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Gene information
General information
TC006728 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13442093..13445587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
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    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07571

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC006728 RA
    CDS sequence
    • >TC006728 RA
      atgtctagt cgtgtgtcg ttcatccag gcccgtcgt 
      tacgctaaa gtcctaaat tctaaccta atcaacatt 
      ttgaagacc atgcctaag gagaagaca cacattcca 
      gaggcccaa aaacagcgc cgcaaaatc aaagaaaag 
      ttcagtaaa tacagtcct ggcagagtc acgttgcaa 
      gttttaggg acgggggcc gagggggcc cctagatca 
      ctctatgtt ttttcggac cagtcacga tatttgttc 
      aattgtggc gaaggcaca caacgtttg gcccacgaa 
      cataaaatg aagttggca aaactggaa cacattttt 
      atcacacgg cctacttgg gcgaacatt ggggggctg 
      ccgggggcc gccttgacg atacaagac gtgggggtg 
      cccgaaata accctccat ggcccccaa ggattggag 
      gaaattttt attgccact cgccggttt attgtaatc 
      aaagacttg gacataaaa atggcccag tgtgacgaa 
      aatacaact ttcgaagac aacgttttg aaagttaaa 
      tacgtgcct cttaaacgt agcggaaat tcctcccca 
      atttcagaa ccaaaaaat tcaaatttg ggccaaatg 
      caggaagac aatattgat tattacgcc catgaacat 
      agtggtcgg cgcaaaagc ccgccaaaa atctccgaa 
      tcaaaatta cttcaacaa aaaaacaaa ccaacaaat 
      ttcagcatg gcctatatt tgccagttg cagccccgt 
      ccgggggct ctattgcta gacaagtgt gtcagtttt 
      ggggtgccc ccgggcccc cttttgggg caactaaaa 
      tcaggtcat gacgtcact cttgtcaat ggaaaggtg 
      gtcaaagcg tcagacgtt tgtgaacct gacgatccc 
      ggtcctgtt tttatagtg cttgaatgc ccgtcagag 
      gaatattta gattcactt attgattgt gaagaattt 
      aaaaaacat caagaaacg gcaacaaaa gacgaggat 
      ctggcgcac acagtcgtt catttcacg ccgcaaaaa 
      ataataaat catccaaga tacaaagaa tgggtcgat 
      aaattttcc ccaagcacg caccattta attttgaac 
      gacagcaac acttgcatg ggcagttca gcagttcat 
      cgaattcaa tacaaacta aacctttta tctgatgat 
      attttcccg cttttgggc gacaaaggc actcaaaca 
      attgacgat caagtgaca gagccccct caacccaaa 
      aaaatcaaa cccgacagt ccttccctc ccgattgac 
      caactgtcg gtaaagtcg cgacctgca tcacccaca 
      gaccccaac atttacaca aacacactt ttcaattac 
      catctgcga ccgaaaaaa gcctgcgat cgttcaaat 
      gaattaaaa ctagtgcct tcgcaattt attgacgaa 
      acaatggcc attgaaaat ttcccctca gtgttacac 
      gaagtgacg caacagatg gcgcaaaaa cggcgccat 
      ttaacggtc cgggatttc ccccgatta ttgtttcta 
      gggacggga tcgtcgatt ccgaacaag acgcgcaat 
      acgagcggg atgttgctc gaaattgac gaaaataca 
      agtattgtg attgattgt ggggaaggt acggtgggt 
      caaatcatt aggttttat gggcaagac aaggcaaat 
      gccgagctg gctaaaata agggcggtt tatatttct 
      catttgcac gccgatcat cacttaggg ttaattggg 
      gtgttgcaa gggcgccaa agggcgatg caaaaattg 
      gaaaagggg gcgcaaccc ttgtttttg ttagccccc 
      aaacaaatc atgtggtgg ctcaatttc tacgaccgc 
      tgttttgaa aaaataagc gaggagttt gttttggtc 
      cctaatggc gaactgttt tttaataat cacgaactg 
      gaccaactt ttggaaaaa cagttgtta gaagggctc 
      cagatgcgt gatataagc acttgtctg gtcaagcat 
      tgccctaat gccttcggt gtcagtttt gtgcaccaa 
      aatgggtac aaaataacc tactctggt gacacaatg 
      ccgtcggaa aacctcgta cagttgggc aaaaccagc 
      gatttgttg atacacgaa gccacaatg gaggatgaa 
      ttagccgca gaggctgtc attaaaatg cactcaacg 
      acgtcacaa gcgatcgaa atcgggcgc caaatggag 
      gcgaaatac actctcttg actcatttt agccaaaga 
      tatgcgaag ttgcccagg tttaacgag aatttttcg 
      gacaacgtt ggcatcgcc ttcgataac atgcgagtg 
      aaactggac gaattaccg ctgattccc ctcctgtat 
      ccggccctt aaattaatg tttgtggaa tactacgaa 
      gagatggag caaaaggcg gccaagcgc caaatgaaa 
      atcgaacgg gagaaagcc cagcaattt gtaacataa
    mRNA sequence
    • >TC006728 RA
      agtgtcaca atcacactt ccaatttca aaatttcca 
      atatttcac catttttcc cattgattt catcagttc 
      tcatcaaaa actaatgtc tagtcgtgt gtcgttcat 
      ccaggcccg tcgttacgc taaagtcct aaattctaa 
      cctaatcaa cattttgaa gaccatgcc taaggagaa 
      gacacacat tccagaggc ccaaaaaca gcgccgcaa 
      aatcaaaga aaagttcag taaatacag tcctggcag 
      agtcacgtt gcaagtttt agggacggg ggccgaggg 
      ggcccctag atcactcta tgttttttc ggaccagtc 
      acgatattt gttcaattg tggcgaagg cacacaacg 
      tttggccca cgaacataa aatgaagtt ggcaaaact 
      ggaacacat ttttatcac acggcctac ttgggcgaa 
      cattggggg gctgccggg ggccgcctt gacgataca 
      agacgtggg ggtgcccga aataaccct ccatggccc 
      ccaaggatt ggaggaaat ttttattgc cactcgccg 
      gtttattgt aatcaaaga cttggacat aaaaatggc 
      ccagtgtga cgaaaatac aactttcga agacaacgt 
      tttgaaagt taaatacgt gcctcttaa acgtagcgg 
      aaattcctc cccaatttc agaaccaaa aaattcaaa 
      tttgggcca aatgcagga agacaatat tgattatta 
      cgcccatga acatagtgg tcggcgcaa aagcccgcc 
      aaaaatctc cgaatcaaa attacttca acaaaaaaa 
      caaaccaac aaatttcag catggccta tatttgcca 
      gttgcagcc ccgtccggg ggctctatt gctagacaa 
      gtgtgtcag ttttggggt gcccccggg ccccctttt 
      ggggcaact aaaatcagg tcatgacgt cactcttgt 
      caatggaaa ggtggtcaa agcgtcaga cgtttgtga 
      acctgacga tcccggtcc tgtttttat agtgcttga 
      atgcccgtc agaggaata tttagattc acttattga 
      ttgtgaaga atttaaaaa acatcaaga aacggcaac 
      aaaagacga ggatctggc gcacacagt cgttcattt 
      cacgccgca aaaaataat aaatcatcc aagatacaa 
      agaatgggt cgataaatt ttccccaag cacgcacca 
      tttaatttt gaacgacag caacacttg catgggcag 
      ttcagcagt tcatcgaat tcaatacaa actaaacct 
      tttatctga tgatatttt cccgctttt gggcgacaa 
      aggcactca aacaattga cgatcaagt gacagagcc 
      ccctcaacc caaaaaaat caaacccga cagtccttc 
      cctcccgat tgaccaact gtcggtaaa gtcgcgacc 
      tgcatcacc cacagaccc caacattta cacaaacac 
      acttttcaa ttaccatct gcgaccgaa aaaagcctg 
      cgatcgttc aaatgaatt aaaactagt gccttcgca 
      atttattga cgaaacaat ggccattga aaatttccc 
      ctcagtgtt acacgaagt gacgcaaca gatggcgca 
      aaaacggcg ccatttaac ggtccggga tttcccccg 
      attattgtt tctagggac gggatcgtc gattccgaa 
      caagacgcg caatacgag cgggatgtt gctcgaaat 
      tgacgaaaa tacaagtat tgtgattga ttgtgggga 
      aggtacggt gggtcaaat cattaggtt ttatgggca 
      agacaaggc aaatgccga gctggctaa aataagggc 
      ggtttatat ttctcattt gcacgccga tcatcactt 
      agggttaat tggggtgtt gcaagggcg ccaaagggc 
      gatgcaaaa attggaaaa gggggcgca acccttgtt 
      tttgttagc ccccaaaca aatcatgtg gtggctcaa 
      tttctacga ccgctgttt tgaaaaaat aagcgagga 
      gtttgtttt ggtccctaa tggcgaact gttttttaa 
      taatcacga actggacca acttttgga aaaacagtt 
      gttagaagg gctccagat gcgtgatat aagcacttg 
      tctggtcaa gcattgccc taatgcctt cggtgtcag 
      ttttgtgca ccaaaatgg gtacaaaat aacctactc 
      tggtgacac aatgccgtc ggaaaacct cgtacagtt 
      gggcaaaac cagcgattt gttgataca cgaagccac 
      aatggagga tgaattagc cgcagaggc tgtcattaa 
      aatgcactc aacgacgtc acaagcgat cgaaatcgg 
      gcgccaaat ggaggcgaa atacactct cttgactca 
      ttttagcca aagatatgc gaagttgcc caggtttaa 
      cgagaattt ttcggacaa cgttggcat cgccttcga 
      taacatgcg agtgaaact ggacgaatt accgctgat 
      tcccctcct gtatccggc ccttaaatt aatgtttgt 
      ggaatacta cgaagagat ggagcaaaa ggcggccaa 
      gcgccaaat gaaaatcga acgggagaa agcccagca 
      atttgtaac ataaagatt gattaaacg gctgtttgt 
      aacattttt ttgattttg tcccttttt tgaaatgtc 

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY