Status
Please wait ...
Gene information
General information
TC001724 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8497108..8529651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001724
    iBeetle screen iB_07574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001724 RA
      MVTVDNFTS KLCAIILGK NTEDVANEW RLLGQEQDE 
      TLLFSWVQS SKIQKDVTK IGLYSFSTN DLTVIHTFE 
      TVVNCIQAS IDSSKTYLA YVIKRIDPN DSKIFTYNP 
      YLLKIGTKN EICDLDLER SKQILVQFL YQKYSVLSE 
      NSPVKFLIL IHQECILQY QIKNDNELN AENFFYESL 
      VRKFSWAQW DPIHQTLYY IHNRKPTRC LVEGEEETP 
      PSLTSKTSP TLSGLQFHD DLPHESVLN IPLNLPHLS 
      TVSGNCGIY EDDSVPLRI HDCSLDLIV LTNSNGYVC 
      VCHHYLYQP IQPTTELKD DDTSLVHFA YSVTVLHQS 
      CVIHCVVPG LPWSKAKTI RPLFKYHGD HMLVFIPEV 
      CTHLLDIGA MHEPCCHVI TKPLLTNLE THRLCLTSL 
      VTVGSDYVI NLATLDLID MTMPTNLLV QTFKNNPHL 
      GNKLSIIHY LILHRSEME IVSELIAWQ AEKPLSLGL 
      PQFLREFLI GSSYASVQR NLPSDASHL VNLLPITTQ 
      HMGNEFEVR INDKMICLS QDVLWNASM MLLSPQQRI 
      VPYRSDIWV KLWEQLAKI SKGKQRFRP TQVVEKLLV 
      SLVCYQPEA LSRSSTPMS PTGSSAVLN DFLSIGNQC 
      GRKLLDALP FYEIESCTA SKQEHIISV NLRELSMHL 
      LKQSAENKI SKFQWQSQT PMHVHAVAT RYITAQLEQ 
      SRYLCQILC KAATFDPRQ ELEKGFVYI EQLQEERRY 
      ILFTILERY YLSVESIAF PLPQGFTSF FAYLGYKTL 
      SFDMFLQYI QRNVLELQV DVMKIIMAG EFDLRLKEN 
      TF
    CDS sequence
    • >TC001724 RA
      atggtgaca gtggataat tttacctca aaattgtgc 
      gctataatt ttagggaaa aatactgaa gatgtagca 
      aatgagtgg cgtttgctg ggccaagaa caagatgag 
      actttactc ttcagttgg gtacaatcg agcaagatt 
      caaaaagac gttactaaa atcggatta tactcattt 
      tcgacaaat gacttaact gtaatacat acctttgaa 
      actgtagtt aattgtatt caagcttcc atcgatagt 
      agtaaaact taccttgcc tatgttatt aaaagaatc 
      gatccaaat gatagcaaa attttcaca tacaatccc 
      tatttatta aaaattggc acaaaaaac gaaatctgt 
      gatttggac cttgaacgg tccaaacaa atattggta 
      cagtttctc taccaaaaa tattccgtt ctatcggaa 
      aattctcca gtgaaattt cttatttta atccaccag 
      gaatgcatt ttgcaatat cagataaaa aatgataac 
      gagttgaac gcagaaaat ttcttttac gaatcgcta 
      gtgcgaaaa tttagttgg gcccagtgg gacccaatc 
      catcaaacc ctttactac atacacaac cggaaacct 
      acgagatgt ctagtcgaa ggcgaagaa gaaacacct 
      ccaagcctc acaagcaaa acatcaccg actttgtcg 
      ggattacaa ttccatgat gacctgcca catgaaagt 
      gtgctcaac atcccgctg aatttaccc cacctatcg 
      actgtctcg ggcaattgt ggcatttac gaagacgat 
      tctgtccct ttacgcatt cacgattgt tcccttgat 
      ttaattgtt ttgaccaat tcaaacggt tacgtttgc 
      gtgtgccac cactactta tatcaacca atacagccc 
      acaactgaa ctcaaagat gacgatacg agtttagtc 
      cattttgct tattctgtt actgtgtta caccaaagc 
      tgtgtcata cattgtgta gttcctgga cttccctgg 
      agtaaagcg aaaacaata cgccctcta tttaaatac 
      cacggtgat catatgctg gtttttata ccagaagtg 
      tgtacacat ttattggat attggagca atgcatgag 
      ccctgttgt catgtgata acaaaacca ttattaacc 
      aacttagaa acgcacaga ctttgtctt acatcactt 
      gtgaccgta gggagcgat tatgtgatt aatttagcg 
      actttagat ttaattgac atgacaatg ccaacaaat 
      ttgcttgtg caaacgttt aaaaataat cctcacctc 
      ggaaataaa ttgtcaata atacattat cttattttg 
      catcgaagt gaaatggag atcgtttcg gagttaatt 
      gcatggcag gctgagaag cctttgagt ttgggtttg 
      ccacaattc ttgagggaa tttttgata ggaagttcg 
      tacgcttca gtacagagg aacttgccg tctgatgcc 
      agccactta gtgaattta ttaccaata actacccaa 
      catatgggt aatgaattc gaagtaagg attaatgat 
      aagatgata tgtttatca caagacgtc ctttggaat 
      gcttctatg atgttgctt tcgccacag caacgcata 
      gttccgtac agaagtgac atttgggta aaactttgg 
      gaacaactc gcaaaaatt tcaaaagga aagcaacga 
      tttcggcct actcaagtc gtcgaaaaa ttattagtg 
      tccctcgtg tgctaccag ccagaagcg ctgtcaaga 
      tctagcact ccaatgtct ccgactggc tcatccgca 
      gtcctaaac gactttctt tcaattgga aatcaatgc 
      ggccgaaaa ttgctcgat gcgttaccg ttttatgaa 
      atcgaaagc tgtacagcg agtaaacaa gaacacatc 
      atctcagtt aatttgcgt gaactcagt atgcattta 
      ttgaaacaa agcgctgaa aacaaaatc agcaaattt 
      cagtggcaa tcacaaact ccaatgcac gtgcatgcc 
      gttgccaca agatacatc acagctcaa ctcgaacag 
      tcgcgatat ttgtgccaa attttgtgc aaagccgct 
      acttttgac ccgcgacag gagctggaa aaaggattt 
      gtttacatt gagcaatta caagaagaa aggcgatat 
      attttgttc acaatactc gaacgatat tacctatct 
      gtagaatca attgcattt cccctacct caaggtttt 
      acttcgttt tttgcatat ttgggctac aaaacccta 
      agctttgat atgtttctg cagtacatt caacgaaat 
      gttcttgag ttacaggtg gatgttatg aaaattatt 
      atggcaggt gagtttgac ttaagatta aaagagaac 
      acgttttag
    mRNA sequence
    • >TC001724 RA
      aacctgccc ttttttcta aatttgcaa tttttccaa 
      attttgcga tgcgataag taaatttgt gaacacata 
      caacgtagt aggcttgtg atggtgaca gtggataat 
      tttacctca aaattgtgc gctataatt ttagggaaa 
      aatactgaa gatgtagca aatgagtgg cgtttgctg 
      ggccaagaa caagatgag actttactc ttcagttgg 
      gtacaatcg agcaagatt caaaaagac gttactaaa 
      atcggatta tactcattt tcgacaaat gacttaact 
      gtaatacat acctttgaa actgtagtt aattgtatt 
      caagcttcc atcgatagt agtaaaact taccttgcc 
      tatgttatt aaaagaatc gatccaaat gatagcaaa 
      attttcaca tacaatccc tatttatta aaaattggc 
      acaaaaaac gaaatctgt gatttggac cttgaacgg 
      tccaaacaa atattggta cagtttctc taccaaaaa 
      tattccgtt ctatcggaa aattctcca gtgaaattt 
      cttatttta atccaccag gaatgcatt ttgcaatat 
      cagataaaa aatgataac gagttgaac gcagaaaat 
      ttcttttac gaatcgcta gtgcgaaaa tttagttgg 
      gcccagtgg gacccaatc catcaaacc ctttactac 
      atacacaac cggaaacct acgagatgt ctagtcgaa 
      ggcgaagaa gaaacacct ccaagcctc acaagcaaa 
      acatcaccg actttgtcg ggattacaa ttccatgat 
      gacctgcca catgaaagt gtgctcaac atcccgctg 
      aatttaccc cacctatcg actgtctcg ggcaattgt 
      ggcatttac gaagacgat tctgtccct ttacgcatt 
      cacgattgt tcccttgat ttaattgtt ttgaccaat 
      tcaaacggt tacgtttgc gtgtgccac cactactta 
      tatcaacca atacagccc acaactgaa ctcaaagat 
      gacgatacg agtttagtc cattttgct tattctgtt 
      actgtgtta caccaaagc tgtgtcata cattgtgta 
      gttcctgga cttccctgg agtaaagcg aaaacaata 
      cgccctcta tttaaatac cacggtgat catatgctg 
      gtttttata ccagaagtg tgtacacat ttattggat 
      attggagca atgcatgag ccctgttgt catgtgata 
      acaaaacca ttattaacc aacttagaa acgcacaga 
      ctttgtctt acatcactt gtgaccgta gggagcgat 
      tatgtgatt aatttagcg actttagat ttaattgac 
      atgacaatg ccaacaaat ttgcttgtg caaacgttt 
      aaaaataat cctcacctc ggaaataaa ttgtcaata 
      atacattat cttattttg catcgaagt gaaatggag 
      atcgtttcg gagttaatt gcatggcag gctgagaag 
      cctttgagt ttgggtttg ccacaattc ttgagggaa 
      tttttgata ggaagttcg tacgcttca gtacagagg 
      aacttgccg tctgatgcc agccactta gtgaattta 
      ttaccaata actacccaa catatgggt aatgaattc 
      gaagtaagg attaatgat aagatgata tgtttatca 
      caagacgtc ctttggaat gcttctatg atgttgctt 
      tcgccacag caacgcata gttccgtac agaagtgac 
      atttgggta aaactttgg gaacaactc gcaaaaatt 
      tcaaaagga aagcaacga tttcggcct actcaagtc 
      gtcgaaaaa ttattagtg tccctcgtg tgctaccag 
      ccagaagcg ctgtcaaga tctagcact ccaatgtct 
      ccgactggc tcatccgca gtcctaaac gactttctt 
      tcaattgga aatcaatgc ggccgaaaa ttgctcgat 
      gcgttaccg ttttatgaa atcgaaagc tgtacagcg 
      agtaaacaa gaacacatc atctcagtt aatttgcgt 
      gaactcagt atgcattta ttgaaacaa agcgctgaa 
      aacaaaatc agcaaattt cagtggcaa tcacaaact 
      ccaatgcac gtgcatgcc gttgccaca agatacatc 
      acagctcaa ctcgaacag tcgcgatat ttgtgccaa 
      attttgtgc aaagccgct acttttgac ccgcgacag 
      gagctggaa aaaggattt gtttacatt gagcaatta 
      caagaagaa aggcgatat attttgttc acaatactc 
      gaacgatat tacctatct gtagaatca attgcattt 
      cccctacct caaggtttt acttcgttt tttgcatat 
      ttgggctac aaaacccta agctttgat atgtttctg 
      cagtacatt caacgaaat gttcttgag ttacaggtg 
      gatgttatg aaaattatt atggcaggt gagtttgac 
      ttaagatta aaagagaac acgttttag tgtgttcct 
      aagccagcg gatagtgcg gaatttatt tttctatta 
      tcttattat tacatataa tttttggaa ttttttttt 
      attaaaatt gttggctac ccgatctct tttaaacac 
      aattgtact taaatatac aatgtgatt taga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY