Status
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Gene information
General information
TC031567
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001731,TC001730
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8645911..8666003
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031567
      iBeetle screen iB_07566

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031567 RA
        MDEGFRSPM GRKGCRTGR RKTGKKFLT NQNLCSQKL 
        KNEMTTKTG MMKIIVTRQ NIPTDPILE IHDEGSLAS 
        SITIDPQLI SDIDFSEIE KQFKVHVDE DSSDAFTNQ 
        PQSNRGKGR GMGKGKGKQ LYKKSPSMC RAVSPSPSS 
        TFSLIDEDE TMEMTTEVD RMTNVITLF NVLVLPRGC 
        DESEVRLGW LSALPIAQT STLDFIMAP TRGRKRQAE 
        SRAESPVPV RAGQPDENL VALVTPVQS PDTTVTGGD 
        AIPYFDPSN TEFNSERWC NKVDECRAV FHWTEETTV 
        YFAITKLKG MAEVWYRSL PTMMRTWDE WKIQLQTAF 
        PAQTDYYSM LKRMMARKK KIDEDYTTY YYSKLALLN 
        ELEITGKNS VSCIIGGIT DAIVKTGAK AGQYQTPES 
        LLQYLCSVT ENQTSTTQA GKGRHFSNP RFRPKASFG 
        KNNAKSSRE LICFKCQKP GHTANVCSA RNREMRCTL 
        CRSHGHVAK DCRNKTTNK PSDQKTVAL IGCFNGQLT 
        RAYIDLGSS CTTITNKEI QRLKITKVD TTQISTLRG 
        YGNGTVTTM GTVKITLKI DAVQSEIIV DVVSEGVQN 
        VPVLVGRNF TELTNVGIL KDDVSLRFF TLPPMETRS 
        VQAAKVKLF VAKETIIPP MHYGHMPVR SDIQNGEIF 
        VDYSLRLEP EHEYEIPSV VLQLRENAC VKLPVINFS 
        KNEIRFKKN TTIARGRIC REEKYVPTI LRISKTPQP 
        ELPLDQLDV GNVRPQTKQ HLIDMLSEY RDCFALTVN 
        ELGCAKNVE MEIRLEEEK PFTFRPYRL APSEVEKVN 
        EMITELIQA EIIQESESN YASPIYEYL RMPFGLTNA 
        PRVFQRFMN NLLRPVSKI AAVYLDDVL LHSNTEGQA 
        LCDLREVLD VLRAEGLTL NFQKCAFLK ETVHFLGFE 
        VSDGIIRPG LDKIQAVKN FSPPKNVKQ IRQFIGLTG 
        YFRHFVKNY ALIARPLTN LTRKGVNWK WDTEEELAF 
        ERLKEILTS RPVLSIYDP TAVTELHTD ASSLGVAGI 
        LLQYQTDGR LHPIAYYSR QTNEHERHY HSFELETLA 
        VVESVKKFR IYLLDLEFT IVTDCNELK ATSNKSQLI 
        PRIARWWLQ LLEFRFKVK YRAGTQMSH VDALSRNPN 
        DSKTSKDVL ALDKADWVL AGQLTDPKI KELHNILSK 
        PPSTEYKQN VHKNYALRE ERVYRNTTK GLQWVVPKG 
        MRQQVVRAA HDERGHFAA EKTLSRLSD CYWFPRMRD 
        YVSDYIACC IPCIMKKKP SGKQEGFLH PIPKPTKPF 
        DTIHLDHLG PFPRSKRGN VHIIACIDA CTKFLFMRP 
        VKSTKTVFV VNFLNELYV TYGKPRVVI TDQGSCFTA 
        AKFREHCDQ NQVTHVKVA VATPRANGQ VERLNRSIL 
        SVLMTGTLE DNLWDQNVP MAQFSINNA ANASTGRTP 
        SELMMGYKP RHGTDSYLR DEVQKIPTI VDDLIKLRL 
        EASEKVAQV QKRQKELFD RKRKPPREY KVGDVVVVR 
        KGEAATGES RKLMAPFSQ PMTVKAVLP NDRYLVTDM 
        PNSHRTRRK AIYQRVIAV DRMKPSTKP GGVSDESDG 
        PNEDDQGEP
      CDS sequence
      • >TC031567 RA
        atggatgaa ggatttaga tcgccgatg ggaagaaag 
        ggctgtaga acgggaaga agaaaaacg ggaaaaaaa 
        ttcttaaca aaccaaaac ttgtgttcg cagaagtta 
        aaaaacgag atgacgacg aaaactggg atgatgaag 
        attatcgtc acacgacaa aatatacct actgatccg 
        atcttggaa atacatgat gaaggaagt ctggcgagt 
        tctatcacc atcgatcca caattgatt agtgatatt 
        gactttagt gaaattgag aagcaattt aaggtgcat 
        gtggacgaa gactcatcc gatgccttt accaaccag 
        ccgcagtca aacagggga aaaggtcgg ggaatggga 
        aaaggaaaa ggcaaacaa ctgtacaaa aagtcccct 
        tcgatgtgt cgtgccgtg tcgccatcg ccctcatca 
        acattttcc cttattgat gaggatgaa acgatggag 
        atgactacg gaggtggat aggatgacg aatgttata 
        acgcttttc aatgtcttg gtcttgcca cgtggttgt 
        gatgagagt gaggttagg ctgggctgg ctctccgcc 
        cttcctatt gcacaaacc tcgactctc gattttata 
        atggcaccc accagagga cggaaaaga caggctgaa 
        tctcgtgca gagagccca gtcccagtt cgtgctgga 
        caacccgat gagaatttg gtggcactc gtgacccct 
        gttcaaagc ccagacacc accgtaact gggggtgat 
        gcaatccct tattttgac cccagcaat accgaattt 
        aacagcgaa agatggtgt aacaaagtc gatgagtgt 
        agagctgta ttccactgg accgaagaa actacagtt 
        tatttcgct atcaccaag cttaagggc atggctgaa 
        gtttggtat cgaagccta ccgacaatg atgaggacc 
        tgggatgag tggaaaatt caattgcaa acagctttt 
        cctgcacag acagattat tattcgatg ttgaagcga 
        atgatggcc aggaaaaag aagattgat gaagactac 
        acgacttat tactacagc aaactggca cttttaaac 
        gagctagag ataacgggg aagaacagc gtttcatgc 
        attattggc ggtataact gacgcaatc gtaaaaact 
        ggtgcaaaa gcaggccaa tatcaaacg cccgaatct 
        ctgctgcaa tacctatgc agtgtaacg gagaatcaa 
        acgtctaca acccaagct ggtaagggc cgacatttc 
        tcaaatcca aggttcaga ccaaaagca tcatttggc 
        aaaaataat gctaagtct tcaagagag ctgatttgt 
        ttcaaatgc cagaaaccc ggccataca gctaacgtc 
        tgttcggca cggaaccga gaaatgcgc tgtacactt 
        tgtcgttct catgggcat gtagcaaag gattgtcgg 
        aataagact acgaataaa ccgagtgat cagaaaacc 
        gtagcattg atcggatgt tttaatgga caacttact 
        cgtgcgtac atcgacttg ggaagctcg tgtaccacc 
        attaccaat aaggaaatc caacgatta aaaattacc 
        aaagtggat actacgcag atttccact ttgcggggg 
        tatggcaac gggaccgtc actacaatg ggtaccgtg 
        aaaattact ttaaaaata gatgccgtg caatccgaa 
        ataatcgtt gatgttgtc tcagaaggt gttcagaac 
        gttcctgtt ctggttggg cgcaacttt actgagctg 
        actaatgtg ggcatattg aaagacgat gtgagtctg 
        cgtttcttt actctccct ccgatggaa acgcgttca 
        gtacaagct gcgaaggtg aaattattt gtggctaag 
        gaaactata attcctccc atgcattac gggcacatg 
        ccagttcgt agcgacatt caaaacgga gagattttt 
        gttgactat agcctccga ctggaacct gaacacgag 
        tatgaaata ccaagcgta gttttacaa ctacgagaa 
        aacgcttgt gtaaagctc cctgttata aatttctcc 
        aaaaacgaa attcgtttt aagaagaat acgacgata 
        gccagagga cgaatttgt cgtgaggaa aaatatgtg 
        ccaaccatt ttgagaatt tccaaaact cctcagcca 
        gagttgccg ttggaccag ttagatgtg ggcaatgtc 
        agaccgcaa accaaacaa catttgata gatatgctg 
        tccgaatac cgggattgt ttcgcactt actgtaaat 
        gagttggga tgcgccaaa aacgttgag atggaaatt 
        cgtctagaa gaagaaaaa ccttttaca tttagaccc 
        taccggcta gcacctagc gaggttgaa aaagttaat 
        gaaatgatt actgaacta atacaagct gagattatt 
        caggaatcg gaatcgaat tacgcttca cccatttac 
        gagtacttg cgtatgcca tttggattg acaaatgca 
        cctcgagtt ttccaacgt tttatgaac aatctgctt 
        cgccctgtt tccaaaatt gctgctgtg tatctagat 
        gacgtgtta ttgcattca aataccgaa ggacaagcc 
        ttatgtgat ttgagagaa gtgttggat gttttaaga 
        gctgaaggc ttgacttta aacttccaa aagtgtgcc 
        tttctcaaa gaaaccgtt cactttctc ggatttgaa 
        gtaagcgac ggaataata cgccccggg cttgataag 
        attcaggca gtaaaaaat ttctcccca ccgaaaaat 
        gtcaagcag attagacag tttattgga ctaactggg 
        tactttcga catttcgtc aagaattat gcactcatt 
        gctagacca ttaacaaac ttaacacgg aaaggtgtg 
        aattggaaa tgggacacg gaagaggaa cttgcgttt 
        gagaggctt aaagaaatt ttgacgtcg agaccagtg 
        ttatctatt tatgacccc acagctgtc acagagtta 
        cacacagac gcaagttcg ttgggtgtc gctggtatt 
        ttgctacaa tatcagact gacggcaga ctacaccca 
        atagcctat tacagcagg caaacaaac gagcatgag 
        cgacactac cattcgttc gagcttgaa actttggca 
        gtcgttgaa agtgtcaaa aagtttagg atttacttg 
        ctcgacctt gagtttacg atcgttact gactgtaat 
        gagctgaaa gcaacgtcg aacaagtcc caacttatt 
        ccaagaatc gccaggtgg tggctgcaa ctcctagag 
        tttagattt aaagtaaaa tacagagca ggcactcaa 
        atgagtcat gttgacgcc ttaagtcgc aatcccaat 
        gattccaaa acatctaaa gacgtcttg gctttagat 
        aaagcggat tgggtgctt gcaggacag ttgaccgat 
        cctaaaatc aaggaacta cacaatatt ttgtctaaa 
        ccgccatct accgaatac aagcaaaat gttcataag 
        aactatgca ctccgtgaa gaaagggta tatcgaaac 
        acgacgaaa ggattacag tgggtggta ccaaaaggt 
        atgagacag caagtggtt agggccgct catgacgaa 
        cggggacac tttgccgca gaaaaaacg ttaagccga 
        ctctccgat tgttactgg tttcccaga atgcgggac 
        tacgtctcc gattatatt gcatgctgc atcccgtgc 
        ataatgaaa aagaagccg tcagggaaa caagagggt 
        tttcttcac cctatccca aaaccaacc aagcctttc 
        gatacgata catttggac catctgggg ccatttcca 
        agaagcaaa agaggaaac gtgcacatc attgcgtgc 
        attgatgca tgcacaaaa tttcttttt atgcgtccc 
        gtgaagtca acgaagaca gtttttgtc gtcaacttc 
        ctgaacgag ctgtatgtg acctacggg aagccaagg 
        gtggtaatt accgaccag ggaagctgt ttcactgca 
        gccaagttt agggaacac tgtgatcaa aatcaagtc 
        acacatgta aaggtcgcg gtagcaact cccagagct 
        aatggccaa gtagaacga cttaaccga agcatcctg 
        tcggtgctg atgacgggg accttggaa gacaaccta 
        tgggatcaa aatgttcca atggctcaa ttttctatc 
        aataacgcg gcaaatgct tcgaccgga agaacccca 
        agcgaattg atgatgggg tataaacca cgacacggt 
        actgactcg tatttgaga gatgaggtg cagaagatt 
        ccaacgatt gtggacgat cttattaaa ctgcggtta 
        gaagcctct gaaaaggtg gctcaggta cagaaacgt 
        cagaaggaa ctctttgac agaaaaagg aaaccaccc 
        agagagtat aaagtggga gatgtggta gtcgtccgg 
        aaaggggaa gctgcaacg ggtgagagc cggaaactg 
        atggctcca tttagccag cctatgact gtaaaagcg 
        gtattgcca aacgatcgg tacctagtc acggacatg 
        cccaattca cataggacc cgtaggaag gcaatctat 
        cagcgtgtc atcgccgtg gacaggatg aaaccgtcg 
        actaaacct ggaggagtt tcagacgag tccgatggt 
        cctaacgaa gacgatcaa ggtgaacct taa
      mRNA sequence
      • >TC031567 RA
        ctgttattc tgtggttac gtttcgcgt gcgtgtgtc 
        gttttctcc atttcctct ccaatttcc atctgtaac 
        aaagagggg ctcctatgg ggccgtttg agcgatgaa 
        taccgcatc acgaatcaa ttttggggt tgtttttcc 
        ctcaaaagg aacgaagcg tgtagcact tttgcacaa 
        agaaaaaac aattgtggg gcaccgtcg ataagtcag 
        ttttctttc tgtacttta aaattttgt ggcactttg 
        gcagagaat agactacta cactatttg atcatgaag 
        ggcgaaggg cgaatataa tcattacaa ataccatta 
        tgagcttca attctaact gttagttat tatggtgag 
        gaagatgac attgaacaa tcctttcca taaatgctt 
        ggcccatcg gctttcaaa ccatctatt caaggccca 
        tcatgcaga atggatgaa ggatttaga tcgccgatg 
        ggaagaaag ggctgtaga acgggaaga agaaaaacg 
        ggaaaaaaa ttcttaaca aaccaaaac ttgtgttcg 
        cagaagtta aaaaacgag atgacgacg aaaactggg 
        atgatgaag attatcgtc acacgacaa aatatacct 
        actgatccg atcttggaa atacatgat gaaggaagt 
        ctggcgagt tctatcacc atcgatcca caattgatt 
        agtgatatt gactttagt gaaattgag aagcaattt 
        aaggtgcat gtggacgaa gactcatcc gatgccttt 
        accaaccag ccgcagtca aacagggga aaaggtcgg 
        ggaatggga aaaggaaaa ggcaaacaa ctgtacaaa 
        aagtcccct tcgatgtgt cgtgccgtg tcgccatcg 
        ccctcatca acattttcc cttattgat gaggatgaa 
        acgatggag atgactacg gaggtggat aggatgacg 
        aatgttata acgcttttc aatgtcttg gtcttgcca 
        cgtggttgt gatgagagt gaggttagg ctgggctgg 
        ctctccgcc cttcctatt gcacaaacc tcgactctc 
        gattttata atggcaccc accagagga cggaaaaga 
        caggctgaa tctcgtgca gagagccca gtcccagtt 
        cgtgctgga caacccgat gagaatttg gtggcactc 
        gtgacccct gttcaaagc ccagacacc accgtaact 
        gggggtgat gcaatccct tattttgac cccagcaat 
        accgaattt aacagcgaa agatggtgt aacaaagtc 
        gatgagtgt agagctgta ttccactgg accgaagaa 
        actacagtt tatttcgct atcaccaag cttaagggc 
        atggctgaa gtttggtat cgaagccta ccgacaatg 
        atgaggacc tgggatgag tggaaaatt caattgcaa 
        acagctttt cctgcacag acagattat tattcgatg 
        ttgaagcga atgatggcc aggaaaaag aagattgat 
        gaagactac acgacttat tactacagc aaactggca 
        cttttaaac gagctagag ataacgggg aagaacagc 
        gtttcatgc attattggc ggtataact gacgcaatc 
        gtaaaaact ggtgcaaaa gcaggccaa tatcaaacg 
        cccgaatct ctgctgcaa tacctatgc agtgtaacg 
        gagaatcaa acgtctaca acccaagct ggtaagggc 
        cgacatttc tcaaatcca aggttcaga ccaaaagca 
        tcatttggc aaaaataat gctaagtct tcaagagag 
        ctgatttgt ttcaaatgc cagaaaccc ggccataca 
        gctaacgtc tgttcggca cggaaccga gaaatgcgc 
        tgtacactt tgtcgttct catgggcat gtagcaaag 
        gattgtcgg aataagact acgaataaa ccgagtgat 
        cagaaaacc gtagcattg atcggatgt tttaatgga 
        caacttact cgtgcgtac atcgacttg ggaagctcg 
        tgtaccacc attaccaat aaggaaatc caacgatta 
        aaaattacc aaagtggat actacgcag atttccact 
        ttgcggggg tatggcaac gggaccgtc actacaatg 
        ggtaccgtg aaaattact ttaaaaata gatgccgtg 
        caatccgaa ataatcgtt gatgttgtc tcagaaggt 
        gttcagaac gttcctgtt ctggttggg cgcaacttt 
        actgagctg actaatgtg ggcatattg aaagacgat 
        gtgagtctg cgtttcttt actctccct ccgatggaa 
        acgcgttca gtacaagct gcgaaggtg aaattattt 
        gtggctaag gaaactata attcctccc atgcattac 
        gggcacatg ccagttcgt agcgacatt caaaacgga 
        gagattttt gttgactat agcctccga ctggaacct 
        gaacacgag tatgaaata ccaagcgta gttttacaa 
        ctacgagaa aacgcttgt gtaaagctc cctgttata 
        aatttctcc aaaaacgaa attcgtttt aagaagaat 
        acgacgata gccagagga cgaatttgt cgtgaggaa 
        aaatatgtg ccaaccatt ttgagaatt tccaaaact 
        cctcagcca gagttgccg ttggaccag ttagatgtg 
        ggcaatgtc agaccgcaa accaaacaa catttgata 
        gatatgctg tccgaatac cgggattgt ttcgcactt 
        actgtaaat gagttggga tgcgccaaa aacgttgag 
        atggaaatt cgtctagaa gaagaaaaa ccttttaca 
        tttagaccc taccggcta gcacctagc gaggttgaa 
        aaagttaat gaaatgatt actgaacta atacaagct 
        gagattatt caggaatcg gaatcgaat tacgcttca 
        cccatttac gagtacttg cgtatgcca tttggattg 
        acaaatgca cctcgagtt ttccaacgt tttatgaac 
        aatctgctt cgccctgtt tccaaaatt gctgctgtg 
        tatctagat gacgtgtta ttgcattca aataccgaa 
        ggacaagcc ttatgtgat ttgagagaa gtgttggat 
        gttttaaga gctgaaggc ttgacttta aacttccaa 
        aagtgtgcc tttctcaaa gaaaccgtt cactttctc 
        ggatttgaa gtaagcgac ggaataata cgccccggg 
        cttgataag attcaggca gtaaaaaat ttctcccca 
        ccgaaaaat gtcaagcag attagacag tttattgga 
        ctaactggg tactttcga catttcgtc aagaattat 
        gcactcatt gctagacca ttaacaaac ttaacacgg 
        aaaggtgtg aattggaaa tgggacacg gaagaggaa 
        cttgcgttt gagaggctt aaagaaatt ttgacgtcg 
        agaccagtg ttatctatt tatgacccc acagctgtc 
        acagagtta cacacagac gcaagttcg ttgggtgtc 
        gctggtatt ttgctacaa tatcagact gacggcaga 
        ctacaccca atagcctat tacagcagg caaacaaac 
        gagcatgag cgacactac cattcgttc gagcttgaa 
        actttggca gtcgttgaa agtgtcaaa aagtttagg 
        atttacttg ctcgacctt gagtttacg atcgttact 
        gactgtaat gagctgaaa gcaacgtcg aacaagtcc 
        caacttatt ccaagaatc gccaggtgg tggctgcaa 
        ctcctagag tttagattt aaagtaaaa tacagagca 
        ggcactcaa atgagtcat gttgacgcc ttaagtcgc 
        aatcccaat gattccaaa acatctaaa gacgtcttg 
        gctttagat aaagcggat tgggtgctt gcaggacag 
        ttgaccgat cctaaaatc aaggaacta cacaatatt 
        ttgtctaaa ccgccatct accgaatac aagcaaaat 
        gttcataag aactatgca ctccgtgaa gaaagggta 
        tatcgaaac acgacgaaa ggattacag tgggtggta 
        ccaaaaggt atgagacag caagtggtt agggccgct 
        catgacgaa cggggacac tttgccgca gaaaaaacg 
        ttaagccga ctctccgat tgttactgg tttcccaga 
        atgcgggac tacgtctcc gattatatt gcatgctgc 
        atcccgtgc ataatgaaa aagaagccg tcagggaaa 
        caagagggt tttcttcac cctatccca aaaccaacc 
        aagcctttc gatacgata catttggac catctgggg 
        ccatttcca agaagcaaa agaggaaac gtgcacatc 
        attgcgtgc attgatgca tgcacaaaa tttcttttt 
        atgcgtccc gtgaagtca acgaagaca gtttttgtc 
        gtcaacttc ctgaacgag ctgtatgtg acctacggg 
        aagccaagg gtggtaatt accgaccag ggaagctgt 
        ttcactgca gccaagttt agggaacac tgtgatcaa 
        aatcaagtc acacatgta aaggtcgcg gtagcaact 
        cccagagct aatggccaa gtagaacga cttaaccga 
        agcatcctg tcggtgctg atgacgggg accttggaa 
        gacaaccta tgggatcaa aatgttcca atggctcaa 
        ttttctatc aataacgcg gcaaatgct tcgaccgga 
        agaacccca agcgaattg atgatgggg tataaacca 
        cgacacggt actgactcg tatttgaga gatgaggtg 
        cagaagatt ccaacgatt gtggacgat cttattaaa 
        ctgcggtta gaagcctct gaaaaggtg gctcaggta 
        cagaaacgt cagaaggaa ctctttgac agaaaaagg 
        aaaccaccc agagagtat aaagtggga gatgtggta 
        gtcgtccgg aaaggggaa gctgcaacg ggtgagagc 
        cggaaactg atggctcca tttagccag cctatgact 
        gtaaaagcg gtattgcca aacgatcgg tacctagtc 
        acggacatg cccaattca cataggacc cgtaggaag 
        gcaatctat cagcgtgtc atcgccgtg gacaggatg 
        aaaccgtcg actaaacct ggaggagtt tcagacgag 
        tccgatggt cctaacgaa gacgatcaa ggtgaacct 
        taaaccgaa gtcgtctcg ggtcgagcc gattatcag 
        gttggccga atgtagcat aattgccta ttttaacaa 
        ttaaatatt ttctataag t
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