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Gene information
General information
TC011929 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11509350..11512327
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011929
    iBeetle screen iB_07655

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011929 RA
      MAEDDEIDI LGDFNLDNF LTKGDSGIY ENSSLVSQN 
      SDLLNCDYT IHPQWLLDK PSANPDCWY DNSSVLSPG 
      LSEKPDFGG SDDALGHVS TENSISDES GWTEKEKNL 
      LERGIEIFG KSNVRLSQF IGSRTPAEV KYYLKNFYL 
      EAQTSYNGF NTRVEDMNI VNNLVSDIL DDTQIPASI 
      EEVIAAVST AKPTVPIKQ ARKKKFSFS SIEDEDLSQ 
      GASPSLKSN VKEGAAKLD QKRKIKNKL KRPVKFKIR 
      TKLKFPMHN VKQKTVKIM NQENKKTEI GRVEITTGR 
      GLAVPICEG EEIVKISKD DSDSDVEID VEDSDEVKP 
      EIKVNKCVT ELPNTEIKE NDICESYSK PQKITIDLS 
      VLDESLVTE LTTLETPQS EVNFKENSI TELEKVVHS 
      EFFEGRPTK TPLRYLKIR NHIINCWLT SKPSYVTKT 
      SIRQGLRNC GDVNCLGRI HCYLEQIGA INYGCPQTN 
      YVRPLIDVF QSVANKNEK NLKDAKNQV KQNIELGCR 
      PRVKRKFAN DGEGGCTLT HDEKGDVIN TTIVNEEPA 
      KPKLSIKRP TIRLIYCRN FPEQKYKLR MHLTTLLLM 
      DFHAHTSLT EVMGLVAGT WNPTKKILT ISHYEPCRN 
      IASSATHCD MCPISQSKA AELIHNRGL DVLGWFHSH 
      PTFAPEPSQ QDLETQQDV QMWIGSKSP CLGVILSPF 
      SAHGALIAS PFRCMVVDK KENFEDQFV PYKFQVDVV 
      AIEADFDRF FSDLGRIFH FSKSNEGKI DFGKPYFQD 
      NSISYLEKY ITNDL
    CDS sequence
    • >TC011929 RA
      atggcggaa gatgacgaa atagatatt ttaggcgat 
      tttaattta gataacttt ttaacaaag ggcgattcc 
      ggcatttat gaaaactct tcgcttgtg tcccaaaac 
      tccgatctc ctgaactgt gattacaca atccacccg 
      cagtggctt ttggacaaa cctagcgct aacccggac 
      tgctggtac gataacagt tcagttttg tcgcctggg 
      ctttcagaa aaacccgat tttgggggc agcgatgac 
      gctctgggg cacgtctcc accgaaaac tccatttca 
      gacgagagc gggtggacg gaaaaggag aagaatttg 
      ctggaacga gggattgag atttttggg aagagcaat 
      gttaggctg tcgcagttt atcggctct cggacgccg 
      gctgaagtg aaatattac cttaaaaat ttttacttg 
      gaggcgcaa acttcgtat aatggcttt aacactaga 
      gtggaggac atgaatatt gtcaataat ttggtttcg 
      gatattttg gatgatact cagattcct gcgagtatt 
      gaggaggta atcgcagct gtgagcacc gcaaagccc 
      acagtaccc ataaagcag gcgcgcaag aagaaattt 
      tcgtttagt agcattgag gatgaagat ttatcacaa 
      ggggctagt ccctcgtta aaaagtaac gttaaggaa 
      ggtgcggct aagttggac caaaagagg aaaattaag 
      aataaactg aaacgtcca gtgaaattt aaaatcagg 
      acaaaacta aagtttcca atgcataac gtcaaacag 
      aaaactgtt aaaattatg aaccaggaa aataagaaa 
      actgagata gggagggtt gagatcacc actgggaga 
      gggttagct gtgccaatc tgtgaaggc gaggaaatc 
      gtgaaaata agcaaagac gattccgat tcagacgta 
      gagattgat gttgaagat tctgacgaa gttaaaccc 
      gaaattaaa gtaaataaa tgcgtcact gaattaccc 
      aacactgaa attaaggaa aatgatatt tgtgaaagc 
      tactcaaag ccgcaaaaa ataacaata gatttatca 
      gtattagac gagtcgctt gtgaccgaa cttacaact 
      ttggaaacg ccacaatcg gaagttaac ttcaaggaa 
      aattctatc acggaacta gagaaggtg gttcatagc 
      gagtttttc gagggacga cccactaaa acaccgttg 
      cgatatctc aaaatacgg aatcatata attaattgt 
      tggctcacg tcgaagcct tcctacgtt acgaagact 
      tcgattcgg caaggttta cgaaactgt ggcgatgtt 
      aactgtttg ggacgcatt cattgttat ttggaacaa 
      attggcgca attaattac ggatgtcca caaacaaat 
      tacgttcgc ccattaatt gatgtgttt caaagtgtt 
      gcaaataag aacgaaaaa aacttaaaa gatgccaaa 
      aatcaagtt aaacagaac attgaattg gggtgtagg 
      ccgagggtt aagcggaaa tttgctaat gacggggaa 
      gggggctgt actctcacc cacgacgag aaaggagac 
      gtcataaac accaccata gtgaacgaa gagccagcc 
      aaacccaaa ctgtccatc aaacggccc acaatacgc 
      ctcatctat tgtcgaaat ttccccgag caaaaatac 
      aagctccgg atgcacctc acaaccctc cttttgatg 
      gacttccac gcccacaca tccctcacc gaggtcatg 
      ggtctggtt gctgggact tggaacccg accaaaaaa 
      attctgacc atttctcat tacgaaccg tgccggaat 
      atcgcgtcc tcggccacg cattgcgac atgtgcccc 
      atttcgcag tcaaaagcc gccgaattg atccacaat 
      cgggggctg gacgtcttg gggtggttt cactcgcac 
      ccgacgttc gcccccgag ccgtcacag caggacctg 
      gagacgcaa caagacgtt caaatgtgg attgggagc 
      aaatccccg tgtttgggt gtgattttg tcgccgttt 
      agcgcacat ggggccctg attgcgtcc ccctttcgg 
      tgcatggtg gtggacaag aaggagaat tttgaggac 
      cagttcgtg ccttacaaa tttcaagtc gatgtggtt 
      gcaatcgag gcggatttt gaccggttt ttcagcgat 
      ttgggtcga atttttcat ttctcgaag tcaaacgag 
      gggaaaatc gacttcggc aagccgtac tttcaggat 
      aattccata agttattta gagaagtat atcactaat 
      gacttgtga
    mRNA sequence
    • >TC011929 RA
      atcaaaaca aaaatggcg gaagatgac gaaatagat 
      attttaggc gattttaat ttagataac tttttaaca 
      aagggcgat tccggcatt tatgaaaac tcttcgctt 
      gtgtcccaa aactccgat ctcctgaac tgtgattac 
      acaatccac ccgcagtgg cttttggac aaacctagc 
      gctaacccg gactgctgg tacgataac agttcagtt 
      ttgtcgcct gggctttca gaaaaaccc gattttggg 
      ggcagcgat gacgctctg gggcacgtc tccaccgaa 
      aactccatt tcagacgag agcgggtgg acggaaaag 
      gagaagaat ttgctggaa cgagggatt gagattttt 
      gggaagagc aatgttagg ctgtcgcag tttatcggc 
      tctcggacg ccggctgaa gtgaaatat taccttaaa 
      aatttttac ttggaggcg caaacttcg tataatggc 
      tttaacact agagtggag gacatgaat attgtcaat 
      aatttggtt tcggatatt ttggatgat actcagatt 
      cctgcgagt attgaggag gtaatcgca gctgtgagc 
      accgcaaag cccacagta cccataaag caggcgcgc 
      aagaagaaa ttttcgttt agtagcatt gaggatgaa 
      gatttatca caaggggct agtccctcg ttaaaaagt 
      aacgttaag gaaggtgcg gctaagttg gaccaaaag 
      aggaaaatt aagaataaa ctgaaacgt ccagtgaaa 
      tttaaaatc aggacaaaa ctaaagttt ccaatgcat 
      aacgtcaaa cagaaaact gttaaaatt atgaaccag 
      gaaaataag aaaactgag atagggagg gttgagatc 
      accactggg agagggtta gctgtgcca atctgtgaa 
      ggcgaggaa atcgtgaaa ataagcaaa gacgattcc 
      gattcagac gtagagatt gatgttgaa gattctgac 
      gaagttaaa cccgaaatt aaagtaaat aaatgcgtc 
      actgaatta cccaacact gaaattaag gaaaatgat 
      atttgtgaa agctactca aagccgcaa aaaataaca 
      atagattta tcagtatta gacgagtcg cttgtgacc 
      gaacttaca actttggaa acgccacaa tcggaagtt 
      aacttcaag gaaaattct atcacggaa ctagagaag 
      gtggttcat agcgagttt ttcgaggga cgacccact 
      aaaacaccg ttgcgatat ctcaaaata cggaatcat 
      ataattaat tgttggctc acgtcgaag ccttcctac 
      gttacgaag acttcgatt cggcaaggt ttacgaaac 
      tgtggcgat gttaactgt ttgggacgc attcattgt 
      tatttggaa caaattggc gcaattaat tacggatgt 
      ccacaaaca aattacgtt cgcccatta attgatgtg 
      tttcaaagt gttgcaaat aagaacgaa aaaaactta 
      aaagatgcc aaaaatcaa gttaaacag aacattgaa 
      ttggggtgt aggccgagg gttaagcgg aaatttgct 
      aatgacggg gaagggggc tgtactctc acccacgac 
      gagaaagga gacgtcata aacaccacc atagtgaac 
      gaagagcca gccaaaccc aaactgtcc atcaaacgg 
      cccacaata cgcctcatc tattgtcga aatttcccc 
      gagcaaaaa tacaagctc cggatgcac ctcacaacc 
      ctccttttg atggacttc cacgcccac acatccctc 
      accgaggtc atgggtctg gttgctggg acttggaac 
      ccgaccaaa aaaattctg accatttct cattacgaa 
      ccgtgccgg aatatcgcg tcctcggcc acgcattgc 
      gacatgtgc cccatttcg cagtcaaaa gccgccgaa 
      ttgatccac aatcggggg ctggacgtc ttggggtgg 
      tttcactcg cacccgacg ttcgccccc gagccgtca 
      cagcaggac ctggagacg caacaagac gttcaaatg 
      tggattggg agcaaatcc ccgtgtttg ggtgtgatt 
      ttgtcgccg tttagcgca catggggcc ctgattgcg 
      tcccccttt cggtgcatg gtggtggac aagaaggag 
      aattttgag gaccagttc gtgccttac aaatttcaa 
      gtcgatgtg gttgcaatc gaggcggat tttgaccgg 
      tttttcagc gatttgggt cgaattttt catttctcg 
      aagtcaaac gaggggaaa atcgacttc ggcaagccg 
      tactttcag gataattcc ataagttat ttagagaag 
      tatatcact aatgacttg tgattacat tatcgagaa 
      tatctcaaa tatttgtgc gaaataaca aggtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY