Status
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Gene information
General information
TC010962 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3411604..3414929
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010962
    iBeetle screen iB_07609

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010962 RA
      MAMIIFICV HFDYITTTA IGLFIIAFL FQTYITRRN 
      FNNRNFIVQ KTDSRIRLL NEIICGIRA IKLYVWENV 
      FAKWMYTAR KQELDAISK ANFLRLTVW TFTVYVQKI 
      ALYICVLIK TLLGFQISA AYVFTVANI TELTRTTLH 
      ILSSSLIFT TEGIVSLKQ IEEFLLSNT YKKRQQEPP 
      VTFSCILEN VFLENCLYN VCLNIAPKE LLGLTGVCG 
      SGKTSLLKT ILGEVEVKG TIFCQGTIS YAPQEPWIF 
      SGSLEENVL FGEKFDKER YTKVIQVCA LEHDLSLLP 
      NGENTLVGE RGVMLSGGQ RARIGLARA VYRNADLYL 
      LDDSLSAVD NLVATHIFN NCIRNFLSD KSIIFVTTQ 
      TSFLKQVDR VCLLSEGKV EALGHYNEV EDLVDKSVK 
      EIAQPVIQS NDTLLEIKE NIGNTNTSP YKSYFKAGG 
      FTRTILLFL FFVFVQVQV NSMDCLLVF WINLKESTF 
      RLNTKIFLF TCGAISISV VVSTHLSSY ALTKYCKRA 
      SRCLHNALF EKLLKGSMT FFNNHSSGR ILNRFSTDV 
      GTVDEIVPL SLGEMIRKF LYILGCYII IIYVNFWMV 
      IPTIIFISL VALYVLLFY PVISRVKHF EGLKRSPII 
      TQLSASIQG LEIIRAAKA QTFLQNKFD EIQNLHSAA 
      FYMHKALYF TFCFWTDFT QFLYTAVVI CFFVLYGNE 
      MIVGNVGLA ITQSLIFIG LTQYILKMW ANLNSEMVS 
      VQRIVEYSD LALEADEGF LTPPGVWPE KGNIQFNSV 
      SMRYSSNAP LVLKNINLH VEGGSKIGI VGRTGSGKS 
      SLISILFRL FPFEGSIRI DGIDTKFLP LNILRSNIA 
      IIPQEPILF CGSIRKNLD PFNEFDNPH LWSALEEVQ 
      LKNVVRNLD SEINLSVGQ KQLLCLARA VLKKSKIIA 
      LDEATSSVD VETDHLIQS TIRRRFQDS TVLTIAHRL 
      DTVMDCDRI LVLESGRMV EYGKTEELL KNSEGYFYK 
      YVHAS
    CDS sequence
    • >TC010962 RA
      atggcaatg ataatcttc atatgtgta cattttgat 
      tacataacc actactgca attggatta tttattata 
      gctttctta tttcaaact tatattact agaagaaat 
      tttaacaat cgtaatttc attgtgcag aaaactgat 
      tctaggatt cgtttgcta aatgaaatc atttgtgga 
      attcgagca attaaattg tacgtatgg gagaatgta 
      tttgcaaaa tggatgtac acagccaga aagcaagag 
      ctcgatgca atttcaaaa gcaaatttt cttcgtttg 
      acagtttgg actttcacc gtatatgtg caaaaaatt 
      gccctttac atttgcgtt ttaattaaa acattattg 
      ggcttccag atatcagct gcatatgtt tttaccgtc 
      gccaatatc actgaatta acaagaaca acgttacat 
      attttaagc agctcactt atttttacg acagaaggc 
      atagtttca cttaagcaa atagaagaa tttttactt 
      tctaataca tacaaaaaa cgacaacaa gaaccacct 
      gtaactttt agctgcata cttgaaaac gtatttctt 
      gaaaactgt ttatacaat gtctgtttg aatatagca 
      ccaaaggag ttattaggt cttaccgga gtgtgtgga 
      agtggtaaa acttctcta ctaaaaact atacttgga 
      gaagttgaa gtcaaagga accattttt tgtcaagga 
      acaatttct tacgctcca caagaacca tggatcttt 
      tccggttct ctcgaggaa aatgtttta tttggagag 
      aaattcgat aaagagagg tatacgaaa gtaattcaa 
      gtttgtgct ttagaacac gatttatct cttttacca 
      aacggtgaa aacacctta gttggagaa cgaggtgta 
      atgttaagc gggggccaa agagctaga ataggtcta 
      gcacgagct gtttataga aatgcagat ttgtatctt 
      cttgatgat tctttatca gctgtggat aacttagtt 
      gcaactcac attttcaat aactgtata agaaacttt 
      ttgagtgac aaaagcatt atttttgta actactcaa 
      accagtttc ttgaaacag gtagataga gtgtgtctt 
      ttgtcagaa ggaaaagtt gaagccctt ggacattac 
      aacgaagtt gaagacttg gttgataaa agtgtgaaa 
      gaaatagca caaccagtt attcaaagt aatgacaca 
      ttattagag ataaaagaa aatattggt aataccaat 
      acaagtcct tacaaaagt tatttcaaa gcgggaggt 
      ttcacgcgt actatttta ctattttta ttttttgtt 
      tttgtgcaa gtacaggta aattcaatg gattgtttg 
      ttagtcttt tggattaat ttaaaagag agtacattc 
      agattaaat accaaaatt ttcttgttc acttgtggt 
      gcaatttcg atcagtgta gtggtatcc actcattta 
      agttcttat gcccttaca aaatactgc aaaagagct 
      tcaagatgt ttacacaac gcactattt gaaaagtta 
      ctcaaaggt tctatgact tttttcaat aatcattct 
      tcggggaga attctcaat agattttcg accgatgtt 
      ggaacagtt gacgaaatt gttcctcta agtttagga 
      gaaatgata agaaaattt ttatacatt cttggctgt 
      tatatcatt atcatctat gttaatttc tggatggta 
      atcccgact ataatattt atttctctt gttgcactt 
      tacgtcttg ttattttat cctgttata agtcgagtc 
      aaacatttt gaaggattg aaaagaagt ccaatcatc 
      acacagtta tcagcttca atccaaggt ctggaaatt 
      atcagagca gccaaagcc caaacattt ctacaaaat 
      aaatttgac gaaatacaa aatctgcac agtgccgcc 
      ttttacatg cataaagct ctatatttt acattttgt 
      ttttggact gactttact caattttta tacactgct 
      gtagtaata tgctttttc gtactgtac ggtaacgaa 
      atgattgta ggaaacgtt ggcttggca ataacgcag 
      tcactaata tttattgga ttgacacag tatattcta 
      aaaatgtgg gcaaattta aattcggag atggtttca 
      gtgcaacgt attgtcgag tattctgac ttagcttta 
      gaagcagat gaaggattt ttaacaccg ccgggggtg 
      tggccagag aaaggaaat attcaattt aattcggtt 
      tcaatgaga tattcttca aatgcgccg ttggtcctc 
      aaaaacatt aaccttcat gtagaaggt ggctcgaaa 
      attggaatt gttggaagg accggttct ggcaaatcg 
      tctctaatt tcaatccta tttcgttta tttccgttc 
      gaaggtagt atccgcatt gacggaatt gatacgaaa 
      ttcttacct ctaaacatc ctacgttcc aatattgcc 
      atcattcct caagaaccg attttattc tgcggttca 
      atccgaaaa aatttagac ccttttaat gagtttgac 
      aatccccac ttgtggtca gctttggaa gaagtgcaa 
      ctaaaaaat gttgttcga aatctcgac tccgagatt 
      aatttgagt gttggtcaa aagcaactg ctttgtcta 
      gcacgtgca gtgttaaag aagtcgaaa attattgct 
      cttgatgaa gctacatct agtgttgat gttgaaact 
      gatcatttg attcagtcg acgattagg agaagattt 
      caggattcc accgtacta acgattgct catcgactg 
      gatactgtt atggattgt gatagaatt ttggttttg 
      gagtctgga agaatggtg gagtatgga aaaacggaa 
      gagctacta aaaaattcg gagggatac ttctacaaa 
      tacgttcat gctagttga
    mRNA sequence
    • >TC010962 RA
      aatctacac ttgaaaaaa tgacccacg gacgacttg 
      ttaatcttt tatcgaatc acgttgaaa aatttgatc 
      ggacagcaa tatatttcc atttcatct ggatggggt 
      gtctaaaag ctatggcaa tgataatct tcatatgtg 
      tacattttg attacataa ccactactg caattggat 
      tatttatta tagctttct tatttcaaa cttatatta 
      ctagaagaa attttaaca atcgtaatt tcattgtgc 
      agaaaactg attctagga ttcgtttgc taaatgaaa 
      tcatttgtg gaattcgag caattaaat tgtacgtat 
      gggagaatg tatttgcaa aatggatgt acacagcca 
      gaaagcaag agctcgatg caatttcaa aagcaaatt 
      ttcttcgtt tgacagttt ggactttca ccgtatatg 
      tgcaaaaaa ttgcccttt acatttgcg ttttaatta 
      aaacattat tgggcttcc agatatcag ctgcatatg 
      tttttaccg tcgccaata tcactgaat taacaagaa 
      caacgttac atattttaa gcagctcac ttattttta 
      cgacagaag gcatagttt cacttaagc aaatagaag 
      aatttttac tttctaata catacaaaa aacgacaac 
      aagaaccac ctgtaactt ttagctgca tacttgaaa 
      acgtatttc ttgaaaact gtttataca atgtctgtt 
      tgaatatag caccaaagg agttattag gtcttaccg 
      gagtgtgtg gaagtggta aaacttctc tactaaaaa 
      ctatacttg gagaagttg aagtcaaag gaaccattt 
      tttgtcaag gaacaattt cttacgctc cacaagaac 
      catggatct tttccggtt ctctcgagg aaaatgttt 
      tatttggag agaaattcg ataaagaga ggtatacga 
      aagtaattc aagtttgtg ctttagaac acgatttat 
      ctcttttac caaacggtg aaaacacct tagttggag 
      aacgaggtg taatgttaa gcgggggcc aaagagcta 
      gaataggtc tagcacgag ctgtttata gaaatgcag 
      atttgtatc ttcttgatg attctttat cagctgtgg 
      ataacttag ttgcaactc acattttca ataactgta 
      taagaaact ttttgagtg acaaaagca ttatttttg 
      taactactc aaaccagtt tcttgaaac aggtagata 
      gagtgtgtc ttttgtcag aaggaaaag ttgaagccc 
      ttggacatt acaacgaag ttgaagact tggttgata 
      aaagtgtga aagaaatag cacaaccag ttattcaaa 
      gtaatgaca cattattag agataaaag aaaatattg 
      gtaatacca atacaagtc cttacaaaa gttatttca 
      aagcgggag gtttcacgc gtactattt tactatttt 
      tattttttg tttttgtgc aagtacagg taaattcaa 
      tggattgtt tgttagtct tttggatta atttaaaag 
      agagtacat tcagattaa ataccaaaa ttttcttgt 
      tcacttgtg gtgcaattt cgatcagtg tagtggtat 
      ccactcatt taagttctt atgccctta caaaatact 
      gcaaaagag cttcaagat gtttacaca acgcactat 
      ttgaaaagt tactcaaag gttctatga cttttttca 
      ataatcatt cttcgggga gaattctca atagatttt 
      cgaccgatg ttggaacag ttgacgaaa ttgttcctc 
      taagtttag gagaaatga taagaaaat ttttataca 
      ttcttggct gttatatca ttatcatct atgttaatt 
      tctggatgg taatcccga ctataatat ttatttctc 
      ttgttgcac tttacgtct tgttatttt atcctgtta 
      taagtcgag tcaaacatt ttgaaggat tgaaaagaa 
      gtccaatca tcacacagt tatcagctt caatccaag 
      gtctggaaa ttatcagag cagccaaag cccaaacat 
      ttctacaaa ataaatttg acgaaatac aaaatctgc 
      acagtgccg ccttttaca tgcataaag ctctatatt 
      ttacatttt gtttttgga ctgacttta ctcaatttt 
      tatacactg ctgtagtaa tatgctttt tcgtactgt 
      acggtaacg aaatgattg taggaaacg ttggcttgg 
      caataacgc agtcactaa tatttattg gattgacac 
      agtatattc taaaaatgt gggcaaatt taaattcgg 
      agatggttt cagtgcaac gtattgtcg agtattctg 
      acttagctt tagaagcag atgaaggat ttttaacac 
      cgccggggg tgtggccag agaaaggaa atattcaat 
      ttaattcgg tttcaatga gatattctt caaatgcgc 
      cgttggtcc tcaaaaaca ttaaccttc atgtagaag 
      gtggctcga aaattggaa ttgttggaa ggaccggtt 
      ctggcaaat cgtctctaa tttcaatcc tatttcgtt 
      tatttccgt tcgaaggta gtatccgca ttgacggaa 
      ttgatacga aattcttac ctctaaaca tcctacgtt 
      ccaatattg ccatcattc ctcaagaac cgattttat 
      tctgcggtt caatccgaa aaaatttag accctttta 
      atgagtttg acaatcccc acttgtggt cagctttgg 
      aagaagtgc aactaaaaa atgttgttc gaaatctcg 
      actccgaga ttaatttga gtgttggtc aaaagcaac 
      tgctttgtc tagcacgtg cagtgttaa agaagtcga 
      aaattattg ctcttgatg aagctacat ctagtgttg 
      atgttgaaa ctgatcatt tgattcagt cgacgatta 
      ggagaagat ttcaggatt ccaccgtac taacgattg 
      ctcatcgac tggatactg ttatggatt gtgatagaa 
      ttttggttt tggagtctg gaagaatgg tggagtatg 
      gaaaaacgg aagagctac taaaaaatt cggagggat 
      acttctaca aatacgttc atgctagtt gattgatta 
      cttgttgac gtttaacac aaaaaaact ggtgaaaaa 
      gtcatggtg taataagaa taaatgatc taaatttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY