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Gene information
General information
TC034131
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006603,TC006602
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11852035..11854482
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034131
    iBeetle screen iB_07551

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034131 RA
      MENGTSDSD STDVESLSD SDEIDEGKN VKNRTKRGI 
      AWCGVEDAP SSHASISAP QISWPPSNP NSNWRQLRP 
      VHFGNPLFG TKMQLYSRT RHNLAVYPD GEVRGTPDD 
      DDLHTYLEV MSAGHPGHV RIKGLLTNL YVAMDPKGR 
      LYGEPDMTD NSTIFIEAF QGSYNTYLS LKYAHLGWY 
      IGIKKSGKF KRGPKTKSE NGFNLTIQD DGHVLGTRD 
      DNLDNVLVV SSSGEISHV RIQGAKSKR YLCFDDSGN 
      LYAEEDKNK DGAIFKEIL QGSYNGYVS KNHPDWYIG 
      IKKDGNMKN GKRTQWGKK SVKFLPNRN VSEAELQQL 
      DSS
    CDS sequence
    • >TC034131 RA
      atggaaaac gggactagt gacagtgat agtaccgat 
      gttgaaagt ttgagtgac agtgatgag atcgatgaa 
      ggaaaaaat gtgaaaaat aggacgaaa aggggaatt 
      gcgtggtgc ggagtggag gacgctccc agttcacac 
      gcctctatt tccgcgccc caaatttcc tggcccccc 
      tccaatccc aactcaaac tggcgccaa ttgcgccct 
      gtccacttc ggaaacccg cttttcggc acaaaaatg 
      cagctctat tccagaact agacacaac ctcgccgtt 
      tatccggac ggcgaagtg cggggaacc cccgacgac 
      gacgacttg cacacttat ttggaggta atgtcggct 
      gggcacccg ggccacgtg cgcattaag ggccttttg 
      acaaacctc tacgtcgcc atggacccc aaagggcgc 
      ttgtacggg gagccagac atgacggac aattctacc 
      atttttatc gaggcgttt caaggctct tacaacacc 
      tatctctcc ctaaagtac gcccatttg gggtggtac 
      atcgggata aaaaaatcg gggaagttc aagcgcggg 
      cccaagacc aagtccgaa aatgggttt aatttgacc 
      atacaagat gatgggcac gttttgggc acaagggat 
      gataattta gacaatgtc ctggttgtt agttcaagt 
      ggggagatt tcccatgtc aggattcaa ggagcgaag 
      agtaaacgg tatctgtgt tttgacgat tcgggaaat 
      ttgtacgcg gaagaagac aaaaataaa gacggggcc 
      atttttaaa gaaatcctt caaggttcg tacaacgga 
      tatgtatca aaaaatcat ccggactgg tacattggg 
      attaaaaaa gatgggaac atgaagaat ggaaaacgg 
      acgcagtgg ggcaaaaaa tcagtgaaa tttttgccc 
      aacagaaat gtatcggaa gcggaattg caacagcta 
      gactccagt tga
    mRNA sequence
    • >TC034131 RA
      tcctgggca caaaaattt aaggtaaaa catcaaaaa 
      tttgtgatt tatgatcgt ctcgtaacc gccgaataa 
      aattctatt tataaccgc ggaaaatta gaaattaga 
      agtaagaac gcgaaaaca gtcgaattt taaacacgt 
      ctgaactaa ttgacggaa attacggtg ttttgcgta 
      caccggctc aatttttga attgttaca aacgtgtgc 
      ccattgttg gttttaatt aacaatcgg caaaaacat 
      ggaaaacgg gactagtga cagtgatag taccgatgt 
      tgaaagttt gagtgacag tgatgagat cgatgaagg 
      aaaaaatgt gaaaaatag gacgaaaag gggaattgc 
      gtggtgcgg agtggagga cgctcccag ttcacacgc 
      ctctatttc cgcgcccca aatttcctg gcccccctc 
      caatcccaa ctcaaactg gcgccaatt gcgccctgt 
      ccacttcgg aaacccgct tttcggcac aaaaatgca 
      gctctattc cagaactag acacaacct cgccgttta 
      tccggacgg cgaagtgcg gggaacccc cgacgacga 
      cgacttgca cacttattt ggaggtaat gtcggctgg 
      gcacccggg ccacgtgcg cattaaggg ccttttgac 
      aaacctcta cgtcgccat ggaccccaa agggcgctt 
      gtacgggga gccagacat gacggacaa ttctaccat 
      ttttatcga ggcgtttca aggctctta caacaccta 
      tctctccct aaagtacgc ccatttggg gtggtacat 
      cgggataaa aaaatcggg gaagttcaa gcgcgggcc 
      caagaccaa gtccgaaaa tgggtttaa tttgaccat 
      acaagatga tgggcacgt tttgggcac aagggatga 
      taatttaga caatgtcct ggttgttag ttcaagtgg 
      ggagatttc ccatgtcag gattcaagg agcgaagag 
      taaacggta tctgtgttt tgacgattc gggaaattt 
      gtacgcgga agaagacaa aaataaaga cggggccat 
      ttttaaaga aatccttca aggttcgta caacggata 
      tgtatcaaa aaatcatcc ggactggta cattgggat 
      taaaaaaga tgggaacat gaagaatgg aaaacggac 
      gcagtgggg caaaaaatc agtgaaatt tttgcccaa 
      cagaaatgt atcggaagc ggaattgca acagctaga 
      ctccagttg atgttttaa ataattgag tatttctac 
      gtaataagt attagcaga tgtttaagc aaaaccttg 
      agcgatagc tccaaaatt agattcgtc tataattgt 
      gagaggaag tcggattgg cataaattt taactagaa 
      tttgaattt cttgtttat tggcggttt tactttgta 
      aaattttat tgtcatgtc aacgctgtc caattatgt 
      tttttttcg aaggctagg aaaaatgct ggatattaa 
      aaaaacaaa tgcagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY