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Gene information
General information
TC012164 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4004903..4008500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012164
    iBeetle screen iB_07601

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012164 RA
      MNYGPLISK ILLSFILAS TVLYRYGNW FRHHIVVTL 
IVLLAWYFS FLIIFALPL DVISTVYRQ CKEEHDTFD 
NATSSLMQN VTNKSIEKC EEPWSRVPH EVFPNLWRT 
VYWSTQCLT WLVMPMMQS YIKAGDFTV KGKLKSAVI 
DNAIYYGSY LLICGVLLI YLALQPGIH LDWSKLKAI 
ASSASNTWG LFLLVLLFG YALVEVPRT LWNNSNHSF 
VLTHSYFKA AKLSSDKCE AEETVDDVL ESLQAVSLA 
INSRHPLHA HLETILQKV PTELRDRMN RRQLPEDTP 
TDLPSEKAL VRLHKQTIK SLQVFQRTE TQWNLLVEK 
IFDLEDTIK NQISRDKRF KRTFQKPRS LFRRFLYVP 
TVEWYWKCL FYGYTQKLL AVMAGLFSV AVVWSEVTF 
FNKQPVLSI FAAIVNRAK VKYDYFTIE LLSTIIILY 
LCYCAYSTV LKIKLLNLY YLAPHHQTN EYSLIFSGM 
MLSRLTPPL CLNFLGLIH MDSHIIKKQ VMETHYTQI 
MGHMDVISI ISDGFNVYF PMAILVFCL ATYFSVGSR 
LLSMLGFHQ FVGDDEMTT DLIEEGREL IKREKRKRQ 
RSEEASTRR TEFSERFPT TGRYRPRGS TDTIRPMTQ 
LENLDNTRS NLLQESHPL DSYESRRYT SEVDTRFPP 
DQDDIDARF GASTRINER YNSSQELEP RFADSYQSD 
GNYRNRVGA PPRGIFDDV
    CDS sequence
    • >TC012164 RA
      atgaattac gggccgctg atttcgaaa attttgctc 
tcttttatt cttgcctcg acggtcttg taccgatat 
gggaactgg ttcaggcac cacattgtg gtgaccttg 
attgtgctt cttgcttgg tatttcagt ttcttgatt 
attttcgcg cttccgttg gacgtaatt tcgacggtt 
taccgccag tgtaaggaa gaacatgac acttttgac 
aacgccact tcttcactg atgcaaaat gttacaaat 
aaaagtatt gaaaagtgt gaggagccg tggagtcga 
gtccctcat gaggttttt ccgaacttg tggcgtact 
gtgtattgg agcactcag tgcttaact tggctagtc 
atgcccatg atgcaatcg tacatcaaa gctggtgat 
tttacagtc aaaggcaaa ttgaaatcg gctgtaatc 
gacaatgcg atttattac ggctcttat ttgctaatc 
tgtggagtt ctactaatt tacttggcc ctccagcct 
gggattcac ctcgactgg tcaaaacta aaagcaatt 
gcttcgtca gctagcaat acttggggc ttattcctc 
ctagttttg ctgttcgga tacgcttta gttgaagtc 
cctcgaact ctgtggaac aactcaaac cacagtttt 
gtgctaaca cattcgtat ttcaaggcc gccaagctg 
agttcggac aagtgcgag gccgaggaa accgtcgac 
gatgtcctc gagtcgcta caagccgtc tcattggcc 
attaattcg cggcatcca ttgcacgcc cacttggag 
accattctg caaaaggtg cccacggaa ttacgggac 
agaatgaac cggaggcag ctccccgaa gacaccccc 
acggattta ccgagcgaa aaggcacta gtcaggctc 
cacaagcaa acgataaag tcgctccag gtgtttcag 
agaaccgaa acacagtgg aaccttctc gtcgagaag 
attttcgat cttgaggac actatcaaa aatcagatt 
tcgcgagat aagcgattt aaacgcaca ttccaaaaa 
ccccgatct cttttcagg aggtttttg tacgtcccg 
acagttgag tggtactgg aagtgtctt ttttatggc 
tacacgcaa aaattattg gctgttatg gctgggctt 
ttttcggtg gctgtggtt tggtccgaa gtgactttt 
ttcaacaaa cagccagtt ctctcgatt tttgccgct 
attgttaac agagctaaa gtcaagtac gactatttt 
acgatagag ttattgtcg accataata atcctgtac 
ttatgctac tgtgcttac tcaacggtc ctcaaaata 
aaactcctc aacttgtat tacttagca ccgcaccac 
caaacgaac gagtacagt ttaatcttc tcagggatg 
atgttgagc cgactgacg cctcctctg tgtttaaat 
ttcctcggc ttaattcac atggacagc cacataata 
aaaaaacaa gtcatggag acccactac acccaaatc 
atgggtcat atggacgtc atttcaata atctccgac 
ggcttcaac gtttacttc ccaatggcc attctagtc 
ttttgttta gcgacgtat ttcagcgtc ggctcgcgc 
cttttatca atgctgggt ttccaccag ttcgtcggc 
gacgacgaa atgaccacc gacctgatc gaagaaggc 
cgcgaattg atcaagcgg gaaaaacgc aaacgccag 
cggtcggag gaggccagc acgagacga accgaattc 
agtgaacgg ttcccgacc acggggagg tacaggccc 
cggggaagc accgacacg attcgtcca atgacgcaa 
ctggaaaat ttggacaac acgcgctca aatttgctg 
caagagtcg cacccgctg gactcgtac gagagtcgg 
aggtacacg agtgaggtc gatacgagg tttcccccg 
gatcaggac gatatcgat gcgcggttc ggcgccagc 
acgaggatt aacgaacgg tataacagt tcgcaggaa 
ctggagccg aggtttgcc gactcgtat cagtcggat 
ggtaactat agaaaccgc gtgggggcg cccccgagg 
ggcattttc gacgacgtt taa
    mRNA sequence
    • >TC012164 RA
      ttaataatt aagtcaaat gtgatgatt tgggcgaaa 
attggcgca aattattgg tgataaccc agtaatcaa 
tattggcca cattgccaa ctgacactt gtcaaagtg 
acgtttcgt gtgtgtgtg tgtgaaggt tcaatattt 
tcggaattt tcacgtgcg gattaattt tccggccac 
aggaaatct ctaattact tggacgatc gatattgat 
gactatgtt gtgatgtca tttagtgac aatgtccag 
tgtggaaaa accaaaacc gattttgcc taagaaagg 
acaaatcat cattgaaga catccagga cttggtgtt 
gtgcaaacc ccgtctgat cccccttaa gagtttcac 
tttggaaaa attggagaa aatgcaaca agaggccca 
aaaatcgca atcccagca gagtcccaa agacgagta 
aaaatgaat tacgggccg ctgatttcg aaaattttg 
ctctctttt attcttgcc tcgacggtc ttgtaccga 
tatgggaac tggttcagg caccacatt gtggtgacc 
ttgattgtg cttcttgct tggtatttc agtttcttg 
attattttc gcgcttccg ttggacgta atttcgacg 
gtttaccgc cagtgtaag gaagaacat gacactttt 
gacaacgcc acttcttca ctgatgcaa aatgttaca 
aataaaagt attgaaaag tgtgaggag ccgtggagt 
cgagtccct catgaggtt tttccgaac ttgtggcgt 
actgtgtat tggagcact cagtgctta acttggcta 
gtcatgccc atgatgcaa tcgtacatc aaagctggt 
gattttaca gtcaaaggc aaattgaaa tcggctgta 
atcgacaat gcgatttat tacggctct tatttgcta 
atctgtgga gttctacta atttacttg gccctccag 
cctgggatt cacctcgac tggtcaaaa ctaaaagca 
attgcttcg tcagctagc aatacttgg ggcttattc 
ctcctagtt ttgctgttc ggatacgct ttagttgaa 
gtccctcga actctgtgg aacaactca aaccacagt 
tttgtgcta acacattcg tatttcaag gccgccaag 
ctgagttcg gacaagtgc gaggccgag gaaaccgtc 
gacgatgtc ctcgagtcg ctacaagcc gtctcattg 
gccattaat tcgcggcat ccattgcac gcccacttg 
gagaccatt ctgcaaaag gtgcccacg gaattacgg 
gacagaatg aaccggagg cagctcccc gaagacacc 
cccacggat ttaccgagc gaaaaggca ctagtcagg 
ctccacaag caaacgata aagtcgctc caggtgttt 
cagagaacc gaaacacag tggaacctt ctcgtcgag 
aagattttc gatcttgag gacactatc aaaaatcag 
atttcgcga gataagcga tttaaacgc acattccaa 
aaaccccga tctcttttc aggaggttt ttgtacgtc 
ccgacagtt gagtggtac tggaagtgt cttttttat 
ggctacacg caaaaatta ttggctgtt atggctggg 
cttttttcg gtggctgtg gtttggtcc gaagtgact 
tttttcaac aaacagcca gttctctcg atttttgcc 
gctattgtt aacagagct aaagtcaag tacgactat 
tttacgata gagttattg tcgaccata ataatcctg 
tacttatgc tactgtgct tactcaacg gtcctcaaa 
ataaaactc ctcaacttg tattactta gcaccgcac 
caccaaacg aacgagtac agtttaatc ttctcaggg 
atgatgttg agccgactg acgcctcct ctgtgttta 
aatttcctc ggcttaatt cacatggac agccacata 
ataaaaaaa caagtcatg gagacccac tacacccaa 
atcatgggt catatggac gtcatttca ataatctcc 
gacggcttc aacgtttac ttcccaatg gccattcta 
gtcttttgt ttagcgacg tatttcagc gtcggctcg 
cgcctttta tcaatgctg ggtttccac cagttcgtc 
ggcgacgac gaaatgacc accgacctg atcgaagaa 
ggccgcgaa ttgatcaag cgggaaaaa cgcaaacgc 
cagcggtcg gaggaggcc agcacgaga cgaaccgaa 
ttcagtgaa cggttcccg accacgggg aggtacagg 
ccccgggga agcaccgac acgattcgt ccaatgacg 
caactggaa aatttggac aacacgcgc tcaaatttg 
ctgcaagag tcgcacccg ctggactcg tacgagagt 
cggaggtac acgagtgag gtcgatacg aggtttccc 
ccggatcag gacgatatc gatgcgcgg ttcggcgcc 
agcacgagg attaacgaa cggtataac agttcgcag 
gaactggag ccgaggttt gccgactcg tatcagtcg 
gatggtaac tatagaaac cgcgtgggg gcgcccccg 
aggggcatt ttcgacgac gtttaagtt gtttaagtc 
agtgatgtt tattatatt gcgttgaga aattatatt 
tttatattg tttataagg cagtttggt ttttcaggt 
ttcagctga aacccaccg tagtcaagt cttgtaata 
gtaggtttt agcgtccaa cgtttcacg gtagtaact 
tatttcagt gtaatcacg atttcttct gggtcggat 
ttttaaatc aagttggca acactaaat aatgagtcg 
caggccact ttgatgaga aacttaatt gaaagcctg 
gcatttgtt atgtaatat tccaattac acgtgtaat 
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