS FEDREKEMV DGSKFEATK 
        PPGDSTSTP LDSWSVVWY IASFGGLIV 
        FFLIVSCSE WCCRRALRN SQSCGRSAP 
        AAAAPSVPD TPPPSYDQF APPSYESIC 
        LGRNGRGGE KSEYDIYVV PVHAFGTIM 
        ESRQEDAPP SYFSASGRS FVPAVTESN 
        QVSRVT
      CDS sequence
      • >TC011883 RA
        atgtatagt ggcggacga gcgtccagc gaaatgggt 
        cttcccggc tgcaaatgg ttcgttata agtgtgtgc 
        tgtatgacg gggatttat ggctacagc atacggccg 
        atagaagac gagaatgga caacccatc aatctagac 
        agggatttt ttgttcacc tatgacacc agacaattc 
        ctagacagt ttcgaagac agagaaaag gaaatggtc 
        gacggttcc aaattcgaa gccaccaaa cctccaggc 
        gacagcacg tccacgcct ctggactcg tggtcggtg 
        gtgtggtac atcgcctcc ttcgggggc ctgatcgtg 
        ttcttcctc atcgtgtcg tgttcggag tggtgttgc 
        cggcgggcg ctccgcaac agccagagc tgcggcagg 
        agcgcgccc gccgccgcc gcccccagc gtgcccgac 
        acgccgccc ccgtcctac gaccagttc gctccgccc 
        tcgtacgag agtatctgt ctggggcgg aacggcagg 
        ggcggcgag aagagcgag tacgacatc tacgtggtt 
        cctgtccac gcttttggt actatcatg gaaagcaga 
        caggaagat gcgcccccg tcttacttc agcgcctcc 
        ggtcgcagc tttgttcca gctgtgacg gaaagtaat 
        caagtgtcg cgagtgacg tga
      mRNA sequence
      • >TC011883 RA
        actaaaaat agaacacaa tgggatttc agacccaat 
        ccaatgcca gaaccagtt cttgatttg aacagtttg 
        tttgctcaa acgaccatg tcattccta atattactg 
        cgccaaatg aaaaactta aatactgaa accgtacta 
        aatggttgt gacgaattt ctacatttc gcaagtttt 
        atgggtgat tgaagatgg cacaggacg aaaaaactc 
        aatgtcatg gtgcggtag tgggtattt cattgttcg 
        ttccgaaaa tttgtgtat taatgcttt acggacgtc 
        actgtcgat agtaaagca aatatccaa agcaattaa 
        tgtaaaaaa gatggagat aagcttgac gtcacattg 
        cgatattat aattggccg caggcgagt tctgcccag 
        tacaaaggt gaggtaatt ccgtgattc atttttgtg 
        acgtcctct aacaaaaat tgcagatca gatcgccaa 
        tccaccaat ctttggcga tttttgtaa gcacttgca 
        tttgaccca gtttgcgca attcggtta atatgtcgc 
        tggggcaca tgtatttgg aaaatgact cgacacttc 
        aaatattta cgatcaaaa aattcctca gcgctggac 
        ttccacccc acactttga aatacccaa attattgct 
        aataaaatg tttcagaga taaatgcca tcgtgttaa 
        ttgagataa aaatattaa taaccctag atatctatt 
        tttagtctc ccatagccc atagaaacc atcaaggct 
        atctatcga cgccagaaa actatagga aattcattt 
        ccatttgat gaaaacaac aacacttta aaaaactgt 
        ttgtccaat taattacaa gtgttattt tcgtgctgc 
        ttgcagttt gtttgaaca atttgcgtg aagctcgtc 
        ccataaaga taaacaaat gattgcgat acaacgcag 
        gtcaagaaa ccttgccta atgttaatt cttttacgt 
        acatattaa tggagcaca ccacaaagg aaatcaaag 
        cagcattat gcattgacc ttgtactgc caaaagcga 
        aataatcaa ttttcgcgc aaatttccc tcaaaaccg 
        aagaaaacc acgccgcgt cctattaat aaccgggaa 
        aaatgcgcc gcgctcatt cgctcctgc accagatgg 
        acagatcgg cacttttcc ggagaaaaa cagcgggtc 
        caccggtcg aaaagggca ctcgaccga taagccggt 
        ccagcgaag aagttcgat gacgaaacg cctcgcatg 
        gaaaccgtc aagatcatt atcacctgc tgataagcc 
        acgacaggt acaaaaccg accggatcg cgacgtaaa 
        ctccattcc tgaaatcac acacggacg gtggacctc 
        gcgctttgc ggtaattaa taaaaagtg tgcggtgtt 
        gtgtcgatg tatagtggc ggacgagcg tccagcgaa 
        atgggtctt cccggctgc aaatggttc gttataagt 
        gtgtgctgt atgacgggg atttatggc tacagcata 
        cggccgata gaagacgag aatggacaa cccatcaat 
        ctagacagg gattttttg ttcacctat gacaccaga 
        caattccta gacagtttc gaagacaga gaaaaggaa 
        atggtcgac ggttccaaa ttcgaagcc accaaacct 
        ccaggcgac agcacgtcc acgcctctg gactcgtgg 
        tcggtggtg tggtacatc gcctccttc gggggcctg 
        atcgtgttc ttcctcatc gtgtcgtgt tcggagtgg 
        tgttgccgg cgggcgctc cgcaacagc cagagctgc 
        ggcaggagc gcgcccgcc gccgccgcc cccagcgtg 
        cccgacacg ccgcccccg tcctacgac cagttcgct 
        ccgccctcg tacgagagt atctgtctg gggcggaac 
        ggcaggggc ggcgagaag agcgagtac gacatctac 
        gtggttcct gtccacgct tttggtact atcatggaa 
        agcagacag gaagatgcg cccccgtct tacttcagc 
        gcctccggt cgcagcttt gttccagct gtgacggaa 
        agtaatcaa gtgtcgcga gtgacgtga aaaacgagc 
        gagtttatt gcttgataa ggaatctga aggcaataa 
        tgaatgatt gttccggtt gagaaacgt accaaatat 
        gagattatt gtagtgttg gggcgccga tcacgttgt 
        gatattcta gtgatagga ggaagtatt acggacttg 
        atgagccat gagattctc agggtgtag agttgactc 
        gaaacagta ttactttat tacccaaaa cttgtgcct 
        tatttattt aagtctact taagcataa gactgtcgg 
        agtagatta agttttgta cctgcctgt agataattt 
        atttatacg tctcttgta ttcctcttg tgatattgt 
        ttgtgtaaa gtgacgcca gttgaagca ctgaccact 
        gtattgttc tagactagg tgataaata aaacgaaaa 
        caatctaag caaacattt taattatta cggttatga 
        cgaaaaatc ttccatgcc caactgtta atgcaattt 
        aaatttatt gtgcattcc aatcgcaca ttagactca 
        aggtaaggc tggagtacg agtcgtttg tgcaaattt 
        gtacaagtt ctatatttg tgttcagac aaagataag 
        ttcggtcct gtgagaaaa ataaaactg ttaattaaa 
        ttagcgact tatctcgta atgacgtcc ccattccaa 
        ctaataaat caaatcttg ttccggatt tactgattt 
        attgccgat caaaagctg aggaaaata ccgatgtgg 
        acaatttta cacactctt gacattgaa ttacggaac 
        gttgagtaa tcaagcgag aaaacaaaa ttacataaa 
        attcgacac aatctacct actcattga taccccaca 
        atgatgcaa gatatgatt tcacgtcga gtgtttgtc 
        acgtttaca acattttat tactccgta aacgtccac 
        aacaaataa cacagtcat tattccgac tggctattg 
        gtctataaa gataacacg gaataaaaa gattacaac 
        caacaaaga aaaaataaa acaaactag gtcgcaaaa 
        taaaagaat gcgccaaac atttccaaa caaagtagc 
        gataaaaat taaaatgat tagccctga gctgggggt 
        caattttgt gattggaca ataaaatta ataggtatg 
        tacgtgaca actaaaatg ggaacttaa tgtttgcgt 
        ttttaattg aaaaataac tgaacattt tgacttatc 
        gcagttgat ggcctcgga acaagcacc aaagaattt 
        ttattttat aagtgtttt cgaagtaaa aaaagagta 
        ataattatt acacaaaaa tattttggg cgccaggct 
        atcaactgt gataaaaag tgtaaaaaa ctgatttgt 
        tagttgcta tctctaaag aaaaatata aacaatttt 
        tcacgtcag gtgctaagt gcaatactt aaaacaata 
        acgcgaagc cttggacct gtagtcgta aaaatattg 
        tcggtattg taaaataaa atttacatt tatta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY