GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07637 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012502 RA
MLVLTTVLT AAAVFLLFY LFYCYKTIK QHIYSLISL SYLPGPPVN DIISGNILP LYTSAENIF KQLREWGRL YYPIYKLNA AHLAAANIL SPEDCELVL SNPTHMEKS AIYNLLHDW LGTGLLTST GLKWQTRRK ILTPAFHFS ILQQFVAIF NEETDKLVE VLKEECYKP FINVNAHVA QFTLKTIAE TAMGTKLRF TTRKETIYK QSIVDMGEF LLYRLLRCW LISKCIYVF NPRYYLEKK VTRRLHRFT KSVIAERQE NFKEIVVPE TDEVYTGKR RLAMLDLLL TAKNKEGLI DDEGIREEV DTFMFEGHD TTAAALGFA LMVLAGHKE VQDKIVEEM NEVLGDIKK KPTYQDLQE MKYLERCVK EVLRLYPSV HFISRKLGE DLVTHSGHK LAKGSIVNL HIYDLHHNP AIYPDPEKF DPDRFLPEN CQKRHPFAY LPFSAGPRN CIGKKFAML ELKAAICGI LANFTLEPI DIPETIVLV VDIVLRTKE GIKVRFIPR V
- >TC012502 RA
atgctcgtt ttgacaaca gtcttaaca gcagctgct gtttttctc ctcttctac ctgttttat tgctacaaa acaataaaa cagcacatc tacagcctc atctcgctc agctaccta ccaggaccc ccagtgaac gacatcatt tcaggaaat atcctccca ctgtacact agcgcagaa aacatcttc aaacagttg cgggaatgg ggccgactc tactacccc atctacaag ctaaatgcc gcccatttg gccgccgcc aacattctc agtccggaa gactgcgag ctggtttta tccaaccca actcatatg gaaaagtcg gcaatctat aatctcctc catgactgg ttagggacc gggcttttg acaagcact ggacttaaa tggcaaaca cgtcgaaaa atcttaacc ccagctttc catttcagt attttgcaa cagtttgtt gctattttt aacgaagaa accgataag cttgtcgaa gttttgaaa gaggagtgt tacaaacct ttcatcaat gtcaatgcg catgtagcc caatttacc ttgaaaact atcgcagag acagcaatg gggaccaaa ctcaggttt accacacga aaagagact atttacaag cagtcgatc gtcgacatg ggggagttc ctcttgtat cgtcttttg agatgttgg ctaatatct aaatgtatt tacgtcttc aatccgcgt tattatctt gagaaaaaa gttacaaga cgtttgcac cgatttacg aaaagtgta atcgccgaa cgacaggag aatttcaaa gaaattgta gtaccggaa acagatgag gtctacaca ggaaaaagg cgactcgcc atgttggat cttctgtta acagcaaag aacaaagag ggcctgatt gatgatgaa ggaattcgt gaagaggtc gatactttc atgtttgaa ggccacgac accactgca gcagctcta ggttttgcc ctaatggtc ctagcaggg cacaaggag gtccaggac aaaattgtg gaagaaatg aacgaagtc ttgggcgac attaagaaa aaacccact tatcaggat ctacaagag atgaaatac ctggaacga tgtgtgaag gaagtactg aggttgtac cccagtgtt cacttcatt tcgaggaaa ctgggcgag gatttggtc actcacagt ggtcacaag ttggccaaa ggctccatt gtcaatttg cacatctac gatttgcac cacaatcct gccatttat cctgatcct gagaagttt gatcctgat cggtttttg cctgagaac tgccagaaa aggcatcct ttcgcctac ttgcccttt agtgccggt cccagaaac tgtatcggg aaaaaattc gccatgttg gaactcaaa gcggccatt tgtggcatt ttggcgaac ttcactctc gaaccgatc gatatccct gagactatt gtactagtc gttgatata gtactgaga accaaagaa gggatcaag gttaggttt attccccga gtatga
- >TC012502 RA
gtcaaacaa aaatgctcg ttttgacaa cagtcttaa cagcagctg ctgtttttc tcctcttct acctgtttt attgctaca aaacaataa aacagcaca tctacagcc tcatctcgc tcagctacc taccaggac ccccagtga acgacatca tttcaggaa atatcctcc cactgtaca ctagcgcag aaaacatct tcaaacagt tgcgggaat ggggccgac tctactacc ccatctaca agctaaatg ccgcccatt tggccgccg ccaacattc tcagtccgg aagactgcg agctggttt tatccaacc caactcata tggaaaagt cggcaatct ataatctcc tccatgact ggttaggga ccgggcttt tgacaagca ctggactta aatggcaaa cacgtcgaa aaatcttaa ccccagctt tccatttca gtattttgc aacagtttg ttgctattt ttaacgaag aaaccgata agcttgtcg aagttttga aagaggagt gttacaaac ctttcatca atgtcaatg cgcatgtag cccaattta ccttgaaaa ctatcgcag agacagcaa tggggacca aactcaggt ttaccacac gaaaagaga ctatttaca agcagtcga tcgtcgaca tgggggagt tcctcttgt atcgtcttt tgagatgtt ggctaatat ctaaatgta tttacgtct tcaatccgc gttattatc ttgagaaaa aagttacaa gacgtttgc accgattta cgaaaagtg taatcgccg aacgacagg agaatttca aagaaattg tagtaccgg aaacagatg aggtctaca caggaaaaa ggcgactcg ccatgttgg atcttctgt taacagcaa agaacaaag agggcctga ttgatgatg aaggaattc gtgaagagg tcgatactt tcatgtttg aaggccacg acaccactg cagcagctc taggttttg ccctaatgg tcctagcag ggcacaagg aggtccagg acaaaattg tggaagaaa tgaacgaag tcttgggcg acattaaga aaaaaccca cttatcagg atctacaag agatgaaat acctggaac gatgtgtga aggaagtac tgaggttgt accccagtg ttcacttca tttcgagga aactgggcg aggatttgg tcactcaca gtggtcaca agttggcca aaggctcca ttgtcaatt tgcacatct acgatttgc accacaatc ctgccattt atcctgatc ctgagaagt ttgatcctg atcggtttt tgcctgaga actgccaga aaaggcatc ctttcgcct acttgccct ttagtgccg gtcccagaa actgtatcg ggaaaaaat tcgccatgt tggaactca aagcggcca tttgtggca ttttggcga acttcactc tcgaaccga tcgatatcc ctgagacta ttgtactag tcgttgata tagtactga gaaccaaag aagggatca aggttaggt ttattcccc gagtatgag cgttgttac aataaagtt ttttaagca aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
