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Gene information
General information
TC012304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3958562..3961534
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012304
    iBeetle screen iB_07599

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012304 RA
      MEVVANNYL PGDQRLVDK IVYELKAQG IFDQFRKEC 
      FADVDTKPA YQNLRQRVE GSVTGFLKE QSWRPDMNK 
      NQVREMLRK RITESGYLE TGVERIVDQ VVNPKINTV 
      FLPQVEEVV YSYLGIKKP SKSEKSEQC DDLLPTDLE 
      AVSPESEPV DKKDDKSTD TFQDLDETK TEEDESPPF 
      EPLEEQTTY VPQEENSVD SHMSGFSGL ASHESNQSN 
      ENKITPMEQ DSQMSRNSS DSRLSIITS DDNSKMDIC 
      EDSQSKSIK STDSNHKHD DKKHKSSER HKSRSESKS 
      DKDRKSSKD SRSRHSSSS KDKDKSRDR RDSKSSSKP 
      KENDKDKSK DKDRNRSSS KHTSSKDKD KNAKKDSRS 
      DKDKPRSSS SHKSRDKSS DSKSKEKSS SDRHKHSSS 
      HSDRHKKDK DKKKDSKLK DDHYSSKDK RNDRRSTDR 
      DSNDGQSSR PPTNPSDNS NSQSQKSQD SNSGGGDSG 
      NSDHTESTT KAPVVITGA VENSEVMIS PPETQRVKV 
      LKPKCASNI QEALKLMKI RKQLAKLEK QNQLSLASV 
      DVTSPIVLS SSINLDDLN QNCLKKEHE NKIEDVVPH 
      LQNTVISQD SWDTMEAKL REAVCQEMD ESGDEDEKC 
      RFFESVDIK KIEFLQFVE LFISKFEKY QRLRKRNGG 
      KRKIEDNDL KNNNKQLKK QKVGKSKTT NKNNNHAVA 
      NEKFPLPLS PAESDKSTG KKEEISTPK AKRNNVNKV 
      NNQRYSSDD LYKPRPTFI STRRRGAKN LEN
    CDS sequence
    • >TC012304 RA
      atggaggtt gttgccaat aattatcta ccgggtgat 
      cagcgttta gtcgataag atcgtctac gagctgaag 
      gcccagggg attttcgat caattccgc aaggagtgt 
      ttcgccgat gtcgacacc aagccagcc taccaaaac 
      ctccgccaa agagtagaa ggcagcgtc acaggtttc 
      ctcaaagag cagtcctgg aggccggac atgaacaag 
      aaccaagtg cgggaaatg ttacgtaag aggatcacc 
      gagtctggg tatctggaa acgggggtt gagcgcatt 
      gtcgaccaa gttgtcaac ccgaagatc aatacggtg 
      tttttgccc caggtggaa gaggttgtt tacagttac 
      ttgggcatc aagaaaccc agcaaaagc gaaaagagt 
      gaacagtgt gacgatctg ttgcctact gatctcgag 
      gcggtgtcc cctgagtcc gagcccgtt gacaagaag 
      gacgataaa agtacggac acgtttcaa gatttggac 
      gaaacgaag actgaggag gacgagtcg cccccgttt 
      gagcccctg gaggaacag accacctat gtgccccag 
      gaggagaat tccgttgat tcgcacatg tcagggttt 
      tcaggttta gctagtcat gagtcaaat caaagtaat 
      gaaaataaa ataacaccg atggagcaa gattcgcaa 
      atgtcgcgc aacagctct gatagccgt ttatcaatc 
      ataacgtca gacgataac tcaaaaatg gatatttgc 
      gaagactca cagtcaaaa tccatcaaa tcaactgac 
      tcaaaccac aaacatgac gataaaaaa cacaaatcg 
      tccgagcgt cacaaatcg cgcagcgag agcaagtct 
      gataaagac aggaagagt agcaaagat tcaagatct 
      cggcacagt tcgtcttcg aaagataaa gacaagagc 
      cgcgatcgt cgcgattcg aagagctca agtaagccc 
      aaagagaac gacaaagac aagtccaaa gataaggat 
      cgcaatagg agctccagc aagcacacc agtagcaaa 
      gacaaggat aaaaacgcg aaaaaagac tctcgatct 
      gataaagat aagcctcgg tcaagcagc agccacaag 
      tcccgtgat aaatccagc gattccaaa tctaaagaa 
      aagtcttcc tccgatcgc cacaaacac tcgtcctcg 
      cacagcgac cgccacaag aaggataag gacaagaaa 
      aaggattcg aaattgaaa gacgatcat tacagttcg 
      aaagacaag agaaatgac cgccgatcg accgatcgc 
      gattcgaac gacggccag tcaagtcgc ccgcctacc 
      aatccctcc gataactcc aattcccag tcacagaag 
      agccaagac tcgaattca gggggcggt gattcgggg 
      aattccgat cacaccgaa agcacgacc aaagccccc 
      gtggtgatc acaggggct gtggaaaat tccgaagtg 
      atgatttcg cccccggag actcagcga gtcaaggtt 
      ttgaaaccg aaatgtgcc tcgaatatt caagaggct 
      ttgaagttg atgaaaatt cgcaaacag ttagccaag 
      ttggagaag cagaatcag ttgagtttg gcgagtgtt 
      gatgtgact tcgcccatt gtcctctcg tcgagtatc 
      aatctggac gacttgaac cagaattgt cttaaaaag 
      gagcatgaa aataagatc gaggacgtg gtgccgcac 
      ttacagaac actgtaatt agtcaagac agttgggac 
      accatggag gcgaaactg agggaggca gtgtgccag 
      gagatggat gaaagtgga gatgaggac gagaaatgt 
      agatttttc gaaagtgtg gacattaag aaaatcgag 
      tttttgcaa tttgtcgag ctgtttatc agtaaattt 
      gagaagtat caaaggttg cggaagaga aatgggggg 
      aaacgaaaa attgaagac aacgatttg aagaataat 
      aacaagcag ttgaagaag caaaaggtt gggaagagt 
      aagacaacg aacaagaat aataatcat gccgttgcg 
      aatgagaaa tttccactg ccgttgagt ccggctgaa 
      agtgacaag tcaacaggt aagaaggaa gaaatttcg 
      acaccgaag gccaaacgt aacaacgtt aacaaagtc 
      aataatcag agatactcc agcgacgac ctgtacaaa 
      cctagacca actttcatt agcactaga cgtagaggt 
      gccaaaaat ctggagaac tga
    mRNA sequence
    • >TC012304 RA
      gaataagag ttacaaatc gaaactccc caactgtca 
      tgtgagtga aaaaacaaa aacaaatag tgctgcaat 
      tatttatta tggaggttg ttgccaata attatctac 
      cgggtgatc agcgtttag tcgataaga tcgtctacg 
      agctgaagg cccagggga ttttcgatc aattccgca 
      aggagtgtt tcgccgatg tcgacacca agccagcct 
      accaaaacc tccgccaaa gagtagaag gcagcgtca 
      caggtttcc tcaaagagc agtcctgga ggccggaca 
      tgaacaaga accaagtgc gggaaatgt tacgtaaga 
      ggatcaccg agtctgggt atctggaaa cgggggttg 
      agcgcattg tcgaccaag ttgtcaacc cgaagatca 
      atacggtgt ttttgcccc aggtggaag aggttgttt 
      acagttact tgggcatca agaaaccca gcaaaagcg 
      aaaagagtg aacagtgtg acgatctgt tgcctactg 
      atctcgagg cggtgtccc ctgagtccg agcccgttg 
      acaagaagg acgataaaa gtacggaca cgtttcaag 
      atttggacg aaacgaaga ctgaggagg acgagtcgc 
      ccccgtttg agcccctgg aggaacaga ccacctatg 
      tgccccagg aggagaatt ccgttgatt cgcacatgt 
      cagggtttt caggtttag ctagtcatg agtcaaatc 
      aaagtaatg aaaataaaa taacaccga tggagcaag 
      attcgcaaa tgtcgcgca acagctctg atagccgtt 
      tatcaatca taacgtcag acgataact caaaaatgg 
      atatttgcg aagactcac agtcaaaat ccatcaaat 
      caactgact caaaccaca aacatgacg ataaaaaac 
      acaaatcgt ccgagcgtc acaaatcgc gcagcgaga 
      gcaagtctg ataaagaca ggaagagta gcaaagatt 
      caagatctc ggcacagtt cgtcttcga aagataaag 
      acaagagcc gcgatcgtc gcgattcga agagctcaa 
      gtaagccca aagagaacg acaaagaca agtccaaag 
      ataaggatc gcaatagga gctccagca agcacacca 
      gtagcaaag acaaggata aaaacgcga aaaaagact 
      ctcgatctg ataaagata agcctcggt caagcagca 
      gccacaagt cccgtgata aatccagcg attccaaat 
      ctaaagaaa agtcttcct ccgatcgcc acaaacact 
      cgtcctcgc acagcgacc gccacaaga aggataagg 
      acaagaaaa aggattcga aattgaaag acgatcatt 
      acagttcga aagacaaga gaaatgacc gccgatcga 
      ccgatcgcg attcgaacg acggccagt caagtcgcc 
      cgcctacca atccctccg ataactcca attcccagt 
      cacagaaga gccaagact cgaattcag ggggcggtg 
      attcgggga attccgatc acaccgaaa gcacgacca 
      aagcccccg tggtgatca caggggctg tggaaaatt 
      ccgaagtga tgatttcgc ccccggaga ctcagcgag 
      tcaaggttt tgaaaccga aatgtgcct cgaatattc 
      aagaggctt tgaagttga tgaaaattc gcaaacagt 
      tagccaagt tggagaagc agaatcagt tgagtttgg 
      cgagtgttg atgtgactt cgcccattg tcctctcgt 
      cgagtatca atctggacg acttgaacc agaattgtc 
      ttaaaaagg agcatgaaa ataagatcg aggacgtgg 
      tgccgcact tacagaaca ctgtaatta gtcaagaca 
      gttgggaca ccatggagg cgaaactga gggaggcag 
      tgtgccagg agatggatg aaagtggag atgaggacg 
      agaaatgta gatttttcg aaagtgtgg acattaaga 
      aaatcgagt ttttgcaat ttgtcgagc tgtttatca 
      gtaaatttg agaagtatc aaaggttgc ggaagagaa 
      atgggggga aacgaaaaa ttgaagaca acgatttga 
      agaataata acaagcagt tgaagaagc aaaaggttg 
      ggaagagta agacaacga acaagaata ataatcatg 
      ccgttgcga atgagaaat ttccactgc cgttgagtc 
      cggctgaaa gtgacaagt caacaggta agaaggaag 
      aaatttcga caccgaagg ccaaacgta acaacgtta 
      acaaagtca ataatcaga gatactcca gcgacgacc 
      tgtacaaac ctagaccaa ctttcatta gcactagac 
      gtagaggtg ccaaaaatc tggagaact gactaaggt 
      tttattttt ctcgtagaa taggaatta gaagtaaat 
      aaatatttt tatatgtt
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