Status
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Gene information
General information
TC011954 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10983913..10987403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011954
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07648

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011954 RA
      MDVMVENYN QLFQKFSFE SASSITMER VKLWKEILW 
      KIKLKICYF IQELINEIA YLFIYYGEI FLTLTLRIS 
      TAYNNLFSL DSKYGLKKN TSICNNTCP YLFFPLRKI 
      SVTRLLQII SNNRAELCC HKLIDCLLA TYKSYDNSD 
      DDNSSDNSS LEIYRALTK HMSPPDPET PTKTKIINL 
      ENDNENFIS IEELISYEE KNVLDLLDT ILKISPSML 
      GTDGVKKSK STGGSKIKS KAREKVLDY YEQILWGEV 
      GNFLEHIIL WWGSSPLSA RPPHSSQHL REWITQFIP 
      TAGIPPVVL SALASLADA LGVHVTLTS WDQHFRLAL 
      VASKAMGNP DTGKLFCAV LQDLVTLVN QCEVTPDWI 
      IGAPLEELS LVEQIPVLH RLDHSIHTT RLWAASETK 
      KIANNWNVD AFFMVTHAD IVSCLGQLS GLRLADHGG 
      EVEKGGLGV HVQVCALMR AKLVSEVNE NIKKLKETP 
      NECVKGLAS VCRTISLAN LQMIFPENA YWKRVGTQA 
      PEKPSPYVD KYLERILTP ILVATDDHV ISNMILQIM 
      CEAWLDHIY MNKIKFSQF GACQLLCDF GYVETWLMN 
      CSVMSQTMR KKMIKNEVL RRCEGVGRL LLRCPGEQI 
      KMVDKNKKT KPTNSPPAE KSEQMPAEM YVPNQEQWL 
      ELRAIKKRN LFPTSLCCG KF
    CDS sequence
    • >TC011954 RA
      atggatgtt atggttgaa aattacaat cagttattt 
      cagaaattt agtttcgaa agcgcttcc tcaattact 
      atggaacga gtgaaactg tggaaggaa attctatgg 
      aaaatcaaa ttaaaaatt tgctatttt attcaagag 
      cttatcaac gaaatcgca tatttattt atttactat 
      ggcgagatc ttcctaact ttaacgcta agaatttcc 
      accgcttac aataactta ttttcactc gatagtaaa 
      tacgggtta aagaaaaac accagtatt tgtaacaat 
      acgtgtcct tacctcttc ttccctttg cgaaaaatc 
      tcagtgacg cgtcttctt caaataatt tcgaacaat 
      cgagctgag ctgtgttgc cacaaatta atcgattgt 
      ttgctcgcg acttacaag tcttatgat aacagtgac 
      gatgataac agttcagat aattcaagt ttagaaatt 
      taccgggct ttaaccaag cacatgtcg cccccagac 
      cccgaaacc cccacaaaa acaaaaata atcaacctg 
      gagaacgac aatgagaat ttcataagt atcgaggag 
      ctgatttcg tacgaagaa aaaaacgta ctggaccta 
      ctagacacg attttgaaa atatccccc agtatgtta 
      ggcaccgac ggggtgaaa aaatccaaa agcacgggc 
      ggcagcaaa atcaagtct aaagcccgg gagaaggtt 
      ttagattat tacgagcag attttatgg ggcgaagtt 
      ggcaatttc ctcgaacat atcatcctg tggtggggc 
      tcgagcccc ctgtccgca cgcccccct cacagcagc 
      caacatttg cgcgagtgg atcacccag tttatcccc 
      accgctgga atcccgcca gttgtctta tccgcattg 
      gccagttta gcggacgcg ctgggcgtc cacgtgacc 
      ctgacgtca tgggaccag cacttccga ttggccctg 
      gtggcgtcc aaggccatg ggaaacccc gacactggc 
      aaacttttc tgtgcggtt ttgcaagat ttggtcact 
      ttggtcaac caatgcgag gtcacccct gattggata 
      atcggggcc cccttggag gagttgtct ctggtggag 
      caaatcccc gttttgcac cggctggac cactcgatt 
      cacacgaca cggctttgg gccgcgagc gagaccaaa 
      aaaattgca aataactgg aatgtggat gcgtttttt 
      atggtcact catgcggac attgtgagc tgtttgggg 
      cagttgagt gggctaagg ctggccgac catgggggc 
      gaggtcgag aaggggggg ctgggggtc catgtgcag 
      gtttgcgcc ttgatgagg gccaaactg gtctccgaa 
      gtcaacgaa aatatcaaa aaattgaaa gagaccccg 
      aatgagtgc gtcaaaggc ttggccagt gtgtgcagg 
      accatcagt ttggccaac ttgcagatg atttttcca 
      gaaaatgcc tactggaag agggtgggc acccaagcg 
      cccgaaaag ccaagcccc tatgttgat aaatatttg 
      gagcgaatc ctaacgcca attctagtt gcgacagac 
      gatcatgtg atcagtaac atgattctg caaatcatg 
      tgcgaagcc tggcttgat cacatatat atgaataaa 
      atcaaattt tcgcaattt ggcgcttgc cagttactt 
      tgcgatttc gggtatgtt gagacttgg ttgatgaat 
      tgttccgtc atgagtcag acgatgagg aaaaaaatg 
      atcaaaaat gaagtgctg aggaggtgc gaaggcgtt 
      gggaggttg ctactgcgg tgtccgggg gagcaaatt 
      aaaatggta gataaaaac aaaaaaaca aagccgaca 
      aattcgcca cctgccgaa aaaagcgag caaatgccg 
      gccgagatg tacgttcca aaccaggaa caatggctc 
      gaactgcgc gctatcaag aagagaaac ctcttcccg 
      acctcctta tgttgcggg aaattctaa
    mRNA sequence
    • >TC011954 RA
      tagagaccc atttcttgt gggtgcaac atatttctt 
      ctttagtgc ctaaataaa tttgcatac aggtcgtaa 
      aacatgatt ttgcgtgtt ttcaaatca aaacaaaat 
      gataaacaa aaatcaatt aaatttatt ttaataatg 
      tcgctacta atttaatta aataataac attttgtgc 
      aataagttt ggtttgtgg tacgtattg tgtaaatta 
      gtatcgatt ttgcaaatt tcctaattt tctctgatg 
      tcgagaact atacaaacc gtcacaaaa tggttgcct 
      ttagacgtt aaagggtct gaagacatt ttcgaagat 
      tgcaaaaat ttaaaaaaa acttcgacg aatttgttg 
      cttcgcatt tgtgtcctt ttggagaat ttgaagaaa 
      aggtttggg tcgaaactt catcatagt tgtatgcaa 
      aacgagtta aatatccaa gaattagaa aatattaat 
      tcaacaatg gatgttatg gttgaaaat tacaatcag 
      ttatttcag aaatttagt ttcgaaagc gcttcctca 
      attactatg gaacgagtg aaactgtgg aaggaaatt 
      ctatggaaa atcaaatta aaaatttgc tattttatt 
      caagagctt atcaacgaa atcgcatat ttatttatt 
      tactatggc gagatcttc ctaacttta acgctaaga 
      atttccacc gcttacaat aacttattt tcactcgat 
      agtaaatac gggttaaag aaaaacacc agtatttgt 
      aacaatacg tgtccttac ctcttcttc cctttgcga 
      aaaatctca gtgacgcgt cttcttcaa ataatttcg 
      aacaatcga gctgagctg tgttgccac aaattaatc 
      gattgtttg ctcgcgact tacaagtct tatgataac 
      agtgacgat gataacagt tcagataat tcaagttta 
      gaaatttac cgggcttta accaagcac atgtcgccc 
      ccagacccc gaaaccccc acaaaaaca aaaataatc 
      aacctggag aacgacaat gagaatttc ataagtatc 
      gaggagctg atttcgtac gaagaaaaa aacgtactg 
      gacctacta gacacgatt ttgaaaata tcccccagt 
      atgttaggc accgacggg gtgaaaaaa tccaaaagc 
      acgggcggc agcaaaatc aagtctaaa gcccgggag 
      aaggtttta gattattac gagcagatt ttatggggc 
      gaagttggc aatttcctc gaacatatc atcctgtgg 
      tggggctcg agccccctg tccgcacgc ccccctcac 
      agcagccaa catttgcgc gagtggatc acccagttt 
      atccccacc gctggaatc ccgccagtt gtcttatcc 
      gcattggcc agtttagcg gacgcgctg ggcgtccac 
      gtgaccctg acgtcatgg gaccagcac ttccgattg 
      gccctggtg gcgtccaag gccatggga aaccccgac 
      actggcaaa cttttctgt gcggttttg caagatttg 
      gtcactttg gtcaaccaa tgcgaggtc acccctgat 
      tggataatc ggggccccc ttggaggag ttgtctctg 
      gtggagcaa atccccgtt ttgcaccgg ctggaccac 
      tcgattcac acgacacgg ctttgggcc gcgagcgag 
      accaaaaaa attgcaaat aactggaat gtggatgcg 
      ttttttatg gtcactcat gcggacatt gtgagctgt 
      ttggggcag ttgagtggg ctaaggctg gccgaccat 
      gggggcgag gtcgagaag ggggggctg ggggtccat 
      gtgcaggtt tgcgccttg atgagggcc aaactggtc 
      tccgaagtc aacgaaaat atcaaaaaa ttgaaagag 
      accccgaat gagtgcgtc aaaggcttg gccagtgtg 
      tgcaggacc atcagtttg gccaacttg cagatgatt 
      tttccagaa aatgcctac tggaagagg gtgggcacc 
      caagcgccc gaaaagcca agcccctat gttgataaa 
      tatttggag cgaatccta acgccaatt ctagttgcg 
      acagacgat catgtgatc agtaacatg attctgcaa 
      atcatgtgc gaagcctgg cttgatcac atatatatg 
      aataaaatc aaattttcg caatttggc gcttgccag 
      ttactttgc gatttcggg tatgttgag acttggttg 
      atgaattgt tccgtcatg agtcagacg atgaggaaa 
      aaaatgatc aaaaatgaa gtgctgagg aggtgcgaa 
      ggcgttggg aggttgcta ctgcggtgt ccgggggag 
      caaattaaa atggtagat aaaaacaaa aaaacaaag 
      ccgacaaat tcgccacct gccgaaaaa agcgagcaa 
      atgccggcc gagatgtac gttccaaac caggaacaa 
      tggctcgaa ctgcgcgct atcaagaag agaaacctc 
      ttcccgacc tccttatgt tgcgggaaa ttctaaata 
      aaactcctt tgttttgtt attaaacac tttaatcat 
      ca
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