Status
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Gene information
General information
TC006757 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13796899..13798793
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006757
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07578

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006757 RA
      MLRGCFEPP TLVNAQMGE FQDTPKTEQ PPVFENVQI 
      KTETEHNSS PEGESDEDF ELKLVKSED FERNNDALD 
      NETTRYKCN ICSLAFKVL ADYRTHRKQ HFVEKRTCK 
      VCHTVCQSI SKLEIHMNT HLGLKPYKC TVCAKNFVS 
      SNHLKLHGR CHSDEKRYS CQKCSKAFR YLGSLRSHM 
      VTHNDIKEF ECKHCGANS RNVEAHRKH LQSHGQKIE 
      IKCELCNRN FATERLLQT HLNSHSELQ YPCEYCGRI 
      YPSLYRLKR HIKRAHIPN VCDECNAVF YDRALFTRH 
      KKQHNEGKP VSCSVCGKN FDKQKNLSE HVRLQHKED 
      GNVHKCDLC EKIFINASL LRNHIKTHD KCFKCPFCA 
      KLFSSRYNL DTHLVTHTG ERNHKCDIC GNVYSTKTS 
      LKNHKATHS DARNFKCDQ CPKLFKTNR RLYVHKFSH 
      ATEEKFECG VCNARFKVK QYLKYHMIK HSSNKPFEC 
      KVCQKKFKH KKSWEKHSS HDKHLRNVK EEAQNCGVC 
      GHVCVTKTD LLEHSRLCH EHGIKQESC
    CDS sequence
    • >TC006757 RA
      atgttgagg gggtgtttt gagccccca acgctcgta 
      aacgcgcaa atgggcgag ttccaagac acccccaag 
      acagaacaa cccccagtt tttgaaaac gttcaaatt 
      aagaccgaa accgagcac aattctagt cctgagggc 
      gagtccgac gaggatttc gagctgaag ctcgttaaa 
      agtgaagat ttcgagcgc aacaacgat gcactggat 
      aacgaaacc acacggtat aagtgcaac atctgttcg 
      ctggctttc aaagttttg gcggactat cgaacgcac 
      cggaagcaa cattttgtg gaaaaaagg acatgtaag 
      gtgtgccat acagtgtgc caaagcatc agtaaactc 
      gagatacat atgaacacg catttgggg ctcaagccg 
      tacaagtgc acagtctgc gccaaaaat ttcgtctca 
      agtaatcat ctaaaactg cacgggagg tgccattcg 
      gatgagaaa cgatactct tgccagaaa tgcagcaag 
      gcgtttcgg tacttgggg tccctgagg agtcacatg 
      gtcacacac aacgacatc aaggagttt gagtgtaag 
      cactgtggc gccaactct cgcaacgtt gaagcgcac 
      cgtaaacac ctccaatcc cacggccaa aaaatcgaa 
      atcaagtgc gagctctgc aaccggaac tttgcaacc 
      gaacgccta ctgcaaacc cacctaaac tcgcactct 
      gagttgcaa tacccttgc gaatactgc ggccgcatc 
      tacccctcg ttgtatcgc ctcaaacgc cacataaaa 
      cgcgcacac attcccaac gtttgcgac gaatgcaac 
      gctgttttc tacgataga gctctattc acacgccac 
      aagaaacaa cacaacgag ggcaagccc gtatcgtgt 
      tcagtttgt ggcaaaaac ttcgacaaa cagaagaac 
      ttgagcgaa catgtgcgc ctccaacac aaggaggac 
      ggtaatgtc cataaatgt gacttgtgc gagaaaatt 
      tttatcaac gcatcgctg cttcgcaac catatcaag 
      acgcatgat aagtgcttt aaatgcccg ttttgcgct 
      aaattgttc agttcgagg tataatctc gacacgcat 
      ctagtgaca cacactggg gagaggaac cacaagtgc 
      gacatctgt gggaatgtg tactcgacg aagaccagc 
      cttaagaac cacaaggcg actcatagt gatgccaga 
      aatttcaag tgtgaccaa tgccccaaa ttattcaaa 
      actaatcgg agattatac gttcataaa ttttcgcat 
      gccactgaa gaaaagttc gagtgtggg gtttgtaat 
      gcgaggttt aaagtcaaa cagtacttg aaatatcac 
      atgatcaag cattcgagc aataagccg ttcgaatgc 
      aaagtctgt caaaaaaaa ttcaagcac aagaagtcg 
      tgggagaaa cattcgagt cacgacaaa cacttgcga 
      aacgttaaa gaggaggcg caaaattgt ggcgtttgt 
      gggcacgtg tgtgtcact aaaaccgac ctgctggaa 
      catagtagg ctatgtcat gagcatggt attaaacaa 
      gagagttgt tag
    mRNA sequence
    • >TC006757 RA
      gctcaattg taaatatta ttgtaaaaa aatgtaaac 
      aaataaatt atcgcacaa ttatcgcat tgtttgttt 
      ttatttcaa tacaagtga taagggggt gtcgaaatt 
      gaagacccc tgacgtcac aggccattt tattattga 
      tttttcata gataggtta tgttgaggg ggtgttttg 
      agcccccaa cgctcgtaa acgcgcaaa tgggcgagt 
      tccaagaca cccccaaga cagaacaac ccccagttt 
      ttgaaaacg ttcaaatta agaccgaaa ccgagcaca 
      attctagtc ctgagggcg agtccgacg aggatttcg 
      agctgaagc tcgttaaaa gtgaagatt tcgagcgca 
      acaacgatg cactggata acgaaacca cacggtata 
      agtgcaaca tctgttcgc tggctttca aagttttgg 
      cggactatc gaacgcacc ggaagcaac attttgtgg 
      aaaaaagga catgtaagg tgtgccata cagtgtgcc 
      aaagcatca gtaaactcg agatacata tgaacacgc 
      atttggggc tcaagccgt acaagtgca cagtctgcg 
      ccaaaaatt tcgtctcaa gtaatcatc taaaactgc 
      acgggaggt gccattcgg atgagaaac gatactctt 
      gccagaaat gcagcaagg cgtttcggt acttggggt 
      ccctgagga gtcacatgg tcacacaca acgacatca 
      aggagtttg agtgtaagc actgtggcg ccaactctc 
      gcaacgttg aagcgcacc gtaaacacc tccaatccc 
      acggccaaa aaatcgaaa tcaagtgcg agctctgca 
      accggaact ttgcaaccg aacgcctac tgcaaaccc 
      acctaaact cgcactctg agttgcaat acccttgcg 
      aatactgcg gccgcatct acccctcgt tgtatcgcc 
      tcaaacgcc acataaaac gcgcacaca ttcccaacg 
      tttgcgacg aatgcaacg ctgttttct acgatagag 
      ctctattca cacgccaca agaaacaac acaacgagg 
      gcaagcccg tatcgtgtt cagtttgtg gcaaaaact 
      tcgacaaac agaagaact tgagcgaac atgtgcgcc 
      tccaacaca aggaggacg gtaatgtcc ataaatgtg 
      acttgtgcg agaaaattt ttatcaacg catcgctgc 
      ttcgcaacc atatcaaga cgcatgata agtgcttta 
      aatgcccgt tttgcgcta aattgttca gttcgaggt 
      ataatctcg acacgcatc tagtgacac acactgggg 
      agaggaacc acaagtgcg acatctgtg ggaatgtgt 
      actcgacga agaccagcc ttaagaacc acaaggcga 
      ctcatagtg atgccagaa atttcaagt gtgaccaat 
      gccccaaat tattcaaaa ctaatcgga gattatacg 
      ttcataaat tttcgcatg ccactgaag aaaagttcg 
      agtgtgggg tttgtaatg cgaggttta aagtcaaac 
      agtacttga aatatcaca tgatcaagc attcgagca 
      ataagccgt tcgaatgca aagtctgtc aaaaaaaat 
      tcaagcaca agaagtcgt gggagaaac attcgagtc 
      acgacaaac acttgcgaa acgttaaag aggaggcgc 
      aaaattgtg gcgtttgtg ggcacgtgt gtgtcacta 
      aaaccgacc tgctggaac atagtaggc tatgtcatg 
      agcatggta ttaaacaag agagttgtt agttttgtt 
      ccttttgtg ttgtgatat ttggtgtga taataaatc 
      ctgtacttg gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY