Status
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Gene information
General information
TC012437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7384714..7389988
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012437
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07629

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012437 RA
      MDEAEASLG TQQIPEASE SLFSTIDII LLTLLIGGV 
      AWWFLNKQK KKDVAATAR SYSIQPTSM TLQTSSDNS 
      FIKKLKSSG RSLVVFYGS QTGTGEEFA GRLAKEGVR 
      YRLKGMVAD PEECDMEEL VNLKSIPNS LAVFCLATY 
      GEGDPTDNA MEFYEWLQN GDADLSGLN YAVFGLGNK 
      TYEHYNQVA IYVDKRLEE LGATRVFDL GLGDDDANI 
      EDDFITWKD KFWPAVCEH FGIESTGED VSMRQYRLQ 
      EFKDEVPEK LYTGEMARL HSLKNQRPP FDAKNPFLA 
      KILVNRELH KGNDRSCMH IEFDIEGSK MRYDAGDHV 
      AVYPINDSD LVEKIGKLT NTDLDTIFT LINTDEESS 
      KKHPFPCPT TYRTALTHY LDITMNPRT HVLKELSEY 
      CTDPAEKEK LKTMASTSP EGKALYQQW VNEDNRNIV 
      HILEDMPSC KPKLDHLCE LLPRLQPRY YSISSSPKL 
      YPNTVHITA VVVEYKTPT GRHNKGVCT TWLALKKPA 
      PGQEPPTAP IFIRKSQFR LPTKTQTPI IMIGPGTGL 
      APFRGFIQE RHQAKEEDK AVGETVLYF GCRKKAEDF 
      IYEEELLNY EKSGLLTLH LAFSRDQAH KVYVSHLLE 
      KNREEVWRI IGENNGHLY ICGDAKSMA PEVRNIVMK 
      IIQDKGQMT EQQASAYLK KMETQKRYS ADVWS
    CDS sequence
    • >TC012437 RA
      atggacgaa gcagaagcg agtctcggc acccagcag 
      atccccgaa gcctccgag tccctcttc agcacaatt 
      gacatcatc ctgctgacg ctcttaatc ggcggagtc 
      gcctggtgg ttcctaaac aagcagaag aagaaagat 
      gtggccgcc accgcacgc tcatactcc atccaaccc 
      acgtccatg accctccaa accagctcc gacaactcc 
      ttcatcaaa aaactcaag tcgtcggga cgcagcctg 
      gtggtgttc tacgggagc cagacggga acgggggaa 
      gagttcgcc gggaggctg gccaaggag ggcgtcagg 
      taccggctg aaggggatg gtggcggac cccgaggag 
      tgcgacatg gaggagctg gtgaacttg aaatcgatc 
      cccaacagt ctggctgtg ttttgtttg gcgacgtac 
      ggggaaggt gaccccact gacaatgcc atggagttc 
      tacgagtgg ctgcaaaac ggcgacgct gatttatca 
      ggcctcaat tatgcggtg tttggcttg gggaacaaa 
      acctacgag cactacaac caagtcgcc atctatgtt 
      gacaagaga ctagaggag ctgggggcc acgagagtg 
      tttgatctc ggactgggc gacgacgac gccaacatt 
      gaagatgac tttatcacg tggaaggac aagttctgg 
      cccgcggtg tgcgaacac ttcggcatc gagtcaacc 
      ggcgaggac gtcagcatg cgccaatac cgcctccag 
      gagttcaaa gacgaagtc ccggagaag ctctacact 
      ggtgagatg gcgcgcctc cactccctc aaaaaccag 
      cggccgccc ttcgacgcc aaaaacccc ttcctggcg 
      aaaattctg gtgaatcgt gaactccac aaaggcaac 
      gaccggtcc tgcatgcac atcgagttc gacatcgaa 
      ggctcaaaa atgcgctac gacgccggc gaccacgtc 
      gccgtttac ccaatcaac gactccgac ctggtcgag 
      aaaatcggc aaactgaca aacacggac ctggacacc 
      atcttcacg ctcatcaac accgacgag gagtccagc 
      aagaagcac ccgttcccg tgccccacc acctaccgg 
      accgccctg acccactac ctggacatc accatgaac 
      ccgcgcacc cacgtcctg aaggagctg tccgagtac 
      tgcaccgac cctgcggag aaggagaag ctgaagacc 
      atggcgagc acgtccccc gagggcaaa gccctgtac 
      cagcagtgg gtcaacgaa gacaaccgc aacatcgtg 
      cacatcctc gaagacatg ccctcgtgc aagcccaag 
      cttgaccac ctttgtgaa cttttaccg cgtctccag 
      ccacggtat tactccatt tcttcgtcg cctaagctc 
      taccccaac acagtccac atcacggcg gtcgtggtg 
      gagtacaag acgcccacg gggcgccac aacaagggc 
      gtgtgcacc acctggctg gcgctcaag aaaccggcc 
      ccgggacag gagccccca acagccccc atcttcatc 
      cgcaagtcg cagttcaga ctacccacc aaaacgcag 
      acccccatc attatgatc gggccgggg acgggtctg 
      gcgcccttc cggggcttc atccaggag cgccaccag 
      gccaaggag gaagacaag gctgtgggt gaaacagtt 
      ctctacttt gggtgtcgc aagaaggcc gaggatttt 
      atctacgaa gaggagttg ctcaattac gagaagagc 
      gggctgttg acgctgcat ttggcgttt tcgcgggac 
      caggcgcat aaagtttac gtttcgcat ctgttggag 
      aagaatcgg gaggaggtc tggaggatc attggggag 
      aacaatggg cacttgtat atttgcggg gacgcgaaa 
      tcaatggca ccggaagtg cgtaatatc gtcatgaaa 
      attattcaa gacaaaggt cagatgacc gagcaacag 
      gcgtcagcg tatttgaag aaaatggaa acgcagaaa 
      cgttattcc gccgacgta tggagttaa
    mRNA sequence
    • >TC012437 RA
      ttttaaatg tgccttgat tgctttctg tttcaactc 
      gttcgccaa aatggataa aaaaaatgt gtgcgattg 
      cttgaaatt agttggtgt tttagttat ttcgctact 
      caaaatgga cgaagcaga agcgagtct cggcaccca 
      gcagatccc cgaagcctc cgagtccct cttcagcac 
      aattgacat catcctgct gacgctctt aatcggcgg 
      agtcgcctg gtggttcct aaacaagca gaagaagaa 
      agatgtggc cgccaccgc acgctcata ctccatcca 
      acccacgtc catgaccct ccaaaccag ctccgacaa 
      ctccttcat caaaaaact caagtcgtc gggacgcag 
      cctggtggt gttctacgg gagccagac gggaacggg 
      ggaagagtt cgccgggag gctggccaa ggagggcgt 
      caggtaccg gctgaaggg gatggtggc ggaccccga 
      ggagtgcga catggagga gctggtgaa cttgaaatc 
      gatccccaa cagtctggc tgtgttttg tttggcgac 
      gtacgggga aggtgaccc cactgacaa tgccatgga 
      gttctacga gtggctgca aaacggcga cgctgattt 
      atcaggcct caattatgc ggtgtttgg cttggggaa 
      caaaaccta cgagcacta caaccaagt cgccatcta 
      tgttgacaa gagactaga ggagctggg ggccacgag 
      agtgtttga tctcggact gggcgacga cgacgccaa 
      cattgaaga tgactttat cacgtggaa ggacaagtt 
      ctggcccgc ggtgtgcga acacttcgg catcgagtc 
      aaccggcga ggacgtcag catgcgcca ataccgcct 
      ccaggagtt caaagacga agtcccgga gaagctcta 
      cactggtga gatggcgcg cctccactc cctcaaaaa 
      ccagcggcc gcccttcga cgccaaaaa ccccttcct 
      ggcgaaaat tctggtgaa tcgtgaact ccacaaagg 
      caacgaccg gtcctgcat gcacatcga gttcgacat 
      cgaaggctc aaaaatgcg ctacgacgc cggcgacca 
      cgtcgccgt ttacccaat caacgactc cgacctggt 
      cgagaaaat cggcaaact gacaaacac ggacctgga 
      caccatctt cacgctcat caacaccga cgaggagtc 
      cagcaagaa gcacccgtt cccgtgccc caccaccta 
      ccggaccgc cctgaccca ctacctgga catcaccat 
      gaacccgcg cacccacgt cctgaagga gctgtccga 
      gtactgcac cgaccctgc ggagaagga gaagctgaa 
      gaccatggc gagcacgtc ccccgaggg caaagccct 
      gtaccagca gtgggtcaa cgaagacaa ccgcaacat 
      cgtgcacat cctcgaaga catgccctc gtgcaagcc 
      caagcttga ccacctttg tgaactttt accgcgtct 
      ccagccacg gtattactc catttcttc gtcgcctaa 
      gctctaccc caacacagt ccacatcac ggcggtcgt 
      ggtggagta caagacgcc cacggggcg ccacaacaa 
      gggcgtgtg caccacctg gctggcgct caagaaacc 
      ggccccggg acaggagcc cccaacagc ccccatctt 
      catccgcaa gtcgcagtt cagactacc caccaaaac 
      gcagacccc catcattat gatcgggcc ggggacggg 
      tctggcgcc cttccgggg cttcatcca ggagcgcca 
      ccaggccaa ggaggaaga caaggctgt gggtgaaac 
      agttctcta ctttgggtg tcgcaagaa ggccgagga 
      ttttatcta cgaagagga gttgctcaa ttacgagaa 
      gagcgggct gttgacgct gcatttggc gttttcgcg 
      ggaccaggc gcataaagt ttacgtttc gcatctgtt 
      ggagaagaa tcgggagga ggtctggag gatcattgg 
      ggagaacaa tgggcactt gtatatttg cggggacgc 
      gaaatcaat ggcaccgga agtgcgtaa tatcgtcat 
      gaaaattat tcaagacaa aggtcagat gaccgagca 
      acaggcgtc agcgtattt gaagaaaat ggaaacgca 
      gaaacgtta ttccgccga cgtatggag ttaataaaa 
      cgtttcgtt aggcgtaat tgttgtaaa attaattta 
      aaaattgcc aaggtgccc aaatttgtt gaatttcgg 
      cgacattcc tgctatatt tccaaggac gggttgcta 
      ctgttaaat tttactaaa caatattat atttataat 
      aaatggtat cgttttac
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