Status
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Gene information
General information
TC034322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6789439..6794828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034322
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07627

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034322 RA
      MGSYLSEPA TEKESTDDT NGKLTYGAS SMQGWRMTQ 
      EDAHNCILN YDEDTSFFA VYDGHGGQE VAQYCSDKL 
      PDFIKNTPE YKEGNIEEA LVSGFLRFD ALIATPEVV 
      EELRKIADD KDENESDEE ENVNNLYEE AAMPIEQVI 
      EKYTSNLSN PAVKSLKRD IKSPFLTAK TDSDPTVGT 
      SSAGSCSSS TSQGGDGVS SSSEAGLKD KTEEENKPE 
      TNGPGEVSD EKPNSSEVK DEAQAKSDV TSSSTPVEN 
      GTPSKKGKG KAVIKPEPV KTPTNTRPK RTAQQLYKN 
      WLNCGEEES DSSDEEDQT FEGPNEESS EDENEGVNI 
      AIDAEGSEE ESSEEEEEE DEDEEDDDS EDADVRTFK 
      EEPGSDSGC TAVVALLKG NQLYVANAG DSRCIVCRN 
      GKPIEMSID HKPEDKPER KRIQKAGGE VTWEGRVNG 
      GLNLSRAIG DHAYKQNKN LSDREQMIT ALPDIKTLT 
      INPEEDEFM FLACDGIWN AMSNEKVVA FIRPRLQDN 
      TKKLSQICE EMFDHILAP DTVWDNAGS DNMTAIIVK 
      FKSHKRPIS PSQTEESAA KRTKSEAES EAQVDTTV
    CDS sequence
    • >TC034322 RA
      atgggctcg tatttatca gaaccggcc accgagaaa 
      gagtccacc gacgacacc aacggcaaa ttaacttat 
      ggggcaagc tctatgcag ggctggcgt atgacccaa 
      gaggacgca cataactgt atcttaaac tacgacgag 
      gacacttcg tttttcgcc gtctacgac ggacatggg 
      ggccaggaa gtggcccag tattgctcg gacaagctc 
      ccggacttc atcaagaac acgcccgag tacaaggag 
      ggaaacatc gaggaggcg ctcgtttcg ggctttttg 
      aggtttgac gcgctcatt gccacgcca gaagttgtg 
      gaggaattg aggaaaatc gccgatgat aaggacgag 
      aacgagagc gacgaagag gaaaatgtt aacaatttg 
      tacgaagag gcggccatg cccattgag caagttatt 
      gaaaagtac acctctaac ttgtctaat ccggcggtg 
      aaaagttta aaaagggac attaaatcg ccctttttg 
      accgccaaa acagacagt gaccccacg gtaggcacc 
      tcctcagcg ggctcctgc tcgagctcc acgagccaa 
      ggcggcgac ggcgtcagc agctccagc gaggcggga 
      cttaaggat aagacagaa gaggagaac aagccggag 
      accaacggt cctggcgaa gtttccgac gaaaaaccc 
      aacagttca gaggttaaa gacgaagcc caggctaag 
      agtgacgtc acgagttca tcaacacct gttgaaaat 
      ggcacgccg tcaaaaaag ggtaaagga aaggcagtc 
      attaaacca gaacctgta aaaacacca acaaacaca 
      cgaccaaaa cggacggca caacagttg tataaaaac 
      tggttgaat tgtggagag gaagagagt gacagcagc 
      gacgaagaa gaccaaaca tttgagggt ccaaatgag 
      gagagctcg gaggacgaa aatgagggc gttaacatc 
      gcaattgac gcggagggc tctgaggaa gagagttcg 
      gaagaagag gaggaggaa gatgaggac gaggaagac 
      gatgacagt gaagatgca gatgtcaga acctttaaa 
      gaagagcca gggtctgac agtggctgc acggcagtt 
      gtggcgttg ttaaaaggc aaccagttg tacgtagcc 
      aacgccggg gattcccgt tgcatagtg tgtcggaat 
      ggcaaacca atagagatg agtatagac cacaagccg 
      gaggacaag cccgagaga aaaaggata cagaaagca 
      gggggcgaa gtgacatgg gagggccgt gtcaacggc 
      ggcctaaac ctctcacgg gccattgga gaccacgca 
      tacaagcaa aacaagaac ctatcagac cgtgaacaa 
      atgatcaca gctttgccc gacatcaag actttaacg 
      attaatcca gaggaagac gagttcatg tttcttgcg 
      tgcgacgga atctggaat gcgatgtca aacgagaag 
      gtcgtagcc ttcatcagg ccacgtctt caagacaat 
      acgaagaaa ttatcccaa atctgcgaa gagatgttc 
      gaccatatt ctggcacct gacacggtg tgggacaat 
      gccgggagt gacaacatg accgcaatt atcgtaaaa 
      ttcaagtca cacaaaaga ccaatttcg ccttcacag 
      acggaagaa agcgcagcg aaacggaca aagagcgaa 
      gccgaaagt gaggcgcaa gtcgataca acggtttag
    mRNA sequence
    • >TC034322 RA
      atgtcagcc gttttgtgt aaaaattcg tccagcatg 
      gaatcgagt taacaagtc gtaatttag tgccggtat 
      tgtttaatg accaaaaca aaaccacca gcgaatacg 
      tttcgtaat ttgggcaaa aacacaacc aacaatggg 
      ctcgtattt atcagaacc ggccaccga gaaagagtc 
      caccgacga caccaacgg caaattaac ttatggggc 
      aagctctat gcagggctg gcgtatgac ccaagagga 
      cgcacataa ctgtatctt aaactacga cgaggacac 
      ttcgttttt cgccgtcta cgacggaca tgggggcca 
      ggaagtggc ccagtattg ctcggacaa gctcccgga 
      cttcatcaa gaacacgcc cgagtacaa ggagggaaa 
      catcgagga ggcgctcgt ttcgggctt tttgaggtt 
      tgacgcgct cattgccac gccagaagt tgtggagga 
      attgaggaa aatcgccga tgataagga cgagaacga 
      gagcgacga agaggaaaa tgttaacaa tttgtacga 
      agaggcggc catgcccat tgagcaagt tattgaaaa 
      gtacacctc taacttgtc taatccggc ggtgaaaag 
      tttaaaaag ggacattaa atcgccctt tttgaccgc 
      caaaacaga cagtgaccc cacggtagg cacctcctc 
      agcgggctc ctgctcgag ctccacgag ccaaggcgg 
      cgacggcgt cagcagctc cagcgaggc gggacttaa 
      ggataagac agaagagga gaacaagcc ggagaccaa 
      cggtcctgg cgaagtttc cgacgaaaa acccaacag 
      ttcagaggt taaagacga agcccaggc taagagtga 
      cgtcacgag ttcatcaac acctgttga aaatggcac 
      gccgtcaaa aaagggtaa aggaaaggc agtcattaa 
      accagaacc tgtaaaaac accaacaaa cacacgacc 
      aaaacggac ggcacaaca gttgtataa aaactggtt 
      gaattgtgg agaggaaga gagtgacag cagcgacga 
      agaagacca aacatttga gggtccaaa tgaggagag 
      ctcggagga cgaaaatga gggcgttaa catcgcaat 
      tgacgcgga gggctctga ggaagagag ttcggaaga 
      agaggagga ggaagatga ggacgagga agacgatga 
      cagtgaaga tgcagatgt cagaacctt taaagaaga 
      gccagggtc tgacagtgg ctgcacggc agttgtggc 
      gttgttaaa aggcaacca gttgtacgt agccaacgc 
      cggggattc ccgttgcat agtgtgtcg gaatggcaa 
      accaataga gatgagtat agaccacaa gccggagga 
      caagcccga gagaaaaag gatacagaa agcaggggg 
      cgaagtgac atgggaggg ccgtgtcaa cggcggcct 
      aaacctctc acgggccat tggagacca cgcatacaa 
      gcaaaacaa gaacctatc agaccgtga acaaatgat 
      cacagcttt gcccgacat caagacttt aacgattaa 
      tccagagga agacgagtt catgtttct tgcgtgcga 
      cggaatctg gaatgcgat gtcaaacga gaaggtcgt 
      agccttcat caggccacg tcttcaaga caatacgaa 
      gaaattatc ccaaatctg cgaagagat gttcgacca 
      tattctggc acctgacac ggtgtggga caatgccgg 
      gagtgacaa catgaccgc aattatcgt aaaattcaa 
      gtcacacaa aagaccaat ttcgccttc acagacgga 
      agaaagcgc agcgaaacg gacaaagag cgaagccga 
      aagtgaggc gcaagtcga tacaacggt ttagatttc 
      tttattttt aaaaacggt tttttaatg aaaggtgac 
      gagatcatt ttcagtttt atgctgtca ttgtctata 
      tcttttgtt atcaacacc atggtgtag gtgccttct 
      gttaaaatg agcactgtg gtggtaatt tttcataga 
      tttgtataa tactgctgt attttttat tatgtaagg 
      ttaattttt taatcccgg aagtcattt tttaattgt 
      ctggggaac tgtcgattg taaacctct gtagcctat 
      ttaccggta agtttgtgt atttatttt tcattgcga 
      ttcagttcg tgtaataaa acaatgccg caaacgtaa 
      gtatcattt aaccaattt tatccatta cgtgggcca 
      gtaagccag aaacgaggt ttttagaaa gactgaaat 
      aattccatt cgcagtttt gttttgata tttattttt 
      gtaactggt tttctcgct ctagggact atgaaaagt 
      tgaataaaa gcagttgca aataaacac tttattttt 
      attagagta catagttta tcaaagatc tgtgagaaa 
      caatgcgac aacaaaaac tgttacacg attagtttc 
      ttcttttgt aatttaata aagtacaat aaataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY