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Gene information
General information
TC012579 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11660098..11661966
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012579
    iBeetle screen iB_07657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012579 RA
      MLPEQKDLI YKHFPQLVQ QCDLEQIVE IMIKKGNLN 
      QDFLDKLPL ESDKAHNNR QILFKLLGL EEVNTFSGL 
      VDGLRKTGC ARLANILEN KLETIIESN ILHPLDVNI 
      DVKFSTQFY DTERYNRTG FYSTRSKNR GYVLIINNI 
      NFAQSYDER HGAEADERN LRQLFEKMG LTVFCYRDL 
      NAYDMYFKT REFSEKLPV TQPDIGIVI IMSHGLVIN 
      NETMIVSSD NKYLAEESF KKIFNNENC ALFRRKPKI 
      FIYHICRNV LRESVTKQT FTDAILGNS LRTYSDMLI 
      CHPSVKGFS AYRNSIKGS WYIQLLCKV FMEHAHDMD 
      IESLLRKVD EELEVVSSM PWVTENPNE GTQQTSTFQ 
      NIAFKKCYL HPGLYEEDG KMMKFPQNQ
    CDS sequence
    • >TC012579 RA
      atgttgccg gaacagaaa gacttaatt tacaaacac 
      ttccctcaa cttgtgcaa caatgcgat ttagaacag 
      attgtggaa attatgatc aagaagggc aatctcaat 
      caggacttt ttggataaa ctaccgctg gagtcagac 
      aaggcccac aataaccgc caaattttg ttcaaatta 
      ttaggtctg gaagaggtg aacactttt tcaggcctt 
      gtcgacggt ttaaggaag acgggatgc gcccgcttg 
      gccaatatt ttggagaac aaattagag acaatcatt 
      gagagtaac attttgcat cccctggac gtaaacatc 
      gatgtaaaa ttttcgacg cagttttac gacactgaa 
      aggtacaac cggactggt ttttactca actaggtct 
      aaaaatcgc ggttacgtc ctcataatc aacaacata 
      aattttgcc caaagctat gacgaaagg cacggggcg 
      gaggccgac gaacgcaac ctgcgccaa ttattcgaa 
      aaaatgggg ctgaccgtg ttttgctac agggacctg 
      aacgcttac gacatgtat tttaagaca cgagagttc 
      tccgagaaa ctgcctgtt actcaaccc gacattggt 
      atcgtcatt ataatgagc cacggctta gtcatcaac 
      aacgaaacc atgatagtg agcagcgac aacaaatac 
      ctggctgaa gaaagcttc aaaaaaatt ttcaacaac 
      gaaaactgc gcccttttc aggaggaag cccaagatt 
      tttatttac cacatttgc agaaacgtt ctcagggaa 
      tcagtgact aaacagacc ttcaccgat gccattttg 
      ggcaattcg ctcaggacg tacagcgac atgctcatc 
      tgccaccct tcagtaaag ggtttttcc gcttataga 
      aacagtatt aaaggctcg tggtacatt cagttatta 
      tgtaaagtt tttatggaa cacgcccat gacatggat 
      attgaatct ttgctcagg aaagttgat gaggagtta 
      gaagttgtc tcttccatg ccttgggta acagagaat 
      ccaaatgag gggactcaa caaacgtcc acttttcaa 
      aacattgcg ttcaaaaaa tgctatctt catccaggg 
      ttgtacgaa gaggacgga aaaatgatg aaatttccg 
      caaaatcag tga
    mRNA sequence
    • >TC012579 RA
      attctgtgg ttgagcacc gaaaatgcg caagcgccc 
      caaccagac tccgagtta ttgatttgt tttcatttc 
      aaattacaa aatatggat gagattaag ccaatttgc 
      ctcccattc cggacaaat ttggaaatt ggtccagcc 
      agacgtcac gatgacatc ttaaggtta gttaccgac 
      cgagcgcac aagtccgga acgcaccaa atttaaaca 
      caaatcggt tcggtatta attgttgta aatttctct 
      atcaaaaaa ttggaaaaa ctcatcgtt ttgcgaact 
      ggaacaatt ccaaaacac ctccaccac ttggctttt 
      gacagctat cagctgacg tttgacatt ttttgtgtt 
      agtttagtt attgtttgt gtaatctgt tctttataa 
      tttacccaa tgttgccgg aacagaaag acttaattt 
      acaaacact tccctcaac ttgtgcaac aatgcgatt 
      tagaacaga ttgtggaaa ttatgatca agaagggca 
      atctcaatc aggactttt tggataaac taccgctgg 
      agtcagaca aggcccaca ataaccgcc aaattttgt 
      tcaaattat taggtctgg aagaggtga acacttttt 
      caggccttg tcgacggtt taaggaaga cgggatgcg 
      cccgcttgg ccaatattt tggagaaca aattagaga 
      caatcattg agagtaaca ttttgcatc ccctggacg 
      taaacatcg atgtaaaat tttcgacgc agttttacg 
      acactgaaa ggtacaacc ggactggtt tttactcaa 
      ctaggtcta aaaatcgcg gttacgtcc tcataatca 
      acaacataa attttgccc aaagctatg acgaaaggc 
      acggggcgg aggccgacg aacgcaacc tgcgccaat 
      tattcgaaa aaatggggc tgaccgtgt tttgctaca 
      gggacctga acgcttacg acatgtatt ttaagacac 
      gagagttct ccgagaaac tgcctgtta ctcaacccg 
      acattggta tcgtcatta taatgagcc acggcttag 
      tcatcaaca acgaaacca tgatagtga gcagcgaca 
      acaaatacc tggctgaag aaagcttca aaaaaattt 
      tcaacaacg aaaactgcg cccttttca ggaggaagc 
      ccaagattt ttatttacc acatttgca gaaacgttc 
      tcagggaat cagtgacta aacagacct tcaccgatg 
      ccattttgg gcaattcgc tcaggacgt acagcgaca 
      tgctcatct gccaccctt cagtaaagg gtttttccg 
      cttatagaa acagtatta aaggctcgt ggtacattc 
      agttattat gtaaagttt ttatggaac acgcccatg 
      acatggata ttgaatctt tgctcagga aagttgatg 
      aggagttag aagttgtct cttccatgc cttgggtaa 
      cagagaatc caaatgagg ggactcaac aaacgtcca 
      cttttcaaa acattgcgt tcaaaaaat gctatcttc 
      atccagggt tgtacgaag aggacggaa aaatgatga 
      aatttccgc aaaatcagt gacgcttat tgaggtgct 
      aacatagcc tgtaaggtg caataataa ataacattc 
      ctatttttt attcgtgca atgtgtaat aaggttacg 
      caataaagc aaatgttta ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY