| General information | |||
| TC031589
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450460.1: 88902..128058 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031589 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07638 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031589 RA
HTHWAKGNP VPIPEPGSG TVNNSGPRK RVRRGNPKG PGDAVGRSG KSFLFCMSV RVPWNPLAG RYGLEREEH RSCGGVRIF PSDLENPGE GHVEASRRF VPISAAGLQ GEEPLVDRI MIGSEDRGV SGLARKRVA ADVPGLCEV KRCSVSAVN VESEFGPVP WPPAEPSCC EAPGAPVRA SGSPLRPPF NGLESVILI RSHLKSYAE YVRVVRVGN CPRMVWDRL NLSRGSCRG LPPLHFIGM AFDTLNEAS DLEEHGSPP PRIRPGTNC LDNPIGPMG ADHAMDMDS EDEAGAHGP PADNAHLTS GEPIEIILM LPFQSRSCG ICLNAGKGN FRALSLDDE ERHLRERHP LSLILYKCS DCKGQYRSK RAALCHAPK CTGPTPDPQ GNALRCHLC GLVCKSQSG VTQHLRHRH PLVRNTQRA AEESARAER AALPRPLRR NTRPVFSEE DEAKMLELE DPAGAEDSV LPVVETDEP AEENIPLEY PELPGDEEG APACSQTIL NTEGPDGLG SPPVPVEEE MASSGSTSN NVDTGWRES IITAALGVE IPKAISQEP AAVIQELQD ALREAVIGV FPQDRLDEM YERVLKVVN PDDSQERPK RQRKKGKSR NAFRRYVYS QTQDLFKKN PGQLARYVR EDVRWLEQG RVQLQRDNI ERMYNKLWG TKPDVLPPH WDYPLPLDT ADLESHPGY KVGGELASV SCMAFADDI FLIAANVPQ TCTLLRVTE DYLERLGMR ISAPKCTSF EIRPTKDSW YVADPGLTL TKGERIPVA AVDAVFSYL GVEISPWAG ITSEGIERD WRGTLHRVQ RLALKPHQK LELISRYLV PHFLYKLVV TIPSITLIR QLDQELRVV VKQICHLPQ STADGMIYC RKVDGGLGI PKLEIVTVT SILKAGLKF RDSQDKIMQ ALWLASGMS SRLSTLAKA TRVQPWPPN NIKDLDRHK VARKREELA RWASLTSQG KSVKSFAGS RTANAWLIN KKLLKPSTF ISALRLRGN VAGDRVALN RAIPQANLM CRRCGSQRE TLGHILGIC TSTNALRIS RHDEIKNLI VDEAAKKDD EVAVTLEPT IRHPMRGNL KPDLVVQNR EGVYVVDVT VRHEDGNLL TQGRQDKLD KYEVLLPIL QERLGAPTG EVLPIVVGT RGAMPKETV EALKKLRIT DRQTLLTIS LIALRMSVK IYHTFMDYA NARPRPGGG ANYPHRLEK KIGDDEFPD IKREDNKAP RSYAIIVKG KDENENASK IKEKLLKVE TDIKIQRVR PSRVGGGLV IETTSEKDL HKVRQKIME QKEEWSIED AKRRGPSIM VYDVPVHID NEEFAKEVY KKNLATVLR EEEFVKDFR IVSKRSSEK EQSTGSGKE NVLICVSPQ ICMKVFVSN EVPPAAAVV MNCKGVDVV KMEIEGESR GVCVSVAGE FGDFCVAST YCRYGYDLR PYLEWITRV KEKCVNMPL LIGMDANAV SPLWYSKMH RRERGEREA RGERLEDLV AQEEFLILN QPSQKYTFS GPMGESDID VTMCNREWM YKYETSWDV NEEWGCSDH NCIVMCVNG REGGGRVRR AKKKRWRMN GVDWEKFKK KLNEVRDTA LYSGESIDG KIYLLEAWI ETAMRKATI KINVGSREV RWWTNELGS LKAEASKER KRWQRARKR AGSIGRSDP WREVYKICK GGMSDRGLS VVKRGGVLT KSWKETMEV LLDEFFPVD SVDENNGDE ESGVSGDER PREIEEWEL DASIQRMNG KKSPGMDEI TPEMVKAVW MCMKEELIE LYNECVRME YFPARWKEA RVVILLKGG DRDRTLPRS YRAICLLPV LGKVLERIM VERLHGRIE NIGSNRQYG FKKGMGTED AWMRAKNVV VNSEKKYVM GLFVDFKGA FDNLRWNVL LARIREVGC LEYGLWRSY FCNRKVCAD GEGGEIWKQ VWKGCPQGS ICGPSVWNM MMDELLNDL ESLNVEVVA YADDVLCLI EGMSRAEIE VKGTEVSDV IERWGRRVG VDVSEDKTV MMLMKGSLA DTRPANVRM KGKVVRTVK EVKYLGIHV GERMNFRPH VERLNAKVI AAMGRMRRV LRKEWGVGK KTTRNWYKG LMVACVAYG VGVWCDVLK YEYARVLVN RCQRVCLYG CLPVCKTVS TDAMQVLMG ELPWDLELL RRGVRYRIG RGAPFGEVS PVREVEVVE IGVNESKKL LEERLWKTW QERWEESEN GRITYRFIG RVDYVKKNE WFGPSMYGM FLITGHGSM NELLFGRNL SVTDRCKCG EAESWLHIL YDCRLYEDI REEYHATPC VNYRLDMTS IKDEVLQPH VDDLMSLLD ERFSTIDSI PEIENATKE LYSGLAEIF SNTGKNAKI LWEGPIGGT KNLRDFEKF REYSDHLFA EMTRAKQTF KEKVEKWRQ KCWLEFAEN DLARNLWGV VYKLASEKL KTRGILQSF QTDEDDNIT RDFQSTMEF LITNLLPDD DTDANTEEQ RVTQSDFRT TAAEVGSDA VITEEKVLT GHGNFGIHQ LRLGKSENS LCPNCPDFD DDPVHRILE CPLFGEIQE RIRGITGTW PPDLTQVPY IDNDEVFSA LSLDLTQPD VRTLHWSSH DMIRSFYGS SAPSPDKAK CDLCSQKMS YKSSTSNMK RHVQRKHPT VKMKPEVEK NKNISDKED IVRDREEIP STSSLDQEI QAHEQAETP NIKQPSKTR KSKQNHATP CVNYRLDMT SIKDEVLQP HVDDLMSLL DERFSTIDF IPEIENATK ELYSGLAEI FLKHWKKRK NFVERSDWA KQTLKEKVE KRRLKCWLE VAENDLARE PWGVVYKLA SEKFKTRGI LQSFQTGED NNITRDFQS TMEFLIMNL LPDDDLTPT PRSNVSLKA IFEQPLRKW GVLTGHGNF GIHQLRLGK SENSLCPNC PDYDDDPVH RILECPLFG EVQERIRGI TGTWPPDLT QVLYIDNDE MFSALSLDL TPPHYCRAA LQSERQAQL FKNGARVEA VKKEQVTKK TEKIKWKLK TDEADQFQI EEKLKKRIP LSLQPKLEE TLKELERQG IIEKVEYPT DWVNSLVIV EKKTGQLRL CLDPKDLNK AIKREHYMI PTAQDIIPR LAGKKIFTV IDMSAGFWH VPLDKESSD LCCFNTHDE DTSAILQKL DEMTTDTPP DHQWRENII YSPISSGLE LSAQEPANR GWIDNKPKG WTGRDFVRA IHLRTANLP SRGIPSSPL RNGNGSAGT GVTHGLRIL RHKAIAKTI AKHCQSNGW KVEGELHVR HPSGQLFKP DLVVHTSSE NMVTYADVH LLVVPAGNG HLSLMELSS LLKNGGRGL GIVVWFRCS VNGNLPTVP PGPQAGAPA FTADRNANG KPAQNPCPY CAQSFTTAN GRGLHIRRA HPDEANNAK DIERILQAP QINLPEMRD GDAPRQTDP QQENPPEPP SFDGAIRGA IADLVGGVD WQRLGFQGD RLCNIARRA CDGRDVSGQ LLGWLRDVF PVKRVSTRG DQSNLDVDG ALVSRHTAR TREYARAQE LYRKEPKAC LARILGDRR EGANRGPNR NLAFVDFWR GVFSEGSAE VEGWAEEVS DHGELARRV WDPISIEEV VRSRVRNSA APGPDGIAV SVWNKLPPE TAALLFNVL LLGRCLPAE LTRTPTVCI PKTDAPRTP ADYRPISIA SAGLPQPFV EYVRSIYRS AETVLEDGG RRHFVQVRC GVRQGDPLS PLLFNLVLD RALKRLSTE VDFRLTDAT KVPALAFAD DVVLCATIQ SGCSRSGAS HSRSLVEPQ QVSALSLVA SGRHHKVKL VTKPTFKVG QNTIHQVDA SSIWKYLGI QFRGSGMCG CGSEGVAAG LTRITCAPL KPQQRMHLL RVFFLPKFY HAWTFGRLN AGVLRRQDV VVRTSVRTW LRLPHDIPV GYFHAPTKS GGLRIPQLS RFIPFLRLK RFDRLGVSA VDYVRECAF TDIANRKIR WCRGRLLGI VDQVAGGRN ALDAYWTAQ LHQSVEGRA LSAVQCVHG LSDGSRTRR RKSSKISSD VPLVLPSVG ASALTVSSG FLLYFVNPT RSPLALLLE HLSFVQESA FQQNERLAN FSREDFSPR FKIGPKDRS TTTWVVEID RKIRTVILR DWLAIGWQT CKVSDHIRI TRCYKCQKF GHQSKDCQS EQTCGRCAG NYSFKECKV KEENSVCAN CKSSKLPEE EQKHQTDSP KCPTYIRKI KDLIGKTDY GS
- >TC031589 RA
cacacccat tgggcgaaa gggaatccg gttcctatt ccggaaccc ggcagcgga accgttaat aattcgggc cctcgcaag agagttcgt cggggcaac ccaaaagga cccggagac gccgtcggg agatccggg aagagtttt cttttctgc atgagcgtt cgagttcca tggaatcct ctagcaggg agatatggt ttggaacgc gaagagcac cgcagttgc ggcggtgtc cggatcttc ccctcggac cttgaaaat ccgggagag ggccacgtc gaggcgtcg cgccggttc gtacccata tccgcagca ggtctccaa ggtgaagag cctctagtc gatagaata atgattggc tctgaggat cggggcgtg tcgggcttg gcgaggaag cgggtcgcg gctgacgtg ccgggcctg tgcgaggtg aagcgttgt tccgtttcg gcggtcaat gtcgaatcc gagttcggt cccgtgcct tggcctccc gcggaaccg tcttgctgc gaggctccg ggtgcgccc gtcagggcg tccggaagt cctcttcgg ccgccattc aacggcttg gagagtgtg atcctgatc agatcacac ttgaaaagt tatgctgag tacgtccgc gtcgtgaga gtcggtaac tgtcccagg atggtctgg gataggcta aacctcagc aggggaagt tgtaggggc ctgccaccc ctacacttt attggtatg gcattcgat acccttaat gaagcctcg gacttggag gagcacggt tcccctcct cctcgcatt agacctgga accaactgt cttgacaac cccattgga cctatggga gcggaccat gctatggac atggattcc gaagacgaa gcgggggca cacggaccc cccgccgat aatgctcac ttaacgtca ggcgaaccc attgaaatc atcttgatg ttacccttt caaagcagg tcatgcggc atatgtctc aatgccgga aagggtaac ttccgggcg ttaagtcta gatgatgag gaacggcat ctgagggaa agacatcca ttgtcgcta atcctatac aaatgctct gattgcaag gggcagtat aggtcgaaa agagcggca ttatgccat gcgcccaaa tgtactggg cccacgcca gacccacag ggtaacgca ctaaggtgt catctgtgt ggtctggtc tgtaaaagt cagtcgggg gttactcag cacctccga cacagacac cctctcgtc agaaacacc caacgagcg gctgaggaa tctgccaga gctgagagg gcagctctc cctcgtccc ctgagaagg aacacacga cctgtgttt tcggaggag gacgaggct aaaatgctg gaattggag gatcccgcc ggcgcggaa gattccgtt ctgccggta gtggagacg gatgaaccg gccgaagag aacatcccc cttgagtat cccgagttg cctggggat gaggagggg gcccctgca tgttctcaa acaatcctc aacaccgag ggtcccgat ggtctcggg agcccgccg gtcccagtg gaggaggaa atggcatcc tcaggttcc acctccaac aatgtcgat acgggttgg agggagagt ataataact gccgctttg ggggttgaa attccaaag gccatctct caggaacca gcagctgtc atccaggag ctgcaagac gctcttcgc gaggcggtc atcggcgtt ttccctcag gaccgcctc gacgaaatg tacgaaaga gtgctcaag gtcgtaaat cctgacgat tcccaggaa cgaccaaag cgtcagagg aaaaagggt aagagtaga aatgctttc cggcggtat gtctactct caaacccag gacctattt aaaaagaat ccaggtcag ctggcccgc tatgtgagg gaagatgtg cgttggctg gaacagggc cgagtgcaa ctccagcgc gacaacatc gagcggatg tacaacaaa ctttggggc acaaagccg gacgtactc ccacctcac tgggattat cctctgcct ctggataca gcagatctg gagagtcac ccggggtat aaggttggt ggagaactg gctagtgtc tcctgcatg gccttcgct gacgacatt tttctaata gcggctaat gtacctcag acatgcacc ctgctacga gttacggag gactactta gagaggctg ggaatgagg atctcagcc ccgaaatgt acgtccttc gaaatacga ccgacaaaa gactcctgg tacgtagca gaccccggg ttgacactg acgaaggga gagaggatc ccggttgcg gccgtggat gccgtgttc agttatctc ggtgtggaa atatctcct tgggccggt attacatcg gagggcatt gagagagat tggcgtggt actctacat agagtccaa cgcctggcc ttaaaacca caccagaaa cttgaacta atctcccga tacctagtc ccccacttt ctctataaa ttggtggta acaatacca tcaatcacg ctgatcagg caactagac caggagctg agagtagtg gttaaacaa atatgccat ctaccgcag agcactgcg gacggtatg atttactgc cgcaaggta gatggcgga ctaggtatc cccaagttg gagatcgta actgtcacc tccatcttg aaggcgggt ctcaaattt agagactcg caagacaag attatgcaa gctctttgg ctagcttcg ggaatgtca tctcgatta agcacgcta gccaaagca acaagggtc caaccctgg ccacccaac aacatcaaa gacctcgat cgacataag gtggccagg aagagagaa gagcttgct aggtgggcg tctctgacc tcgcagggt aagtcggtt aagtccttt gccggcagc agaactgca aacgcttgg cttattaac aaaaagctc ctaaagcca agcacattt ataagtgcc ctccgcctg cgaggcaac gtagctggt gacagagtt gcgttgaac agagcaatc ccacaggcg aatctgatg tgtagaaga tgtgggtcc caacgtgag acactggga cacatcttg gggatttgt acgagtaca aacgccctt agaatcagt cgccatgat gagattaaa aatctcatc gtcgacgag gcagctaaa aaggacgac gaggtagca gtcacactg gaaccgacc atccggcat ccaatgcgc gggaatctc aaacccgac ctggtggta cagaaccgg gaaggggtc tacgtggtc gacgtcaca gtccgccac gaggacggg aatctcctt acacaggga cggcaagat aagctggat aaatatgag gtactgcta cctatcctc caggaacgg ctgggagcg ccgactgga gaagtccta ccaattgtg gtaggaacg aggggggcg atgcccaag gagacggtc gaagctctc aaaaagctc agaattacc gaccgccag acgctatta acgatatcg ctaatagcg ctgcgtatg tccgtgaaa atatatcat acattcatg gactacgca aacgcccgc cctcggcct ggtggggga gccaattac ccccacaga ctggagaag aaaatagga gatgatgag ttcccggat ataaagagg gaagataat aaggcgccc cggagctac gcaataatc gtgaaagga aaggatgag aatgagaat gcctctaaa attaaagag aagttattg aaggtagag acagatatc aaaatacag agggttaga ccgtctaga gttgggggg ggactggtg attgaaacc accagcgag aaggatctg cacaaggtg agacaaaaa ataatggag caaaaggaa gaatggagc atagaagat gcaaaaagg agaggccct tccattatg gtttatgac gtacctgtt catatagat aacgaggag tttgctaag gaagtatac aaaaagaac ttggcgacg gtactgaga gaggaagaa tttgtaaaa gattttcga atcgttagc aaaagaagt tcggagaaa gagcaaagt acaggcagt gggaaagaa aatgtctta atatgcgtg tcgccacaa atatgtatg aaagtgttt gtgtcgaat gaggtgcca cctgctgcg gcggtggtt atgaactgc aaaggagtc gatgtagta aaaatggag attgaggga gagagtagg ggagtgtgt gtaagcgta gcgggtgag tttggggat ttttgtgtt gcttctaca tactgtagg tatggttat gatttgcgg ccatatcta gaatggatt acgagagtg aaagaaaaa tgtgtgaat atgcctctt ctaattgga atggatgct aatgcggtc tctcccttg tggtacagt aagatgcat cgacgggaa cggggtgaa agggaggct cggggtgag cgacttgag gacctcgtg gcccaagaa gagtttctg attttaaat cagcctagt caaaaatac acgtttagt ggtcccatg ggagagtct gatattgat gtaacaatg tgcaataga gaatggatg tataagtat gaaacgagt tgggatgtg aatgaagaa tggggttgt agtgatcac aactgcata gtgatgtgt gtgaatggg agagaggga ggaggacgt gtgaggcga gcaaagaag aaaaggtgg agaatgaat ggggtggac tgggaaaaa tttaagaaa aaattgaac gaggttaga gacacagct ctttatagt ggggagtca attgatggg aaaatatac ctcctggag gcctggatc gaaactgct atgcggaaa gcaacgata aaaattaat gttggatct cgtgaggtt cgctggtgg actaacgag ctaggctct ctaaaagca gaagccagt aaggaaagg aaaagatgg cagagggct cggaaacga gctggttcg attggccgc agtgatcca tggagggag gtatataaa atttgcaag ggtggtatg agcgacaga gggttgtcg gtggtgaaa aggggtggt gtcttgacc aaatcgtgg aaggaaaca atggaagtt ttattggat gagtttttc ccagttgac agtgtggat gaaaataat ggggatgag gagagtgga gtaagtggc gatgaaaga ccaagggag attgaagaa tgggagctg gatgcaagc atccagaga atgaatggg aaaaaatct cctggaatg gatgagatt accccagaa atggtgaag gctgtatgg atgtgcatg aaagaggaa ctgattgag ctgtataat gaatgtgtg agaatggag tatttcccg gcgagatgg aaggaagct agagtggta atattgttg aaagggggt gacagggac aggactctt cccaggtca tacagggca atttgcctt ctgcctgtt cttgggaag gtccttgag agaataatg gttgaaaga ctgcatggt agaattgag aatataggt tcaaataga cagtatgga tttaagaaa ggaatggga acggaagat gcatggatg agagcaaaa aatgtagtg gtaaatagt gagaaaaag tatgttatg ggtttgttt gtggatttt aagggtgcg tttgacaac ttgaggtgg aatgtgctg ctggcgcgt ataagggag gtaggatgt ttggagtat ggtttgtgg agaagttac ttttgtaac aggaaggtg tgtgcggat ggtgaagga ggcgaaata tggaaacag gtgtggaag gggtgcccg caaggatcc atatgtggg ccctcggtg tggaacatg atgatggat gaattgctg aatgatctt gagagtctg aatgtggaa gtggttgcc tatgctgac gatgtctta tgtctgatt gagggtatg agtagagcc gaaattgaa gtaaaggga acagaagtg agtgatgta attgaaagg tggggtcga agggttggt gtggatgtg tctgaagac aaaacggtg atgatgttg atgaaaggg agtttggcg gacacgaga cctgcgaat gtgagaatg aaaggtaag gtagtacgg acagtgaag gaagttaag tatctagga atacatgtt ggagaacga atgaacttt agaccgcat gtggaacgt ctgaatgcg aaagtaatc gctgcgatg gggaggatg agaagggtc ctgaggaag gaatggggt gtaggcaag aaaacgacg agaaactgg tacaaggga ctgatggtt gcgtgtgtc gcatatggt gttggtgtg tggtgtgat gtgttaaag tatgaatat gcaagggtt ctggtaaac cgatgtcag agagtttgt ttgtatggg tgcctgcca gtatgtaag actgtgagt acggatgcg atgcaagtg ttgatgggt gaattgccg tgggatttg gagctcttg agaagaggt gtaaggtac aggattggt aggggtgcg cctttcggt gaggtaagc ccggtaagg gaggttgaa gtggttgag attggagtg aatgaaagc aaaaagttg ttggaagaa agactgtgg aaaacatgg caagaaaga tgggaagag agtgaaaat gggcggatt acgtatcga tttatcggg agagtggat tatgttaaa aagaacgag tggtttggt cccagtatg tatggcatg tttttaata acggggcat ggtagtatg aatgaattg ctgtttggt aggaatcta agtgtaact gataggtgt aaatgcggt gaggccgaa tcttggctt cacatcttg tatgattgt aggctgtat gaggacata agggaagaa taccatgct acaccttgt gttaattac agattggac atgacctcg atcaaagat gaagttctt caaccgcat gtcgatgac ctgatgagc cttttggat gaaagattt tcaacgatc gactccatc ccggaaatc gaaaatgcg acaaaggaa ctatacagc ggacttgcg gagattttt tcaaacacg ggaaaaaac gcaaaaatt ttgtgggaa ggcccgatt ggtggaacg aagaatttg agagatttc gaaaaattt cgagaatat tccgaccat cttttcgcg gaaatgacc agggccaag caaactttc aaagagaaa gtggagaag tggagacag aaatgctgg ttggaattc gccgagaat gacctggca aggaacctc tggggagtt gtctacaaa ttggcttct gaaaaactc aaaactcgt ggaattttg caatctttt caaaccgac gaggacgac aacataacc agggatttc caatcaacc atggaattt ctcattacg aatttactt ccggacgac gacactgac gccaacacc gaggagcaa cgtgtcact caaagcgat tttcgaaca accgctgcg gaagtgggg agtgacgct gtgatcacc gaggagaag gttctcacg ggacatgga aactttggt atccaccaa ttacgactt ggaaaaagc gaaaactca ttgtgtcca aattgcccc gactttgac gatgacccg gttcaccgc attttggaa tgccccctt ttcggggaa attcaggaa cggatccgg ggaatcacc ggaacctgg ccacctgac ctcactcag gtgccctac attgacaat gacgaagtg tttagtgca ctttccctg gacttgacc caaccggat gttaggacc ttacactgg tctagtcat gacatgatc cgctcattt tacggatca agtgcacct agtccagac aaggcgaag tgcgatttg tgcagtcaa aaaatgagc tacaaatca tcgacttcc aatatgaaa agacatgtg caacggaaa catcctacc gtaaaaatg aaacctgag gtggaaaag aataaaaat atatccgac aaagaagac atagtcaga gatcgcgaa gaaattcct tcgacttct tctttagat caggaaatt caagcgcat gaacaagct gaaaccccg aatatcaag caaccatcg aaaacaaga aagtcgaag caaaaccat gctacacct tgtgttaat tacagattg gacatgacc tcgatcaaa gatgaagtt cttcaaccg catgtcgat gacctcatg agccttttg gatgaaaga ttttcaacg atcgacttc atcccggaa atcgaaaat gcgacaaag gaactatac agcggactt gcggagatt tttctcaaa cactggaaa aaacgcaaa aattttgtg gaaaggtcc gattgggcc aagcaaact ttgaaagag aaagtggag aagcggaga ctgaaatgc tggttggaa gtcgccgag aatgacctg gcaagggaa ccctgggga gttgtctac aaattggcc tctgaaaag ttcaaaact cgtggaatt ttgcaatct tttcaaacc ggcgaggac aacaacata accagggat ttccaatca accatggaa tttctcatt atgaattta ctaccggac gacgacctg acgccaaca ccaaggagc aacgtgtca ctcaaagcg attttcgaa caaccactg cggaagtgg ggagtcctc acgggacat gggaacttt ggtattcac caattacga cttggaaaa agcgaaaac tcattgtgt ccaaattgc cccgactat gacgatgac ccggttcac cgcattttg gaatgcccc cttttcggg gaagttcag gaacggatc cggggtatc accggaacc tggccacct gaccttaca caggtcctc tacattgac aatgacgaa atgttcagt gcactttcc ctggacttg acccccccg cattactgt cgtgccgcc ttgcagagt gaacgacaa gcccagttg tttaaaaat ggagcaaga gtagaagct gtgaagaaa gagcaagtt acgaagaaa acggagaaa atcaagtgg aaactgaaa acagacgaa gcggaccaa ttccagata gaagaaaaa ttaaagaaa cgcattcct ttatcactc cagcccaaa ctagaagaa accttgaaa gaattggaa cgccaagga attattgag aaggtggaa tatccgaca gattgggtt aacagtcta gtgattgtt gagaagaaa acaggacag ctgagactc tgcttggac cctaaggat ttgaacaaa gccataaaa agggagcac tatatgata cccacagcc caggatata attccacgt ttagctggt aagaagatt tttacagta atagatatg agcgctgga ttctggcat gtgccttta gataaggaa agttcagat ttgtgttgt ttcaacacc catgacgag gacacgagt gcaatatta cagaaactg gacgaaatg acaactgac acgccaccc gaccaccag tggcgagag aatatcata tacagcccc atctcctct ggtcttgaa ctatccgcc caggagccg gcgaacagg ggctggata gataacaaa cctaaaggc tggaccgga cgggacttt gtgcgcgcc atccatctg cgcaccgct aacctacca tccagggga ataccctcc agccccctg aggaacggg aacggaagt gccggaacg ggtgtgact cacggtctc agaattctc cgccacaaa gccatagcc aaaaccatc gcgaagcac tgccagtct aacggttgg aaggttgaa ggggagctc cacgttcgc catccaagt gggcaactt ttcaagccc gatctagta gtgcatacc tcctcagaa aatatggtg acatacgcc gatgtccac ctcctagtg gttcccgcc ggtaacgga catctatcg ctgatggaa cttagctcg ctcttaaag aacggcgga cggggactt ggaatcgtg gtgtggttc agatgctcg gtcaatggt aatctccct acagttccc cccggcccc caggccggg gctcctgca ttcactgct gatcggaat gccaatggg aagccagcc caaaacccg tgcccatac tgtgcgcag tcgttcaca accgccaat ggccgcggg ttgcacata cggagggcg catccagac gaagctaat aacgccaag gacatcgaa cgcattctg caggcgccg caaataaac ctccccgaa atgcgtgat ggtgatgcg cctcgtcag actgatcct caacaggaa aaccctccc gagccccct tctttcgat ggggcgatc aggggcgct atcgccgac cttgtcgga ggggtcgac tggcagcgc ctcgggttt cagggagac cgtctctgt aacatcgcg agacgggct tgcgatggg agggatgtt tcgggccag ttgctgggc tggctgcgg gatgttttc cccgtcaag agggttagt actagaggg gatcagagc aacctagat gtcgacggc gcattggtg agcaggcat acggcgagg acaagggag tacgctcgg gcgcaggag ctgtaccgg aaggaacct aaggcgtgt ttggctcga atccttggt gatcgtcga gagggggca aatcggggt cctaatcgc aatctcgca ttcgtcgat ttttggcga ggcgtcttt tcggagggc tctgctgag gtcgaggga tgggcggag gaggtctcc gatcatggt gagcttgca cgccgggtg tgggacccc atctctatc gaggaggtg gtaaggtct cgggtccgg aatagtgca gcgccgggg cccgacgga atagcggtt tccgtgtgg aacaagctt ccgccggag accgctgcc cttcttttc aatgtgctg ctgttggga agatgtctg cctgcagaa ctcacccgc acgccaacg gtctgtatt ccaaagacc gacgcaccg cgaacacct gcagactat cggccgatt tcgatcgcg tcggccgga cttccacaa ccatttgtg gagtacgtc cggtccata tataggagt gcggagacc gtgttggag gacggcggg aggaggcac ttcgtgcaa gtgcggtgt ggggtgagg cagggagac ccactctct cctctcctc tttaacctg gtcttggac cgggctctg aagaggctg tccaccgag gtggacttt cgtctaacg gatgcgacc aaagttccg gcactcgct tttgcggac gacgtcgtt ttgtgcgcg accatccaa tctggatgt tctcgaagc ggagcttcg catagccgg tctcttgtt gaaccccaa caagtgtcg gccttatcc ttggtggct tctggacgt caccacaaa gtgaagctc gtgacaaaa cccaccttt aaagtagga caaaacacg attcaccaa gtggatgcg tccagtatt tggaagtac ctgggtatc cagtttagg ggtagtggg atgtgcgga tgcggttcg gagggtgtg gctgctggg ttgacgcgg atcacttgt gctccgctg aagcctcag cagaggatg cacctgctg cgagtcttt ttcttgccc aagttttat catgcttgg acatttggg agactcaat gcgggtgta ctgcgccgt caggacgtt gtggtccga acttcggtt cggacctgg ctacgcctc ccccacgat ataccggtg ggatacttt cacgctcct acgaagtcc ggggggctg aggatcccg cagttgtca cgattcatc ccgttcttg cgattaaag cggttcgac cggttaggg gtttccgcg gttgactat gtccgagag tgtgcattt acggatatt gcgaatcga aagatccgg tggtgccgt gggcgcctc ttgggcata gtggaccaa gtcgcgggt gggcggaac gcgcttgat gcatattgg acagcgcag cttcatcag tcggtggaa ggaagagca ctaagtgcg gttcaatgc gtccacggc ctctcggat gggtcgcgg acgcggcgc cgcaagtcc tcgaaaatc agcagtgat gttcctttg gttttacca tctgtaggg gcttcggcc ctcacggtt tcctcgggt ttcctattg tatttcgta aacccgaca aggagccct ttggccctc ctccttgaa catctctcc ttcgtccag gagtctgcc ttccaacaa aatgagaga ctggctaac ttctcacgg gaagacttc agcccccga tttaaaatc gggcctaaa gaccgaagc acaacgaca tgggttgtt gaaatcgac cgcaaaata cgaacagta atactacga gattggctg gcaattggg tggcagact tgcaaagta tcagaccat attagaata acaagatgc tataagtgt cagaaattt ggacatcaa agtaaagac tgccaaagc gaacagacc tgtggacgc tgtgctggt aattattca tttaaagag tgcaaagtt aaggaggaa aactcagta tgtgctaac tgtaaaagt tctaaactg ccagaagag gaacaaaaa caccaaacg gactctccg aaatgtccc acttacatc cgcaagatt aaagatctg atcggcaag actgactat ggctcctaa
- >TC031589 RA
cacacaccc attgggcga aagggaatc cggttccta ttccggaac ccggcagcg gaaccgtta ataattcgg gccctcgca agagagttc gtcggggca acccaaaag gacccggag acgccgtcg ggagatccg ggaagagtt ttcttttct gcatgagcg ttcgagttc catggaatc ctctagcag ggagatatg gtttggaac gcgaagagc accgcagtt gcggcggtg tccggatct tcccctcgg accttgaaa atccgggag agggccacg tcgaggcgt cgcgccggt tcgtaccca tatccgcag caggtctcc aaggtgaag agcctctag tcgatagaa taatgattg gctctgagg atcggggcg tgtcgggct tggcgagga agcgggtcg cggctgacg tgccgggcc tgtgcgagg tgaagcgtt gttccgttt cggcggtca atgtcgaat ccgagttcg gtcccgtgc cttggcctc ccgcggaac cgtcttgct gcgaggctc cgggtgcgc ccgtcaggg cgtccggaa gtcctcttc ggccgccat tcaacggct tggagagtg tgatcctga tcagatcac acttgaaaa gttatgctg agtacgtcc gcgtcgtga gagtcggta actgtccca ggatggtct gggataggc taaacctca gcaggggaa gttgtaggg gcctgccac ccctacact ttattggta tggcattcg ataccctta atgaagcct cggacttgg aggagcacg gttcccctc ctcctcgca ttagacctg gaaccaact gtcttgaca accccattg gacctatgg gagcggacc atgctatgg acatggatt ccgaagacg aagcggggg cacacggac cccccgccg ataatgctc acttaacgt caggcgaac ccattgaaa tcatcttga tgttaccct ttcaaagca ggtcatgcg gcatatgtc tcaatgccg gaaagggta acttccggg cgttaagtc tagatgatg aggaacggc atctgaggg aaagacatc cattgtcgc taatcctat acaaatgct ctgattgca aggggcagt ataggtcga aaagagcgg cattatgcc atgcgccca aatgtactg ggcccacgc cagacccac agggtaacg cactaaggt gtcatctgt gtggtctgg tctgtaaaa gtcagtcgg gggttactc agcacctcc gacacagac accctctcg tcagaaaca cccaacgag cggctgagg aatctgcca gagctgaga gggcagctc tccctcgtc ccctgagaa ggaacacac gacctgtgt tttcggagg aggacgagg ctaaaatgc tggaattgg aggatcccg ccggcgcgg aagattccg ttctgccgg tagtggaga cggatgaac cggccgaag agaacatcc cccttgagt atcccgagt tgcctgggg atgaggagg gggcccctg catgttctc aaacaatcc tcaacaccg agggtcccg atggtctcg ggagcccgc cggtcccag tggaggagg aaatggcat cctcaggtt ccacctcca acaatgtcg atacgggtt ggagggaga gtataataa ctgccgctt tgggggttg aaattccaa aggccatct ctcaggaac cagcagctg tcatccagg agctgcaag acgctcttc gcgaggcgg tcatcggcg ttttccctc aggaccgcc tcgacgaaa tgtacgaaa gagtgctca aggtcgtaa atcctgacg attcccagg aacgaccaa agcgtcaga ggaaaaagg gtaagagta gaaatgctt tccggcggt atgtctact ctcaaaccc aggacctat ttaaaaaga atccaggtc agctggccc gctatgtga gggaagatg tgcgttggc tggaacagg gccgagtgc aactccagc gcgacaaca tcgagcgga tgtacaaca aactttggg gcacaaagc cggacgtac tcccacctc actgggatt atcctctgc ctctggata cagcagatc tggagagtc acccggggt ataaggttg gtggagaac tggctagtg tctcctgca tggccttcg ctgacgaca tttttctaa tagcggcta atgtacctc agacatgca ccctgctac gagttacgg aggactact tagagaggc tgggaatga ggatctcag ccccgaaat gtacgtcct tcgaaatac gaccgacaa aagactcct ggtacgtag cagaccccg ggttgacac tgacgaagg gagagagga tcccggttg cggccgtgg atgccgtgt tcagttatc tcggtgtgg aaatatctc cttgggccg gtattacat cggagggca ttgagagag attggcgtg gtactctac atagagtcc aacgcctgg ccttaaaac cacaccaga aacttgaac taatctccc gatacctag tcccccact ttctctata aattggtgg taacaatac catcaatca cgctgatca ggcaactag accaggagc tgagagtag tggttaaac aaatatgcc atctaccgc agagcactg cggacggta tgatttact gccgcaagg tagatggcg gactaggta tccccaagt tggagatcg taactgtca cctccatct tgaaggcgg gtctcaaat ttagagact cgcaagaca agattatgc aagctcttt ggctagctt cgggaatgt catctcgat taagcacgc tagccaaag caacaaggg tccaaccct ggccaccca acaacatca aagacctcg atcgacata aggtggcca ggaagagag aagagcttg ctaggtggg cgtctctga cctcgcagg gtaagtcgg ttaagtcct ttgccggca gcagaactg caaacgctt ggcttatta acaaaaagc tcctaaagc caagcacat ttataagtg ccctccgcc tgcgaggca acgtagctg gtgacagag ttgcgttga acagagcaa tcccacagg cgaatctga tgtgtagaa gatgtgggt cccaacgtg agacactgg gacacatct tggggattt gtacgagta caaacgccc ttagaatca gtcgccatg atgagatta aaaatctca tcgtcgacg aggcagcta aaaaggacg acgaggtag cagtcacac tggaaccga ccatccggc atccaatgc gcgggaatc tcaaacccg acctggtgg tacagaacc gggaagggg tctacgtgg tcgacgtca cagtccgcc acgaggacg ggaatctcc ttacacagg gacggcaag ataagctgg ataaatatg aggtactgc tacctatcc tccaggaac ggctgggag cgccgactg gagaagtcc taccaattg tggtaggaa cgagggggg cgatgccca aggagacgg tcgaagctc tcaaaaagc tcagaatta ccgaccgcc agacgctat taacgatat cgctaatag cgctgcgta tgtccgtga aaatatatc atacattca tggactacg caaacgccc gccctcggc ctggtgggg gagccaatt acccccaca gactggaga agaaaatag gagatgatg agttcccgg atataaaga gggaagata ataaggcgc cccggagct acgcaataa tcgtgaaag gaaaggatg agaatgaga atgcctcta aaattaaag agaagttat tgaaggtag agacagata tcaaaatac agagggtta gaccgtcta gagttgggg ggggactgg tgattgaaa ccaccagcg agaaggatc tgcacaagg tgagacaaa aaataatgg agcaaaagg aagaatgga gcatagaag atgcaaaaa ggagaggcc cttccatta tggtttatg acgtacctg ttcatatag ataacgagg agtttgcta aggaagtat acaaaaaga acttggcga cggtactga gagaggaag aatttgtaa aagattttc gaatcgtta gcaaaagaa gttcggaga aagagcaaa gtacaggca gtgggaaag aaaatgtct taatatgcg tgtcgccac aaatatgta tgaaagtgt ttgtgtcga atgaggtgc cacctgctg cggcggtgg ttatgaact gcaaaggag tcgatgtag taaaaatgg agattgagg gagagagta ggggagtgt gtgtaagcg tagcgggtg agtttgggg atttttgtg ttgcttcta catactgta ggtatggtt atgatttgc ggccatatc tagaatgga ttacgagag tgaaagaaa aatgtgtga atatgcctc ttctaattg gaatggatg ctaatgcgg tctctccct tgtggtaca gtaagatgc atcgacggg aacggggtg aaagggagg ctcggggtg agcgacttg aggacctcg tggcccaag aagagtttc tgattttaa atcagccta gtcaaaaat acacgttta gtggtccca tgggagagt ctgatattg atgtaacaa tgtgcaata gagaatgga tgtataagt atgaaacga gttgggatg tgaatgaag aatggggtt gtagtgatc acaactgca tagtgatgt gtgtgaatg ggagagagg gaggaggac gtgtgaggc gagcaaaga agaaaaggt ggagaatga atggggtgg actgggaaa aatttaaga aaaaattga acgaggtta gagacacag ctctttata gtggggagt caattgatg ggaaaatat acctcctgg aggcctgga tcgaaactg ctatgcgga aagcaacga taaaaatta atgttggat ctcgtgagg ttcgctggt ggactaacg agctaggct ctctaaaag cagaagcca gtaaggaaa ggaaaagat ggcagaggg ctcggaaac gagctggtt cgattggcc gcagtgatc catggaggg aggtatata aaatttgca agggtggta tgagcgaca gagggttgt cggtggtga aaaggggtg gtgtcttga ccaaatcgt ggaaggaaa caatggaag ttttattgg atgagtttt tcccagttg acagtgtgg atgaaaata atggggatg aggagagtg gagtaagtg gcgatgaaa gaccaaggg agattgaag aatgggagc tggatgcaa gcatccaga gaatgaatg ggaaaaaat ctcctggaa tggatgaga ttaccccag aaatggtga aggctgtat ggatgtgca tgaaagagg aactgattg agctgtata atgaatgtg tgagaatgg agtatttcc cggcgagat ggaaggaag ctagagtgg taatattgt tgaaagggg gtgacaggg acaggactc ttcccaggt catacaggg caatttgcc ttctgcctg ttcttggga aggtccttg agagaataa tggttgaaa gactgcatg gtagaattg agaatatag gttcaaata gacagtatg gatttaaga aaggaatgg gaacggaag atgcatgga tgagagcaa aaaatgtag tggtaaata gtgagaaaa agtatgtta tgggtttgt ttgtggatt ttaagggtg cgtttgaca acttgaggt ggaatgtgc tgctggcgc gtataaggg aggtaggat gtttggagt atggtttgt ggagaagtt acttttgta acaggaagg tgtgtgcgg atggtgaag gaggcgaaa tatggaaac aggtgtgga aggggtgcc cgcaaggat ccatatgtg ggccctcgg tgtggaaca tgatgatgg atgaattgc tgaatgatc ttgagagtc tgaatgtgg aagtggttg cctatgctg acgatgtct tatgtctga ttgagggta tgagtagag ccgaaattg aagtaaagg gaacagaag tgagtgatg taattgaaa ggtggggtc gaagggttg gtgtggatg tgtctgaag acaaaacgg tgatgatgt tgatgaaag ggagtttgg cggacacga gacctgcga atgtgagaa tgaaaggta aggtagtac ggacagtga aggaagtta agtatctag gaatacatg ttggagaac gaatgaact ttagaccgc atgtggaac gtctgaatg cgaaagtaa tcgctgcga tggggagga tgagaaggg tcctgagga aggaatggg gtgtaggca agaaaacga cgagaaact ggtacaagg gactgatgg ttgcgtgtg tcgcatatg gtgttggtg tgtggtgtg atgtgttaa agtatgaat atgcaaggg ttctggtaa accgatgtc agagagttt gtttgtatg ggtgcctgc cagtatgta agactgtga gtacggatg cgatgcaag tgttgatgg gtgaattgc cgtgggatt tggagctct tgagaagag gtgtaaggt acaggattg gtaggggtg cgcctttcg gtgaggtaa gcccggtaa gggaggttg aagtggttg agattggag tgaatgaaa gcaaaaagt tgttggaag aaagactgt ggaaaacat ggcaagaaa gatgggaag agagtgaaa atgggcgga ttacgtatc gatttatcg ggagagtgg attatgtta aaaagaacg agtggtttg gtcccagta tgtatggca tgtttttaa taacggggc atggtagta tgaatgaat tgctgtttg gtaggaatc taagtgtaa ctgataggt gtaaatgcg gtgaggccg aatcttggc ttcacatct tgtatgatt gtaggctgt atgaggaca taagggaag aataccatg ctacacctt gtgttaatt acagattgg acatgacct cgatcaaag atgaagttc ttcaaccgc atgtcgatg acctgatga gccttttgg atgaaagat tttcaacga tcgactcca tcccggaaa tcgaaaatg cgacaaagg aactataca gcggacttg cggagattt tttcaaaca cgggaaaaa acgcaaaaa ttttgtggg aaggcccga ttggtggaa cgaagaatt tgagagatt tcgaaaaat ttcgagaat attccgacc atcttttcg cggaaatga ccagggcca agcaaactt tcaaagaga aagtggaga agtggagac agaaatgct ggttggaat tcgccgaga atgacctgg caaggaacc tctggggag ttgtctaca aattggctt ctgaaaaac tcaaaactc gtggaattt tgcaatctt ttcaaaccg acgaggacg acaacataa ccagggatt tccaatcaa ccatggaat ttctcatta cgaatttac ttccggacg acgacactg acgccaaca ccgaggagc aacgtgtca ctcaaagcg attttcgaa caaccgctg cggaagtgg ggagtgacg ctgtgatca ccgaggaga aggttctca cgggacatg gaaactttg gtatccacc aattacgac ttggaaaaa gcgaaaact cattgtgtc caaattgcc ccgactttg acgatgacc cggttcacc gcattttgg aatgccccc ttttcgggg aaattcagg aacggatcc ggggaatca ccggaacct ggccacctg acctcactc aggtgccct acattgaca atgacgaag tgtttagtg cactttccc tggacttga cccaaccgg atgttagga ccttacact ggtctagtc atgacatga tccgctcat tttacggat caagtgcac ctagtccag acaaggcga agtgcgatt tgtgcagtc aaaaaatga gctacaaat catcgactt ccaatatga aaagacatg tgcaacgga aacatccta ccgtaaaaa tgaaacctg aggtggaaa agaataaaa atatatccg acaaagaag acatagtca gagatcgcg aagaaattc cttcgactt cttctttag atcaggaaa ttcaagcgc atgaacaag ctgaaaccc cgaatatca agcaaccat cgaaaacaa gaaagtcga agcaaaacc atgctacac cttgtgtta attacagat tggacatga cctcgatca aagatgaag ttcttcaac cgcatgtcg atgacctca tgagccttt tggatgaaa gattttcaa cgatcgact tcatcccgg aaatcgaaa atgcgacaa aggaactat acagcggac ttgcggaga tttttctca aacactgga aaaaacgca aaaattttg tggaaaggt ccgattggg ccaagcaaa ctttgaaag agaaagtgg agaagcgga gactgaaat gctggttgg aagtcgccg agaatgacc tggcaaggg aaccctggg gagttgtct acaaattgg cctctgaaa agttcaaaa ctcgtggaa ttttgcaat cttttcaaa ccggcgagg acaacaaca taaccaggg atttccaat caaccatgg aatttctca ttatgaatt tactaccgg acgacgacc tgacgccaa caccaagga gcaacgtgt cactcaaag cgattttcg aacaaccac tgcggaagt ggggagtcc tcacgggac atgggaact ttggtattc accaattac gacttggaa aaagcgaaa actcattgt gtccaaatt gccccgact atgacgatg acccggttc accgcattt tggaatgcc cccttttcg gggaagttc aggaacgga tccggggta tcaccggaa cctggccac ctgacctta cacaggtcc tctacattg acaatgacg aaatgttca gtgcacttt ccctggact tgacccccc cgcattact gtcgtgccg ccttgcaga gtgaacgac aagcccagt tgtttaaaa atggagcaa gagtagaag ctgtgaaga aagagcaag ttacgaaga aaacggaga aaatcaagt ggaaactga aaacagacg aagcggacc aattccaga tagaagaaa aattaaaga aacgcattc ctttatcac tccagccca aactagaag aaaccttga aagaattgg aacgccaag gaattattg agaaggtgg aatatccga cagattggg ttaacagtc tagtgattg ttgagaaga aaacaggac agctgagac tctgcttgg accctaagg atttgaaca aagccataa aaagggagc actatatga tacccacag cccaggata taattccac gtttagctg gtaagaaga tttttacag taatagata tgagcgctg gattctggc atgtgcctt tagataagg aaagttcag atttgtgtt gtttcaaca cccatgacg aggacacga gtgcaatat tacagaaac tggacgaaa tgacaactg acacgccac ccgaccacc agtggcgag agaatatca tatacagcc ccatctcct ctggtcttg aactatccg cccaggagc cggcgaaca ggggctgga tagataaca aacctaaag gctggaccg gacgggact ttgtgcgcg ccatccatc tgcgcaccg ctaacctac catccaggg gaataccct ccagccccc tgaggaacg ggaacggaa gtgccggaa cgggtgtga ctcacggtc tcagaattc tccgccaca aagccatag ccaaaacca tcgcgaagc actgccagt ctaacggtt ggaaggttg aaggggagc tccacgttc gccatccaa gtgggcaac ttttcaagc ccgatctag tagtgcata cctcctcag aaaatatgg tgacatacg ccgatgtcc acctcctag tggttcccg ccggtaacg gacatctat cgctgatgg aacttagct cgctcttaa agaacggcg gacggggac ttggaatcg tggtgtggt tcagatgct cggtcaatg gtaatctcc ctacagttc cccccggcc cccaggccg gggctcctg cattcactg ctgatcgga atgccaatg ggaagccag cccaaaacc cgtgcccat actgtgcgc agtcgttca caaccgcca atggccgcg ggttgcaca tacggaggg cgcatccag acgaagcta ataacgcca aggacatcg aacgcattc tgcaggcgc cgcaaataa acctccccg aaatgcgtg atggtgatg cgcctcgtc agactgatc ctcaacagg aaaaccctc ccgagcccc cttctttcg atggggcga tcaggggcg ctatcgccg accttgtcg gaggggtcg actggcagc gcctcgggt ttcagggag accgtctct gtaacatcg cgagacggg cttgcgatg ggagggatg tttcgggcc agttgctgg gctggctgc gggatgttt tccccgtca agagggtta gtactagag gggatcaga gcaacctag atgtcgacg gcgcattgg tgagcaggc atacggcga ggacaaggg agtacgctc gggcgcagg agctgtacc ggaaggaac ctaaggcgt gtttggctc gaatccttg gtgatcgtc gagaggggg caaatcggg gtcctaatc gcaatctcg cattcgtcg atttttggc gaggcgtct tttcggagg gctctgctg aggtcgagg gatgggcgg aggaggtct ccgatcatg gtgagcttg cacgccggg tgtgggacc ccatctcta tcgaggagg tggtaaggt ctcgggtcc ggaatagtg cagcgccgg ggcccgacg gaatagcgg tttccgtgt ggaacaagc ttccgccgg agaccgctg cccttcttt tcaatgtgc tgctgttgg gaagatgtc tgcctgcag aactcaccc gcacgccaa cggtctgta ttccaaaga ccgacgcac cgcgaacac ctgcagact atcggccga tttcgatcg cgtcggccg gacttccac aaccatttg tggagtacg tccggtcca tatatagga gtgcggaga ccgtgttgg aggacggcg ggaggaggc acttcgtgc aagtgcggt gtggggtga ggcagggag acccactct ctcctctcc tctttaacc tggtcttgg accgggctc tgaagaggc tgtccaccg aggtggact ttcgtctaa cggatgcga ccaaagttc cggcactcg cttttgcgg acgacgtcg ttttgtgcg cgaccatcc aatctggat gttctcgaa gcggagctt cgcatagcc ggtctcttg ttgaacccc aacaagtgt cggccttat ccttggtgg cttctggac gtcaccaca aagtgaagc tcgtgacaa aacccacct ttaaagtag gacaaaaca cgattcacc aagtggatg cgtccagta tttggaagt acctgggta tccagttta ggggtagtg ggatgtgcg gatgcggtt cggagggtg tggctgctg ggttgacgc ggatcactt gtgctccgc tgaagcctc agcagagga tgcacctgc tgcgagtct ttttcttgc ccaagtttt atcatgctt ggacatttg ggagactca atgcgggtg tactgcgcc gtcaggacg ttgtggtcc gaacttcgg ttcggacct ggctacgcc tcccccacg atataccgg tgggatact ttcacgctc ctacgaagt ccggggggc tgaggatcc cgcagttgt cacgattca tcccgttct tgcgattaa agcggttcg accggttag gggtttccg cggttgact atgtccgag agtgtgcat ttacggata ttgcgaatc gaaagatcc ggtggtgcc gtgggcgcc tcttgggca tagtggacc aagtcgcgg gtgggcgga acgcgcttg atgcatatt ggacagcgc agcttcatc agtcggtgg aaggaagag cactaagtg cggttcaat gcgtccacg gcctctcgg atgggtcgc ggacgcggc gccgcaagt cctcgaaaa tcagcagtg atgttcctt tggttttac catctgtag gggcttcgg ccctcacgg tttcctcgg gtttcctat tgtatttcg taaacccga caaggagcc ctttggccc tcctccttg aacatctct ccttcgtcc aggagtctg ccttccaac aaaatgaga gactggcta acttctcac gggaagact tcagccccc gatttaaaa tcgggccta aagaccgaa gcacaacga catgggttg ttgaaatcg accgcaaaa tacgaacag taatactac gagattggc tggcaattg ggtggcaga cttgcaaag tatcagacc atattagaa taacaagat gctataagt gtcagaaat ttggacatc aaagtaaag actgccaaa gcgaacaga cctgtggac gctgtgctg gtaattatt catttaaag agtgcaaag ttaaggagg aaaactcag tatgtgcta actgtaaaa gttctaaac tgccagaag aggaacaaa aacaccaaa cggactctc cgaaatgtc ccacttaca tccgcaaga ttaaagatc tgatcggca agactgact atggctcct aacgcctca ctcaatgtt aaat
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