Status
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Gene information
General information
TC012601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11965374..11969178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012601
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber complete not present - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012601 RA
      MIYDPKWGS GGKIIASLV GHTKRVNSV RWLSGAELV 
      SGACDHTAI VWKLTGGKY APMVLQHES IVNIVDGIC 
      KNEGVVVVT VAMDAVVKI WFRAIESDD FTLIRDIKG 
      GHSICVGVR IAFFPQKES LILACAMDN SAIDLYVEE 
      NEFAHCHTL KGHEDWVRG LDFTTDGKD LLLASASQD 
      CYIRLWRFA PQSEISEPH NVIKLKNAL YKVSLDTIL 
      TGHEGWIYS VHWSPKSPQ LLSSSLDKS MIIWEFDSS 
      TNLWTEKVR VGEVGGNTL GFYGGVFSP DGHNILAHG 
      YNGAFHLWE NASSGWKPC VTIGGHFAE VTDLAWDPQ 
      GEFLLTVSA DQTTRIHAP WSDDKDATW HEIARPQVH 
      GYDLSSVAV LSRYRFASS AEEKVIRTF EAPRNFVEN 
      FARICKIND VVEGSAPKG AAVPSLGLS NKAVFLSEG 
      GGDEESHFT EVVLTAPPT EETLLQNTL WPEVQKLYG 
      HGYEVYSLA SSPDGRFLA SACKATTPE HAAILLWDT 
      SNWKQIQKL VSHTLTVVQ LSFSPDSQH LLSVSRDRR 
      WSLFSKNPN ATFELVATT DKRTSIHTR IIWCCGWTH 
      DSRYFATGS RDGKVAVWT KNANKQETG VLGQYEAAS 
      VHLELKNES VTAVAFAPD CVFGSYLIA VGLEVGVIH 
      CLKWSTSAW ERILFLDKN AAHHLTVKR LAFRPAFGA 
      AGQEKDDRV LQLASCGSD NTVVVHNFL IK
    CDS sequence
    • >TC012601 RA
      atgatttat gacccaaaa tggggctcc ggtggtaaa 
      attatagcg agtctggtg gggcatacg aagcgcgtt 
      aactcagtc aggtggctc tcaggggcg gagctggtg 
      tcaggggcg tgtgaccac actgcgatt gtttggaag 
      ttaactggc ggcaaatat gcccccatg gtgctacag 
      cacgaaagc atcgtaaat atagttgac gggatttgt 
      aaaaatgaa ggggtggtt gttgtcaca gttgcgatg 
      gacgctgtc gtaaagata tggttcagg gcaatcgaa 
      tcagacgat tttacgcta attagagac ataaagggt 
      gggcactcg atttgtgta ggggtcaga attgcgttt 
      ttcccccaa aaagaatct ttaatcctg gcgtgtgct 
      atggataat tctgcaatt gatttgtat gttgaggaa 
      aatgaattc gcacattgt cacaccttg aagggtcat 
      gaggactgg gtcagggga ctcgatttt acaactgat 
      ggtaaagac ttgctcctg gcgtctgct tcgcaggac 
      tgttatatc cgtctgtgg cgctttgcc cctcagtcc 
      gaaatctcg gaaccccac aatgtaatc aaattaaaa 
      aacgccttg tataaagtg tctctagac acaattttg 
      actggccat gaaggctgg atttactcc gtgcactgg 
      agccccaaa tcgccccaa ctactctca tcctccctc 
      gacaaatcc atgataatc tgggagttc gactcttcc 
      actaaccta tggaccgag aaagtaagg gttggggaa 
      gtcgggggc aacactttg gggttctac ggcggagtc 
      ttcagcccc gacggccac aatatcctc gcccatggc 
      tacaacggc gccttccac ttgtgggaa aacgcctcc 
      agcgggtgg aaaccgtgc gttaccatc ggggggcac 
      ttcgccgaa gtcacagac ttggcgtgg gacccccag 
      ggggagttc ctcctgacg gtctccgcc gaccaaacg 
      acgcgaatc cacgccccc tggagcgac gataaagac 
      gccacgtgg cacgaaatc gcgaggccc caagtgcac 
      ggttatgat ttgagttca gtggcggtt ttgtcgcgg 
      tataggttt gcctcctcg gctgaggaa aaagtgatt 
      cggacgttt gaggctccg aggaatttt gtcgaaaat 
      tttgcgcga atttgtaaa attaacgat gttgtggag 
      ggaagtgca cccaaaggg gcggcggtc ccctcgttg 
      gggctctcg aacaaagcg gtgtttttg agcgaggga 
      gggggcgat gaagagtcg catttcacg gaagtggtc 
      ctcacagct cctcccacc gaggagacg cttctccag 
      aacactttg tggccggag gtgcagaag ctctacggt 
      cacggttac gaggtctac tctttggcg tcgtcccca 
      gacggtcgt tttttggct tcggcttgt aaagcgaca 
      acccccgaa cacgccgcg atcctactt tgggacacc 
      tccaactgg aaacaaatc caaaagctt gtctctcat 
      acattaacc gtagtccag ttgtctttc tctcccgac 
      tcccaacac ctcctttcg gtgtcgcgt gaccgccga 
      tggtccctc ttttcgaaa aacccaaac gccactttc 
      gagctggtg gccacaaca gacaagaga acatcaatc 
      cacactagg ataatctgg tgctgcggg tggacgcac 
      gactcgaga tatttcgcg acggggtca cgcgacggc 
      aaagtggcc gtttggacg aagaacgca aacaagcaa 
      gaaacgggt gttttaggg cagtatgaa gccgcctca 
      gtgcacttg gagctgaaa aatgagtca gtaacggcg 
      gtcgctttt gcaccggac tgcgttttc gggagttat 
      ttgattgcc gtcgggtta gaagtcggg gttattcac 
      tgtttgaaa tggtcaaca agcgcctgg gagagaatt 
      ttattcttg gataaaaat gctgctcat cacttgacc 
      gtgaagaga ttagccttc cggcccgct ttcggcgca 
      gcgggccag gaaaaggac gacagggtt ttacaactg 
      gccagttgc ggcagcgac aacaccgtt gttgtacat 
      aattttttg ataaaataa
    mRNA sequence
    • >TC012601 RA
      gaacacgag aggtgttac gtgtcttgt aactgcaac 
      cagaccccg actgcggca cattggggt gccaataac 
      ctaatctgt tacgccgcc tgtaactcc atcatgatt 
      tatgaccca aaatggggc tccggtggt aaaattata 
      gcgagtctg gtggggcat acgaagcgc gttaactca 
      gtcaggtgg ctctcaggg gcggagctg gtgtcaggg 
      gcgtgtgac cacactgcg attgtttgg aagttaact 
      ggcggcaaa tatgccccc atggtgcta cagcacgaa 
      agcatcgta aatatagtt gacgggatt tgtaaaaat 
      gaaggggtg gttgttgtc acagttgcg atggacgct 
      gtcgtaaag atatggttc agggcaatc gaatcagac 
      gattttacg ctaattaga gacataaag ggtgggcac 
      tcgatttgt gtaggggtc agaattgcg tttttcccc 
      caaaaagaa tctttaatc ctggcgtgt gctatggat 
      aattctgca attgatttg tatgttgag gaaaatgaa 
      ttcgcacat tgtcacacc ttgaagggt catgaggac 
      tgggtcagg ggactcgat tttacaact gatggtaaa 
      gacttgctc ctggcgtct gcttcgcag gactgttat 
      atccgtctg tggcgcttt gcccctcag tccgaaatc 
      tcggaaccc cacaatgta atcaaatta aaaaacgcc 
      ttgtataaa gtgtctcta gacacaatt ttgactggc 
      catgaaggc tggatttac tccgtgcac tggagcccc 
      aaatcgccc caactactc tcatcctcc ctcgacaaa 
      tccatgata atctgggag ttcgactct tccactaac 
      ctatggacc gagaaagta agggttggg gaagtcggg 
      ggcaacact ttggggttc tacggcgga gtcttcagc 
      cccgacggc cacaatatc ctcgcccat ggctacaac 
      ggcgccttc cacttgtgg gaaaacgcc tccagcggg 
      tggaaaccg tgcgttacc atcgggggg cacttcgcc 
      gaagtcaca gacttggcg tgggacccc cagggggag 
      ttcctcctg acggtctcc gccgaccaa acgacgcga 
      atccacgcc ccctggagc gacgataaa gacgccacg 
      tggcacgaa atcgcgagg ccccaagtg cacggttat 
      gatttgagt tcagtggcg gttttgtcg cggtatagg 
      tttgcctcc tcggctgag gaaaaagtg attcggacg 
      tttgaggct ccgaggaat tttgtcgaa aattttgcg 
      cgaatttgt aaaattaac gatgttgtg gagggaagt 
      gcacccaaa ggggcggcg gtcccctcg ttggggctc 
      tcgaacaaa gcggtgttt ttgagcgag ggagggggc 
      gatgaagag tcgcatttc acggaagtg gtcctcaca 
      gctcctccc accgaggag acgcttctc cagaacact 
      ttgtggccg gaggtgcag aagctctac ggtcacggt 
      tacgaggtc tactctttg gcgtcgtcc ccagacggt 
      cgttttttg gcttcggct tgtaaagcg acaaccccc 
      gaacacgcc gcgatccta ctttgggac acctccaac 
      tggaaacaa atccaaaag cttgtctct catacatta 
      accgtagtc cagttgtct ttctctccc gactcccaa 
      cacctcctt tcggtgtcg cgtgaccgc cgatggtcc 
      ctcttttcg aaaaaccca aacgccact ttcgagctg 
      gtggccaca acagacaag agaacatca atccacact 
      aggataatc tggtgctgc gggtggacg cacgactcg 
      agatatttc gcgacgggg tcacgcgac ggcaaagtg 
      gccgtttgg acgaagaac gcaaacaag caagaaacg 
      ggtgtttta gggcagtat gaagccgcc tcagtgcac 
      ttggagctg aaaaatgag tcagtaacg gcggtcgct 
      tttgcaccg gactgcgtt ttcgggagt tatttgatt 
      gccgtcggg ttagaagtc ggggttatt cactgtttg 
      aaatggtca acaagcgcc tgggagaga attttattc 
      ttggataaa aatgctgct catcacttg accgtgaag 
      agattagcc ttccggccc gctttcggc gcagcgggc 
      caggaaaag gacgacagg gttttacaa ctggccagt 
      tgcggcagc gacaacacc gttgttgta cataatttt 
      ttgataaaa taaaatttt cgaacaatg ctttgtttc 
      atttggggc gtgtcaggg gtggcatta tgaagccgg 
      cggaaataa aattctaaa gctgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY