Status
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Gene information
General information
TC012312 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4044972..4047394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012312
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07602

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012312 RA
      MAQQLETKI QEWLKWDRN EKTSSEIKK LAQEKNYDE 
      LSKILLDRL SFGTAGLRG RMGPGYAAM NDLVIIQTG 
      QGLLKYLEH CDKDLLHRN GIVIGYDGR HNSRRWAEL 
      TAAIFLHAG HPVRLFSKI VPTPFVPFA VSKYKSAIG 
      VMVTASHNP KEDNGYKVY GPNGSQIIS PVDKDIQRN 
      ILESLVPLD SSWDTTVLK SGLLTDPLS ETLRDYLNV 
      INDTILPQH RELNKKAEV LFTYTAMHG VGYNYIRQV 
      FDLIGVTMV PVEEQKDPH PDFPTVKFP NPEEGKSSL 
      DLSFRTANE NGSRVIIAN DPDADRMAA AEKNAKTGE 
      WKVFTGNEL GALLGWWCL FCFKTKYPR EDLQNTYML 
      SSTVSSMIL RSMSRKEGF NFIETLTGF KWMGNKSYE 
      LLKQGKKVI FAFEEAIGF CCGTAVLDK DGVSAAFQL 
      ATLTSYLAM QNKSLTDKL EEIYAEYGY HVSLNSYFI 
      CHDGEKIKR IFDRMRNLN GKAYPSAIL NNKYQITTI 
      RDLTTGYDN GQPDNKAIL PVSTSSQMI TFNFSNGLV 
      CTLRTSGTE PKVKYYTEM CAEPGNTNR EGVVATLKE 
      MVDAIVEEF LQPVQNELI PKSD
    CDS sequence
    • >TC012312 RA
      atggcccaa cagctcgaa acgaaaatc caggagtgg 
      ttgaaatgg gaccggaac gagaaaacc tcctccgaa 
      atcaaaaaa ctggcacag gagaagaat tacgacgaa 
      ctgtcgaaa attctcctc gatcggctg tctttcggg 
      accgccggt ctgaggggc cgaatgggc ccaggttac 
      gccgctatg aacgaccta gttataatc caaacgggt 
      caagggctg cttaagtac ttagagcac tgtgataag 
      gatttgttg cacaggaac gggattgtg attggttac 
      gacggacgg cataacagc agaaggtgg gccgaacta 
      accgcggcg attttccta catgctggc caccctgtg 
      cggcttttt agcaagata gtaccgact ccatttgta 
      ccatttgcc gtctcgaag tataaaagt gcgataggg 
      gtgatggtc acagcttcg cataaccca aaagaggat 
      aatggatat aaggtttat ggtccgaac ggatcgcaa 
      attatctcg ccggtggat aaggacatc cagagaaat 
      attctagag agtttggta ccactggac tcgtcatgg 
      gacacaaca gtattaaaa agtggcctt ctaacagac 
      cctctcagt gaaactttg agagactat ttgaatgtg 
      atcaacgac acgattttg ccgcagcat cgcgagctt 
      aataagaag gccgaggta cttttcacc tacactgca 
      atgcacggt gttggttat aattacatt aggcaagtt 
      ttcgacttg attggtgtg acaatggta ccagtggag 
      gagcaaaaa gacccccat cccgacttt ccgacagta 
      aaattccct aatccagag gagggcaaa agtagctta 
      gatctttca tttcggacg gcaaacgaa aatgggagc 
      cgtgtgatc atagcgaac gacccagat gctgaccgc 
      atggccgct gctgagaaa aatgcaaag actggtgag 
      tggaaagtt ttcacgggg aacgagctg ggggccctt 
      ttgggttgg tggtgtttg ttttgcttc aaaaccaag 
      taccccaga gaggatttg caaaacacc tacatgctt 
      agtagtacc gtaagttcg atgattttg cgctccatg 
      tccaggaag gaagggttc aacttcatc gagactttg 
      actgggttc aaatggatg gggaacaag tcgtacgag 
      ctgttgaag caagggaag aaagtgatt tttgcgttt 
      gaggaagct atcggtttc tgctgtggt actgctgtt 
      ctggataag gacggcgtt tcggcggct tttcaactt 
      gccactttg acttcgtac ttagcgatg caaaacaag 
      tctctgact gataaattg gaggagatt tatgccgaa 
      tatgggtac cacgtgtcg ctcaattcg tattttatt 
      tgtcacgat ggggagaag attaaaagg attttcgac 
      cgcatgagg aatctgaac gggaaggcg tacccgtct 
      gccattctt aataataag taccaaatt accacaatt 
      agggatttg accactggc tatgataat ggccaacct 
      gacaataaa gccatcttg ccggtgtct acaagcagc 
      caaatgata acgttcaat ttttcaaac gggcttgtt 
      tgcacgctt aggaccagc ggaactgaa ccgaaagtc 
      aaatattac acggaaatg tgcgccgaa ccgggaaat 
      acaaatcgg gaaggtgtt gtggcgact ttaaaggaa 
      atggtcgat gcgatagtt gaggagttt ttgcaaccg 
      gttcaaaat gaactaatt ccaaagtcg gactaa
    mRNA sequence
    • >TC012312 RA
      tagcaagtg acgcttcag tgcagatgg ttttttatt 
      tttagtagt tatctacct ttcttaatg attcactca 
      ggcgggttt ctaattctt gcaacatat ttttaaaca 
      aatatctat tttccatta aataatacg tttcccaat 
      cgtgttgat aattttcaa aattctgac cccactcga 
      atcgaaaac atctcctta tcaatcagt gatgcaaca 
      ttcgtatgt ggatcagta tcgatttca cactattac 
      agtgagcat tccgttgca gtgaacaat ggcccaaca 
      gctcgaaac gaaaatcca ggagtggtt gaaatggga 
      ccggaacga gaaaacctc ctccgaaat caaaaaact 
      ggcacagga gaagaatta cgacgaact gtcgaaaat 
      tctcctcga tcggctgtc tttcgggac cgccggtct 
      gaggggccg aatgggccc aggttacgc cgctatgaa 
      cgacctagt tataatcca aacgggtca agggctgct 
      taagtactt agagcactg tgataagga tttgttgca 
      caggaacgg gattgtgat tggttacga cggacggca 
      taacagcag aaggtgggc cgaactaac cgcggcgat 
      tttcctaca tgctggcca ccctgtgcg gctttttag 
      caagatagt accgactcc atttgtacc atttgccgt 
      ctcgaagta taaaagtgc gataggggt gatggtcac 
      agcttcgca taacccaaa agaggataa tggatataa 
      ggtttatgg tccgaacgg atcgcaaat tatctcgcc 
      ggtggataa ggacatcca gagaaatat tctagagag 
      tttggtacc actggactc gtcatggga cacaacagt 
      attaaaaag tggccttct aacagaccc tctcagtga 
      aactttgag agactattt gaatgtgat caacgacac 
      gattttgcc gcagcatcg cgagcttaa taagaaggc 
      cgaggtact tttcaccta cactgcaat gcacggtgt 
      tggttataa ttacattag gcaagtttt cgacttgat 
      tggtgtgac aatggtacc agtggagga gcaaaaaga 
      cccccatcc cgactttcc gacagtaaa attccctaa 
      tccagagga gggcaaaag tagcttaga tctttcatt 
      tcggacggc aaacgaaaa tgggagccg tgtgatcat 
      agcgaacga cccagatgc tgaccgcat ggccgctgc 
      tgagaaaaa tgcaaagac tggtgagtg gaaagtttt 
      cacggggaa cgagctggg ggccctttt gggttggtg 
      gtgtttgtt ttgcttcaa aaccaagta ccccagaga 
      ggatttgca aaacaccta catgcttag tagtaccgt 
      aagttcgat gattttgcg ctccatgtc caggaagga 
      agggttcaa cttcatcga gactttgac tgggttcaa 
      atggatggg gaacaagtc gtacgagct gttgaagca 
      agggaagaa agtgatttt tgcgtttga ggaagctat 
      cggtttctg ctgtggtac tgctgttct ggataagga 
      cggcgtttc ggcggcttt tcaacttgc cactttgac 
      ttcgtactt agcgatgca aaacaagtc tctgactga 
      taaattgga ggagattta tgccgaata tgggtacca 
      cgtgtcgct caattcgta ttttatttg tcacgatgg 
      ggagaagat taaaaggat tttcgaccg catgaggaa 
      tctgaacgg gaaggcgta cccgtctgc cattcttaa 
      taataagta ccaaattac cacaattag ggatttgac 
      cactggcta tgataatgg ccaacctga caataaagc 
      catcttgcc ggtgtctac aagcagcca aatgataac 
      gttcaattt ttcaaacgg gcttgtttg cacgcttag 
      gaccagcgg aactgaacc gaaagtcaa atattacac 
      ggaaatgtg cgccgaacc gggaaatac aaatcggga 
      aggtgttgt ggcgacttt aaaggaaat ggtcgatgc 
      gatagttga ggagttttt gcaaccggt tcaaaatga 
      actaattcc aaagtcgga ctaaaacat tccatttgt 
      tgtcttgca ccatagatt atagaattc taaaatgtg 
      atcacttga tatacttaa aatgacatt tttgtatgt 
      attgtttta aaatataca ccagttagt agg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY