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Gene information
General information
TC004220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451172.1: 1185..24823
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004220
    iBeetle screen iB_07647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head dorsal not closed - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: At the boundary beetween head and thorax the cuticle is either constricted or ruptured.
    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    maxilla size broader - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus proximal division boundary fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Tibiotarsus and femur fused and shortened.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004220 RA
      MFNGAFSPS PGSGYIPEM EQIISQLER GTLVMKYSL 
      RKKAEHKTL AIRRETRQI VWTRPATNS KPVHDGAVD 
      LRKIKEIRL GKSSKDFDK WADDSKKIE NLKCFVVYY 
      GSEFKLRVL SIAALSEKE CELWVKGLR YLVKDTINV 
      PYPLQVQVW LRREFYAME NPTETINIK DLKAFLPRI 
      NYKIPTNKL RELFNEVDT RKRAEIGFD DFAVLYQKI 
      IFDESNGAE IFDKLALYS SNLKSVSLQ EVQSFLAKE 
      QNDDLSNDD RAVSRFICD FLKDPQREV QEPHFTISE 
      FLDYLFSKQ NDLWDPNKD SVHQDMSKP LSHYWIASS 
      HNTYLTGDQ LSSESSVEA YARCLRMGC RCIELDCWD 
      GPDGMPFIY HGHTFTTKI KFMDVIKTI KEHAFATSE 
      YPVILSIED NCSLPQQRK MATAMQEVF GESLLTHPV 
      EKNEKQLPS PYQLRRRII LKHKKLPEG QDESSSILI 
      RHEGSEMDL RSSFRNGIM YLEDPVDKE WNPHFFVLT 
      ENKLFYTDS YKPDQESER SEDEDDSAA AFHRSKSDV 
      PNEELHFSE KWFHGKLAN GREEAEQLL KAYSHLGDG 
      TFLVRASVT FVGEYCLSF WRNGQVNHC RIRSKPDKQ 
      HTRYYLTDA KYFDSLYSL ITHYRNCPL VTAEFSITL 
      QEPVPQPKK HETEEWYHK HTGKMQADE ILRRVKMEG 
      AFLVRPSEN DANCYTISF RADKKIKHC RIKLEGRLY 
      TIGNVEFES LVELINYYE NHPLYRRVK LSYPISEEA 
      VKRMTMEQD DNVGYNSSP SYMDPSAFT PPKITVKAL 
      YDYRAQQPD ELSFCKHAI ITNVTKPEN SPGWWRGDY 
      GGMKQHFFP ANYVMELDR MESQDGSDD GSGESMLQG 
      QLDMNGAVV DLIHHPERP GLEWVVKIV TSTACTPLE 
      VALQSREMA VEWMLAIKE VAQRACALE NHHREMERT 
      FRIAKEMSN LIIYCRSIT FNIERARQQ GFIFYEMSS 
      FPETKAEKL MCQQEKKFF VKYHQHQFS RVYPKGQRV 
      DSSNYNPIN MWNIGSQMV ALNYQTGDK PMQLNQAKF 
      RDNGNCGYL LKPDFMFRD DFDPADRNT LVGVEPWIV 
      TLRIIAARH LFRPKKGTA SPFVEVEVI GAPFDSGTK 
      LSTKPVTDN GFNPRWMEE VIEFPVYNP YFALLRFVV 
      QDEDVFGEP NFIGQATYP VTCLRTGYR SVWLKNAYS 
      EDLELASLL IHISIKRTS VSPS
    • >TC004220 RB
      MFNGAFSPS PGSGYIPEM EQIISQLER GTLVMKYSL 
      RKKAEHKTL AIRRETRQI VWTRPATNS KPVHDGAVD 
      LRKIKEIRL GKSSKDFDK WADDSKKIE NLKCFVVYY 
      GSEFKLRVL SIAALSEKE CELWVKGLR YLVKDTINV 
      PYPLQVQVW LRREFYAME NPTETINIK DLKAFLPRI 
      NYKIPTNKL RELFNEVDT RKRAEIGFD DFAVLYQKI 
      IFDESNGAE IFDKLALYS SNLKSVSLQ EVQSFLAKE 
      QNDDLSNDD RAVSRFICD FLKDPQREV QEPHFTISE 
      FLDYLFSKQ NDLWDPNKD SVHQDMSKP LSHYWIASS 
      HNTYLTGDQ LSSESSVEA YARCLRMGC RCIELDCWD 
      GPDGMPFIY HGHTFTTKI KFMDVIKTI KEHAFATSE 
      YPVILSIED NCSLPQQRK MATAMQEVF GESLLTHPV 
      EKNEKQLPS PYQLRRRII LKHKKLPEG QDESSSILI 
      RHEGSEMDL RSSFRNGIM YLEDPVDKE WNPHFFVLT 
      ENKLFYTDS YKPDQESER SEDEDDSAA AFHRSKSDV 
      PNEELHFSE KWFHGKLAN GREEAEQLL KAYSHLGDG 
      TFLVRASVT FVGEYCLSF WRNGQVNHC RIRSKPDKQ 
      HTRYYLTDA KYFDSLYSL ITHYRNCPL VTAEFSITL 
      QEPVPQPKK HETEEWYHK HTGKMQADE ILRRVKMEG 
      AFLVRPSEN DANCYTISF RADKKIKHC RIKLEGRLY 
      TIGNVEFES LVELINYYE NHPLYRRVK LSYPISEEA 
      VKRMTMI
    • >TC004220 RC
      MDPSAFTPP KITVKALYD YRAQQPDEL SFCKHAIIT 
      NVTKPENSP GWWRGDYGG MKQHFFPAN YVMELDRME 
      SQDGSDDGS GESMLQGQL DMNGAVVDL IHHPERPGL 
      EWVVKIVTS TACTPLEVA LQSREMAVE WMLAIKEVA 
      QRACALENH HREMERTFR IAKEMSNLI IYCRSITFN 
      IERARQQGF IFYEMSSFP ETKAEKLMC QQEKKFFVK 
      YHQHQFSRV YPKGQRVDS SNYNPINMW NIGSQMVAL 
      NYQTGDKPM QLNQAKFRD NGNCGYLLK PDFMFRDDF 
      DPADRNTLV GVEPWIVTL RIIAARHLF RPKKGTASP 
      FVEVEVIGA PFDSGTKLS TKPVTDNGF NPRWMEEVI 
      EFPVYNPYF ALLRFVVQD EDVFGEPNF IGQATYPVT 
      CLRTGYRSV WLKNAYSED LELASLLIH ISIKRTSVS 
      PS
    CDS sequence
    • >TC004220 RA
      atgtttaat ggggctttt tcgccctct ccgggctcg 
      ggctacatc ccggagatg gagcagatt atcagccag 
      ctggagagg ggcaccctt gtcatgaag tactcgctg 
      aggaagaag gcagaacac aagacgttg gctatcagg 
      agggaaacg agacagatt gtgtggaca agacctgcg 
      acgaattcg aagccggtg catgatggg gctgttgat 
      ctcaggaaa attaaggaa attagactg gggaaaagc 
      tccaaggat tttgataag tgggccgat gattccaag 
      aaaatcgaa aatctcaag tgttttgtc gtttattat 
      ggatcggaa tttaagctg agggtcctc agtattgcg 
      gctctatcg gaaaaagag tgcgaattg tgggtgaaa 
      ggcttgcgc tacttggtc aaggataca atcaacgtt 
      ccctaccct ctccaggtg caagtttgg ctcaggcgt 
      gagttctac gccatggaa aaccccacc gaaaccata 
      aatattaaa gacttgaaa gcgttccta cctcgaatc 
      aactacaaa atccccaca aacaaactc cgcgaatta 
      ttcaacgaa gtcgataca cgcaaacgt gccgaaatc 
      ggctttgac gacttcgct gttctctac cagaaaatc 
      atttttgac gaaagtaac ggcgccgaa attttcgat 
      aaactcgca ctgtattcg tcgaatttg aaaagtgtg 
      agcttgcag gaagttcag agttttcta gccaaggaa 
      cagaacgat gatttgagt aatgacgat cgggccgtt 
      tcgcgattt atctgtgat tttttgaaa gaccctcaa 
      agggaggta caagaacct catttcaca attagtgaa 
      tttctcgat tatttattt tcgaaacag aatgatctg 
      tgggaccct aacaaggat tcagtccat caggacatg 
      tcaaagcct ttgtcacac tattggatc gcgtcttca 
      cataataca tatctaaca ggtgatcaa ttatcaagt 
      gagtcttcc gttgaagcg tacgcccgt tgtttgcgt 
      atgggttgt cgttgtatc gaactggat tgttgggat 
      ggtccagat gggatgccg ttcatttat catgggcat 
      acgtttacc acaaaaatt aaatttatg gatgtgatt 
      aaaacgata aaggagcat gcgtttgcc acatccgag 
      tatccagtc attttatca attgaagat aactgttcg 
      ttgccgcaa caaaggaaa atggcaaca gcaatgcag 
      gaagtcttc ggcgagtct ttgctcacc caccccgtg 
      gaaaaaaac gagaaacaa ctcccttcc ccctaccaa 
      ctccgtcgt cgaatcatc ctcaagcac aaaaaactc 
      cccgaaggc caagacgaa tcctcctcg atcctcatc 
      cgtcacgaa ggcagcgaa atggacttg cgcagttcg 
      tttcgtaac ggcatcatg tacctggag gaccccgtc 
      gacaaggaa tggaatccc cactttttc gtcctcacc 
      gaaaacaag ctcttctac acggacagt tacaagccc 
      gaccaggaa tcggaacgc agcgaagac gaagacgac 
      agtgctgcc gccttccat cgctcgaaa tcggatgtt 
      cccaacgaa gagttgcat tttagcgag aagtggttt 
      catggcaag ttggcgaat ggacgcgag gaggccgag 
      cagctgctc aaagcctac tcgcatttg ggggatggg 
      acgtttcta gtgagggca agtgtgacg tttgttggg 
      gagtattgt ttgtcgttt tggcggaat ggacaagtg 
      aatcattgt cggattagg tcgaagccg gataaacaa 
      cacactaga tattatcta accgatgcg aaatatttc 
      gatagtttg tacagtttg attacgcat tataggaat 
      tgtcctcta gtcactgct gaattttcc attactttg 
      caagagccg gtgccccag ccgaagaaa catgagacg 
      gaagaatgg tatcataaa catacgggg aagatgcaa 
      gctgatgaa attttgagg agggtgaag atggaaggg 
      gcgtttttg gttaggccg agtgaaaat gatgccaat 
      tgctacacg attagtttt agagcggat aaaaaaatc 
      aaacactgt cgaatcaaa ctggaaggt cgtctctac 
      accattggc aacgtcgaa ttcgaaagt ttagtcgaa 
      ttaatcaac tattacgaa aatcaccca ctttatcga 
      cgcgtcaaa ttgagttat ccaatcagc gaggaggcc 
      gtcaaaaga atgactatg gagcaagac gacaacgtc 
      ggctacaac agtagcccc tcctacatg gaccccagt 
      gcatttaca cccccaaaa atcaccgtc aaagccctc 
      tacgattat cgggcccaa caacccgac gaactctct 
      ttttgtaaa catgcaatc ataacaaac gtcactaaa 
      ccagaaaac agtcccggt tggtggcga ggcgattac 
      ggaggcatg aaacaacat tttttccca gccaactac 
      gtcatggaa ttggacaga atggagagt caagacggc 
      agtgacgac gggtctggt gaatcgatg cttcaaggc 
      cagctagat atgaatggt gccgtcgtc gatttgatt 
      catcatccg gaacgtcca ggtctcgaa tgggtggtg 
      aaaattgtc acttcgaca gcttgcacc cccttagag 
      gtggcgcta caatcgcga gaaatggca gtggaatgg 
      atgttggcc ataaaagag gtggctcaa agagcctgc 
      gccctcgaa aaccaccac cgcgagatg gagcgcact 
      tttcgtata gccaaagaa atgtccaat ttgataatt 
      tactgtcgt tcgatcacg ttcaacatt gaacgcgcc 
      cgccaacaa ggtttcatt ttctacgaa atgtcctcg 
      tttccggag acaaaagcc gaaaaactc atgtgtcaa 
      caagaaaaa aagtttttc gtgaaatac caccagcac 
      caattctca cgagtttat ccgaaaggt caacgagtc 
      gattcgtcc aattacaat ccgattaat atgtggaat 
      attggatca caaatggtc gctttgaat taccaaact 
      ggggataaa ccaatgcaa ttaaatcaa gccaaattt 
      agagataat gggaattgt ggctacttg ttgaagcct 
      gattttatg tttcgggat gattttgat ccagctgat 
      aggaataca ttggtgggg gtggagccc tggattgta 
      actttgagg ataatagca gccaggcat ttgtttagg 
      ccgaaaaag ggaacagcg agtccgttt gttgaggtt 
      gaagtgatt ggagcgccg tttgattcg gggacgaaa 
      ctatccacc aaaccagtt actgataac gggttcaac 
      ccgcgatgg atggaagaa gtgatcgaa ttccccgtc 
      tacaaccct tactttgct ctcttgagg ttcgtggtt 
      caggacgag gacgttttc ggcgaacct aactttata 
      gggcaagct acttaccct gtaacttgt ctcagaact 
      ggttatcgc agcgtttgg ctcaaaaac gcctacagt 
      gaagacctc gaattggcc tctctctta atccacatc 
      agtatcaag cgaacttcc gtgagtccg tcataa
    • >TC004220 RB
      atgtttaat ggggctttt tcgccctct ccgggctcg 
      ggctacatc ccggagatg gagcagatt atcagccag 
      ctggagagg ggcaccctt gtcatgaag tactcgctg 
      aggaagaag gcagaacac aagacgttg gctatcagg 
      agggaaacg agacagatt gtgtggaca agacctgcg 
      acgaattcg aagccggtg catgatggg gctgttgat 
      ctcaggaaa attaaggaa attagactg gggaaaagc 
      tccaaggat tttgataag tgggccgat gattccaag 
      aaaatcgaa aatctcaag tgttttgtc gtttattat 
      ggatcggaa tttaagctg agggtcctc agtattgcg 
      gctctatcg gaaaaagag tgcgaattg tgggtgaaa 
      ggcttgcgc tacttggtc aaggataca atcaacgtt 
      ccctaccct ctccaggtg caagtttgg ctcaggcgt 
      gagttctac gccatggaa aaccccacc gaaaccata 
      aatattaaa gacttgaaa gcgttccta cctcgaatc 
      aactacaaa atccccaca aacaaactc cgcgaatta 
      ttcaacgaa gtcgataca cgcaaacgt gccgaaatc 
      ggctttgac gacttcgct gttctctac cagaaaatc 
      atttttgac gaaagtaac ggcgccgaa attttcgat 
      aaactcgca ctgtattcg tcgaatttg aaaagtgtg 
      agcttgcag gaagttcag agttttcta gccaaggaa 
      cagaacgat gatttgagt aatgacgat cgggccgtt 
      tcgcgattt atctgtgat tttttgaaa gaccctcaa 
      agggaggta caagaacct catttcaca attagtgaa 
      tttctcgat tatttattt tcgaaacag aatgatctg 
      tgggaccct aacaaggat tcagtccat caggacatg 
      tcaaagcct ttgtcacac tattggatc gcgtcttca 
      cataataca tatctaaca ggtgatcaa ttatcaagt 
      gagtcttcc gttgaagcg tacgcccgt tgtttgcgt 
      atgggttgt cgttgtatc gaactggat tgttgggat 
      ggtccagat gggatgccg ttcatttat catgggcat 
      acgtttacc acaaaaatt aaatttatg gatgtgatt 
      aaaacgata aaggagcat gcgtttgcc acatccgag 
      tatccagtc attttatca attgaagat aactgttcg 
      ttgccgcaa caaaggaaa atggcaaca gcaatgcag 
      gaagtcttc ggcgagtct ttgctcacc caccccgtg 
      gaaaaaaac gagaaacaa ctcccttcc ccctaccaa 
      ctccgtcgt cgaatcatc ctcaagcac aaaaaactc 
      cccgaaggc caagacgaa tcctcctcg atcctcatc 
      cgtcacgaa ggcagcgaa atggacttg cgcagttcg 
      tttcgtaac ggcatcatg tacctggag gaccccgtc 
      gacaaggaa tggaatccc cactttttc gtcctcacc 
      gaaaacaag ctcttctac acggacagt tacaagccc 
      gaccaggaa tcggaacgc agcgaagac gaagacgac 
      agtgctgcc gccttccat cgctcgaaa tcggatgtt 
      cccaacgaa gagttgcat tttagcgag aagtggttt 
      catggcaag ttggcgaat ggacgcgag gaggccgag 
      cagctgctc aaagcctac tcgcatttg ggggatggg 
      acgtttcta gtgagggca agtgtgacg tttgttggg 
      gagtattgt ttgtcgttt tggcggaat ggacaagtg 
      aatcattgt cggattagg tcgaagccg gataaacaa 
      cacactaga tattatcta accgatgcg aaatatttc 
      gatagtttg tacagtttg attacgcat tataggaat 
      tgtcctcta gtcactgct gaattttcc attactttg 
      caagagccg gtgccccag ccgaagaaa catgagacg 
      gaagaatgg tatcataaa catacgggg aagatgcaa 
      gctgatgaa attttgagg agggtgaag atggaaggg 
      gcgtttttg gttaggccg agtgaaaat gatgccaat 
      tgctacacg attagtttt agagcggat aaaaaaatc 
      aaacactgt cgaatcaaa ctggaaggt cgtctctac 
      accattggc aacgtcgaa ttcgaaagt ttagtcgaa 
      ttaatcaac tattacgaa aatcaccca ctttatcga 
      cgcgtcaaa ttgagttat ccaatcagc gaggaggcc 
      gtcaaaaga atgactatg atatag
    • >TC004220 RC
      atggacccc agtgcattt acaccccca aaaatcacc 
      gtcaaagcc ctctacgat tatcgggcc caacaaccc 
      gacgaactc tctttttgt aaacatgca atcataaca 
      aacgtcact aaaccagaa aacagtccc ggttggtgg 
      cgaggcgat tacggaggc atgaaacaa cattttttc 
      ccagccaac tacgtcatg gaattggac agaatggag 
      agtcaagac ggcagtgac gacgggtct ggtgaatcg 
      atgcttcaa ggccagcta gatatgaat ggtgccgtc 
      gtcgatttg attcatcat ccggaacgt ccaggtctc 
      gaatgggtg gtgaaaatt gtcacttcg acagcttgc 
      accccctta gaggtggcg ctacaatcg cgagaaatg 
      gcagtggaa tggatgttg gccataaaa gaggtggct 
      caaagagcc tgcgccctc gaaaaccac caccgcgag 
      atggagcgc acttttcgt atagccaaa gaaatgtcc 
      aatttgata atttactgt cgttcgatc acgttcaac 
      attgaacgc gcccgccaa caaggtttc attttctac 
      gaaatgtcc tcgtttccg gagacaaaa gccgaaaaa 
      ctcatgtgt caacaagaa aaaaagttt ttcgtgaaa 
      taccaccag caccaattc tcacgagtt tatccgaaa 
      ggtcaacga gtcgattcg tccaattac aatccgatt 
      aatatgtgg aatattgga tcacaaatg gtcgctttg 
      aattaccaa actggggat aaaccaatg caattaaat 
      caagccaaa tttagagat aatgggaat tgtggctac 
      ttgttgaag cctgatttt atgtttcgg gatgatttt 
      gatccagct gataggaat acattggtg ggggtggag 
      ccctggatt gtaactttg aggataata gcagccagg 
      catttgttt aggccgaaa aagggaaca gcgagtccg 
      tttgttgag gttgaagtg attggagcg ccgtttgat 
      tcggggacg aaactatcc accaaacca gttactgat 
      aacgggttc aacccgcga tggatggaa gaagtgatc 
      gaattcccc gtctacaac ccttacttt gctctcttg 
      aggttcgtg gttcaggac gaggacgtt ttcggcgaa 
      cctaacttt atagggcaa gctacttac cctgtaact 
      tgtctcaga actggttat cgcagcgtt tggctcaaa 
      aacgcctac agtgaagac ctcgaattg gcctctctc 
      ttaatccac atcagtatc aagcgaact tccgtgagt 
      ccgtcataa
    mRNA sequence
    • >TC004220 RA
      gaccttgtc tcttgtcaa actcctgtc atctgtcaa 
      tttctaagt gcatttatt atcaatgcg gattaaatc 
      agtcaaaca aattaattc acgttatca gccaatgtg 
      acaatgtta gatatccaa acacgtgaa agtgcaaca 
      tcctgttga cgacccaac catccaccc actcaccaa 
      cggcatgcc ttgccacaa ataagcccc acttcctgt 
      tctctcagc agctttgac caggagcgg atcagggtg 
      atgttcggg atgtttaat ggggctttt tcgccctct 
      ccgggctcg ggctacatc ccggagatg gagcagatt 
      atcagccag ctggagagg ggcaccctt gtcatgaag 
      tactcgctg aggaagaag gcagaacac aagacgttg 
      gctatcagg agggaaacg agacagatt gtgtggaca 
      agacctgcg acgaattcg aagccggtg catgatggg 
      gctgttgat ctcaggaaa attaaggaa attagactg 
      gggaaaagc tccaaggat tttgataag tgggccgat 
      gattccaag aaaatcgaa aatctcaag tgttttgtc 
      gtttattat ggatcggaa tttaagctg agggtcctc 
      agtattgcg gctctatcg gaaaaagag tgcgaattg 
      tgggtgaaa ggcttgcgc tacttggtc aaggataca 
      atcaacgtt ccctaccct ctccaggtg caagtttgg 
      ctcaggcgt gagttctac gccatggaa aaccccacc 
      gaaaccata aatattaaa gacttgaaa gcgttccta 
      cctcgaatc aactacaaa atccccaca aacaaactc 
      cgcgaatta ttcaacgaa gtcgataca cgcaaacgt 
      gccgaaatc ggctttgac gacttcgct gttctctac 
      cagaaaatc atttttgac gaaagtaac ggcgccgaa 
      attttcgat aaactcgca ctgtattcg tcgaatttg 
      aaaagtgtg agcttgcag gaagttcag agttttcta 
      gccaaggaa cagaacgat gatttgagt aatgacgat 
      cgggccgtt tcgcgattt atctgtgat tttttgaaa 
      gaccctcaa agggaggta caagaacct catttcaca 
      attagtgaa tttctcgat tatttattt tcgaaacag 
      aatgatctg tgggaccct aacaaggat tcagtccat 
      caggacatg tcaaagcct ttgtcacac tattggatc 
      gcgtcttca cataataca tatctaaca ggtgatcaa 
      ttatcaagt gagtcttcc gttgaagcg tacgcccgt 
      tgtttgcgt atgggttgt cgttgtatc gaactggat 
      tgttgggat ggtccagat gggatgccg ttcatttat 
      catgggcat acgtttacc acaaaaatt aaatttatg 
      gatgtgatt aaaacgata aaggagcat gcgtttgcc 
      acatccgag tatccagtc attttatca attgaagat 
      aactgttcg ttgccgcaa caaaggaaa atggcaaca 
      gcaatgcag gaagtcttc ggcgagtct ttgctcacc 
      caccccgtg gaaaaaaac gagaaacaa ctcccttcc 
      ccctaccaa ctccgtcgt cgaatcatc ctcaagcac 
      aaaaaactc cccgaaggc caagacgaa tcctcctcg 
      atcctcatc cgtcacgaa ggcagcgaa atggacttg 
      cgcagttcg tttcgtaac ggcatcatg tacctggag 
      gaccccgtc gacaaggaa tggaatccc cactttttc 
      gtcctcacc gaaaacaag ctcttctac acggacagt 
      tacaagccc gaccaggaa tcggaacgc agcgaagac 
      gaagacgac agtgctgcc gccttccat cgctcgaaa 
      tcggatgtt cccaacgaa gagttgcat tttagcgag 
      aagtggttt catggcaag ttggcgaat ggacgcgag 
      gaggccgag cagctgctc aaagcctac tcgcatttg 
      ggggatggg acgtttcta gtgagggca agtgtgacg 
      tttgttggg gagtattgt ttgtcgttt tggcggaat 
      ggacaagtg aatcattgt cggattagg tcgaagccg 
      gataaacaa cacactaga tattatcta accgatgcg 
      aaatatttc gatagtttg tacagtttg attacgcat 
      tataggaat tgtcctcta gtcactgct gaattttcc 
      attactttg caagagccg gtgccccag ccgaagaaa 
      catgagacg gaagaatgg tatcataaa catacgggg 
      aagatgcaa gctgatgaa attttgagg agggtgaag 
      atggaaggg gcgtttttg gttaggccg agtgaaaat 
      gatgccaat tgctacacg attagtttt agagcggat 
      aaaaaaatc aaacactgt cgaatcaaa ctggaaggt 
      cgtctctac accattggc aacgtcgaa ttcgaaagt 
      ttagtcgaa ttaatcaac tattacgaa aatcaccca 
      ctttatcga cgcgtcaaa ttgagttat ccaatcagc 
      gaggaggcc gtcaaaaga atgactatg gagcaagac 
      gacaacgtc ggctacaac agtagcccc tcctacatg 
      gaccccagt gcatttaca cccccaaaa atcaccgtc 
      aaagccctc tacgattat cgggcccaa caacccgac 
      gaactctct ttttgtaaa catgcaatc ataacaaac 
      gtcactaaa ccagaaaac agtcccggt tggtggcga 
      ggcgattac ggaggcatg aaacaacat tttttccca 
      gccaactac gtcatggaa ttggacaga atggagagt 
      caagacggc agtgacgac gggtctggt gaatcgatg 
      cttcaaggc cagctagat atgaatggt gccgtcgtc 
      gatttgatt catcatccg gaacgtcca ggtctcgaa 
      tgggtggtg aaaattgtc acttcgaca gcttgcacc 
      cccttagag gtggcgcta caatcgcga gaaatggca 
      gtggaatgg atgttggcc ataaaagag gtggctcaa 
      agagcctgc gccctcgaa aaccaccac cgcgagatg 
      gagcgcact tttcgtata gccaaagaa atgtccaat 
      ttgataatt tactgtcgt tcgatcacg ttcaacatt 
      gaacgcgcc cgccaacaa ggtttcatt ttctacgaa 
      atgtcctcg tttccggag acaaaagcc gaaaaactc 
      atgtgtcaa caagaaaaa aagtttttc gtgaaatac 
      caccagcac caattctca cgagtttat ccgaaaggt 
      caacgagtc gattcgtcc aattacaat ccgattaat 
      atgtggaat attggatca caaatggtc gctttgaat 
      taccaaact ggggataaa ccaatgcaa ttaaatcaa 
      gccaaattt agagataat gggaattgt ggctacttg 
      ttgaagcct gattttatg tttcgggat gattttgat 
      ccagctgat aggaataca ttggtgggg gtggagccc 
      tggattgta actttgagg ataatagca gccaggcat 
      ttgtttagg ccgaaaaag ggaacagcg agtccgttt 
      gttgaggtt gaagtgatt ggagcgccg tttgattcg 
      gggacgaaa ctatccacc aaaccagtt actgataac 
      gggttcaac ccgcgatgg atggaagaa gtgatcgaa 
      ttccccgtc tacaaccct tactttgct ctcttgagg 
      ttcgtggtt caggacgag gacgttttc ggcgaacct 
      aactttata gggcaagct acttaccct gtaacttgt 
      ctcagaact ggttatcgc agcgtttgg ctcaaaaac 
      gcctacagt gaagacctc gaattggcc tctctctta 
      atccacatc agtatcaag cgaacttcc gtgagtccg 
      tcataagca taagtaaag cgaacgctt tgaaaacag 
      aacatgaca atatcagac acttgttaa aagacttcc 
      acaattttt cgatcgcct catacatat catcgagat 
      tatgtacct tccaagtca tatcatttt taggacaga 
      gatgtattt tttgtatga gatgaatct attttcgga 
      tatgcggcc tcgttttgt ttttacgga ggattaaaa 
      acttagcga aaat
    • >TC004220 RB
      gaccttgtc tcttgtcaa actcctgtc atctgtcaa 
      tttctaagt gcatttatt atcaatgcg gattaaatc 
      agtcaaaca aattaattc acgttatca gccaatgtg 
      acaatgtta gatatccaa acacgtgaa agtgcaaca 
      tcctgttga cgacccaac catccaccc actcaccaa 
      cggcatgcc ttgccacaa ataagcccc acttcctgt 
      tctctcagc agctttgac caggagcgg atcagggtg 
      atgttcggg atgtttaat ggggctttt tcgccctct 
      ccgggctcg ggctacatc ccggagatg gagcagatt 
      atcagccag ctggagagg ggcaccctt gtcatgaag 
      tactcgctg aggaagaag gcagaacac aagacgttg 
      gctatcagg agggaaacg agacagatt gtgtggaca 
      agacctgcg acgaattcg aagccggtg catgatggg 
      gctgttgat ctcaggaaa attaaggaa attagactg 
      gggaaaagc tccaaggat tttgataag tgggccgat 
      gattccaag aaaatcgaa aatctcaag tgttttgtc 
      gtttattat ggatcggaa tttaagctg agggtcctc 
      agtattgcg gctctatcg gaaaaagag tgcgaattg 
      tgggtgaaa ggcttgcgc tacttggtc aaggataca 
      atcaacgtt ccctaccct ctccaggtg caagtttgg 
      ctcaggcgt gagttctac gccatggaa aaccccacc 
      gaaaccata aatattaaa gacttgaaa gcgttccta 
      cctcgaatc aactacaaa atccccaca aacaaactc 
      cgcgaatta ttcaacgaa gtcgataca cgcaaacgt 
      gccgaaatc ggctttgac gacttcgct gttctctac 
      cagaaaatc atttttgac gaaagtaac ggcgccgaa 
      attttcgat aaactcgca ctgtattcg tcgaatttg 
      aaaagtgtg agcttgcag gaagttcag agttttcta 
      gccaaggaa cagaacgat gatttgagt aatgacgat 
      cgggccgtt tcgcgattt atctgtgat tttttgaaa 
      gaccctcaa agggaggta caagaacct catttcaca 
      attagtgaa tttctcgat tatttattt tcgaaacag 
      aatgatctg tgggaccct aacaaggat tcagtccat 
      caggacatg tcaaagcct ttgtcacac tattggatc 
      gcgtcttca cataataca tatctaaca ggtgatcaa 
      ttatcaagt gagtcttcc gttgaagcg tacgcccgt 
      tgtttgcgt atgggttgt cgttgtatc gaactggat 
      tgttgggat ggtccagat gggatgccg ttcatttat 
      catgggcat acgtttacc acaaaaatt aaatttatg 
      gatgtgatt aaaacgata aaggagcat gcgtttgcc 
      acatccgag tatccagtc attttatca attgaagat 
      aactgttcg ttgccgcaa caaaggaaa atggcaaca 
      gcaatgcag gaagtcttc ggcgagtct ttgctcacc 
      caccccgtg gaaaaaaac gagaaacaa ctcccttcc 
      ccctaccaa ctccgtcgt cgaatcatc ctcaagcac 
      aaaaaactc cccgaaggc caagacgaa tcctcctcg 
      atcctcatc cgtcacgaa ggcagcgaa atggacttg 
      cgcagttcg tttcgtaac ggcatcatg tacctggag 
      gaccccgtc gacaaggaa tggaatccc cactttttc 
      gtcctcacc gaaaacaag ctcttctac acggacagt 
      tacaagccc gaccaggaa tcggaacgc agcgaagac 
      gaagacgac agtgctgcc gccttccat cgctcgaaa 
      tcggatgtt cccaacgaa gagttgcat tttagcgag 
      aagtggttt catggcaag ttggcgaat ggacgcgag 
      gaggccgag cagctgctc aaagcctac tcgcatttg 
      ggggatggg acgtttcta gtgagggca agtgtgacg 
      tttgttggg gagtattgt ttgtcgttt tggcggaat 
      ggacaagtg aatcattgt cggattagg tcgaagccg 
      gataaacaa cacactaga tattatcta accgatgcg 
      aaatatttc gatagtttg tacagtttg attacgcat 
      tataggaat tgtcctcta gtcactgct gaattttcc 
      attactttg caagagccg gtgccccag ccgaagaaa 
      catgagacg gaagaatgg tatcataaa catacgggg 
      aagatgcaa gctgatgaa attttgagg agggtgaag 
      atggaaggg gcgtttttg gttaggccg agtgaaaat 
      gatgccaat tgctacacg attagtttt agagcggat 
      aaaaaaatc aaacactgt cgaatcaaa ctggaaggt 
      cgtctctac accattggc aacgtcgaa ttcgaaagt 
      ttagtcgaa ttaatcaac tattacgaa aatcaccca 
      ctttatcga cgcgtcaaa ttgagttat ccaatcagc 
      gaggaggcc gtcaaaaga atgactatg atatagtat 
      actttttta aactctaaa cctataaga acacgagta 
      acatacata atttttaag ccaataaaa taatgaatt 
      aaaa
    • >TC004220 RC
      gtcgtcttt aattatcgg attatttta aatgttttc 
      gagcaagac gacaacgtc ggctacaac agtagcccc 
      tcctacatg gaccccagt gcatttaca cccccaaaa 
      atcaccgtc aaagccctc tacgattat cgggcccaa 
      caacccgac gaactctct ttttgtaaa catgcaatc 
      ataacaaac gtcactaaa ccagaaaac agtcccggt 
      tggtggcga ggcgattac ggaggcatg aaacaacat 
      tttttccca gccaactac gtcatggaa ttggacaga 
      atggagagt caagacggc agtgacgac gggtctggt 
      gaatcgatg cttcaaggc cagctagat atgaatggt 
      gccgtcgtc gatttgatt catcatccg gaacgtcca 
      ggtctcgaa tgggtggtg aaaattgtc acttcgaca 
      gcttgcacc cccttagag gtggcgcta caatcgcga 
      gaaatggca gtggaatgg atgttggcc ataaaagag 
      gtggctcaa agagcctgc gccctcgaa aaccaccac 
      cgcgagatg gagcgcact tttcgtata gccaaagaa 
      atgtccaat ttgataatt tactgtcgt tcgatcacg 
      ttcaacatt gaacgcgcc cgccaacaa ggtttcatt 
      ttctacgaa atgtcctcg tttccggag acaaaagcc 
      gaaaaactc atgtgtcaa caagaaaaa aagtttttc 
      gtgaaatac caccagcac caattctca cgagtttat 
      ccgaaaggt caacgagtc gattcgtcc aattacaat 
      ccgattaat atgtggaat attggatca caaatggtc 
      gctttgaat taccaaact ggggataaa ccaatgcaa 
      ttaaatcaa gccaaattt agagataat gggaattgt 
      ggctacttg ttgaagcct gattttatg tttcgggat 
      gattttgat ccagctgat aggaataca ttggtgggg 
      gtggagccc tggattgta actttgagg ataatagca 
      gccaggcat ttgtttagg ccgaaaaag ggaacagcg 
      agtccgttt gttgaggtt gaagtgatt ggagcgccg 
      tttgattcg gggacgaaa ctatccacc aaaccagtt 
      actgataac gggttcaac ccgcgatgg atggaagaa 
      gtgatcgaa ttccccgtc tacaaccct tactttgct 
      ctcttgagg ttcgtggtt caggacgag gacgttttc 
      ggcgaacct aactttata gggcaagct acttaccct 
      gtaacttgt ctcagaact ggttatcgc agcgtttgg 
      ctcaaaaac gcctacagt gaagacctc gaattggcc 
      tctctctta atccacatc agtatcaag cgaacttcc 
      gtgagtccg tcataagca taagtaaag cgaacgctt 
      tgaaaacag aacatgaca atatcagac acttgttaa 
      aagacttcc acaattttt cgatcgcct catacatat 
      catcgagat tatgtacct tccaagtca tatcatttt 
      taggacaga gatgtattt tttgtatga gatgaatct 
      attttcgga tatgcggcc tcgttttgt ttttacgga 
      ggattaaaa acttagcga aaat
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