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Gene information
General information
TC012506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9548855..9552282
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012506
    iBeetle screen iB_07640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012506 RA
      MAGPGYVLG VDIGTTSVK VCVFNPENN EIVAQIGKE 
      TQSNVPSDQ GIDGNKQDV PKIISALNT CVAKLQKNL 
      LQQITKIGI CGQMHGVMF WNQDKSWER VEKDNNLVR 
      FDVVQDQVS ALYTWQDSR CDPSFLSSL PVPQSHLKV 
      YTGFGNATI FWMAKNKPE KLKRYNRCG TIQDFAVAM 
      LCNLSRPIM SDQNAASWG YFDCVHHRW NEEILKQAG 
      FPISLLPEI KTSGEIAGY LADNWHSIP KGTPIGIAL 
      ADLQCSVLS TIETNKDAV LNISTSAQI AFVAEDYKP 
      STGPPSVST VEYVPYFKN KYLAVAASL NGGNSLATF 
      VKMVQQWTM TLGFSVPQS KVWEKVISL GLEENSVSD 
      LKISPTCLG ERHAPNVTA SVTNINIGN LELGKVFRA 
      LCNGLLVNL HSMMPRDIL QNAKITRIV GNGSGLSRN 
      KVLQNEVLN LYKLPLVFT TGGDAAKGA AMAMNLDTV 
      NFK
    CDS sequence
    • >TC012506 RA
      atggccggc ccggggtac gtcctaggc gtcgacatc 
      ggcaccact tcggtcaaa gtgtgcgtt ttcaacccc 
      gaaaacaac gaaatcgtc gctcaaatc ggcaaggag 
      acacaatcg aacgtcccc agtgaccaa ggcatcgac 
      ggaaacaaa caagacgtc ccgaaaatc atttccgcc 
      ctcaacacg tgcgtggcc aaattacag aaaaatctg 
      ctgcagcag ataaccaaa atcgggata tgtgggcaa 
      atgcacgga gtcatgttc tggaaccag gataaatcc 
      tgggaacgg gtagagaaa gacaacaac ttagtgcgc 
      ttcgacgtt gtccaagat caagtgtca gctctctac 
      acttggcag gacagccgt tgtgaccct tccttcctg 
      tcatcccta cccgtcccc caatctcac ttgaaggtc 
      tacactggt ttcggcaac gccacaatc ttctggatg 
      gccaaaaat aaacctgaa aagctaaaa cggtataac 
      cgatgcgga actatccag gatttcgcc gttgctatg 
      ctttgcaac ttatcccga cctataatg tcggaccag 
      aatgcggcg agttgggga tactttgat tgcgtgcat 
      caccgctgg aatgaggaa atactgaaa caggcaggt 
      ttcccgata tccctcctt ccggaaatc aaaacaagc 
      ggagagata gcgggatac ctggcggac aactggcac 
      agtattccc aagggaact cccattgga atcgccctt 
      gcagacttg caatgttca gttttatcc accattgag 
      acaaataag gatgcagtt ttaaacata tcgacttcg 
      gcacaaatc gccttcgtc gccgaagac tacaaacca 
      agtacagga cctccgtct gtatcaacc gtagagtac 
      gtcccttac ttcaaaaac aagtatctg gctgttgcc 
      gcgtcccta aatgggggc aactccttg gcgactttt 
      gtcaaaatg gtccaacag tggactatg actttgggg 
      ttctcggtg ccacaatct aaggtttgg gagaaagtc 
      attagtttg ggtttggag gagaacagt gtttcagac 
      ttgaaaatt tcccccact tgtcttggt gaaagacac 
      gcccctaat gtcaccgcc tctgtcact aacattaat 
      atcgggaat ctcgaattg ggaaaagtc tttcgagct 
      ttatgcaat ggcctactt gttaatttg catagtatg 
      atgcctagg gatattctc caaaatgcg aagataaca 
      aggattgtt gggaatggg tcaggtctg tccaggaac 
      aaagtcttg caaaacgaa gtgttgaat ctttacaag 
      ttgcctttg gtctttact acggggggt gatgcggct 
      aaaggggcc gccatggcc atgaattta gataccgta 
      aattttaag taa
    mRNA sequence
    • >TC012506 RA
      tcgtacgtg aacagggtc acgccgaaa atgacgcaa 
      gttgtgtcg gcaatcaat agcagtcga ctgccgttt 
      tatccagcc gacgaatca aaatctctt cgtgaccat 
      ttctccaac gataaaaat aatatttgg cacttagta 
      caatagaat aatatggta caattatga atgggcgtt 
      tgttttaat aatagtcgt ggggacgcc ggctgaaga 
      ctgtgagcg ccatggccg gcccggggt acgtcctag 
      gcgtcgaca tcggcacca cttcggtca aagtgtgcg 
      ttttcaacc ccgaaaaca acgaaatcg tcgctcaaa 
      tcggcaagg agacacaat cgaacgtcc ccagtgacc 
      aaggcatcg acggaaaca aacaagacg tcccgaaaa 
      tcatttccg ccctcaaca cgtgcgtgg ccaaattac 
      agaaaaatc tgctgcagc agataacca aaatcggga 
      tatgtgggc aaatgcacg gagtcatgt tctggaacc 
      aggataaat cctgggaac gggtagaga aagacaaca 
      acttagtgc gcttcgacg ttgtccaag atcaagtgt 
      cagctctct acacttggc aggacagcc gttgtgacc 
      cttccttcc tgtcatccc tacccgtcc cccaatctc 
      acttgaagg tctacactg gtttcggca acgccacaa 
      tcttctgga tggccaaaa ataaacctg aaaagctaa 
      aacggtata accgatgcg gaactatcc aggatttcg 
      ccgttgcta tgctttgca acttatccc gacctataa 
      tgtcggacc agaatgcgg cgagttggg gatactttg 
      attgcgtgc atcaccgct ggaatgagg aaatactga 
      aacaggcag gtttcccga tatccctcc ttccggaaa 
      tcaaaacaa gcggagaga tagcgggat acctggcgg 
      acaactggc acagtattc ccaagggaa ctcccattg 
      gaatcgccc ttgcagact tgcaatgtt cagttttat 
      ccaccattg agacaaata aggatgcag ttttaaaca 
      tatcgactt cggcacaaa tcgccttcg tcgccgaag 
      actacaaac caagtacag gacctccgt ctgtatcaa 
      ccgtagagt acgtccctt acttcaaaa acaagtatc 
      tggctgttg ccgcgtccc taaatgggg gcaactcct 
      tggcgactt ttgtcaaaa tggtccaac agtggacta 
      tgactttgg ggttctcgg tgccacaat ctaaggttt 
      gggagaaag tcattagtt tgggtttgg aggagaaca 
      gtgtttcag acttgaaaa tttccccca cttgtcttg 
      gtgaaagac acgccccta atgtcaccg cctctgtca 
      ctaacatta atatcggga atctcgaat tgggaaaag 
      tctttcgag ctttatgca atggcctac ttgttaatt 
      tgcatagta tgatgccta gggatattc tccaaaatg 
      cgaagataa caaggattg ttgggaatg ggtcaggtc 
      tgtccagga acaaagtct tgcaaaacg aagtgttga 
      atctttaca agttgcctt tggtcttta ctacggggg 
      gtgatgcgg ctaaagggg ccgccatgg ccatgaatt 
      tagataccg taaatttta agtaactag tgtccaaat 
      atttatgtc tgatttaaa tttagagtg ataataacg 
      agtcccgta caatcgtcc aatagtggc ctattttgt 
      tatgaatgt aacacaaat aaagatata tctacact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY