Status
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Gene information
General information
TC012544 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11196164..11203968
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012544
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07650

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012544 RA
      MDPLYLALS LFRRRKFDK CAEVCSEIL ESQPLDQAA 
      WCLKMRALT QRVYVDDIE SEDLYEGDF LDDNAVATA 
      PRPGTSIKT AAPTTSVRP NTSTGRPIS GVARPGTQL 
      RPGSALDRL KTARPASQS ARAIRLGTA AMYSQKEGP 
      FIQVSRLNI AKYAKNSGL SKPLFEYLF YHEGDVRNA 
      MELAVQATQ FCRFEDWWW KVQLGKCYI ALNLIRDAE 
      QQMRSALKQ HHHVESFIR LTRIYLRLD QPLSAVEIC 
      KAGLEIFPN DVSIMTELA RLYENLNET APSVKFYRS 
      VVIEDAMNT EAIACIGMY HFYNNQPEM ALRYYRRVL 
      AMGAHSAEL YNNLGLCCL YSQQLDLTT SLFQRALDL 
      AIDPLIKAE VWYNLSHLA LSAGDLELA VQCLNLCLS 
      SDSTHASAF NNLAVLHHK MGRINLAKA YFTSAAALD 
      PNLYEPKTN LEFLQSV
    • >TC012544 RB
      MDPLYLALS LFRRRKFDK CAEVCSEIL ESQPLDQAA 
      WCLKMRALT QRVYVDDIE SEDLYEGDF LDDNAVATA 
      PRPGTSIKT AAPTTSVRP NTSTGRPIS GVARPGTQL 
      RPGSALDRL KTARPASQS ARAIRLGTA AMYSQKEGP 
      FIQVSRLNI AKYAKNSGL SKPLFEYLF YHEGDVRNA 
      MELAVQATQ FCRFEDWWW KVQLGKCYI ALNLIRDAE 
      QQMRSALKQ HHHVESFIR LTRIYLRLD QPLSAVEIC 
      KAGLEIFPN DVSIMTELA RLYENLNET APSVKFYRS 
      VVIEDAMNT EAIACIGMY HFYNNQPEM ALRYYRRVL 
      AMGAHSAEL YNNLGLCCL YSQQLDLTT SLFQRALDL 
      AIDPLIKAE VWYNLSHLA LSAGDLELA VQCLNLCLS 
      SDSTHASAF NNLAVLHHK MGRINLAKA YFTSAAALD 
      PNLYEPKTN LEFLQIPRR GGTASALGR GTTKLAARS 
      VRPAALAAR GGAPRTGRF RAQNRTGRA HSSDVLPRS 
      VPDRSRVEI GNLVEEHLC RLIETALSG SAKARVWCL 
      WVLCVVNSP ELGIAPEWG CGGTVFRIF LSAHGEDSF 
      TERALICPN SKELCQRSE HCFNLH
    CDS sequence
    • >TC012544 RA
      atggatccc ttataccta gccttaagt ttatttcgg 
      cgtcgaaaa ttcgacaaa tgtgccgaa gtgtgcagc 
      gaaattttg gaaagccag cccttggac caagcggcc 
      tggtgcctg aaaatgcgg gctctgacc cagagggtg 
      tacgtggac gacatcgag agcgaggac ttgtacgaa 
      ggcgacttc ctggacgac aacgccgtc gcgacagcc 
      ccgcgccca gggacctcc atcaaaacc gcagccccg 
      accacctca gtcaggccc aacacctcc accgggcgc 
      cccatctcg ggggtggcc cgccccggg acccagctg 
      cggcctggc tcggccctg gaccgcctc aagaccgcc 
      cggcccgcc agccagtcc gccagggcc atccggctg 
      ggcaccgcg gccatgtac agccagaaa gagggccct 
      tttatccaa gtttcccgc ctgaacatc gccaaatat 
      gcgaaaaac tcgggcttg tccaagccc ctgttcgag 
      taccttttc taccacgag ggcgatgtg agaaatgcg 
      atggagttg gcggtccaa gcgacccag ttctgcaga 
      tttgaggac tggtggtgg aaggtgcag ctgggcaag 
      tgctacatt gccctcaat ttgatcagg gatgcggaa 
      caacaaatg agaagtgcc ttgaaacag caccatcac 
      gttgagagt tttattaga ctgactagg atttatctg 
      agattggac caacctctc tccgctgtg gaaatatgc 
      aaagccggg cttgagatt tttcccaat gatgtttcg 
      ataatgacc gaactggca aggctttat gaaaatttg 
      aacgaaacg gcgccttcg gtcaagttt tacagaagt 
      gtggtcatt gaagatgcc atgaacacc gaagctatt 
      gcctgtatc gggatgtat cacttctat aacaaccag 
      ccggagatg gccttacgc tactacagg cgcgttttg 
      gccatgggt gcgcattcg gccgagctg tacaacaac 
      ctaggcttg tgctgccta tactcccag cagctcgac 
      ttgaccacc tcgctgttc cagcgcgcg cttgatttg 
      gcaatagat cccctaatc aaggctgag gtttggtac 
      aatttgtca catttggct ctctcagcc ggtgattta 
      gagctggct gtgcaatgt ttgaattta tgtctatcc 
      tcggattca acacacgca tcggcgttc aacaattta 
      gctgtttta catcacaag atggggcga ataaatctt 
      gctaaagca tatttcacg tcggctgca gctttggat 
      ccgaatttg tacgaaccg aaaactaat ttggaattt 
      ttacaaagt gtctaa
    • >TC012544 RB
      atggatccc ttataccta gccttaagt ttatttcgg 
      cgtcgaaaa ttcgacaaa tgtgccgaa gtgtgcagc 
      gaaattttg gaaagccag cccttggac caagcggcc 
      tggtgcctg aaaatgcgg gctctgacc cagagggtg 
      tacgtggac gacatcgag agcgaggac ttgtacgaa 
      ggcgacttc ctggacgac aacgccgtc gcgacagcc 
      ccgcgccca gggacctcc atcaaaacc gcagccccg 
      accacctca gtcaggccc aacacctcc accgggcgc 
      cccatctcg ggggtggcc cgccccggg acccagctg 
      cggcctggc tcggccctg gaccgcctc aagaccgcc 
      cggcccgcc agccagtcc gccagggcc atccggctg 
      ggcaccgcg gccatgtac agccagaaa gagggccct 
      tttatccaa gtttcccgc ctgaacatc gccaaatat 
      gcgaaaaac tcgggcttg tccaagccc ctgttcgag 
      taccttttc taccacgag ggcgatgtg agaaatgcg 
      atggagttg gcggtccaa gcgacccag ttctgcaga 
      tttgaggac tggtggtgg aaggtgcag ctgggcaag 
      tgctacatt gccctcaat ttgatcagg gatgcggaa 
      caacaaatg agaagtgcc ttgaaacag caccatcac 
      gttgagagt tttattaga ctgactagg atttatctg 
      agattggac caacctctc tccgctgtg gaaatatgc 
      aaagccggg cttgagatt tttcccaat gatgtttcg 
      ataatgacc gaactggca aggctttat gaaaatttg 
      aacgaaacg gcgccttcg gtcaagttt tacagaagt 
      gtggtcatt gaagatgcc atgaacacc gaagctatt 
      gcctgtatc gggatgtat cacttctat aacaaccag 
      ccggagatg gccttacgc tactacagg cgcgttttg 
      gccatgggt gcgcattcg gccgagctg tacaacaac 
      ctaggcttg tgctgccta tactcccag cagctcgac 
      ttgaccacc tcgctgttc cagcgcgcg cttgatttg 
      gcaatagat cccctaatc aaggctgag gtttggtac 
      aatttgtca catttggct ctctcagcc ggtgattta 
      gagctggct gtgcaatgt ttgaattta tgtctatcc 
      tcggattca acacacgca tcggcgttc aacaattta 
      gctgtttta catcacaag atggggcga ataaatctt 
      gctaaagca tatttcacg tcggctgca gctttggat 
      ccgaatttg tacgaaccg aaaactaat ttggaattt 
      ttacaaatc ccaaggcgc ggtggtacc gcgtccgcg 
      ctgggccgc ggtaccact aagttggct gcgcggagt 
      gtccgccca gcggccctg gcagcgcgt ggaggtgcc 
      ccccgaacg ggccgcttc cgcgcgcag aaccggact 
      gggcgggcg cattccagc gatgttttg ccgcggagt 
      gtacctgac cgctccaga gttgaaata gggaattta 
      gtcgaagaa cacctttgc cggttgata gagacggcg 
      ctctctggc tctgcgaaa gcacgtgtg tggtgcctg 
      tgggtgctc tgtgttgtg aattcaccc gaattggga 
      attgcgccg gaatggggc tgcggaggg acggttttt 
      cggattttt ctttcggca catggtgag gattctttc 
      acagaacgc gcgcttatt tgcccaaat tccaaggaa 
      ctgtgtcag cgatcagag cattgcttc aatttacac 
      tga
    mRNA sequence
    • >TC012544 RA
      cgcaccaaa atggatccc ttataccta gccttaagt 
      ttatttcgg cgtcgaaaa ttcgacaaa tgtgccgaa 
      gtgtgcagc gaaattttg gaaagccag cccttggac 
      caagcggcc tggtgcctg aaaatgcgg gctctgacc 
      cagagggtg tacgtggac gacatcgag agcgaggac 
      ttgtacgaa ggcgacttc ctggacgac aacgccgtc 
      gcgacagcc ccgcgccca gggacctcc atcaaaacc 
      gcagccccg accacctca gtcaggccc aacacctcc 
      accgggcgc cccatctcg ggggtggcc cgccccggg 
      acccagctg cggcctggc tcggccctg gaccgcctc 
      aagaccgcc cggcccgcc agccagtcc gccagggcc 
      atccggctg ggcaccgcg gccatgtac agccagaaa 
      gagggccct tttatccaa gtttcccgc ctgaacatc 
      gccaaatat gcgaaaaac tcgggcttg tccaagccc 
      ctgttcgag taccttttc taccacgag ggcgatgtg 
      agaaatgcg atggagttg gcggtccaa gcgacccag 
      ttctgcaga tttgaggac tggtggtgg aaggtgcag 
      ctgggcaag tgctacatt gccctcaat ttgatcagg 
      gatgcggaa caacaaatg agaagtgcc ttgaaacag 
      caccatcac gttgagagt tttattaga ctgactagg 
      atttatctg agattggac caacctctc tccgctgtg 
      gaaatatgc aaagccggg cttgagatt tttcccaat 
      gatgtttcg ataatgacc gaactggca aggctttat 
      gaaaatttg aacgaaacg gcgccttcg gtcaagttt 
      tacagaagt gtggtcatt gaagatgcc atgaacacc 
      gaagctatt gcctgtatc gggatgtat cacttctat 
      aacaaccag ccggagatg gccttacgc tactacagg 
      cgcgttttg gccatgggt gcgcattcg gccgagctg 
      tacaacaac ctaggcttg tgctgccta tactcccag 
      cagctcgac ttgaccacc tcgctgttc cagcgcgcg 
      cttgatttg gcaatagat cccctaatc aaggctgag 
      gtttggtac aatttgtca catttggct ctctcagcc 
      ggtgattta gagctggct gtgcaatgt ttgaattta 
      tgtctatcc tcggattca acacacgca tcggcgttc 
      aacaattta gctgtttta catcacaag atggggcga 
      ataaatctt gctaaagca tatttcacg tcggctgca 
      gctttggat ccgaatttg tacgaaccg aaaactaat 
      ttggaattt ttacaaagt gtctaatgt tgttttata 
      ataaataca gtttgtgac a
    • >TC012544 RB
      cgcaccaaa atggatccc ttataccta gccttaagt 
      ttatttcgg cgtcgaaaa ttcgacaaa tgtgccgaa 
      gtgtgcagc gaaattttg gaaagccag cccttggac 
      caagcggcc tggtgcctg aaaatgcgg gctctgacc 
      cagagggtg tacgtggac gacatcgag agcgaggac 
      ttgtacgaa ggcgacttc ctggacgac aacgccgtc 
      gcgacagcc ccgcgccca gggacctcc atcaaaacc 
      gcagccccg accacctca gtcaggccc aacacctcc 
      accgggcgc cccatctcg ggggtggcc cgccccggg 
      acccagctg cggcctggc tcggccctg gaccgcctc 
      aagaccgcc cggcccgcc agccagtcc gccagggcc 
      atccggctg ggcaccgcg gccatgtac agccagaaa 
      gagggccct tttatccaa gtttcccgc ctgaacatc 
      gccaaatat gcgaaaaac tcgggcttg tccaagccc 
      ctgttcgag taccttttc taccacgag ggcgatgtg 
      agaaatgcg atggagttg gcggtccaa gcgacccag 
      ttctgcaga tttgaggac tggtggtgg aaggtgcag 
      ctgggcaag tgctacatt gccctcaat ttgatcagg 
      gatgcggaa caacaaatg agaagtgcc ttgaaacag 
      caccatcac gttgagagt tttattaga ctgactagg 
      atttatctg agattggac caacctctc tccgctgtg 
      gaaatatgc aaagccggg cttgagatt tttcccaat 
      gatgtttcg ataatgacc gaactggca aggctttat 
      gaaaatttg aacgaaacg gcgccttcg gtcaagttt 
      tacagaagt gtggtcatt gaagatgcc atgaacacc 
      gaagctatt gcctgtatc gggatgtat cacttctat 
      aacaaccag ccggagatg gccttacgc tactacagg 
      cgcgttttg gccatgggt gcgcattcg gccgagctg 
      tacaacaac ctaggcttg tgctgccta tactcccag 
      cagctcgac ttgaccacc tcgctgttc cagcgcgcg 
      cttgatttg gcaatagat cccctaatc aaggctgag 
      gtttggtac aatttgtca catttggct ctctcagcc 
      ggtgattta gagctggct gtgcaatgt ttgaattta 
      tgtctatcc tcggattca acacacgca tcggcgttc 
      aacaattta gctgtttta catcacaag atggggcga 
      ataaatctt gctaaagca tatttcacg tcggctgca 
      gctttggat ccgaatttg tacgaaccg aaaactaat 
      ttggaattt ttacaaatc ccaaggcgc ggtggtacc 
      gcgtccgcg ctgggccgc ggtaccact aagttggct 
      gcgcggagt gtccgccca gcggccctg gcagcgcgt 
      ggaggtgcc ccccgaacg ggccgcttc cgcgcgcag 
      aaccggact gggcgggcg cattccagc gatgttttg 
      ccgcggagt gtacctgac cgctccaga gttgaaata 
      gggaattta gtcgaagaa cacctttgc cggttgata 
      gagacggcg ctctctggc tctgcgaaa gcacgtgtg 
      tggtgcctg tgggtgctc tgtgttgtg aattcaccc 
      gaattggga attgcgccg gaatggggc tgcggaggg 
      acggttttt cggattttt ctttcggca catggtgag 
      gattctttc acagaacgc gcgcttatt tgcccaaat 
      tccaaggaa ctgtgtcag cgatcagag cattgcttc 
      aatttacac tgacgccga tttacagtg aaatttctt 
      tataatgag tcgtgccca tcacagatt aacgcgcct 
      ctcgcatgg atggacacg gattatttc atccgagcg 
      cttttttaa tcacataat aaaattacg gctttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY