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Gene information
General information
TC012045 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7114693..7120467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012045
      iBeetle screen iB_07628

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012045 RA
        MQAMPHQEA DFLRPIPNA QNINSRVPF DGQQMVQYA 
        PGPVQMQGN WGPHQAQMG PQVPQYADK NSHIFNHKE 
        TGLQVPDGQ EISQMQYGE QYNPKIASY YDNYRQPIQ 
        QKNWEQYQY QQEAGNWHP ENETPKNVA YKTTSQMGH 
        SENKENPIH EQEKMAHEL KQHSKEATK QDLKIKIEK 
        HKAKAEHYP DVVAYKMDG SSEDGVPET ASGAVLSLT 
        LGLIITCVM ALLIGCRLR VRRRMRKGG KSYAHDADY 
        LVNGMYL
      CDS sequence
      • >TC012045 RA
        atgcaggcc atgccgcat caggaggcc gattttttg 
        aggccgatt cccaacgcg caaaatatc aattcgcga 
        gtaccgttc gatggtcaa caaatggtg cagtacgcc 
        cctggaccg gtgcaaatg caggggaat tgggggccg 
        catcaggcg cagatgggg ccgcaagtg ccgcaatat 
        gcagataaa aacagccac attttcaat cacaaggag 
        accggcctg caagtgcct gatggccaa gagatcagc 
        caaatgcag tacggggag cagtataac cccaaaatt 
        gccagctat tacgacaat tataggcaa cccatccag 
        caaaaaaac tgggaacaa taccagtac caacaagag 
        gccggaaac tggcaccct gaaaacgag acccccaaa 
        aacgtagct tataaaacc acctcacaa atggggcat 
        tcggaaaac aaagaaaac ccgattcac gagcaagaa 
        aaaatggcc cacgagttg aaacagcac tccaaagaa 
        gcgacgaaa caagacctt aaaataaaa atcgagaaa 
        cacaaggcc aaagccgag cactatccc gacgtggtg 
        gcttacaag atggacgga tcttcggaa gatggcgtc 
        ccggaaacg gcgagcggg gccgtctta tcactaact 
        ctcggctta attatcact tgtgtgatg gcgctgttg 
        atcggttgt cggttgcga gtgcgaaga cggatgcgg 
        aaaggaggc aaaagttat gcccacgac gccgattat 
        ttagtgaac ggaatgtat ttgtga
      mRNA sequence
      • >TC012045 RA
        ctccatccg ttacttcca gggttatcg caacaggaa 
        atctaccga cctcagcta acaccagaa gctttggct 
        cagccccag atgcaggcc atgccgcat caggaggcc 
        gattttttg aggccgatt cccaacgcg caaaatatc 
        aattcgcga gtaccgttc gatggtcaa caaatggtg 
        cagtacgcc cctggaccg gtgcaaatg caggggaat 
        tgggggccg catcaggcg cagatgggg ccgcaagtg 
        ccgcaatat gcagataaa aacagccac attttcaat 
        cacaaggag accggcctg caagtgcct gatggccaa 
        gagatcagc caaatgcag tacggggag cagtataac 
        cccaaaatt gccagctat tacgacaat tataggcaa 
        cccatccag caaaaaaac tgggaacaa taccagtac 
        caacaagag gccggaaac tggcaccct gaaaacgag 
        acccccaaa aacgtagct tataaaacc acctcacaa 
        atggggcat tcggaaaac aaagaaaac ccgattcac 
        gagcaagaa aaaatggcc cacgagttg aaacagcac 
        tccaaagaa gcgacgaaa caagacctt aaaataaaa 
        atcgagaaa cacaaggcc aaagccgag cactatccc 
        gacgtggtg gcttacaag atggacgga tcttcggaa 
        gatggcgtc ccggaaacg gcgagcggg gccgtctta 
        tcactaact ctcggctta attatcact tgtgtgatg 
        gcgctgttg atcggttgt cggttgcga gtgcgaaga 
        cggatgcgg aaaggaggc aaaagttat gcccacgac 
        gccgattat ttagtgaac ggaatgtat ttgtgagag 
        gatattgag catattgtt caaaagtta gttgtaaat 
        agttaggct aatactcat caaaacgat taggtttgt 
        tttcccatc gatgattaa gtgtttaca tcttttgta 
        atcgttttg tatttaatt agagggaaa tatattttc 
        aagtgtccg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY