| General information | |||
| TC012164 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 4004903..4008500 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012164 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07601 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012164 RA
MNYGPLISK ILLSFILAS TVLYRYGNW FRHHIVVTL IVLLAWYFS FLIIFALPL DVISTVYRQ CKEEHDTFD NATSSLMQN VTNKSIEKC EEPWSRVPH EVFPNLWRT VYWSTQCLT WLVMPMMQS YIKAGDFTV KGKLKSAVI DNAIYYGSY LLICGVLLI YLALQPGIH LDWSKLKAI ASSASNTWG LFLLVLLFG YALVEVPRT LWNNSNHSF VLTHSYFKA AKLSSDKCE AEETVDDVL ESLQAVSLA INSRHPLHA HLETILQKV PTELRDRMN RRQLPEDTP TDLPSEKAL VRLHKQTIK SLQVFQRTE TQWNLLVEK IFDLEDTIK NQISRDKRF KRTFQKPRS LFRRFLYVP TVEWYWKCL FYGYTQKLL AVMAGLFSV AVVWSEVTF FNKQPVLSI FAAIVNRAK VKYDYFTIE LLSTIIILY LCYCAYSTV LKIKLLNLY YLAPHHQTN EYSLIFSGM MLSRLTPPL CLNFLGLIH MDSHIIKKQ VMETHYTQI MGHMDVISI ISDGFNVYF PMAILVFCL ATYFSVGSR LLSMLGFHQ FVGDDEMTT DLIEEGREL IKREKRKRQ RSEEASTRR TEFSERFPT TGRYRPRGS TDTIRPMTQ LENLDNTRS NLLQESHPL DSYESRRYT SEVDTRFPP DQDDIDARF GASTRINER YNSSQELEP RFADSYQSD GNYRNRVGA PPRGIFDDV
- >TC012164 RA
atgaattac gggccgctg atttcgaaa attttgctc tcttttatt cttgcctcg acggtcttg taccgatat gggaactgg ttcaggcac cacattgtg gtgaccttg attgtgctt cttgcttgg tatttcagt ttcttgatt attttcgcg cttccgttg gacgtaatt tcgacggtt taccgccag tgtaaggaa gaacatgac acttttgac aacgccact tcttcactg atgcaaaat gttacaaat aaaagtatt gaaaagtgt gaggagccg tggagtcga gtccctcat gaggttttt ccgaacttg tggcgtact gtgtattgg agcactcag tgcttaact tggctagtc atgcccatg atgcaatcg tacatcaaa gctggtgat tttacagtc aaaggcaaa ttgaaatcg gctgtaatc gacaatgcg atttattac ggctcttat ttgctaatc tgtggagtt ctactaatt tacttggcc ctccagcct gggattcac ctcgactgg tcaaaacta aaagcaatt gcttcgtca gctagcaat acttggggc ttattcctc ctagttttg ctgttcgga tacgcttta gttgaagtc cctcgaact ctgtggaac aactcaaac cacagtttt gtgctaaca cattcgtat ttcaaggcc gccaagctg agttcggac aagtgcgag gccgaggaa accgtcgac gatgtcctc gagtcgcta caagccgtc tcattggcc attaattcg cggcatcca ttgcacgcc cacttggag accattctg caaaaggtg cccacggaa ttacgggac agaatgaac cggaggcag ctccccgaa gacaccccc acggattta ccgagcgaa aaggcacta gtcaggctc cacaagcaa acgataaag tcgctccag gtgtttcag agaaccgaa acacagtgg aaccttctc gtcgagaag attttcgat cttgaggac actatcaaa aatcagatt tcgcgagat aagcgattt aaacgcaca ttccaaaaa ccccgatct cttttcagg aggtttttg tacgtcccg acagttgag tggtactgg aagtgtctt ttttatggc tacacgcaa aaattattg gctgttatg gctgggctt ttttcggtg gctgtggtt tggtccgaa gtgactttt ttcaacaaa cagccagtt ctctcgatt tttgccgct attgttaac agagctaaa gtcaagtac gactatttt acgatagag ttattgtcg accataata atcctgtac ttatgctac tgtgcttac tcaacggtc ctcaaaata aaactcctc aacttgtat tacttagca ccgcaccac caaacgaac gagtacagt ttaatcttc tcagggatg atgttgagc cgactgacg cctcctctg tgtttaaat ttcctcggc ttaattcac atggacagc cacataata aaaaaacaa gtcatggag acccactac acccaaatc atgggtcat atggacgtc atttcaata atctccgac ggcttcaac gtttacttc ccaatggcc attctagtc ttttgttta gcgacgtat ttcagcgtc ggctcgcgc cttttatca atgctgggt ttccaccag ttcgtcggc gacgacgaa atgaccacc gacctgatc gaagaaggc cgcgaattg atcaagcgg gaaaaacgc aaacgccag cggtcggag gaggccagc acgagacga accgaattc agtgaacgg ttcccgacc acggggagg tacaggccc cggggaagc accgacacg attcgtcca atgacgcaa ctggaaaat ttggacaac acgcgctca aatttgctg caagagtcg cacccgctg gactcgtac gagagtcgg aggtacacg agtgaggtc gatacgagg tttcccccg gatcaggac gatatcgat gcgcggttc ggcgccagc acgaggatt aacgaacgg tataacagt tcgcaggaa ctggagccg aggtttgcc gactcgtat cagtcggat ggtaactat agaaaccgc gtgggggcg cccccgagg ggcattttc gacgacgtt taa
- >TC012164 RA
ttaataatt aagtcaaat gtgatgatt tgggcgaaa attggcgca aattattgg tgataaccc agtaatcaa tattggcca cattgccaa ctgacactt gtcaaagtg acgtttcgt gtgtgtgtg tgtgaaggt tcaatattt tcggaattt tcacgtgcg gattaattt tccggccac aggaaatct ctaattact tggacgatc gatattgat gactatgtt gtgatgtca tttagtgac aatgtccag tgtggaaaa accaaaacc gattttgcc taagaaagg acaaatcat cattgaaga catccagga cttggtgtt gtgcaaacc ccgtctgat cccccttaa gagtttcac tttggaaaa attggagaa aatgcaaca agaggccca aaaatcgca atcccagca gagtcccaa agacgagta aaaatgaat tacgggccg ctgatttcg aaaattttg ctctctttt attcttgcc tcgacggtc ttgtaccga tatgggaac tggttcagg caccacatt gtggtgacc ttgattgtg cttcttgct tggtatttc agtttcttg attattttc gcgcttccg ttggacgta atttcgacg gtttaccgc cagtgtaag gaagaacat gacactttt gacaacgcc acttcttca ctgatgcaa aatgttaca aataaaagt attgaaaag tgtgaggag ccgtggagt cgagtccct catgaggtt tttccgaac ttgtggcgt actgtgtat tggagcact cagtgctta acttggcta gtcatgccc atgatgcaa tcgtacatc aaagctggt gattttaca gtcaaaggc aaattgaaa tcggctgta atcgacaat gcgatttat tacggctct tatttgcta atctgtgga gttctacta atttacttg gccctccag cctgggatt cacctcgac tggtcaaaa ctaaaagca attgcttcg tcagctagc aatacttgg ggcttattc ctcctagtt ttgctgttc ggatacgct ttagttgaa gtccctcga actctgtgg aacaactca aaccacagt tttgtgcta acacattcg tatttcaag gccgccaag ctgagttcg gacaagtgc gaggccgag gaaaccgtc gacgatgtc ctcgagtcg ctacaagcc gtctcattg gccattaat tcgcggcat ccattgcac gcccacttg gagaccatt ctgcaaaag gtgcccacg gaattacgg gacagaatg aaccggagg cagctcccc gaagacacc cccacggat ttaccgagc gaaaaggca ctagtcagg ctccacaag caaacgata aagtcgctc caggtgttt cagagaacc gaaacacag tggaacctt ctcgtcgag aagattttc gatcttgag gacactatc aaaaatcag atttcgcga gataagcga tttaaacgc acattccaa aaaccccga tctcttttc aggaggttt ttgtacgtc ccgacagtt gagtggtac tggaagtgt cttttttat ggctacacg caaaaatta ttggctgtt atggctggg cttttttcg gtggctgtg gtttggtcc gaagtgact tttttcaac aaacagcca gttctctcg atttttgcc gctattgtt aacagagct aaagtcaag tacgactat tttacgata gagttattg tcgaccata ataatcctg tacttatgc tactgtgct tactcaacg gtcctcaaa ataaaactc ctcaacttg tattactta gcaccgcac caccaaacg aacgagtac agtttaatc ttctcaggg atgatgttg agccgactg acgcctcct ctgtgttta aatttcctc ggcttaatt cacatggac agccacata ataaaaaaa caagtcatg gagacccac tacacccaa atcatgggt catatggac gtcatttca ataatctcc gacggcttc aacgtttac ttcccaatg gccattcta gtcttttgt ttagcgacg tatttcagc gtcggctcg cgcctttta tcaatgctg ggtttccac cagttcgtc ggcgacgac gaaatgacc accgacctg atcgaagaa ggccgcgaa ttgatcaag cgggaaaaa cgcaaacgc cagcggtcg gaggaggcc agcacgaga cgaaccgaa ttcagtgaa cggttcccg accacgggg aggtacagg ccccgggga agcaccgac acgattcgt ccaatgacg caactggaa aatttggac aacacgcgc tcaaatttg ctgcaagag tcgcacccg ctggactcg tacgagagt cggaggtac acgagtgag gtcgatacg aggtttccc ccggatcag gacgatatc gatgcgcgg ttcggcgcc agcacgagg attaacgaa cggtataac agttcgcag gaactggag ccgaggttt gccgactcg tatcagtcg gatggtaac tatagaaac cgcgtgggg gcgcccccg aggggcatt ttcgacgac gtttaagtt gtttaagtc agtgatgtt tattatatt gcgttgaga aattatatt tttatattg tttataagg cagtttggt ttttcaggt ttcagctga aacccaccg tagtcaagt cttgtaata gtaggtttt agcgtccaa cgtttcacg gtagtaact tatttcagt gtaatcacg atttcttct gggtcggat ttttaaatc aagttggca acactaaat aatgagtcg caggccact ttgatgaga aacttaatt gaaagcctg gcatttgtt atgtaatat tccaattac acgtgtaat aaaa
