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Gene information
General information
TC001743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8132723..8135355
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001743
    iBeetle screen iB_07586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001743 RA
      MRLHQFCLL LVVTTVSTS PTTPASSVK CIDGCECRE 
      QKPILRTTF EIFNCKTPF VLNKSALSN VPKDRAFIK 
      FKSTDVQHI TSDAFEDFT QLEEVDFDN SRVGTIDSE 
      AFNRKFLGA ISFKNVHFA EIPNLKADF LEEFICEDC 
      HLSKVPNLD NLPSLTFIN FANNRITSI HESALSKLK 
      KLEEVNLSN NSISELPMN LFVQNEIDT LKLDYNPLK 
      SFTFHDDNV LESLSLAHC NLTVFDENS TKNLTFLTS 
      LDLSGNNIV VLPLDTFNP MKSLETIDL SDNHLVELD 
      DNIFSENSR LDTINLNNN NLKKLPNFQ TKAKLFQTS 
      TFSCKNCGL KSATGLANM ARLTKIDLS NNEITDIEG 
      AFSEMPILK KLFLSNNHI ASIGLKSPE LETLDLSNN 
      PLLPLDPVT FTALPSLKI LNVSSCALE KIWSNYNAE 
      LPKLTQLYL GQNQLSVIT LDDVKIMPR LKVIDLREN 
      PWKCTDDLE EVVKYFVKE SVYPADIGT IQFKTHEEL 
      TFAENTKLV LQPVWPKIL PNCDFDFGE EEGTEPSVH 
      IVENEQDED DDFYDEDEY EENDEDEEI PVEKAMEDP 
      SPFNLARAT YILSITSVF ILTAVLVSV VAIVLTLLI 
      LKRNKSFNM HTGNLPRLK IPLWHTTPG QKKHSGSVY 
      RPLSEDLYG SRTPIINRY EFKQTPQVH NSLP
    CDS sequence
    • >TC001743 RA
      atgcgattg caccaattt tgtctactt cttgtcgtt 
      acgaccgta agcacaagt cctacgact cctgcaagc 
      agcgtcaag tgcattgat ggttgtgaa tgcagggag 
      cagaaacca atattgcga acaaccttc gaaattttt 
      aattgcaaa acccccttt gtgctcaac aagagcgcg 
      cttagcaat gttcccaaa gatcgcgcg tttatcaaa 
      tttaaaagt accgacgtc caacacatt actagtgat 
      gctttcgaa gacttcaca caattagag gaagtggat 
      ttcgacaac tcccgcgtt ggcacgatc gatagtgaa 
      gcattcaat cggaaattt ctaggtgct atcagtttt 
      aaaaacgtc cattttgcc gaaatacct aatttgaaa 
      gctgacttt ctcgaagaa ttcatctgt gaggattgt 
      cacttgtcc aaagtaccc aacttagat aatctaccc 
      tcactcaca tttataaat ttcgcgaac aatcgtatc 
      acaagtatt catgaatcg gcgctttca aaactgaaa 
      aagttggaa gaagttaat ctgtcgaac aacagcatt 
      tccgaattg cctatgaac ctgttcgtt caaaacgaa 
      atcgataca ctaaaactg gactacaac cccttgaaa 
      agcttcact tttcatgac gacaacgtc ctggaaagt 
      ctaagtctt gcccattgc aacctaaca gtctttgat 
      gaaaattcc accaaaaat ttaaccttc ctgactagt 
      ctcgacttg agcggtaac aacattgtt gttttgcca 
      cttgacacg ttcaatccg atgaaatcg ctcgagacc 
      atcgatcta tcagacaac cacttggtc gaactggac 
      gataatatc ttttcagaa aacagtcga ctcgacact 
      atcaacctc aataacaac aatcttaaa aaactgccc 
      aatttccag acaaaggct aaactgttc caaacctca 
      accttctcg tgcaaaaac tgcggcttg aaatcggcc 
      acaggtctc gccaacatg gcaagactc actaaaatc 
      gatctgtcc aacaacgaa atcacagac atcgaaggc 
      gcgttcagt gaaatgccc attctcaaa aaactcttc 
      ttgtccaac aaccacatt gcctcaatc gggcttaaa 
      tcacccgag ttggagaca ctcgatttg tccaataac 
      ccgctcttg ccactggac cccgttacg ttcacagcg 
      cttccttcg ttgaagatt ttgaatgtt agcagttgc 
      gctcttgaa aaaatttgg agcaattac aacgccgaa 
      ctcccgaaa ctgacccaa ctctacctg ggccagaat 
      caattgtct gtgatcaca cttgacgat gtaaagatc 
      atgccaaga ctgaaagtg attgatttg cgggagaat 
      ccatggaag tgtacggac gaccttgag gaagttgtt 
      aagtacttt gtgaaagag tccgtttat cctgcggat 
      atcggaact atccaattt aagacgcat gaagaattg 
      acgtttgcc gaaaacacg aaactcgtt cttcaacca 
      gtttggccg aaaatcctc ccgaattgc gattttgac 
      ttcggggag gaagaaggt accgaacct tcggtacat 
      atcgtagaa aatgagcaa gacgaagac gatgatttt 
      tacgatgag gacgaatat gaggaaaat gatgaggat 
      gaggaaatt ccggtcgag aaggcaatg gaagaccct 
      tcacccttc aacctagca agggccact tacatcctt 
      tccatcact tcggtcttt attctgact gctgtgtta 
      gtatccgtg gtcgcgatt gttctcact cttttgatt 
      ttgaagagg aataagtcg ttcaatatg cacacgggc 
      aatcttcca cgtttgaag atacccttg tggcataca 
      acccccggg caaaagaaa cacagtggt tccgtgtac 
      aggccactg tcggaagac ctttacgga tcgcgtact 
      cccatcatc aatcgatac gagtttaaa cagacgccc 
      caagttcat aactcccta ccttaa
    mRNA sequence
    • >TC001743 RA
      ggagggggt attacctgt agtactcgc aggcaggta 
      cctacagaa aggtggaaa ttacattgt gattcattg 
      ctagtgcgg aaagtgttt gttccgcgg actcctcta 
      cgttaattc aatcccata agtgtgaaa aaccaatga 
      tgcgattgc accaatttt gtctacttc ttgtcgtta 
      cgaccgtaa gcacaagtc ctacgactc ctgcaagca 
      gcgtcaagt gcattgatg gttgtgaat gcagggagc 
      agaaaccaa tattgcgaa caaccttcg aaattttta 
      attgcaaaa ccccctttg tgctcaaca agagcgcgc 
      ttagcaatg ttcccaaag atcgcgcgt ttatcaaat 
      ttaaaagta ccgacgtcc aacacatta ctagtgatg 
      ctttcgaag acttcacac aattagagg aagtggatt 
      tcgacaact cccgcgttg gcacgatcg atagtgaag 
      cattcaatc ggaaatttc taggtgcta tcagtttta 
      aaaacgtcc attttgccg aaataccta atttgaaag 
      ctgactttc tcgaagaat tcatctgtg aggattgtc 
      acttgtcca aagtaccca acttagata atctaccct 
      cactcacat ttataaatt tcgcgaaca atcgtatca 
      caagtattc atgaatcgg cgctttcaa aactgaaaa 
      agttggaag aagttaatc tgtcgaaca acagcattt 
      ccgaattgc ctatgaacc tgttcgttc aaaacgaaa 
      tcgatacac taaaactgg actacaacc ccttgaaaa 
      gcttcactt ttcatgacg acaacgtcc tggaaagtc 
      taagtcttg cccattgca acctaacag tctttgatg 
      aaaattcca ccaaaaatt taaccttcc tgactagtc 
      tcgacttga gcggtaaca acattgttg ttttgccac 
      ttgacacgt tcaatccga tgaaatcgc tcgagacca 
      tcgatctat cagacaacc acttggtcg aactggacg 
      ataatatct tttcagaaa acagtcgac tcgacacta 
      tcaacctca ataacaaca atcttaaaa aactgccca 
      atttccaga caaaggcta aactgttcc aaacctcaa 
      ccttctcgt gcaaaaact gcggcttga aatcggcca 
      caggtctcg ccaacatgg caagactca ctaaaatcg 
      atctgtcca acaacgaaa tcacagaca tcgaaggcg 
      cgttcagtg aaatgccca ttctcaaaa aactcttct 
      tgtccaaca accacattg cctcaatcg ggcttaaat 
      cacccgagt tggagacac tcgatttgt ccaataacc 
      cgctcttgc cactggacc ccgttacgt tcacagcgc 
      ttccttcgt tgaagattt tgaatgtta gcagttgcg 
      ctcttgaaa aaatttgga gcaattaca acgccgaac 
      tcccgaaac tgacccaac tctacctgg gccagaatc 
      aattgtctg tgatcacac ttgacgatg taaagatca 
      tgccaagac tgaaagtga ttgatttgc gggagaatc 
      catggaagt gtacggacg accttgagg aagttgtta 
      agtactttg tgaaagagt ccgtttatc ctgcggata 
      tcggaacta tccaattta agacgcatg aagaattga 
      cgtttgccg aaaacacga aactcgttc ttcaaccag 
      tttggccga aaatcctcc cgaattgcg attttgact 
      tcggggagg aagaaggta ccgaacctt cggtacata 
      tcgtagaaa atgagcaag acgaagacg atgattttt 
      acgatgagg acgaatatg aggaaaatg atgaggatg 
      aggaaattc cggtcgaga aggcaatgg aagaccctt 
      cacccttca acctagcaa gggccactt acatccttt 
      ccatcactt cggtcttta ttctgactg ctgtgttag 
      tatccgtgg tcgcgattg ttctcactc ttttgattt 
      tgaagagga ataagtcgt tcaatatgc acacgggca 
      atcttccac gtttgaaga tacccttgt ggcatacaa 
      cccccgggc aaaagaaac acagtggtt ccgtgtaca 
      ggccactgt cggaagacc tttacggat cgcgtactc 
      ccatcatca atcgatacg agtttaaac agacgcccc 
      aagttcata actccctac cttaattat ttacttaac 
      tgtgtttta tccataagt aacgttaga tattgttta 
      taatttgtt ttatgtttt gtttaagtg ataatacgt 
      aggttcgta tctatgcca tcactatat taattatac 
      tgagacaca tgtaaaaaa aaacatttg ataataaat 
      tatttaact gggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY