General information | |||
TC031594 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451355.1: 0..2873 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC031594 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07654 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031594 RA
MNKNSHDDN PYGVTASVA APVRTRRDV QSHTGNSRA SGTLQCSRP TRSNRNITS TSVNESSTS SDESGTFSE SPRRLALPN RRAPPLGNV RRRKKWSSD DNSDLLRAY LRATKYDTC KFGFRKRML IEWRLMRPE SVLTENQLA TQLTSIKRN NLVPEIITQ TIRTEEMER MRRETDNSN TIKTNNDTE NAEINVENE QPQIDKEEQ IETTRMDQE IETKRTELI NEDKIRIIN HFEVLKLSY QDTEANHRP SLRKIKPNK KVHTLLQYL NNYIRESTT DADFPNIEA VQTIVYCVA RAVTEEVVP EQPGERTIN KPSEVKVPK WKERIEKKI NRIRSTLNK INDWKKNKG GRKLRKEVK SIMKSMNLK MNDEHLDRN LAVKINEMK QKIAVFGKK LRRYNLSHK RKTQNKLFS KNEKTFYAS LNNTTTTPS TSNTVPIGE LEEFWRNIW KNKGDHKKE APWIVEEKQ RMNETPGME SCNITITDV HEVVRKLHN WKAPGPDKI QNFWWKNLT AVHSYLVRH INNIIEHPE ETPQFITLG ITYMKAKSE DLSKPQNYR PITCLNTLY KIITSLLAN KIDNHVDEN QILTEEQKG CRRKTQGCK EQLIIDGII ANEAKKRHR NVSVAWLDY QKAFDSVPH SYLIDVLNI YKVNGKIVN FLQHCMKSW RTQLLVNND RKTSVKTNP IPIESGIFQ GDSLSPILF IICLNPLSR LLQSSNLGY KLKGSSTQR LSHLLYVDD AKLYAENEQ DLGKLLTIT KTFSENIRM KFGYDKCAK IIYKKGKKT TSQNIEHLN IKELDDTEK YKYLGLDQH LIRDTKELK DKTEKKLHT RINKILNTS LNGKNKVKA VNTWAIPTI TYTFRNRYD SKPNPSHKC VSHQTLPTT TIRRKRTLE LGTPLEETN
- >TC031594 RA
atgaataaa aattcgcat gatgataat ccatacggc gtgacggcg agtgtggct gctcccgtt cggacacgt cgtgatgtt cagtcacac actggaaac agtagagcg agtggtact ctccagtgt tcacggccc acaagatcg aatcggaac ataacatct acttctgtc aatgagtca agcacatcc tcggatgag tcgggcaca ttctcagaa tccccaagg agactggcc ctccccaat aggagagcc cctccttta ggaaacgta agacgacgc aagaaatgg agtagtgac gataatagt gacctccta agagcgtac ctccgagca acaaaatat gacacgtgc aaattcgga ttcaggaaa cgtatgctg attgaatgg agattgatg agaccagaa agtgtacta actgaaaac caactagca acacaactg acatcaatc aaaagaaat aacttagta ccagagatc ataacacaa accatcaga actgaggaa atggaaaga atgagaaga gaaaccgat aattccaac actattaaa acaaacaat gatacagaa aatgctgag ataaatgtc gagaatgaa caaccacag atagataaa gaagaacaa atagaaaca acaagaatg gaccaagaa atagaaact aaaagaaca gaattaata aatgaagat aaaatccga attattaac cattttgaa gtcttgaaa ctctcctat caagacaca gaagctaat cacagaccc agcttgaga aaaataaaa ccaaacaag aaagtacat acacttctt caatactta aacaactat attcgtgag agtaccact gacgctgac tttccgaac atcgaagca gtgcagaca atcgtctat tgtgtagca agagcagtc acagaagaa gtagtacca gagcaacca ggagagaga acgataaac aaaccctct gaagtgaaa gtaccaaaa tggaaagaa agaatagag aagaaaatc aacagaata cggagcaca ctcaacaag ataaacgac tggaaaaag aacaaaggt ggacgaaaa ctgagaaag gaagtaaaa tcaattatg aagagtatg aacctaaag atgaatgac gaacatctt gacagaaac ttagcagta aaaataaat gagatgaaa cagaaaata gcagtattt ggaaagaag ttacgaaga tacaactta agtcacaaa agaaaaaca cagaacaaa ctcttctca aaaaatgaa aaaacattc tatgcatct ctaaacaac acaacaaca actcccagt acatcgaac acagtaccc attggagag cttgaagag ttctggcga aacatctgg aaaaacaaa ggggaccat aaaaaagaa gcaccatgg attgtagag gaaaaacaa agaatgaat gaaacacct ggaatggaa tcatgcaac atcacaata accgatgta catgaagtt gtccggaaa ctacacaat tggaaagca cccggccct gacaaaatc cagaacttc tggtggaaa aacttaacg gcagtacac tcatatcta gtacgacac ataaacaac atcattgaa cacccagaa gaaacacca caattcatc acactaggg ataacatat atgaaagca aaaagcgaa gatctttct aaacctcaa aactacaga cccataact tgtttgaac accctatac aaaatcata acatcactg ctcgcaaac aagatcgac aaccatgta gacgaaaat caaattcta acagaagaa caaaagggc tgtagacgg aagacccag gggtgcaaa gagcaattg attatagat ggcataatc gccaatgaa gcaaagaaa agacataga aatgtaagt gtagcctgg cttgactac caaaaagcc ttcgactct gtacctcat tcatactta atcgacgta cttaacatc tacaaagtc aatggcaaa attgttaac tttctccaa cattgcatg aagtcatgg cgaacacaa ctcttagtc aataatgac cgaaaaaca tccgtgaaa accaaccca attccgatt gaaagtggc atcttccaa ggagactcc ttgagccct atcttgttc ataatctgc ctaaatcca ctctcaaga ctgcttcaa tcctctaac cttggctac aaactaaaa ggctcatca acacaacga ctcagccac ctcttgtac gtcgacgat gccaagtta tatgcagaa aacgaacaa gatctcgga aaactgctc accatcaca aaaacattc agtgagaac atccgtatg aaatttgga tatgacaag tgtgccaag atcatctac aaaaaaggg aaaaaaacg accagccag aacattgaa cacctcaac atcaaggaa ttggacgac actgaaaag tacaaatac ttaggactg gaccaacat ctaatacga gacacaaaa gaactgaaa gataaaaca gagaagaaa ctacataca agaataaac aagatccta aacacaagt ctgaatgga aaaaacaaa gtgaaagcc gtaaacacc tgggcaata ccaacaata acctacaca tttaggaac cgatatgac agcaaaccg aatccatca cacaaatgc gtcagtcat cagactcta cctaccacg acgatccgg aggaagagg actcttgaa cttggaaca cctctggaa gaaactaac taa
- >TC031594 RA
acaccacca aacaccaaa aaagcaaaa tgaataaaa attcgcatg atgataatc catacggcg tgacggcga gtgtggctg ctcccgttc ggacacgtc gtgatgttc agtcacaca ctggaaaca gtagagcga gtggtactc tccagtgtt cacggccca caagatcga atcggaaca taacatcta cttctgtca atgagtcaa gcacatcct cggatgagt cgggcacat tctcagaat ccccaagga gactggccc tccccaata ggagagccc ctcctttag gaaacgtaa gacgacgca agaaatgga gtagtgacg ataatagtg acctcctaa gagcgtacc tccgagcaa caaaatatg acacgtgca aattcggat tcaggaaac gtatgctga ttgaatgga gattgatga gaccagaaa gtgtactaa ctgaaaacc aactagcaa cacaactga catcaatca aaagaaata acttagtac cagagatca taacacaaa ccatcagaa ctgaggaaa tggaaagaa tgagaagag aaaccgata attccaaca ctattaaaa caaacaatg atacagaaa atgctgaga taaatgtcg agaatgaac aaccacaga tagataaag aagaacaaa tagaaacaa caagaatgg accaagaaa tagaaacta aaagaacag aattaataa atgaagata aaatccgaa ttattaacc attttgaag tcttgaaac tctcctatc aagacacag aagctaatc acagaccca gcttgagaa aaataaaac caaacaaga aagtacata cacttcttc aatacttaa acaactata ttcgtgaga gtaccactg acgctgact ttccgaaca tcgaagcag tgcagacaa tcgtctatt gtgtagcaa gagcagtca cagaagaag tagtaccag agcaaccag gagagagaa cgataaaca aaccctctg aagtgaaag taccaaaat ggaaagaaa gaatagaga agaaaatca acagaatac ggagcacac tcaacaaga taaacgact ggaaaaaga acaaaggtg gacgaaaac tgagaaagg aagtaaaat caattatga agagtatga acctaaaga tgaatgacg aacatcttg acagaaact tagcagtaa aaataaatg agatgaaac agaaaatag cagtatttg gaaagaagt tacgaagat acaacttaa gtcacaaaa gaaaaacac agaacaaac tcttctcaa aaaatgaaa aaacattct atgcatctc taaacaaca caacaacaa ctcccagta catcgaaca cagtaccca ttggagagc ttgaagagt tctggcgaa acatctgga aaaacaaag gggaccata aaaaagaag caccatgga ttgtagagg aaaaacaaa gaatgaatg aaacacctg gaatggaat catgcaaca tcacaataa ccgatgtac atgaagttg tccggaaac tacacaatt ggaaagcac ccggccctg acaaaatcc agaacttct ggtggaaaa acttaacgg cagtacact catatctag tacgacaca taaacaaca tcattgaac acccagaag aaacaccac aattcatca cactaggga taacatata tgaaagcaa aaagcgaag atctttcta aacctcaaa actacagac ccataactt gtttgaaca ccctataca aaatcataa catcactgc tcgcaaaca agatcgaca accatgtag acgaaaatc aaattctaa cagaagaac aaaagggct gtagacgga agacccagg ggtgcaaag agcaattga ttatagatg gcataatcg ccaatgaag caaagaaaa gacatagaa atgtaagtg tagcctggc ttgactacc aaaaagcct tcgactctg tacctcatt catacttaa tcgacgtac ttaacatct acaaagtca atggcaaaa ttgttaact ttctccaac attgcatga agtcatggc gaacacaac tcttagtca ataatgacc gaaaaacat ccgtgaaaa ccaacccaa ttccgattg aaagtggca tcttccaag gagactcct tgagcccta tcttgttca taatctgcc taaatccac tctcaagac tgcttcaat cctctaacc ttggctaca aactaaaag gctcatcaa cacaacgac tcagccacc tcttgtacg tcgacgatg ccaagttat atgcagaaa acgaacaag atctcggaa aactgctca ccatcacaa aaacattca gtgagaaca tccgtatga aatttggat atgacaagt gtgccaaga tcatctaca aaaaaggga aaaaaacga ccagccaga acattgaac acctcaaca tcaaggaat tggacgaca ctgaaaagt acaaatact taggactgg accaacatc taatacgag acacaaaag aactgaaag ataaaacag agaagaaac tacatacaa gaataaaca agatcctaa acacaagtc tgaatggaa aaaacaaag tgaaagccg taaacacct gggcaatac caacaataa cctacacat ttaggaacc gatatgaca gcaaaccga atccatcac acaaatgcg tcagtcatc agactctac ctaccacga cgatccgga ggaagagga ctcttgaac ttggaacac ctctggaag aaactaact aaactgaaa ctgcaagaa tctgaagac ccatattac acatatgta ccacaacca gtcattgaa acagacagc tggaaagtg tactgggac atcacga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
