Status
Please wait ...
Gene information
General information
TC005538 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11603446..11606389
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005538
    iBeetle screen iB_07542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    vitellogenic egg chamber potentially latefused - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005538 RA
      MVKESSSLK KKRKSGTSA DNEAEIDIY KKSVEFEEK 
      EALNRPNDT DAQIYFNTC KEIRHLFAE ISELKMKSG 
      SDVRAKITE KTTEVCLLL VLLKKLNRM EKVRLVFPK 
      EQLAAEKQK VDSINLQYQ NLLYEANHL MSEYRKCFQ 
      FKSKDEHIE LVPVEEFLA EAPKNLTTK FKKFDETDE 
      MAKHELRLA RLEWELQQR IHLADMCKK LEEEKKKLE 
      GDIAERRNK LDKLGPLLS SVMEATKPV QEHLGLHID 
      KIQAEHKLA SLLPNPLYL FYANIDAYR QVNDSGMSV 
      EIMGDQEEA SQWKELELA EDEEEEEED MEPEAEIQE 
      VEEIVETKK RRHRKSVHV DPLEEKKRK LLQVHPLSV 
      QVTVTTKDG PSIKIRLSY YAKLEIVTV SSSVNIPSN 
      ITGNAAREV LSNESVLVE LVEGDFGLD SPNPCTPYQ 
      LKKVGLGSF QSLVPELGY VYTWAQKMC GIDFLNKMS 
      GSDQLSQTN VESVMKTIK KRLKSRYAL AKQLEELER 
      NVIPVLPAS VDLPRTTIS SLTKWSSGT YQSFCEAKF 
      TEGLLEAEL ISPNDIFYK ATITRDKAN LQAFVVIKN 
      DYPSIPPIF ALCLNYNGT RHSQNDDNI RDMERSINV 
      DWNHEFSNA NWLLSAQIM SLCVGLDIY LETEDPGTF 
      QQNTMYIKS TCARNRRKP FKFRNIGVG VYTQ
    CDS sequence
    • >TC005538 RA
      atggttaaa gagtcaagt agtttgaaa aaaaagcgc 
      aaaagtggg actagtgcc gacaatgag gccgaaatc 
      gacatttat aaaaaatcg gtcgaattt gaggagaaa 
      gaggccctc aatcgaccg aacgatacc gacgctcag 
      atttacttc aacacgtgc aaagaaatt cggcattta 
      ttcgccgaa ataagtgaa ctgaaaatg aagagtggg 
      tctgatgtc agggctaaa attacggaa aaaaccacc 
      gaagtgtgt ctattattg gtgcttttg aaaaaactg 
      aaccgcatg gagaaggtg cgtttggtt tttccaaaa 
      gaacaattg gctgcggaa aaacaaaaa gtggacagt 
      attaattta caatatcag aacttgttg tacgaggct 
      aaccatctg atgtcggaa tatcgcaaa tgtttccag 
      ttcaagtct aaagacgag cacatcgag ctggtcccc 
      gttgaggag ttcctcgcc gaagcgccg aaaaattta 
      acgacaaaa ttcaaaaaa tttgatgaa accgacgaa 
      atggcgaag cacgagctg cggctcgcg cgcctcgag 
      tgggagctc cagcagcgt attcatttg gccgacatg 
      tgcaaaaaa cttgaagag gagaagaaa aaactagag 
      ggggatatt gccgaacgt cgcaataaa ttggataaa 
      ttggggccc ctcttgtct tcggtcatg gaggccacg 
      aaaccggtg caggagcac ctggggctc cacattgac 
      aagatccag gctgaacac aagcttgcc tcgcttcta 
      cccaacccc ctttacttg ttttatgcc aatattgac 
      gcctacaga caggtgaac gactcgggg atgagtgtg 
      gagattatg ggggaccag gaggaggct agtcaatgg 
      aaggagttg gagttggca gaggatgaa gaagaggag 
      gaggaggac atggaaccg gaagctgag atacaggag 
      gtggaggaa attgtcgaa acgaagaaa cggcgtcac 
      cggaagtcg gtacatgtt gatccactt gaggagaaa 
      aaacgcaag ttgttacaa gttcacccg ctttctgta 
      caagttact gtcactact aaagacggt ccttcaatt 
      aaaattcga ctaagttat tacgcaaaa ttggaaatc 
      gtcactgta tcttcaagc gtcaatatt ccgagcaat 
      attacagga aacgcagct cgtgaggtc ttatcaaac 
      gaaagcgtc ctcgtggag ctggtggag ggcgacttc 
      gggctggac agccccaac ccgtgcacc ccttaccaa 
      ctgaaaaaa gtcggcttg gggtccttc caaagcctc 
      gtcccagag cttggctac gtctacacg tgggcgcaa 
      aaaatgtgc ggcattgat tttttgaat aaaatgtcc 
      ggttcggat caactgagc caaactaac gtcgagtcg 
      gtcatgaaa acaataaaa aagcgccta aagtcgcgg 
      tatgccttg gcgaaacaa ttggaggag ttggagcgg 
      aatgtgatt cctgttttg ccggctagt gtcgatttg 
      ccgcgaacc acgatttcg agtcttacg aaatggtca 
      tcagggacg tatcaaagc ttttgcgag gctaaattt 
      acggagggt ttgctagaa gctgagtta atttcgccg 
      aatgatatt ttctataaa gcgacaatc acgcgtgat 
      aaagcaaat ttgcaagct tttgttgtt atcaagaac 
      gattatccg tcaattcca ccaattttt gcgctttgt 
      ttgaactat aacgggact agacattcg cagaatgat 
      gataatatt cgggacatg gaacggtcg attaatgtc 
      gattggaac cacgaattt tcaaatgca aattggctt 
      ttgtcggcc cagattatg tcattgtgt gtcggactg 
      gacatttat ctggaaacg gaggaccct gggacgttt 
      caacagaac accatgtat attaaatcg acttgcgcc 
      agaaatagg agaaagccg ttcaaattc aggaatatt 
      ggggttggg gtctacacc caatga
    mRNA sequence
    • >TC005538 RA
      cgtaacaac taataattg aaattatcc aaattattt 
      ataatataa gtcgcaatg tttggattt tgatttaaa 
      aacacagaa attaattcg tttaaacca agatgtggt 
      gtcctgaaa caaaatggc gtctgttgt ctgtcagtt 
      tgcgcattt ctaacctca aattttttg tcggcttgt 
      ttacataat cttttttgc ttaaaaaaa tggttaaag 
      agtcaagta gtttgaaaa aaaagcgca aaagtggga 
      ctagtgccg acaatgagg ccgaaatcg acatttata 
      aaaaatcgg tcgaatttg aggagaaag aggccctca 
      atcgaccga acgataccg acgctcaga tttacttca 
      acacgtgca aagaaattc ggcatttat tcgccgaaa 
      taagtgaac tgaaaatga agagtgggt ctgatgtca 
      gggctaaaa ttacggaaa aaaccaccg aagtgtgtc 
      tattattgg tgcttttga aaaaactga accgcatgg 
      agaaggtgc gtttggttt ttccaaaag aacaattgg 
      ctgcggaaa aacaaaaag tggacagta ttaatttac 
      aatatcaga acttgttgt acgaggcta accatctga 
      tgtcggaat atcgcaaat gtttccagt tcaagtcta 
      aagacgagc acatcgagc tggtccccg ttgaggagt 
      tcctcgccg aagcgccga aaaatttaa cgacaaaat 
      tcaaaaaat ttgatgaaa ccgacgaaa tggcgaagc 
      acgagctgc ggctcgcgc gcctcgagt gggagctcc 
      agcagcgta ttcatttgg ccgacatgt gcaaaaaac 
      ttgaagagg agaagaaaa aactagagg gggatattg 
      ccgaacgtc gcaataaat tggataaat tggggcccc 
      tcttgtctt cggtcatgg aggccacga aaccggtgc 
      aggagcacc tggggctcc acattgaca agatccagg 
      ctgaacaca agcttgcct cgcttctac ccaaccccc 
      tttacttgt tttatgcca atattgacg cctacagac 
      aggtgaacg actcgggga tgagtgtgg agattatgg 
      gggaccagg aggaggcta gtcaatgga aggagttgg 
      agttggcag aggatgaag aagaggagg aggaggaca 
      tggaaccgg aagctgaga tacaggagg tggaggaaa 
      ttgtcgaaa cgaagaaac ggcgtcacc ggaagtcgg 
      tacatgttg atccacttg aggagaaaa aacgcaagt 
      tgttacaag ttcacccgc tttctgtac aagttactg 
      tcactacta aagacggtc cttcaatta aaattcgac 
      taagttatt acgcaaaat tggaaatcg tcactgtat 
      cttcaagcg tcaatattc cgagcaata ttacaggaa 
      acgcagctc gtgaggtct tatcaaacg aaagcgtcc 
      tcgtggagc tggtggagg gcgacttcg ggctggaca 
      gccccaacc cgtgcaccc cttaccaac tgaaaaaag 
      tcggcttgg ggtccttcc aaagcctcg tcccagagc 
      ttggctacg tctacacgt gggcgcaaa aaatgtgcg 
      gcattgatt ttttgaata aaatgtccg gttcggatc 
      aactgagcc aaactaacg tcgagtcgg tcatgaaaa 
      caataaaaa agcgcctaa agtcgcggt atgccttgg 
      cgaaacaat tggaggagt tggagcgga atgtgattc 
      ctgttttgc cggctagtg tcgatttgc cgcgaacca 
      cgatttcga gtcttacga aatggtcat cagggacgt 
      atcaaagct tttgcgagg ctaaattta cggagggtt 
      tgctagaag ctgagttaa tttcgccga atgatattt 
      tctataaag cgacaatca cgcgtgata aagcaaatt 
      tgcaagctt ttgttgtta tcaagaacg attatccgt 
      caattccac caatttttg cgctttgtt tgaactata 
      acgggacta gacattcgc agaatgatg ataatattc 
      gggacatgg aacggtcga ttaatgtcg attggaacc 
      acgaatttt caaatgcaa attggcttt tgtcggccc 
      agattatgt cattgtgtg tcggactgg acatttatc 
      tggaaacgg aggaccctg ggacgtttc aacagaaca 
      ccatgtata ttaaatcga cttgcgcca gaaatagga 
      gaaagccgt tcaaattca ggaatattg gggttgggg 
      tctacaccc aatgagttt ttttttttg acaaataaa 
      tgagttttt tgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY