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Gene information
General information
TC012164 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4004903..4008500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012164
    iBeetle screen iB_07601

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012164 RA
      MNYGPLISK ILLSFILAS TVLYRYGNW FRHHIVVTL 
      IVLLAWYFS FLIIFALPL DVISTVYRQ CKEEHDTFD 
      NATSSLMQN VTNKSIEKC EEPWSRVPH EVFPNLWRT 
      VYWSTQCLT WLVMPMMQS YIKAGDFTV KGKLKSAVI 
      DNAIYYGSY LLICGVLLI YLALQPGIH LDWSKLKAI 
      ASSASNTWG LFLLVLLFG YALVEVPRT LWNNSNHSF 
      VLTHSYFKA AKLSSDKCE AEETVDDVL ESLQAVSLA 
      INSRHPLHA HLETILQKV PTELRDRMN RRQLPEDTP 
      TDLPSEKAL VRLHKQTIK SLQVFQRTE TQWNLLVEK 
      IFDLEDTIK NQISRDKRF KRTFQKPRS LFRRFLYVP 
      TVEWYWKCL FYGYTQKLL AVMAGLFSV AVVWSEVTF 
      FNKQPVLSI FAAIVNRAK VKYDYFTIE LLSTIIILY 
      LCYCAYSTV LKIKLLNLY YLAPHHQTN EYSLIFSGM 
      MLSRLTPPL CLNFLGLIH MDSHIIKKQ VMETHYTQI 
      MGHMDVISI ISDGFNVYF PMAILVFCL ATYFSVGSR 
      LLSMLGFHQ FVGDDEMTT DLIEEGREL IKREKRKRQ 
      RSEEASTRR TEFSERFPT TGRYRPRGS TDTIRPMTQ 
      LENLDNTRS NLLQESHPL DSYESRRYT SEVDTRFPP 
      DQDDIDARF GASTRINER YNSSQELEP RFADSYQSD 
      GNYRNRVGA PPRGIFDDV
    CDS sequence
    • >TC012164 RA
      atgaattac gggccgctg atttcgaaa attttgctc 
      tcttttatt cttgcctcg acggtcttg taccgatat 
      gggaactgg ttcaggcac cacattgtg gtgaccttg 
      attgtgctt cttgcttgg tatttcagt ttcttgatt 
      attttcgcg cttccgttg gacgtaatt tcgacggtt 
      taccgccag tgtaaggaa gaacatgac acttttgac 
      aacgccact tcttcactg atgcaaaat gttacaaat 
      aaaagtatt gaaaagtgt gaggagccg tggagtcga 
      gtccctcat gaggttttt ccgaacttg tggcgtact 
      gtgtattgg agcactcag tgcttaact tggctagtc 
      atgcccatg atgcaatcg tacatcaaa gctggtgat 
      tttacagtc aaaggcaaa ttgaaatcg gctgtaatc 
      gacaatgcg atttattac ggctcttat ttgctaatc 
      tgtggagtt ctactaatt tacttggcc ctccagcct 
      gggattcac ctcgactgg tcaaaacta aaagcaatt 
      gcttcgtca gctagcaat acttggggc ttattcctc 
      ctagttttg ctgttcgga tacgcttta gttgaagtc 
      cctcgaact ctgtggaac aactcaaac cacagtttt 
      gtgctaaca cattcgtat ttcaaggcc gccaagctg 
      agttcggac aagtgcgag gccgaggaa accgtcgac 
      gatgtcctc gagtcgcta caagccgtc tcattggcc 
      attaattcg cggcatcca ttgcacgcc cacttggag 
      accattctg caaaaggtg cccacggaa ttacgggac 
      agaatgaac cggaggcag ctccccgaa gacaccccc 
      acggattta ccgagcgaa aaggcacta gtcaggctc 
      cacaagcaa acgataaag tcgctccag gtgtttcag 
      agaaccgaa acacagtgg aaccttctc gtcgagaag 
      attttcgat cttgaggac actatcaaa aatcagatt 
      tcgcgagat aagcgattt aaacgcaca ttccaaaaa 
      ccccgatct cttttcagg aggtttttg tacgtcccg 
      acagttgag tggtactgg aagtgtctt ttttatggc 
      tacacgcaa aaattattg gctgttatg gctgggctt 
      ttttcggtg gctgtggtt tggtccgaa gtgactttt 
      ttcaacaaa cagccagtt ctctcgatt tttgccgct 
      attgttaac agagctaaa gtcaagtac gactatttt 
      acgatagag ttattgtcg accataata atcctgtac 
      ttatgctac tgtgcttac tcaacggtc ctcaaaata 
      aaactcctc aacttgtat tacttagca ccgcaccac 
      caaacgaac gagtacagt ttaatcttc tcagggatg 
      atgttgagc cgactgacg cctcctctg tgtttaaat 
      ttcctcggc ttaattcac atggacagc cacataata 
      aaaaaacaa gtcatggag acccactac acccaaatc 
      atgggtcat atggacgtc atttcaata atctccgac 
      ggcttcaac gtttacttc ccaatggcc attctagtc 
      ttttgttta gcgacgtat ttcagcgtc ggctcgcgc 
      cttttatca atgctgggt ttccaccag ttcgtcggc 
      gacgacgaa atgaccacc gacctgatc gaagaaggc 
      cgcgaattg atcaagcgg gaaaaacgc aaacgccag 
      cggtcggag gaggccagc acgagacga accgaattc 
      agtgaacgg ttcccgacc acggggagg tacaggccc 
      cggggaagc accgacacg attcgtcca atgacgcaa 
      ctggaaaat ttggacaac acgcgctca aatttgctg 
      caagagtcg cacccgctg gactcgtac gagagtcgg 
      aggtacacg agtgaggtc gatacgagg tttcccccg 
      gatcaggac gatatcgat gcgcggttc ggcgccagc 
      acgaggatt aacgaacgg tataacagt tcgcaggaa 
      ctggagccg aggtttgcc gactcgtat cagtcggat 
      ggtaactat agaaaccgc gtgggggcg cccccgagg 
      ggcattttc gacgacgtt taa
    mRNA sequence
    • >TC012164 RA
      ttaataatt aagtcaaat gtgatgatt tgggcgaaa 
      attggcgca aattattgg tgataaccc agtaatcaa 
      tattggcca cattgccaa ctgacactt gtcaaagtg 
      acgtttcgt gtgtgtgtg tgtgaaggt tcaatattt 
      tcggaattt tcacgtgcg gattaattt tccggccac 
      aggaaatct ctaattact tggacgatc gatattgat 
      gactatgtt gtgatgtca tttagtgac aatgtccag 
      tgtggaaaa accaaaacc gattttgcc taagaaagg 
      acaaatcat cattgaaga catccagga cttggtgtt 
      gtgcaaacc ccgtctgat cccccttaa gagtttcac 
      tttggaaaa attggagaa aatgcaaca agaggccca 
      aaaatcgca atcccagca gagtcccaa agacgagta 
      aaaatgaat tacgggccg ctgatttcg aaaattttg 
      ctctctttt attcttgcc tcgacggtc ttgtaccga 
      tatgggaac tggttcagg caccacatt gtggtgacc 
      ttgattgtg cttcttgct tggtatttc agtttcttg 
      attattttc gcgcttccg ttggacgta atttcgacg 
      gtttaccgc cagtgtaag gaagaacat gacactttt 
      gacaacgcc acttcttca ctgatgcaa aatgttaca 
      aataaaagt attgaaaag tgtgaggag ccgtggagt 
      cgagtccct catgaggtt tttccgaac ttgtggcgt 
      actgtgtat tggagcact cagtgctta acttggcta 
      gtcatgccc atgatgcaa tcgtacatc aaagctggt 
      gattttaca gtcaaaggc aaattgaaa tcggctgta 
      atcgacaat gcgatttat tacggctct tatttgcta 
      atctgtgga gttctacta atttacttg gccctccag 
      cctgggatt cacctcgac tggtcaaaa ctaaaagca 
      attgcttcg tcagctagc aatacttgg ggcttattc 
      ctcctagtt ttgctgttc ggatacgct ttagttgaa 
      gtccctcga actctgtgg aacaactca aaccacagt 
      tttgtgcta acacattcg tatttcaag gccgccaag 
      ctgagttcg gacaagtgc gaggccgag gaaaccgtc 
      gacgatgtc ctcgagtcg ctacaagcc gtctcattg 
      gccattaat tcgcggcat ccattgcac gcccacttg 
      gagaccatt ctgcaaaag gtgcccacg gaattacgg 
      gacagaatg aaccggagg cagctcccc gaagacacc 
      cccacggat ttaccgagc gaaaaggca ctagtcagg 
      ctccacaag caaacgata aagtcgctc caggtgttt 
      cagagaacc gaaacacag tggaacctt ctcgtcgag 
      aagattttc gatcttgag gacactatc aaaaatcag 
      atttcgcga gataagcga tttaaacgc acattccaa 
      aaaccccga tctcttttc aggaggttt ttgtacgtc 
      ccgacagtt gagtggtac tggaagtgt cttttttat 
      ggctacacg caaaaatta ttggctgtt atggctggg 
      cttttttcg gtggctgtg gtttggtcc gaagtgact 
      tttttcaac aaacagcca gttctctcg atttttgcc 
      gctattgtt aacagagct aaagtcaag tacgactat 
      tttacgata gagttattg tcgaccata ataatcctg 
      tacttatgc tactgtgct tactcaacg gtcctcaaa 
      ataaaactc ctcaacttg tattactta gcaccgcac 
      caccaaacg aacgagtac agtttaatc ttctcaggg 
      atgatgttg agccgactg acgcctcct ctgtgttta 
      aatttcctc ggcttaatt cacatggac agccacata 
      ataaaaaaa caagtcatg gagacccac tacacccaa 
      atcatgggt catatggac gtcatttca ataatctcc 
      gacggcttc aacgtttac ttcccaatg gccattcta 
      gtcttttgt ttagcgacg tatttcagc gtcggctcg 
      cgcctttta tcaatgctg ggtttccac cagttcgtc 
      ggcgacgac gaaatgacc accgacctg atcgaagaa 
      ggccgcgaa ttgatcaag cgggaaaaa cgcaaacgc 
      cagcggtcg gaggaggcc agcacgaga cgaaccgaa 
      ttcagtgaa cggttcccg accacgggg aggtacagg 
      ccccgggga agcaccgac acgattcgt ccaatgacg 
      caactggaa aatttggac aacacgcgc tcaaatttg 
      ctgcaagag tcgcacccg ctggactcg tacgagagt 
      cggaggtac acgagtgag gtcgatacg aggtttccc 
      ccggatcag gacgatatc gatgcgcgg ttcggcgcc 
      agcacgagg attaacgaa cggtataac agttcgcag 
      gaactggag ccgaggttt gccgactcg tatcagtcg 
      gatggtaac tatagaaac cgcgtgggg gcgcccccg 
      aggggcatt ttcgacgac gtttaagtt gtttaagtc 
      agtgatgtt tattatatt gcgttgaga aattatatt 
      tttatattg tttataagg cagtttggt ttttcaggt 
      ttcagctga aacccaccg tagtcaagt cttgtaata 
      gtaggtttt agcgtccaa cgtttcacg gtagtaact 
      tatttcagt gtaatcacg atttcttct gggtcggat 
      ttttaaatc aagttggca acactaaat aatgagtcg 
      caggccact ttgatgaga aacttaatt gaaagcctg 
      gcatttgtt atgtaatat tccaattac acgtgtaat 
      aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY