| General information | |||
| TC006728 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13442093..13445587 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006728 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07571 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC006728 RA
MSSRVSFIQ ARRYAKVLN SNLINILKT MPKEKTHIP EAQKQRRKI KEKFSKYSP GRVTLQVLG TGAEGAPRS LYVFSDQSR YLFNCGEGT QRLAHEHKM KLAKLEHIF ITRPTWANI GGLPGAALT IQDVGVPEI TLHGPQGLE EIFIATRRF IVIKDLDIK MAQCDENTT FEDNVLKVK YVPLKRSGN SSPISEPKN SNLGQMQED NIDYYAHEH SGRRKSPPK ISESKLLQQ KNKPTNFSM AYICQLQPR PGALLLDKC VSFGVPPGP LLGQLKSGH DVTLVNGKV VKASDVCEP DDPGPVFIV LECPSEEYL DSLIDCEEF KKHQETATK DEDLAHTVV HFTPQKIIN HPRYKEWVD KFSPSTHHL ILNDSNTCM GSSAVHRIQ YKLNLLSDD IFPLLGDKG TQTIDDQVT EPPQPKKIK PDSPSLPID QLSVKSRPA SPTDPNIYT NTLFNYHLR PKKACDRSN ELKLVPSQF IDETMAIEN FPSVLHEVT QQMAQKRRH LTVRDFPRL LFLGTGSSI PNKTRNTSG MLLEIDENT SIVIDCGEG TVGQIIRFY GQDKANAEL AKIRAVYIS HLHADHHLG LIGVLQGRQ RAMQKLEKG AQPLFLLAP KQIMWWLNF YDRCFEKIS EEFVLVPNG ELFFNNHEL DQLLEKQLL EGLQMRDIS TCLVKHCPN AFGVSFVHQ NGYKITYSG DTMPSENLV QLGKTSDLL IHEATMEDE LAAEAVIKM HSTTSQAIE IGRQMEAKY TLLTHFSQR YAKLPRFNE NFSDNVGIA FDNMRVKLD ELPLIPLLY PALKLMFVE YYEEMEQKA AKRQMKIER EKAQQFVT
- >TC006728 RA
atgtctagt cgtgtgtcg ttcatccag gcccgtcgt tacgctaaa gtcctaaat tctaaccta atcaacatt ttgaagacc atgcctaag gagaagaca cacattcca gaggcccaa aaacagcgc cgcaaaatc aaagaaaag ttcagtaaa tacagtcct ggcagagtc acgttgcaa gttttaggg acgggggcc gagggggcc cctagatca ctctatgtt ttttcggac cagtcacga tatttgttc aattgtggc gaaggcaca caacgtttg gcccacgaa cataaaatg aagttggca aaactggaa cacattttt atcacacgg cctacttgg gcgaacatt ggggggctg ccgggggcc gccttgacg atacaagac gtgggggtg cccgaaata accctccat ggcccccaa ggattggag gaaattttt attgccact cgccggttt attgtaatc aaagacttg gacataaaa atggcccag tgtgacgaa aatacaact ttcgaagac aacgttttg aaagttaaa tacgtgcct cttaaacgt agcggaaat tcctcccca atttcagaa ccaaaaaat tcaaatttg ggccaaatg caggaagac aatattgat tattacgcc catgaacat agtggtcgg cgcaaaagc ccgccaaaa atctccgaa tcaaaatta cttcaacaa aaaaacaaa ccaacaaat ttcagcatg gcctatatt tgccagttg cagccccgt ccgggggct ctattgcta gacaagtgt gtcagtttt ggggtgccc ccgggcccc cttttgggg caactaaaa tcaggtcat gacgtcact cttgtcaat ggaaaggtg gtcaaagcg tcagacgtt tgtgaacct gacgatccc ggtcctgtt tttatagtg cttgaatgc ccgtcagag gaatattta gattcactt attgattgt gaagaattt aaaaaacat caagaaacg gcaacaaaa gacgaggat ctggcgcac acagtcgtt catttcacg ccgcaaaaa ataataaat catccaaga tacaaagaa tgggtcgat aaattttcc ccaagcacg caccattta attttgaac gacagcaac acttgcatg ggcagttca gcagttcat cgaattcaa tacaaacta aacctttta tctgatgat attttcccg cttttgggc gacaaaggc actcaaaca attgacgat caagtgaca gagccccct caacccaaa aaaatcaaa cccgacagt ccttccctc ccgattgac caactgtcg gtaaagtcg cgacctgca tcacccaca gaccccaac atttacaca aacacactt ttcaattac catctgcga ccgaaaaaa gcctgcgat cgttcaaat gaattaaaa ctagtgcct tcgcaattt attgacgaa acaatggcc attgaaaat ttcccctca gtgttacac gaagtgacg caacagatg gcgcaaaaa cggcgccat ttaacggtc cgggatttc ccccgatta ttgtttcta gggacggga tcgtcgatt ccgaacaag acgcgcaat acgagcggg atgttgctc gaaattgac gaaaataca agtattgtg attgattgt ggggaaggt acggtgggt caaatcatt aggttttat gggcaagac aaggcaaat gccgagctg gctaaaata agggcggtt tatatttct catttgcac gccgatcat cacttaggg ttaattggg gtgttgcaa gggcgccaa agggcgatg caaaaattg gaaaagggg gcgcaaccc ttgtttttg ttagccccc aaacaaatc atgtggtgg ctcaatttc tacgaccgc tgttttgaa aaaataagc gaggagttt gttttggtc cctaatggc gaactgttt tttaataat cacgaactg gaccaactt ttggaaaaa cagttgtta gaagggctc cagatgcgt gatataagc acttgtctg gtcaagcat tgccctaat gccttcggt gtcagtttt gtgcaccaa aatgggtac aaaataacc tactctggt gacacaatg ccgtcggaa aacctcgta cagttgggc aaaaccagc gatttgttg atacacgaa gccacaatg gaggatgaa ttagccgca gaggctgtc attaaaatg cactcaacg acgtcacaa gcgatcgaa atcgggcgc caaatggag gcgaaatac actctcttg actcatttt agccaaaga tatgcgaag ttgcccagg tttaacgag aatttttcg gacaacgtt ggcatcgcc ttcgataac atgcgagtg aaactggac gaattaccg ctgattccc ctcctgtat ccggccctt aaattaatg tttgtggaa tactacgaa gagatggag caaaaggcg gccaagcgc caaatgaaa atcgaacgg gagaaagcc cagcaattt gtaacataa
- >TC006728 RA
agtgtcaca atcacactt ccaatttca aaatttcca atatttcac catttttcc cattgattt catcagttc tcatcaaaa actaatgtc tagtcgtgt gtcgttcat ccaggcccg tcgttacgc taaagtcct aaattctaa cctaatcaa cattttgaa gaccatgcc taaggagaa gacacacat tccagaggc ccaaaaaca gcgccgcaa aatcaaaga aaagttcag taaatacag tcctggcag agtcacgtt gcaagtttt agggacggg ggccgaggg ggcccctag atcactcta tgttttttc ggaccagtc acgatattt gttcaattg tggcgaagg cacacaacg tttggccca cgaacataa aatgaagtt ggcaaaact ggaacacat ttttatcac acggcctac ttgggcgaa cattggggg gctgccggg ggccgcctt gacgataca agacgtggg ggtgcccga aataaccct ccatggccc ccaaggatt ggaggaaat ttttattgc cactcgccg gtttattgt aatcaaaga cttggacat aaaaatggc ccagtgtga cgaaaatac aactttcga agacaacgt tttgaaagt taaatacgt gcctcttaa acgtagcgg aaattcctc cccaatttc agaaccaaa aaattcaaa tttgggcca aatgcagga agacaatat tgattatta cgcccatga acatagtgg tcggcgcaa aagcccgcc aaaaatctc cgaatcaaa attacttca acaaaaaaa caaaccaac aaatttcag catggccta tatttgcca gttgcagcc ccgtccggg ggctctatt gctagacaa gtgtgtcag ttttggggt gcccccggg ccccctttt ggggcaact aaaatcagg tcatgacgt cactcttgt caatggaaa ggtggtcaa agcgtcaga cgtttgtga acctgacga tcccggtcc tgtttttat agtgcttga atgcccgtc agaggaata tttagattc acttattga ttgtgaaga atttaaaaa acatcaaga aacggcaac aaaagacga ggatctggc gcacacagt cgttcattt cacgccgca aaaaataat aaatcatcc aagatacaa agaatgggt cgataaatt ttccccaag cacgcacca tttaatttt gaacgacag caacacttg catgggcag ttcagcagt tcatcgaat tcaatacaa actaaacct tttatctga tgatatttt cccgctttt gggcgacaa aggcactca aacaattga cgatcaagt gacagagcc ccctcaacc caaaaaaat caaacccga cagtccttc cctcccgat tgaccaact gtcggtaaa gtcgcgacc tgcatcacc cacagaccc caacattta cacaaacac acttttcaa ttaccatct gcgaccgaa aaaagcctg cgatcgttc aaatgaatt aaaactagt gccttcgca atttattga cgaaacaat ggccattga aaatttccc ctcagtgtt acacgaagt gacgcaaca gatggcgca aaaacggcg ccatttaac ggtccggga tttcccccg attattgtt tctagggac gggatcgtc gattccgaa caagacgcg caatacgag cgggatgtt gctcgaaat tgacgaaaa tacaagtat tgtgattga ttgtgggga aggtacggt gggtcaaat cattaggtt ttatgggca agacaaggc aaatgccga gctggctaa aataagggc ggtttatat ttctcattt gcacgccga tcatcactt agggttaat tggggtgtt gcaagggcg ccaaagggc gatgcaaaa attggaaaa gggggcgca acccttgtt tttgttagc ccccaaaca aatcatgtg gtggctcaa tttctacga ccgctgttt tgaaaaaat aagcgagga gtttgtttt ggtccctaa tggcgaact gttttttaa taatcacga actggacca acttttgga aaaacagtt gttagaagg gctccagat gcgtgatat aagcacttg tctggtcaa gcattgccc taatgcctt cggtgtcag ttttgtgca ccaaaatgg gtacaaaat aacctactc tggtgacac aatgccgtc ggaaaacct cgtacagtt gggcaaaac cagcgattt gttgataca cgaagccac aatggagga tgaattagc cgcagaggc tgtcattaa aatgcactc aacgacgtc acaagcgat cgaaatcgg gcgccaaat ggaggcgaa atacactct cttgactca ttttagcca aagatatgc gaagttgcc caggtttaa cgagaattt ttcggacaa cgttggcat cgccttcga taacatgcg agtgaaact ggacgaatt accgctgat tcccctcct gtatccggc ccttaaatt aatgtttgt ggaatacta cgaagagat ggagcaaaa ggcggccaa gcgccaaat gaaaatcga acgggagaa agcccagca atttgtaac ataaagatt gattaaacg gctgtttgt aacattttt ttgattttg tcccttttt tgaaatgtc aa
