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Gene information
General information
TC012502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9529315..9531126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012502
    iBeetle screen iB_07637

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012502 RA
      MLVLTTVLT AAAVFLLFY LFYCYKTIK QHIYSLISL 
      SYLPGPPVN DIISGNILP LYTSAENIF KQLREWGRL 
      YYPIYKLNA AHLAAANIL SPEDCELVL SNPTHMEKS 
      AIYNLLHDW LGTGLLTST GLKWQTRRK ILTPAFHFS 
      ILQQFVAIF NEETDKLVE VLKEECYKP FINVNAHVA 
      QFTLKTIAE TAMGTKLRF TTRKETIYK QSIVDMGEF 
      LLYRLLRCW LISKCIYVF NPRYYLEKK VTRRLHRFT 
      KSVIAERQE NFKEIVVPE TDEVYTGKR RLAMLDLLL 
      TAKNKEGLI DDEGIREEV DTFMFEGHD TTAAALGFA 
      LMVLAGHKE VQDKIVEEM NEVLGDIKK KPTYQDLQE 
      MKYLERCVK EVLRLYPSV HFISRKLGE DLVTHSGHK 
      LAKGSIVNL HIYDLHHNP AIYPDPEKF DPDRFLPEN 
      CQKRHPFAY LPFSAGPRN CIGKKFAML ELKAAICGI 
      LANFTLEPI DIPETIVLV VDIVLRTKE GIKVRFIPR 
      V
    CDS sequence
    • >TC012502 RA
      atgctcgtt ttgacaaca gtcttaaca gcagctgct 
      gtttttctc ctcttctac ctgttttat tgctacaaa 
      acaataaaa cagcacatc tacagcctc atctcgctc 
      agctaccta ccaggaccc ccagtgaac gacatcatt 
      tcaggaaat atcctccca ctgtacact agcgcagaa 
      aacatcttc aaacagttg cgggaatgg ggccgactc 
      tactacccc atctacaag ctaaatgcc gcccatttg 
      gccgccgcc aacattctc agtccggaa gactgcgag 
      ctggtttta tccaaccca actcatatg gaaaagtcg 
      gcaatctat aatctcctc catgactgg ttagggacc 
      gggcttttg acaagcact ggacttaaa tggcaaaca 
      cgtcgaaaa atcttaacc ccagctttc catttcagt 
      attttgcaa cagtttgtt gctattttt aacgaagaa 
      accgataag cttgtcgaa gttttgaaa gaggagtgt 
      tacaaacct ttcatcaat gtcaatgcg catgtagcc 
      caatttacc ttgaaaact atcgcagag acagcaatg 
      gggaccaaa ctcaggttt accacacga aaagagact 
      atttacaag cagtcgatc gtcgacatg ggggagttc 
      ctcttgtat cgtcttttg agatgttgg ctaatatct 
      aaatgtatt tacgtcttc aatccgcgt tattatctt 
      gagaaaaaa gttacaaga cgtttgcac cgatttacg 
      aaaagtgta atcgccgaa cgacaggag aatttcaaa 
      gaaattgta gtaccggaa acagatgag gtctacaca 
      ggaaaaagg cgactcgcc atgttggat cttctgtta 
      acagcaaag aacaaagag ggcctgatt gatgatgaa 
      ggaattcgt gaagaggtc gatactttc atgtttgaa 
      ggccacgac accactgca gcagctcta ggttttgcc 
      ctaatggtc ctagcaggg cacaaggag gtccaggac 
      aaaattgtg gaagaaatg aacgaagtc ttgggcgac 
      attaagaaa aaacccact tatcaggat ctacaagag 
      atgaaatac ctggaacga tgtgtgaag gaagtactg 
      aggttgtac cccagtgtt cacttcatt tcgaggaaa 
      ctgggcgag gatttggtc actcacagt ggtcacaag 
      ttggccaaa ggctccatt gtcaatttg cacatctac 
      gatttgcac cacaatcct gccatttat cctgatcct 
      gagaagttt gatcctgat cggtttttg cctgagaac 
      tgccagaaa aggcatcct ttcgcctac ttgcccttt 
      agtgccggt cccagaaac tgtatcggg aaaaaattc 
      gccatgttg gaactcaaa gcggccatt tgtggcatt 
      ttggcgaac ttcactctc gaaccgatc gatatccct 
      gagactatt gtactagtc gttgatata gtactgaga 
      accaaagaa gggatcaag gttaggttt attccccga 
      gtatga
    mRNA sequence
    • >TC012502 RA
      gtcaaacaa aaatgctcg ttttgacaa cagtcttaa 
      cagcagctg ctgtttttc tcctcttct acctgtttt 
      attgctaca aaacaataa aacagcaca tctacagcc 
      tcatctcgc tcagctacc taccaggac ccccagtga 
      acgacatca tttcaggaa atatcctcc cactgtaca 
      ctagcgcag aaaacatct tcaaacagt tgcgggaat 
      ggggccgac tctactacc ccatctaca agctaaatg 
      ccgcccatt tggccgccg ccaacattc tcagtccgg 
      aagactgcg agctggttt tatccaacc caactcata 
      tggaaaagt cggcaatct ataatctcc tccatgact 
      ggttaggga ccgggcttt tgacaagca ctggactta 
      aatggcaaa cacgtcgaa aaatcttaa ccccagctt 
      tccatttca gtattttgc aacagtttg ttgctattt 
      ttaacgaag aaaccgata agcttgtcg aagttttga 
      aagaggagt gttacaaac ctttcatca atgtcaatg 
      cgcatgtag cccaattta ccttgaaaa ctatcgcag 
      agacagcaa tggggacca aactcaggt ttaccacac 
      gaaaagaga ctatttaca agcagtcga tcgtcgaca 
      tgggggagt tcctcttgt atcgtcttt tgagatgtt 
      ggctaatat ctaaatgta tttacgtct tcaatccgc 
      gttattatc ttgagaaaa aagttacaa gacgtttgc 
      accgattta cgaaaagtg taatcgccg aacgacagg 
      agaatttca aagaaattg tagtaccgg aaacagatg 
      aggtctaca caggaaaaa ggcgactcg ccatgttgg 
      atcttctgt taacagcaa agaacaaag agggcctga 
      ttgatgatg aaggaattc gtgaagagg tcgatactt 
      tcatgtttg aaggccacg acaccactg cagcagctc 
      taggttttg ccctaatgg tcctagcag ggcacaagg 
      aggtccagg acaaaattg tggaagaaa tgaacgaag 
      tcttgggcg acattaaga aaaaaccca cttatcagg 
      atctacaag agatgaaat acctggaac gatgtgtga 
      aggaagtac tgaggttgt accccagtg ttcacttca 
      tttcgagga aactgggcg aggatttgg tcactcaca 
      gtggtcaca agttggcca aaggctcca ttgtcaatt 
      tgcacatct acgatttgc accacaatc ctgccattt 
      atcctgatc ctgagaagt ttgatcctg atcggtttt 
      tgcctgaga actgccaga aaaggcatc ctttcgcct 
      acttgccct ttagtgccg gtcccagaa actgtatcg 
      ggaaaaaat tcgccatgt tggaactca aagcggcca 
      tttgtggca ttttggcga acttcactc tcgaaccga 
      tcgatatcc ctgagacta ttgtactag tcgttgata 
      tagtactga gaaccaaag aagggatca aggttaggt 
      ttattcccc gagtatgag cgttgttac aataaagtt 
      ttttaagca aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY