Status
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Gene information
General information
TC011944 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11185841..11193042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011944
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07649

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011944 RA
      MEIGHESSH KDLDAKSEK SLDEAKESK VELHESLDE 
      YDLEILHDI FAKQSGKGF DLPTLKEIL ENVGKLQLS 
      DEDFNVIFM RMNTARNGF VNWNEFISF LILCYEQKD 
      VKAEFETLE NPIPVAATL VRSNHRYTI NRITYCPTV 
      RPDRTISWH DGSFMTCSR DGTINYWSL DVQFERSVK 
      STCPNLRVQ QTWVTDMVA LPDVSVVCT SSTERDLRF 
      YDTSARKFE LRVIFTSIE CAIYSMNYW FSDDPNEES 
      KLVLGDMGG NIHVIFIES FARGPFRSQ LGLPLLEVR 
      YEKVIKGLV DGFRVKILP KQHSNVILQ IQYYHRLNT 
      IISCSECPK VGLLMRDLA EDKGKYVYN LPKGAWCFS 
      VDGTQMLAS GGSDCLVRL WNPFVPQNP IAVLYGHHT 
      AISSMILQD DAKFLYSLS QDKSIRVWD VAKQTCVQN 
      YLGIPQELG EHSDMTTLY NPESRQWII GSTMLAVIP 
      LSPKQSGEH TDGFTHTAG VSVVLYNPL FKIIVTCGL 
      DSHIIVWNP WDGRRMIAI KEAHTKSVH AEIIPIEIT 
      AASFDPGFQ RLVTGAHDG SLKIWNFNT GTCLRNMKI 
      DQNAEIKCI IWVKHRILV MGWNRRVTE FGDSGEVVA 
      VGGQFHKNW DLRHKEDIS SAAVRIPQT IVTGTYDGE 
      LIMWRLETG QPYKQFNAA DPTTRIKIQ YRKLKHKVE 
      AKVNKFSKK NIIARALGK PIEKEKKEP PKPPPAEEE 
      IVRPNVSRR LSAVQKLIG QSSTSSRIP SRARRVSTV 
      VMPEQCMPL RRLAIHSML FLNSRPMDP EVGTLLISL 
      ENGAIQVWS HHMSGGFIT SFSAIHKAG DYVICMATD 
      EKNDYLFTG SSVGYIKTW LLRNYCEPE TETISMPKL 
      RLQFPFLWG DFFAGRAAR IANTQSLPM LLNSYKGHV 
      MTISHLTYI DDAQILISA SADYTARMW TLSGRYLQT 
      LGTFKKWKT VPQGHEARP EEFQFTTPP DIERVGSST 
      TLRVLRGGA TLRKMTFKQ LQAKEVKEL THVDHSKIY 
      GNRLEEPIL GHYYKPSVR TTQFHEIKF DTSFPYIPV 
      YQHLIMPPS HDLTLTSLK PKSQEALQE VKKTPKEEA 
      EKKDKESKR SSASK
    CDS sequence
    • >TC011944 RA
      atggaaatc gggcacgaa tcctcgcac aaagacctc 
      gacgccaag tctgaaaag tccctcgac gaggcaaaa 
      gaatcaaag gtcgagctc cacgagagc ttggacgag 
      tacgacttg gaaatcctc cacgacatt ttcgccaaa 
      cagtcaggc aaaggcttc gacttgccc accttgaaa 
      gaaatcctc gagaacgtc ggcaagctc caactctcc 
      gacgaggac ttcaacgta atcttcatg cgcatgaac 
      accgcgaga aacggcttc gtcaactgg aacgaattc 
      atctcgttc ctgatcctc tgctacgaa cagaaggac 
      gtgaaggcc gagttcgag accttggag aaccccatc 
      ccggtggcc gccacgctg gtccgcagc aaccaccgc 
      tacaccatc aacaggatc acctactgc cccacggtg 
      cgccccgac aggaccatc agctggcac gacggcagc 
      ttcatgacc tgcagccgg gacggcacc atcaactac 
      tggtcgctg gacgtccag ttcgagcgg tcggtcaag 
      tccacgtgc cccaacctc cgcgtccag cagacgtgg 
      gtcaccgac atggtggcc ctccccgac gtcagcgtc 
      gtctgcacc tcgagcacc gagcgcgac ttgcgcttc 
      tacgacacc agtgcgcga aagttcgag ttgcgggtg 
      atcttcacc tcgatcgag tgtgcgatt tactccatg 
      aactactgg ttcagtgac gacccgaac gaggagtcc 
      aagctggtg ctgggggac atggggggc aacatccac 
      gtcatcttc atagagtcc ttcgcccgc gggcccttc 
      cgcagccag ctgggcctg ccgctgctg gaagtccgg 
      tacgagaag gtgatcaag ggcctggtg gacggcttc 
      cgggtcaaa atcctcccc aagcagcac tcaaacgtc 
      attctgcaa atccagtac taccaccgg ctcaacacc 
      atcatctcg tgctccgag tgccccaag gtggggctc 
      ctgatgcgg gacctggcc gaggacaag ggcaaatac 
      gtgtacaac ctgcccaag ggcgcgtgg tgcttctcg 
      gtcgatggc acccagatg ctggcaagt ggtggctcc 
      gattgcttg gtgcggctg tggaacccg ttcgttccc 
      cagaacccc attgcagtg ctgtatggg caccatacg 
      gccatttcc tcaatgatt ttgcaggat gatgcaaaa 
      tttttgtat agtttgtct caggataag agtatcagg 
      gtttgggac gtggcgaag caaacctgc gtccagaac 
      tatctcggg ataccgcag gagttgggg gagcacagc 
      gacatgacc acgctttac aatccagag agccggcaa 
      tggattatc gggagtacc atgctggcc gttatccca 
      ctcagtccc aagcaaagt ggggaacac accgatggg 
      ttcacacac actgctggg gtttcggtg gtactgtat 
      aacccactt ttcaagata atcgtcact tgcggcctg 
      gacagccac atcatcgtg tggaatccc tgggatggc 
      cgtcgcatg atcgccatc aaagaagcc cacacgaaa 
      agcgtccac gccgagata atccctatt gagataacg 
      gccgccagt tttgacccc ggatttcag cgtctagta 
      acaggtgcc cacgacggt tccctaaaa atttggaac 
      ttcaacact ggaacctgc cttcgcaac atgaaaatt 
      gaccaaaac gccgaaatc aaatgcatc atttgggtg 
      aaacaccgc attctggtc atgggctgg aaccgaagg 
      gtgacagag ttcggggac agtggcgaa gttgtggcc 
      gtgggagga cagtttcac aaaaactgg gacttgagg 
      cataaggag gacatcagc tctgctgcc gtaaggata 
      cctcagact atagtcaca gggacctac gacggcgag 
      ctgatcatg tggcggctt gagacaggg cagccctac 
      aagcagttt aatgccgcc gatcccacc accaggatt 
      aaaattcag tacagaaaa ctgaagcac aaagtggag 
      gcaaaagtg aacaaattc agcaagaag aacatcatc 
      gcgcgagcg ttgggcaaa cccatcgag aaggagaag 
      aaggaaccg ccgaaaccg cctcccgcc gaagaggaa 
      atcgtccga cctaacgtc tcgcgccgc ctctccgcc 
      gtgcagaag ctcatcggg cagagctcg acgtcgagc 
      cggatccct tcaagggcc cgccgcgtc tcgaccgtg 
      gtgatgccc gagcagtgc atgcctctg agacgcctc 
      gccatccac tcgatgctg ttcctcaac agccggccg 
      atggacccc gaggtcggg acgctcctc atctcgctg 
      gagaacggg gccatccag gtgtggtcc caccacatg 
      agcggcggc ttcataacg tccttctcg gccatacac 
      aaggccggg gactacgtc atttgcatg gccaccgac 
      gagaaaaac gactacctc ttcacgggc tccagtgtg 
      ggttacatc aaaacgtgg cttttgcgc aactactgc 
      gagcccgaa acggaaacg ataagcatg ccgaaactg 
      cgactgcag ttcccgttt ctgtggggc gacttcttc 
      gccggccgc gccgccagg atcgcaaat acccaaagc 
      ctgcccatg cttttgaac agctacaaa gggcacgtt 
      atgacgatt tcgcatttg acgtacatt gacgacgcc 
      caaattttg ataagtgcc agtgctgat tacacggcg 
      cggatgtgg acgctgagc ggtcgctat ttgcaaact 
      ttggggacg tttaagaag tggaaaact gttcctcaa 
      ggccacgaa gcgcggcct gaggagttc cagttcacg 
      actcctcca gacatcgaa agagttgga agttcgacg 
      actctgcga gtgcttcga ggtggtgcc actctgcgt 
      aagatgacg ttcaagcaa ctgcaagcc aaggaagtg 
      aaggagtta acgcatgtg gaccacagc aagatttac 
      ggcaacagg ttggaggaa ccgatcttg gggcattac 
      tacaagccc tcagttagg acgacgcag tttcacgaa 
      attaagttc gatacgtcg tttccttat attcctgtt 
      tatcaacat ttaatcatg cccccgtcg catgatctg 
      actctgact agtctcaag ccaaagtca caagaggcg 
      ttacaggaa gtgaagaaa accccgaaa gaagaagcg 
      gaaaagaag gataaggaa agcaaacgc tcctctgct 
      tcgaaataa
    mRNA sequence
    • >TC011944 RA
      aaaatttgg ccacttgtt tgtcactca aatgacagt 
      tgaaagatg gaaatcggg cacgaatcc tcgcacaaa 
      gacctcgac gccaagtct gaaaagtcc ctcgacgag 
      gcaaaagaa tcaaaggtc gagctccac gagagcttg 
      gacgagtac gacttggaa atcctccac gacattttc 
      gccaaacag tcaggcaaa ggcttcgac ttgcccacc 
      ttgaaagaa atcctcgag aacgtcggc aagctccaa 
      ctctccgac gaggacttc aacgtaatc ttcatgcgc 
      atgaacacc gcgagaaac ggcttcgtc aactggaac 
      gaattcatc tcgttcctg atcctctgc tacgaacag 
      aaggacgtg aaggccgag ttcgagacc ttggagaac 
      cccatcccg gtggccgcc acgctggtc cgcagcaac 
      caccgctac accatcaac aggatcacc tactgcccc 
      acggtgcgc cccgacagg accatcagc tggcacgac 
      ggcagcttc atgacctgc agccgggac ggcaccatc 
      aactactgg tcgctggac gtccagttc gagcggtcg 
      gtcaagtcc acgtgcccc aacctccgc gtccagcag 
      acgtgggtc accgacatg gtggccctc cccgacgtc 
      agcgtcgtc tgcacctcg agcaccgag cgcgacttg 
      cgcttctac gacaccagt gcgcgaaag ttcgagttg 
      cgggtgatc ttcacctcg atcgagtgt gcgatttac 
      tccatgaac tactggttc agtgacgac ccgaacgag 
      gagtccaag ctggtgctg ggggacatg gggggcaac 
      atccacgtc atcttcata gagtccttc gcccgcggg 
      cccttccgc agccagctg ggcctgccg ctgctggaa 
      gtccggtac gagaaggtg atcaagggc ctggtggac 
      ggcttccgg gtcaaaatc ctccccaag cagcactca 
      aacgtcatt ctgcaaatc cagtactac caccggctc 
      aacaccatc atctcgtgc tccgagtgc cccaaggtg 
      gggctcctg atgcgggac ctggccgag gacaagggc 
      aaatacgtg tacaacctg cccaagggc gcgtggtgc 
      ttctcggtc gatggcacc cagatgctg gcaagtggt 
      ggctccgat tgcttggtg cggctgtgg aacccgttc 
      gttccccag aaccccatt gcagtgctg tatgggcac 
      catacggcc atttcctca atgattttg caggatgat 
      gcaaaattt ttgtatagt ttgtctcag gataagagt 
      atcagggtt tgggacgtg gcgaagcaa acctgcgtc 
      cagaactat ctcgggata ccgcaggag ttgggggag 
      cacagcgac atgaccacg ctttacaat ccagagagc 
      cggcaatgg attatcggg agtaccatg ctggccgtt 
      atcccactc agtcccaag caaagtggg gaacacacc 
      gatgggttc acacacact gctggggtt tcggtggta 
      ctgtataac ccacttttc aagataatc gtcacttgc 
      ggcctggac agccacatc atcgtgtgg aatccctgg 
      gatggccgt cgcatgatc gccatcaaa gaagcccac 
      acgaaaagc gtccacgcc gagataatc cctattgag 
      ataacggcc gccagtttt gaccccgga tttcagcgt 
      ctagtaaca ggtgcccac gacggttcc ctaaaaatt 
      tggaacttc aacactgga acctgcctt cgcaacatg 
      aaaattgac caaaacgcc gaaatcaaa tgcatcatt 
      tgggtgaaa caccgcatt ctggtcatg ggctggaac 
      cgaagggtg acagagttc ggggacagt ggcgaagtt 
      gtggccgtg ggaggacag tttcacaaa aactgggac 
      ttgaggcat aaggaggac atcagctct gctgccgta 
      aggatacct cagactata gtcacaggg acctacgac 
      ggcgagctg atcatgtgg cggcttgag acagggcag 
      ccctacaag cagtttaat gccgccgat cccaccacc 
      aggattaaa attcagtac agaaaactg aagcacaaa 
      gtggaggca aaagtgaac aaattcagc aagaagaac 
      atcatcgcg cgagcgttg ggcaaaccc atcgagaag 
      gagaagaag gaaccgccg aaaccgcct cccgccgaa 
      gaggaaatc gtccgacct aacgtctcg cgccgcctc 
      tccgccgtg cagaagctc atcgggcag agctcgacg 
      tcgagccgg atcccttca agggcccgc cgcgtctcg 
      accgtggtg atgcccgag cagtgcatg cctctgaga 
      cgcctcgcc atccactcg atgctgttc ctcaacagc 
      cggccgatg gaccccgag gtcgggacg ctcctcatc 
      tcgctggag aacggggcc atccaggtg tggtcccac 
      cacatgagc ggcggcttc ataacgtcc ttctcggcc 
      atacacaag gccggggac tacgtcatt tgcatggcc 
      accgacgag aaaaacgac tacctcttc acgggctcc 
      agtgtgggt tacatcaaa acgtggctt ttgcgcaac 
      tactgcgag cccgaaacg gaaacgata agcatgccg 
      aaactgcga ctgcagttc ccgtttctg tggggcgac 
      ttcttcgcc ggccgcgcc gccaggatc gcaaatacc 
      caaagcctg cccatgctt ttgaacagc tacaaaggg 
      cacgttatg acgatttcg catttgacg tacattgac 
      gacgcccaa attttgata agtgccagt gctgattac 
      acggcgcgg atgtggacg ctgagcggt cgctatttg 
      caaactttg gggacgttt aagaagtgg aaaactgtt 
      cctcaaggc cacgaagcg cggcctgag gagttccag 
      ttcacgact cctccagac atcgaaaga gttggaagt 
      tcgacgact ctgcgagtg cttcgaggt ggtgccact 
      ctgcgtaag atgacgttc aagcaactg caagccaag 
      gaagtgaag gagttaacg catgtggac cacagcaag 
      atttacggc aacaggttg gaggaaccg atcttgggg 
      cattactac aagccctca gttaggacg acgcagttt 
      cacgaaatt aagttcgat acgtcgttt ccttatatt 
      cctgtttat caacattta atcatgccc ccgtcgcat 
      gatctgact ctgactagt ctcaagcca aagtcacaa 
      gaggcgtta caggaagtg aagaaaacc ccgaaagaa 
      gaagcggaa aagaaggat aaggaaagc aaacgctcc 
      tctgcttcg aaataattg cctctacta taaattaat 
      tatacgaaa ataaattct caaacaaat g
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