General information | |||
TC012565 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11521439..11525265 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012565 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07656 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012565 RA
MDASYVPNN PQFGLQLEC NWSSGPNAT LQSSACTDD LSSCWSEDM GSFSLPLDL EPLPSLFPF SPCNNTANY KSEAQNQPS PYKQPSSDM ADVLLSLKH AVVHPGQSP AEKYDQQHL YHPQVLLSP GGYSGSLCD QGFAQQPMF PSMSVNVSM NMTMHGYHP SSSYPANEI SCPQVQWSA TPQAGSTPT YQTQGLLSP SYGGTTYSF TADFRPPQE TPVLASYKS VPMSFSSPR RRHSINLLE EDNQKPNVC RICGKSYAR PSTLKTHLR THSGEKPYR CIDCNKSFS QAANLTAHV RTHSGEKPF RCPVCDRRF SQSSSVTTH MRTHSGERP YRCRLCKKA FSDSSTLTK HLRIHSGEK PYECKLCLL RFSQSGNLN RHMRVHGAA SMMPP
- >TC012565 RA
atggacgcc agttacgtc cctaacaac ccccagttc ggtctccaa ctcgaatgc aactggagc tctggcccc aacgccact ctccagtcc tcagcgtgc accgacgac ctctcctcc tgctggagc gaagacatg ggctctttc tccctgcct ctagacctg gagccgctg ccgagcctc ttccccttc tcgccttgc aacaacacc gccaactac aaaagcgaa gctcaaaac cagccctcc ccctacaag cagccctcg tccgacatg gccgacgtc ctcctctcc ctcaagcac gcggtggtg catcccggc cagagcccg gccgagaaa tacgaccag cagcacctc taccacccc caggtgctg ctctcgccg gggggctac tcggggagt ctctgcgac caggggttt gcccagcag cccatgttc cccagcatg tcggtcaac gtgtccatg aacatgacc atgcatggg taccatccg tctagttcc tacccagcg aatgaaatc tcgtgtccc caggtgcag tggagtgcg actccgcaa gctgggtcc acacccacg taccagact caaggactt ttgagtcct tcgtatgga gggacgact tattcgttc actgctgac tttaggcct ccgcaggaa acgcctgtt ctggcctcg tataagtca gtgcctatg tcgttctcc tcgccgagg aggaggcac agtattaat ctgctcgag gaggataat caaaagccg aatgtttgt aggatatgt ggcaaaagc tacgcccgg cccagcacc ctgaaaacc cacttgcgc acgcactca ggtgagaag ccctaccgc tgcatcgac tgcaacaaa tctttctct caggctgcc aacctgaca gcgcacgtc cggacccac tccggggaa aagcccttc cggtgtccc gtctgcgac cgccgcttc tcccagagc tcctcggtg acgacccac atgcgcacc cactccggg gagcggccc taccgctgc cgcctgtgc aagaaggcc ttctccgac agcagcacc ctcaccaag catctcagg atacacagc ggcgagaag ccctacgag tgcaaactg tgtcttttg cgcttcagc caaagcggc aacctcaac agacacatg cgggtgcac ggggctgcc agcatgatg cccccatga
- >TC012565 RA
catttccca aatctcgta cggcttctg ttttgcaac aggtgcgca agttcgccg tttgatttg gtccagatc cagcctcac gatggacgc cagttacgt ccctaacaa cccccagtt cggtctcca actcgaatg caactggag ctctggccc caacgccac tctccagtc ctcagcgtg caccgacga cctctcctc ctgctggag cgaagacat gggctcttt ctccctgcc tctagacct ggagccgct gccgagcct cttcccctt ctcgccttg caacaacac cgccaacta caaaagcga agctcaaaa ccagccctc cccctacaa gcagccctc gtccgacat ggccgacgt cctcctctc cctcaagca cgcggtggt gcatcccgg ccagagccc ggccgagaa atacgacca gcagcacct ctaccaccc ccaggtgct gctctcgcc ggggggcta ctcggggag tctctgcga ccaggggtt tgcccagca gcccatgtt ccccagcat gtcggtcaa cgtgtccat gaacatgac catgcatgg gtaccatcc gtctagttc ctacccagc gaatgaaat ctcgtgtcc ccaggtgca gtggagtgc gactccgca agctgggtc cacacccac gtaccagac tcaaggact tttgagtcc ttcgtatgg agggacgac ttattcgtt cactgctga ctttaggcc tccgcagga aacgcctgt tctggcctc gtataagtc agtgcctat gtcgttctc ctcgccgag gaggaggca cagtattaa tctgctcga ggaggataa tcaaaagcc gaatgtttg taggatatg tggcaaaag ctacgcccg gcccagcac cctgaaaac ccacttgcg cacgcactc aggtgagaa gccctaccg ctgcatcga ctgcaacaa atctttctc tcaggctgc caacctgac agcgcacgt ccggaccca ctccgggga aaagccctt ccggtgtcc cgtctgcga ccgccgctt ctcccagag ctcctcggt gacgaccca catgcgcac ccactccgg ggagcggcc ctaccgctg ccgcctgtg caagaaggc cttctccga cagcagcac cctcaccaa gcatctcag gatacacag cggcgagaa gccctacga gtgcaaact gtgtctttt gcgcttcag ccaaagcgg caacctcaa cagacacat gcgggtgca cggggctgc cagcatgat gcccccatg acaggggct gctcatctg aaacggctg ggcgttttg accacgctt gtgggatta gctagagct cggggaatt gcacaacaa ttaacaatg gtcggagag caaataact ggctttgcc aaaaatata agatttatt taagttaat gtgagggat ttgcgggca aatatttga ggtttcgtg aatgacggc tcaaaggcg actcaaaat tgcaactcg ggttcggtg caaattttc cgtgaaaga ggacgcaaa gtcgccaaa atttacgga gatgacaaa cagttttta ccgcttcta ttatgaaac ttatctcag aatatctcg cggataaaa aatccgacg aaaacaaat cataaaatg gaagaaaat aacatcaat ttacaaaat ttgtaggct acggttaat taattaatt gctaattga aatgtgaaa taaatttat tttagaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
