Status
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Gene information
General information
TC005530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11732181..11735539
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005530
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07546

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva segment random irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment randomconstricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005530 RA
      MKDSTPIKT GKKSKKNKH TNEETPETS PNGAKINAF 
      QFMMNRRHR SIGGNSPGR EPDANDSET ASDKSVLKE 
      RKSRFESWA EAKGAAKRK REYEEVGNC ISYKLEKRG 
      KRLKKLLNI DCQSDDEVV TKRKKRKTV VIESDSENE 
      APKTPTANK KWKFKIKLA VDDRSDVEN SSVNSQRNV 
      ENAQGSVEN AQKSVDNAP KSVDNAPKS VENAQGSVE 
      NAQGSVENA QGSVENAQK SVENAQGSV ADVVIVENA 
      PVNTQSSDS GIDVIELDV DPANSVSDE NASRRVLRS 
      RKPKKYDDF LQLSDSDDE KKKKTKVAP VFLKAVPKP 
      KLDPEAMEA RRQFLMSGV PSSLKKEME KVRIFSTEE 
      SDFDIFPSL SHVQQKSDN IFWDLPKVD LKLANVEEK 
      MQFISPLVE NLITRSDGE NKIVNESVQ KITNLKKII 
      KEIKLQNPN YPVYKIFNS LFEKSGQII ETVNDVTPK 
      RRGRKKKKS LEKPPEITS REHTMWTEK YRATSSLDI 
      IGNHKVVSS LKDWLSHWS LSEHKRRGS ESDFEITDS 
      DSRDSATFG NTVILRGPP GSGKTSSIY AICTELGYN 
      ILEVNASSK RTGKRLLQE LQEATQSHQ VKKKQNTSK 
      CVLLVEDID IVFDQDEGF SAALSQIMS TSKRPIVLT 
      MTDEPSAGI QRIINDCRV FQFAPLTPI LTVWLQILC 
      LIEGYFVKI EALAELLAF NKGDIRRTL LQLQLWVKS 
      GGHPDGNHG GSTGGATSV SIDIDDDSN LPDFGTECA 
      SDTIQTHSN CVPIFNWLH NFELLWWNF PRILKMPDF 
      SKHRLNRHI CDFPAGKTE KNKLQALAN CYDSLAFAD 
      VMCQKNRFE ESLERSWRT KEKDSLTLE ENFSLPDYS 
      DFSNDLAQF LLSGQVNNF EDSKVDFDV TLPVPEERR 
      WRSKQIGCE LRLQKAVLP ADCLHRKDV ALDYMPALR 
      SIARSEFER AANSTKRGN RFYSYLKGL GVHGSDVTY 
      KSACDVFKI WD
    CDS sequence
    • >TC005530 RA
      atgaaagat tcaactcca ataaagacc ggtaaaaaa 
      tcaaagaaa aacaagcac accaatgaa gaaaccccc 
      gaaacttcc ccaaatggg gctaaaatc aacgcgttt 
      caatttatg atgaatcga agacatcgg agcattggg 
      gggaattcc cctgggcgt gaacctgac gcaaacgac 
      tcggaaaca gcctcggat aaaagtgtc ttaaaggag 
      aggaaaagt cggtttgag agctgggcg gaggccaag 
      ggggctgcc aaacgtaaa cgagagtac gaggaagtc 
      ggcaattgc atcagttac aagctggag aaacgggga 
      aaacgattg aaaaagttg ctaaatatt gattgtcaa 
      agtgacgat gaggtggtc actaaaagg aagaagcgg 
      aaaactgtt gtaattgaa agtgacagt gagaacgag 
      gcaccaaaa acaccaact gccaacaaa aagtggaaa 
      tttaagatc aagttggcc gtggatgac aggagtgat 
      gttgaaaat tcgtctgtc aacagtcaa cgaaatgtt 
      gaaaatgcc caaggaagt gttgaaaat gcccaaaaa 
      agtgttgac aatgcccca aaaagtgtt gacaatgcc 
      ccaaaaagt gttgaaaat gcccaagga agtgttgaa 
      aatgctcaa ggaagtgtt gaaaatgcc caaggaagt 
      gttgaaaat gctcaaaag agtgttgaa aatgcccaa 
      ggaagtgtt gcagacgtg gtgatagtt gaaaatgcc 
      cctgtcaat acgcagtct agtgacagt ggcattgac 
      gtcatcgaa ctcgacgtg gacccagcc aactctgtt 
      tccgacgaa aatgcctca cgcagagtt ttacgatcc 
      cgaaaaccg aaaaaatac gatgatttt cttcaactt 
      tctgattcg gacgatgag aagaagaag aagacaaaa 
      gtggcacca gttttctta aaagctgtt ccaaaacca 
      aaattagat ccggaagca atggaagca agaagacag 
      tttttgatg agtggtgtt ccgtcttct ttgaaaaaa 
      gagatggaa aaggtgcga atttttagt actgaagaa 
      tcggatttt gatattttc ccgtcgtta agtcacgtc 
      cagcaaaaa tccgacaat attttctgg gatttaccc 
      aaagttgat ttaaagctg gcaaacgtg gaggaaaaa 
      atgcaattt atttctcca cttgtcgaa aatttaata 
      actagaagt gatggtgaa aataaaatt gtcaacgag 
      tctgtgcaa aaaataaca aatttaaaa aaaatcatc 
      aaggagata aaattacaa aatccaaat tatccggtt 
      tataagata tttaattca ttgtttgag aaaagtggt 
      caaataatt gaaaccgtg aatgacgta acaccgaaa 
      cgccgcggt cgcaaaaag aaaaaatct ttggaaaaa 
      ccaccagaa attacatcg cgggaacac acgatgtgg 
      accgagaaa taccgagca acttcctct ctcgacatc 
      atcgggaac cacaaagtt gtgtcatct ttgaaagac 
      tggctttcc cattggtcg ctcagtgaa cacaaaaga 
      cggggcagc gaatcagat ttcgaaatt accgacagc 
      gacagtcgc gacagtgcc acttttggc aacactgtc 
      attttgcgg ggacccccc ggaagcggc aaaacctcg 
      tccatttac gccatttgc accgaactc ggttacaac 
      atacttgaa gttaacgct tcgagcaaa cgcaccggt 
      aaacgcctc cttcaggag ttacaagaa gccacccag 
      tcgcatcaa gtcaagaaa aaacaaaac acgagcaaa 
      tgcgtccta ttggtcgaa gacattgac attgtcttt 
      gaccaagac gagggtttc agtgctgcc ctctcgcag 
      ataatgtca acaagcaag cggccaatt gttttaaca 
      atgaccgac gagccatct gccggcatc caaagaatc 
      ataaacgac tgtcgagtg tttcaattc gcgcctttg 
      acgccaatt ttgacagtt tggttgcaa attttgtgc 
      ctaattgag ggttatttt gtcaaaatc gaggcactt 
      gccgagttg ttggcgttc aataaaggc gacatcagg 
      cggactttg ctgcaatta caactgtgg gtcaagtcg 
      ggtggccac cctgatggc aatcatggt ggtagcact 
      ggtggtgcc actagtgtg tcaattgac attgacgat 
      gattcaaac ttgcccgat tttggcaca gagtgcgct 
      agtgacaca atccagaca cattcgaat tgtgtgcca 
      atttttaat tggttacac aattttgag ttgctttgg 
      tggaacttt ccccgcatt ttaaaaatg cccgatttt 
      tccaaacat cggttaaat cgccacatt tgtgatttc 
      ccggccggg aaaaccgaa aaaaacaaa ttgcaagcg 
      ctggccaat tgttacgat tcgttggcg tttgctgac 
      gtcatgtgt caaaaaaac cggtttgag gaaagtctc 
      gaacggtct tggcggacc aaagaaaag gatagtttg 
      actcttgag gagaatttt tctcttcca gattatagt 
      gattttagc aatgattta gcgcaattt ttattaagt 
      ggccaagtg aataatttt gaggattcg aaggttgat 
      tttgatgtt acactgccg gttccagag gaacgaagg 
      tggcgctcg aaacaaatc gggtgcgag ctgagatta 
      cagaaagct gtattaccg gccgattgt ttacaccgg 
      aaggacgta gctttggat tatatgccg gctttgagg 
      agcatagca cggtccgaa tttgagaga gcggcaaat 
      agtacgaag agggggaac cgcttttat agctacttg 
      aagggttta ggggtgcat ggcagtgac gtcacgtat 
      aagtcggcc tgtgatgtt tttaaaatt tgggattga
    mRNA sequence
    • >TC005530 RA
      ataatgagt tgtgcgggg ttttaccct gatattttg 
      acttaaaac aacccagtt tcgtatcag taaagatca 
      caaatcgcg taatatcaa ttgtggggc ttggtttgt 
      gaattacac ttaatttcg tgttacgta accttagcc 
      acaagaaac ctaacctta aagtctaca aaaaatgtc 
      aacaaagcg ctgatttgg cgccaagtt ttttgtatt 
      gtgttttag tgcagtttt tagtgtgtt atcgaagca 
      aaacatgat ttaggaaca atgaaagat tcaactcca 
      ataaagacc ggtaaaaaa tcaaagaaa aacaagcac 
      accaatgaa gaaaccccc gaaacttcc ccaaatggg 
      gctaaaatc aacgcgttt caatttatg atgaatcga 
      agacatcgg agcattggg gggaattcc cctgggcgt 
      gaacctgac gcaaacgac tcggaaaca gcctcggat 
      aaaagtgtc ttaaaggag aggaaaagt cggtttgag 
      agctgggcg gaggccaag ggggctgcc aaacgtaaa 
      cgagagtac gaggaagtc ggcaattgc atcagttac 
      aagctggag aaacgggga aaacgattg aaaaagttg 
      ctaaatatt gattgtcaa agtgacgat gaggtggtc 
      actaaaagg aagaagcgg aaaactgtt gtaattgaa 
      agtgacagt gagaacgag gcaccaaaa acaccaact 
      gccaacaaa aagtggaaa tttaagatc aagttggcc 
      gtggatgac aggagtgat gttgaaaat tcgtctgtc 
      aacagtcaa cgaaatgtt gaaaatgcc caaggaagt 
      gttgaaaat gcccaaaaa agtgttgac aatgcccca 
      aaaagtgtt gacaatgcc ccaaaaagt gttgaaaat 
      gcccaagga agtgttgaa aatgctcaa ggaagtgtt 
      gaaaatgcc caaggaagt gttgaaaat gctcaaaag 
      agtgttgaa aatgcccaa ggaagtgtt gcagacgtg 
      gtgatagtt gaaaatgcc cctgtcaat acgcagtct 
      agtgacagt ggcattgac gtcatcgaa ctcgacgtg 
      gacccagcc aactctgtt tccgacgaa aatgcctca 
      cgcagagtt ttacgatcc cgaaaaccg aaaaaatac 
      gatgatttt cttcaactt tctgattcg gacgatgag 
      aagaagaag aagacaaaa gtggcacca gttttctta 
      aaagctgtt ccaaaacca aaattagat ccggaagca 
      atggaagca agaagacag tttttgatg agtggtgtt 
      ccgtcttct ttgaaaaaa gagatggaa aaggtgcga 
      atttttagt actgaagaa tcggatttt gatattttc 
      ccgtcgtta agtcacgtc cagcaaaaa tccgacaat 
      attttctgg gatttaccc aaagttgat ttaaagctg 
      gcaaacgtg gaggaaaaa atgcaattt atttctcca 
      cttgtcgaa aatttaata actagaagt gatggtgaa 
      aataaaatt gtcaacgag tctgtgcaa aaaataaca 
      aatttaaaa aaaatcatc aaggagata aaattacaa 
      aatccaaat tatccggtt tataagata tttaattca 
      ttgtttgag aaaagtggt caaataatt gaaaccgtg 
      aatgacgta acaccgaaa cgccgcggt cgcaaaaag 
      aaaaaatct ttggaaaaa ccaccagaa attacatcg 
      cgggaacac acgatgtgg accgagaaa taccgagca 
      acttcctct ctcgacatc atcgggaac cacaaagtt 
      gtgtcatct ttgaaagac tggctttcc cattggtcg 
      ctcagtgaa cacaaaaga cggggcagc gaatcagat 
      ttcgaaatt accgacagc gacagtcgc gacagtgcc 
      acttttggc aacactgtc attttgcgg ggacccccc 
      ggaagcggc aaaacctcg tccatttac gccatttgc 
      accgaactc ggttacaac atacttgaa gttaacgct 
      tcgagcaaa cgcaccggt aaacgcctc cttcaggag 
      ttacaagaa gccacccag tcgcatcaa gtcaagaaa 
      aaacaaaac acgagcaaa tgcgtccta ttggtcgaa 
      gacattgac attgtcttt gaccaagac gagggtttc 
      agtgctgcc ctctcgcag ataatgtca acaagcaag 
      cggccaatt gttttaaca atgaccgac gagccatct 
      gccggcatc caaagaatc ataaacgac tgtcgagtg 
      tttcaattc gcgcctttg acgccaatt ttgacagtt 
      tggttgcaa attttgtgc ctaattgag ggttatttt 
      gtcaaaatc gaggcactt gccgagttg ttggcgttc 
      aataaaggc gacatcagg cggactttg ctgcaatta 
      caactgtgg gtcaagtcg ggtggccac cctgatggc 
      aatcatggt ggtagcact ggtggtgcc actagtgtg 
      tcaattgac attgacgat gattcaaac ttgcccgat 
      tttggcaca gagtgcgct agtgacaca atccagaca 
      cattcgaat tgtgtgcca atttttaat tggttacac 
      aattttgag ttgctttgg tggaacttt ccccgcatt 
      ttaaaaatg cccgatttt tccaaacat cggttaaat 
      cgccacatt tgtgatttc ccggccggg aaaaccgaa 
      aaaaacaaa ttgcaagcg ctggccaat tgttacgat 
      tcgttggcg tttgctgac gtcatgtgt caaaaaaac 
      cggtttgag gaaagtctc gaacggtct tggcggacc 
      aaagaaaag gatagtttg actcttgag gagaatttt 
      tctcttcca gattatagt gattttagc aatgattta 
      gcgcaattt ttattaagt ggccaagtg aataatttt 
      gaggattcg aaggttgat tttgatgtt acactgccg 
      gttccagag gaacgaagg tggcgctcg aaacaaatc 
      gggtgcgag ctgagatta cagaaagct gtattaccg 
      gccgattgt ttacaccgg aaggacgta gctttggat 
      tatatgccg gctttgagg agcatagca cggtccgaa 
      tttgagaga gcggcaaat agtacgaag agggggaac 
      cgcttttat agctacttg aagggttta ggggtgcat 
      ggcagtgac gtcacgtat aagtcggcc tgtgatgtt 
      tttaaaatt tgggattga tttttgtat attgaataa 
      aacgcacga gttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY