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Gene information
General information
TC031594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451355.1: 0..2873
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031594
      iBeetle screen iB_07654

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031594 RA
        MNKNSHDDN PYGVTASVA APVRTRRDV QSHTGNSRA 
        SGTLQCSRP TRSNRNITS TSVNESSTS SDESGTFSE 
        SPRRLALPN RRAPPLGNV RRRKKWSSD DNSDLLRAY 
        LRATKYDTC KFGFRKRML IEWRLMRPE SVLTENQLA 
        TQLTSIKRN NLVPEIITQ TIRTEEMER MRRETDNSN 
        TIKTNNDTE NAEINVENE QPQIDKEEQ IETTRMDQE 
        IETKRTELI NEDKIRIIN HFEVLKLSY QDTEANHRP 
        SLRKIKPNK KVHTLLQYL NNYIRESTT DADFPNIEA 
        VQTIVYCVA RAVTEEVVP EQPGERTIN KPSEVKVPK 
        WKERIEKKI NRIRSTLNK INDWKKNKG GRKLRKEVK 
        SIMKSMNLK MNDEHLDRN LAVKINEMK QKIAVFGKK 
        LRRYNLSHK RKTQNKLFS KNEKTFYAS LNNTTTTPS 
        TSNTVPIGE LEEFWRNIW KNKGDHKKE APWIVEEKQ 
        RMNETPGME SCNITITDV HEVVRKLHN WKAPGPDKI 
        QNFWWKNLT AVHSYLVRH INNIIEHPE ETPQFITLG 
        ITYMKAKSE DLSKPQNYR PITCLNTLY KIITSLLAN 
        KIDNHVDEN QILTEEQKG CRRKTQGCK EQLIIDGII 
        ANEAKKRHR NVSVAWLDY QKAFDSVPH SYLIDVLNI 
        YKVNGKIVN FLQHCMKSW RTQLLVNND RKTSVKTNP 
        IPIESGIFQ GDSLSPILF IICLNPLSR LLQSSNLGY 
        KLKGSSTQR LSHLLYVDD AKLYAENEQ DLGKLLTIT 
        KTFSENIRM KFGYDKCAK IIYKKGKKT TSQNIEHLN 
        IKELDDTEK YKYLGLDQH LIRDTKELK DKTEKKLHT 
        RINKILNTS LNGKNKVKA VNTWAIPTI TYTFRNRYD 
        SKPNPSHKC VSHQTLPTT TIRRKRTLE LGTPLEETN
      CDS sequence
      • >TC031594 RA
        atgaataaa aattcgcat gatgataat ccatacggc 
        gtgacggcg agtgtggct gctcccgtt cggacacgt 
        cgtgatgtt cagtcacac actggaaac agtagagcg 
        agtggtact ctccagtgt tcacggccc acaagatcg 
        aatcggaac ataacatct acttctgtc aatgagtca 
        agcacatcc tcggatgag tcgggcaca ttctcagaa 
        tccccaagg agactggcc ctccccaat aggagagcc 
        cctccttta ggaaacgta agacgacgc aagaaatgg 
        agtagtgac gataatagt gacctccta agagcgtac 
        ctccgagca acaaaatat gacacgtgc aaattcgga 
        ttcaggaaa cgtatgctg attgaatgg agattgatg 
        agaccagaa agtgtacta actgaaaac caactagca 
        acacaactg acatcaatc aaaagaaat aacttagta 
        ccagagatc ataacacaa accatcaga actgaggaa 
        atggaaaga atgagaaga gaaaccgat aattccaac 
        actattaaa acaaacaat gatacagaa aatgctgag 
        ataaatgtc gagaatgaa caaccacag atagataaa 
        gaagaacaa atagaaaca acaagaatg gaccaagaa 
        atagaaact aaaagaaca gaattaata aatgaagat 
        aaaatccga attattaac cattttgaa gtcttgaaa 
        ctctcctat caagacaca gaagctaat cacagaccc 
        agcttgaga aaaataaaa ccaaacaag aaagtacat 
        acacttctt caatactta aacaactat attcgtgag 
        agtaccact gacgctgac tttccgaac atcgaagca 
        gtgcagaca atcgtctat tgtgtagca agagcagtc 
        acagaagaa gtagtacca gagcaacca ggagagaga 
        acgataaac aaaccctct gaagtgaaa gtaccaaaa 
        tggaaagaa agaatagag aagaaaatc aacagaata 
        cggagcaca ctcaacaag ataaacgac tggaaaaag 
        aacaaaggt ggacgaaaa ctgagaaag gaagtaaaa 
        tcaattatg aagagtatg aacctaaag atgaatgac 
        gaacatctt gacagaaac ttagcagta aaaataaat 
        gagatgaaa cagaaaata gcagtattt ggaaagaag 
        ttacgaaga tacaactta agtcacaaa agaaaaaca 
        cagaacaaa ctcttctca aaaaatgaa aaaacattc 
        tatgcatct ctaaacaac acaacaaca actcccagt 
        acatcgaac acagtaccc attggagag cttgaagag 
        ttctggcga aacatctgg aaaaacaaa ggggaccat 
        aaaaaagaa gcaccatgg attgtagag gaaaaacaa 
        agaatgaat gaaacacct ggaatggaa tcatgcaac 
        atcacaata accgatgta catgaagtt gtccggaaa 
        ctacacaat tggaaagca cccggccct gacaaaatc 
        cagaacttc tggtggaaa aacttaacg gcagtacac 
        tcatatcta gtacgacac ataaacaac atcattgaa 
        cacccagaa gaaacacca caattcatc acactaggg 
        ataacatat atgaaagca aaaagcgaa gatctttct 
        aaacctcaa aactacaga cccataact tgtttgaac 
        accctatac aaaatcata acatcactg ctcgcaaac 
        aagatcgac aaccatgta gacgaaaat caaattcta 
        acagaagaa caaaagggc tgtagacgg aagacccag 
        gggtgcaaa gagcaattg attatagat ggcataatc 
        gccaatgaa gcaaagaaa agacataga aatgtaagt 
        gtagcctgg cttgactac caaaaagcc ttcgactct 
        gtacctcat tcatactta atcgacgta cttaacatc 
        tacaaagtc aatggcaaa attgttaac tttctccaa 
        cattgcatg aagtcatgg cgaacacaa ctcttagtc 
        aataatgac cgaaaaaca tccgtgaaa accaaccca 
        attccgatt gaaagtggc atcttccaa ggagactcc 
        ttgagccct atcttgttc ataatctgc ctaaatcca 
        ctctcaaga ctgcttcaa tcctctaac cttggctac 
        aaactaaaa ggctcatca acacaacga ctcagccac 
        ctcttgtac gtcgacgat gccaagtta tatgcagaa 
        aacgaacaa gatctcgga aaactgctc accatcaca 
        aaaacattc agtgagaac atccgtatg aaatttgga 
        tatgacaag tgtgccaag atcatctac aaaaaaggg 
        aaaaaaacg accagccag aacattgaa cacctcaac 
        atcaaggaa ttggacgac actgaaaag tacaaatac 
        ttaggactg gaccaacat ctaatacga gacacaaaa 
        gaactgaaa gataaaaca gagaagaaa ctacataca 
        agaataaac aagatccta aacacaagt ctgaatgga 
        aaaaacaaa gtgaaagcc gtaaacacc tgggcaata 
        ccaacaata acctacaca tttaggaac cgatatgac 
        agcaaaccg aatccatca cacaaatgc gtcagtcat 
        cagactcta cctaccacg acgatccgg aggaagagg 
        actcttgaa cttggaaca cctctggaa gaaactaac 
        taa
      mRNA sequence
      • >TC031594 RA
        acaccacca aacaccaaa aaagcaaaa tgaataaaa 
        attcgcatg atgataatc catacggcg tgacggcga 
        gtgtggctg ctcccgttc ggacacgtc gtgatgttc 
        agtcacaca ctggaaaca gtagagcga gtggtactc 
        tccagtgtt cacggccca caagatcga atcggaaca 
        taacatcta cttctgtca atgagtcaa gcacatcct 
        cggatgagt cgggcacat tctcagaat ccccaagga 
        gactggccc tccccaata ggagagccc ctcctttag 
        gaaacgtaa gacgacgca agaaatgga gtagtgacg 
        ataatagtg acctcctaa gagcgtacc tccgagcaa 
        caaaatatg acacgtgca aattcggat tcaggaaac 
        gtatgctga ttgaatgga gattgatga gaccagaaa 
        gtgtactaa ctgaaaacc aactagcaa cacaactga 
        catcaatca aaagaaata acttagtac cagagatca 
        taacacaaa ccatcagaa ctgaggaaa tggaaagaa 
        tgagaagag aaaccgata attccaaca ctattaaaa 
        caaacaatg atacagaaa atgctgaga taaatgtcg 
        agaatgaac aaccacaga tagataaag aagaacaaa 
        tagaaacaa caagaatgg accaagaaa tagaaacta 
        aaagaacag aattaataa atgaagata aaatccgaa 
        ttattaacc attttgaag tcttgaaac tctcctatc 
        aagacacag aagctaatc acagaccca gcttgagaa 
        aaataaaac caaacaaga aagtacata cacttcttc 
        aatacttaa acaactata ttcgtgaga gtaccactg 
        acgctgact ttccgaaca tcgaagcag tgcagacaa 
        tcgtctatt gtgtagcaa gagcagtca cagaagaag 
        tagtaccag agcaaccag gagagagaa cgataaaca 
        aaccctctg aagtgaaag taccaaaat ggaaagaaa 
        gaatagaga agaaaatca acagaatac ggagcacac 
        tcaacaaga taaacgact ggaaaaaga acaaaggtg 
        gacgaaaac tgagaaagg aagtaaaat caattatga 
        agagtatga acctaaaga tgaatgacg aacatcttg 
        acagaaact tagcagtaa aaataaatg agatgaaac 
        agaaaatag cagtatttg gaaagaagt tacgaagat 
        acaacttaa gtcacaaaa gaaaaacac agaacaaac 
        tcttctcaa aaaatgaaa aaacattct atgcatctc 
        taaacaaca caacaacaa ctcccagta catcgaaca 
        cagtaccca ttggagagc ttgaagagt tctggcgaa 
        acatctgga aaaacaaag gggaccata aaaaagaag 
        caccatgga ttgtagagg aaaaacaaa gaatgaatg 
        aaacacctg gaatggaat catgcaaca tcacaataa 
        ccgatgtac atgaagttg tccggaaac tacacaatt 
        ggaaagcac ccggccctg acaaaatcc agaacttct 
        ggtggaaaa acttaacgg cagtacact catatctag 
        tacgacaca taaacaaca tcattgaac acccagaag 
        aaacaccac aattcatca cactaggga taacatata 
        tgaaagcaa aaagcgaag atctttcta aacctcaaa 
        actacagac ccataactt gtttgaaca ccctataca 
        aaatcataa catcactgc tcgcaaaca agatcgaca 
        accatgtag acgaaaatc aaattctaa cagaagaac 
        aaaagggct gtagacgga agacccagg ggtgcaaag 
        agcaattga ttatagatg gcataatcg ccaatgaag 
        caaagaaaa gacatagaa atgtaagtg tagcctggc 
        ttgactacc aaaaagcct tcgactctg tacctcatt 
        catacttaa tcgacgtac ttaacatct acaaagtca 
        atggcaaaa ttgttaact ttctccaac attgcatga 
        agtcatggc gaacacaac tcttagtca ataatgacc 
        gaaaaacat ccgtgaaaa ccaacccaa ttccgattg 
        aaagtggca tcttccaag gagactcct tgagcccta 
        tcttgttca taatctgcc taaatccac tctcaagac 
        tgcttcaat cctctaacc ttggctaca aactaaaag 
        gctcatcaa cacaacgac tcagccacc tcttgtacg 
        tcgacgatg ccaagttat atgcagaaa acgaacaag 
        atctcggaa aactgctca ccatcacaa aaacattca 
        gtgagaaca tccgtatga aatttggat atgacaagt 
        gtgccaaga tcatctaca aaaaaggga aaaaaacga 
        ccagccaga acattgaac acctcaaca tcaaggaat 
        tggacgaca ctgaaaagt acaaatact taggactgg 
        accaacatc taatacgag acacaaaag aactgaaag 
        ataaaacag agaagaaac tacatacaa gaataaaca 
        agatcctaa acacaagtc tgaatggaa aaaacaaag 
        tgaaagccg taaacacct gggcaatac caacaataa 
        cctacacat ttaggaacc gatatgaca gcaaaccga 
        atccatcac acaaatgcg tcagtcatc agactctac 
        ctaccacga cgatccgga ggaagagga ctcttgaac 
        ttggaacac ctctggaag aaactaact aaactgaaa 
        ctgcaagaa tctgaagac ccatattac acatatgta 
        ccacaacca gtcattgaa acagacagc tggaaagtg 
        tactgggac atcacga
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