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Gene information
General information
TC004616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6559007..6564136
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004616
    iBeetle screen iB_07532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004616 RA
      MFTGLTNQV SSWMGSAKG EQEEKVPTP TEEGDVVLN 
      QEKKDASPT KPTGGSKLD MLTNVKSQI EGIGGWLGS 
      SIPKLRKGE EGAEEQPSA EEGNTPASA ESTKGSPQL 
      KDDDDNSSA TGGADSGPQ SIAETPTEE KEGQFGNVQ 
      SKALAGAKS FGSFLYSAV NKAGKTVSE ASAKIKETV 
      EKNSILGEF NKEQEAFMK GQGGNTATA LPPWVGCSN 
      EEALKEECL SLSTDKKNF LRSPPAGID FQFDYEVSY 
      PVAMAIMEK DPNLEKMRY ELVPKVISE ENFWRNYFY 
      RVSLICQAN ELSSMSRDG DSQSQPLTS DQPIASSDD 
      EMEEFVSDS LQASSEDFV EVAERMMKT VIVPQPKEE 
      WERELVAEL HDYEVVAEQ EPSSKRDIW EQEIEEIEE 
      MLKDGTDLK
    • >TC004616 RB
      MFTGLTNQV SSWMGSAKG EQEEKVPTP TEEGDVVLN 
      QEKKDASPT KPTGGSKLD MLTNVKSQI EGIGGWLGS 
      SIPKLRKGE EGAEEQPSA EEGNTPASA ESTKGSPQL 
      KDDDDNSSA TGGADSGPQ SIAETPTEE KEGQFGNVQ 
      SKALAGAKS FGSFLYSAV NKAGKTVSE ASAKIKETV 
      EKNSILGEF NKEQEAFMK GQGGNTATA LPPWVGCSN 
      EEALKEECL SLSTDKKNF LRSPPAGID FQFDYEVSY 
      PVAMAIMEK DPNLEKMRY ELVPKVISE ENFWRNYFY 
      RVSLICQAN ELSSMSRDG DSQSQPLTS DQPIASSDD 
      EMEEFVSDS LQASSEDFV EVAERMMKT VIVPQPKGK 
      TCCFCCVCL TSRFKLMSK LWLVSCLIL SQLKKNGVN 
      CSFHYPLSC VSLFCSFDP DLSRFIFMS
    CDS sequence
    • >TC004616 RA
      atgttcacg gggctcact aaccaggtc agctcttgg 
      atgggctct gccaagggg gaacaggag gaaaaagtg 
      cccactccg accgaagaa ggcgacgtg gtgctcaac 
      caagagaag aaagatgca agccccacg aagcccacc 
      gggggcagc aaactggac atgttgacc aacgtcaag 
      tcgcagatc gagggcatt gggggctgg ctgggctcc 
      agcatcccc aagctgagg aaaggcgaa gaaggcgcc 
      gaggagcag ccctcggct gaggagggc aacacgcca 
      gcctcggcg gagtccacc aagggctcg ccccaactc 
      aaagacgac gacgacaac tccagtgcc accggaggc 
      gccgactct ggaccacaa tcaattgct gaaacacca 
      actgaagaa aaggagggt cagttcggg aacgtgcaa 
      agcaaggct ctggctggg gcgaagtcc ttcggctcg 
      ttcctgtac tcagctgta aataaggcc ggcaagacc 
      gtgagcgag gcgagtgcg aagataaag gaaacggta 
      gaaaaaaat agtatcctt ggggaattt aataaggaa 
      caggaggcg ttcatgaag ggccagggc ggcaacacc 
      gccactgcc cttccgccc tgggttggt tgttccaac 
      gaggaggcc ctcaaagaa gagtgcctc agtctttca 
      actgacaaa aaaaatttc cttcgtagt cccccggct 
      ggaatcgac ttccagttc gactatgag gtgtcttac 
      cccgtcgct atggctata atggaaaag gaccctaat 
      ctggagaag atgcgatat gaacttgtg cctaaagta 
      ataagtgaa gagaatttt tggaggaat tacttctac 
      cgtgttagt ctaatttgt caggcgaac gaactgtcg 
      tccatgtct cgtgatggc gacagtcag tcgcaacct 
      ctcacttct gatcaacct atagctagt tcagacgac 
      gagatggag gagtttgtg tcagatagt ttgcaggct 
      agcagcgaa gattttgtt gaagtggcc gaacgcatg 
      atgaaaact gtcattgtg ccgcagcca aaggaagaa 
      tgggagcgg gagttggtg gcagaactg cacgactat 
      gaagtagtc gccgaacaa gagcccagc agcaagcgg 
      gacatctgg gagcaggag atcgaggaa atcgaagaa 
      atgctgaag gatggcacg gatttaaaa taa
    • >TC004616 RB
      atgttcacg gggctcact aaccaggtc agctcttgg 
      atgggctct gccaagggg gaacaggag gaaaaagtg 
      cccactccg accgaagaa ggcgacgtg gtgctcaac 
      caagagaag aaagatgca agccccacg aagcccacc 
      gggggcagc aaactggac atgttgacc aacgtcaag 
      tcgcagatc gagggcatt gggggctgg ctgggctcc 
      agcatcccc aagctgagg aaaggcgaa gaaggcgcc 
      gaggagcag ccctcggct gaggagggc aacacgcca 
      gcctcggcg gagtccacc aagggctcg ccccaactc 
      aaagacgac gacgacaac tccagtgcc accggaggc 
      gccgactct ggaccacaa tcaattgct gaaacacca 
      actgaagaa aaggagggt cagttcggg aacgtgcaa 
      agcaaggct ctggctggg gcgaagtcc ttcggctcg 
      ttcctgtac tcagctgta aataaggcc ggcaagacc 
      gtgagcgag gcgagtgcg aagataaag gaaacggta 
      gaaaaaaat agtatcctt ggggaattt aataaggaa 
      caggaggcg ttcatgaag ggccagggc ggcaacacc 
      gccactgcc cttccgccc tgggttggt tgttccaac 
      gaggaggcc ctcaaagaa gagtgcctc agtctttca 
      actgacaaa aaaaatttc cttcgtagt cccccggct 
      ggaatcgac ttccagttc gactatgag gtgtcttac 
      cccgtcgct atggctata atggaaaag gaccctaat 
      ctggagaag atgcgatat gaacttgtg cctaaagta 
      ataagtgaa gagaatttt tggaggaat tacttctac 
      cgtgttagt ctaatttgt caggcgaac gaactgtcg 
      tccatgtct cgtgatggc gacagtcag tcgcaacct 
      ctcacttct gatcaacct atagctagt tcagacgac 
      gagatggag gagtttgtg tcagatagt ttgcaggct 
      agcagcgaa gattttgtt gaagtggcc gaacgcatg 
      atgaaaact gtcattgtg ccgcagcca aagggtaag 
      acgtgttgc ttttgctgt gtctgtcta acgtctaga 
      tttaaacta atgtcaaag ctgtggtta gtttcctgt 
      ttgattttg tcgcagctt aagaagaac ggcgtcaac 
      tgtagcttc cattatcca ctgtcctgt gtctcctta 
      ttttgctcc tttgatcca gacttatcc aggtttatt 
      tttatgagt tga
    mRNA sequence
    • >TC004616 RA
      cgcaagcga tggttgccg atagaaata gcgaagtga 
      aaagtgagc aagaatagc aaatacaca gcgtagagc 
      aaaatcggg ccttaaaac tctccaacc cgttcccga 
      ccgaaagta tgtgtttgt ttgcgcttt aatcagttc 
      gggaaagac tccagtgtc ctttgttcg ctctctccg 
      accagtggc accgaagag gcaaaatgt tcacggggc 
      tcactaacc aggtcagct cttggatgg gctctgcca 
      agggggaac aggaggaaa aagtgccca ctccgaccg 
      aagaaggcg acgtggtgc tcaaccaag agaagaaag 
      atgcaagcc ccacgaagc ccaccgggg gcagcaaac 
      tggacatgt tgaccaacg tcaagtcgc agatcgagg 
      gcattgggg gctggctgg gctccagca tccccaagc 
      tgaggaaag gcgaagaag gcgccgagg agcagccct 
      cggctgagg agggcaaca cgccagcct cggcggagt 
      ccaccaagg gctcgcccc aactcaaag acgacgacg 
      acaactcca gtgccaccg gaggcgccg actctggac 
      cacaatcaa ttgctgaaa caccaactg aagaaaagg 
      agggtcagt tcgggaacg tgcaaagca aggctctgg 
      ctggggcga agtccttcg gctcgttcc tgtactcag 
      ctgtaaata aggccggca agaccgtga gcgaggcga 
      gtgcgaaga taaaggaaa cggtagaaa aaaatagta 
      tccttgggg aatttaata aggaacagg aggcgttca 
      tgaagggcc agggcggca acaccgcca ctgcccttc 
      cgccctggg ttggttgtt ccaacgagg aggccctca 
      aagaagagt gcctcagtc tttcaactg acaaaaaaa 
      atttccttc gtagtcccc cggctggaa tcgacttcc 
      agttcgact atgaggtgt cttaccccg tcgctatgg 
      ctataatgg aaaaggacc ctaatctgg agaagatgc 
      gatatgaac ttgtgccta aagtaataa gtgaagaga 
      atttttgga ggaattact tctaccgtg ttagtctaa 
      tttgtcagg cgaacgaac tgtcgtcca tgtctcgtg 
      atggcgaca gtcagtcgc aacctctca cttctgatc 
      aacctatag ctagttcag acgacgaga tggaggagt 
      ttgtgtcag atagtttgc aggctagca gcgaagatt 
      ttgttgaag tggccgaac gcatgatga aaactgtca 
      ttgtgccgc agccaaagg aagaatggg agcgggagt 
      tggtggcag aactgcacg actatgaag tagtcgccg 
      aacaagagc ccagcagca agcgggaca tctgggagc 
      aggagatcg aggaaatcg aagaaatgc tgaaggatg 
      gcacggatt taaaataag cgcgctatt ccctgttat 
      tttgggctt ctattcata tcggaccga ctgatttac 
      attccgttg aacttattt gtctagtca ttacatcag 
      agttgggca acagaatta ttcgcgagg ctcggcttt 
      gactataag tgtattatt ttatgtaac tttgtgata 
      tttactctt ggggtctac acctgaaat atcttacaa 
      gatacaata actattatc acattaata aatgtaata 
      aaattgtgt gcatttgct atggaggct gtttcaaat 
      cattccttt tagtcttag gtacttgtg taaataatc 
      attcaacac tctctaact gtagctgtt gttattttc 
      aacgctcgt gtcacgttc tcacaaatg atccgcgtt 
      tttggaagt cattttcgc ggtacaacg gtttgtaag 
      agcgtgacg tggcatcaa atctatata ttctaatga 
      aatgttttt tgttaatga tcttttgct gctttagaa 
      gcgaattta aaaaattac taatcttga gttagtgtt 
      gcaaacggt ttatatttt tttattaaa ctgttcagt 
      tcgtttcaa atgttaatg tcatagtac taaaatgta 
      gcttacgtg tattaggca tatttatcc tgtattcta 
      attgttata catcataaa aattgttaa ataaatgta 
      gcaactgtg cccagtatg atggaattc ttcattgct 
      tagtccacc tcagagtaa aatgaaaat gtatatttc 
      aagtcgata ttgtaatca gattatata acccagcaa 
      gatgcaaca gatgtgaat actgtatag acaagtact 
      tataataaa tcaataata atgttggat ttgttaatg 
      cacccatta gagttctgc aaactgagt tgtgatgca 
      ttcgtttca aaatttatt gtttaaaca ataaataat 
      tacaaggaa a
    • >TC004616 RB
      cgcaagcga tggttgccg atagaaata gcgaagtga 
      aaagtgagc aagaatagc aaatacaca gcgtagagc 
      aaaatcggg ccttaaaac tctccaacc cgttcccga 
      ccgaaagta tgtgtttgt ttgcgcttt aatcagttc 
      gggaaagac tccagtgtc ctttgttcg ctctctccg 
      accagtggc accgaagag gcaaaatgt tcacggggc 
      tcactaacc aggtcagct cttggatgg gctctgcca 
      agggggaac aggaggaaa aagtgccca ctccgaccg 
      aagaaggcg acgtggtgc tcaaccaag agaagaaag 
      atgcaagcc ccacgaagc ccaccgggg gcagcaaac 
      tggacatgt tgaccaacg tcaagtcgc agatcgagg 
      gcattgggg gctggctgg gctccagca tccccaagc 
      tgaggaaag gcgaagaag gcgccgagg agcagccct 
      cggctgagg agggcaaca cgccagcct cggcggagt 
      ccaccaagg gctcgcccc aactcaaag acgacgacg 
      acaactcca gtgccaccg gaggcgccg actctggac 
      cacaatcaa ttgctgaaa caccaactg aagaaaagg 
      agggtcagt tcgggaacg tgcaaagca aggctctgg 
      ctggggcga agtccttcg gctcgttcc tgtactcag 
      ctgtaaata aggccggca agaccgtga gcgaggcga 
      gtgcgaaga taaaggaaa cggtagaaa aaaatagta 
      tccttgggg aatttaata aggaacagg aggcgttca 
      tgaagggcc agggcggca acaccgcca ctgcccttc 
      cgccctggg ttggttgtt ccaacgagg aggccctca 
      aagaagagt gcctcagtc tttcaactg acaaaaaaa 
      atttccttc gtagtcccc cggctggaa tcgacttcc 
      agttcgact atgaggtgt cttaccccg tcgctatgg 
      ctataatgg aaaaggacc ctaatctgg agaagatgc 
      gatatgaac ttgtgccta aagtaataa gtgaagaga 
      atttttgga ggaattact tctaccgtg ttagtctaa 
      tttgtcagg cgaacgaac tgtcgtcca tgtctcgtg 
      atggcgaca gtcagtcgc aacctctca cttctgatc 
      aacctatag ctagttcag acgacgaga tggaggagt 
      ttgtgtcag atagtttgc aggctagca gcgaagatt 
      ttgttgaag tggccgaac gcatgatga aaactgtca 
      ttgtgccgc agccaaagg gtaagacgt gttgctttt 
      gctgtgtct gtctaacgt ctagattta aactaatgt 
      caaagctgt ggttagttt cctgtttga ttttgtcgc 
      agcttaaga agaacggcg tcaactgta gcttccatt 
      atccactgt cctgtgtct ccttatttt gctcctttg 
      atccagact tatccaggt ttattttta tgagttgac 
      tttaatgta tgttttgtt gaacacatg ttacttaac 
      gtgttaaaa gtagttcct tattgacac tgtacattt 
      tgaaatggt ctaaattga atctgtttg taccgcgtg 
      atcataatt tggtccttc ggttgttcg tgatcaaca 
      aaaatcgcg tcccaaacg tcaaattac tccactcaa 
      agaccgact tttgttaat cactgcgaa ctgagctac 
      agttttctg gtcaaccgg tgttaacta tttaggtgg 
      gcatatgtg caattaatt ttagcatat ttgcgcgca 
      atttgtgga acgtgattc ttcaaaaaa caaaaaacc 
      aaaaaaatg atacgttgg ctggactgt gtcacttga 
      gtaattttg ttgacaatt ttaaatctc ttgtaaatg 
      tggtgtatt ttggcaatg taattgtag gttttaata 
      tatgtgtct tcctaaaat tacggtcaa ggacacagt 
      ttgggttgc ttactaaag acaatactg ggaaggtca 
      gatgtaaaa tatacgaag tgttttaac taaaatact 
      gtaagagga ccgacagga cacacttac tgtttaaag 
      aggttcaaa atttaacgg tgtggaccc accagttgc 
      tcagacttg acactcgag aaacaggaa actaacact 
      ctgagcatc tggtttatt gctcaaact gtaacactg 
      ttttctttt gtgttttta tttcgtgcc tatccttat 
      ccccctgtt tttcacata gaagaatgg gagcgggag 
      ttggtggca gaactgcac gactatgaa gtagtcgcc 
      gaacaagag cccagcagc aagcgggac atctgggag 
      caggagatc gaggaaatc gaagaaatg ctgaaggat 
      ggcacggat ttaaaataa gcgcgctat tccctgtta 
      ttttgggct tctattcat atcggaccg actgattta 
      cattccgtt gaacttatt tgtctagtc attacatca 
      gagttgggc aacagaatt attcgcgag gctcggctt 
      tgactataa gtgtattat tttatgtaa ctttgtgat 
      atttactct tggggtcta cacctgaaa tatcttaca 
      agatacaat aactattat cacattaat aaatgtaat 
      aaaattgtg tgcatttgc tatggaggc tgtttcaaa 
      tcattcctt ttagtctta ggtacttgt gtaaataat 
      cattcaaca ctctctaac tgtagctgt tgttatttt 
      caacgctcg tgtcacgtt ctcacaaat gatccgcgt 
      ttttggaag tcattttcg cggtacaac ggtttgtaa 
      gagcgtgac gtggcatca aatctatat attctaatg 
      aaatgtttt ttgttaatg atcttttgc tgctttaga 
      agcgaattt aaaaaatta ctaatcttg agttagtgt 
      tgcaaacgg tttatattt ttttattaa actgttcag 
      ttcgtttca aatgttaat gtcatagta ctaaaatgt 
      agcttacgt gtattaggc atatttatc ctgtattct 
      aattgttat acatcataa aaattgtta aataaatgt 
      agcaactgt gcccagtat gatggaatt cttcattgc 
      ttagtccac ctcagagta aaatgaaaa tgtatattt 
      caagtcgat attgtaatc agattatat aacccagca 
      agatgcaac agatgtgaa tactgtata gacaagtac 
      ttataataa atcaataat aatgttgga tttgttaat 
      gcacccatt agagttctg caaactgag ttgtgatgc 
      attcgtttc aaaatttat tgtttaaac aataaataa 
      ttacaagga aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY