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Gene information
General information
TC005581 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11054835..11056701
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005581
    iBeetle screen iB_07531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005581 RA
      MAKLEYLTG ATFTLKCAV LYPIDSNNP KIKKILYAV 
WAIFFILTF VTGFIQCFV FVCINPFDL VQEAMIIMS 
LVFYSTTFF YFIVFYKNW QNMVALVTN INKNFHRAT 
DNVIEKISM DQASELSDK LAYVWTSSL AVGSVVPVV 
LAIATGNLE MPMPAWFPY DYNKSPVFE ITYLWQVFC 
LITLAIIYG ASDMFFPCI TIIIGQQFK ILASNFKNN 
FYTSLIKLG AEESIVQNF SKDIKTHEF RSFYIKYGN 
IFKILNNAK FQTLNRAFL KRNIKHHKL LLRFCEDLN 
KILNTFLLI RVSAIVFNL IFIGFNIII STDRTLMLG 
FCNYFCFGS TELFIHTYS GQILTENAD FLWTLYECP 
WYLCDVTYQ KMLILVQMR VKRMVSTKA GNFFTMIAP 
SFIAFQRAV FSYITLLKE VTDLGKD
    CDS sequence
    • >TC005581 RA
      atggcaaaa ctggagtac ctcacgggg gcgactttc 
acccttaaa tgcgcggtt ttgtaccca attgactcc 
aacaatccg aaaataaaa aaaatcttg tacgcagtt 
tgggcgatt tttttcatc ctaactttc gtaaccggc 
tttattcaa tgttttgtt ttcgtttgc attaatccg 
ttcgatttg gtgcaagag gctatgatt ataatgagt 
ttagttttt tactcaacc acgtttttt tattttata 
gtgttttac aaaaattgg caaaatatg gtcgcactt 
gtaacaaat atcaataaa aattttcac agggctaca 
gataatgtt attgaaaaa atttcaatg gaccaggcg 
agcgaatta tccgataaa ctggcatat gtgtggact 
tcaagcctg gctgttggt tcagtggtc cctgtagtt 
ttagccata gcgactgga aatttagaa atgccaatg 
ccggcctgg tttccctac gattacaac aaatctcca 
gtttttgaa ataacatac ctgtggcaa gttttctgt 
ctaataacc ttggcaatt atttacggg gcctccgat 
atgtttttc ccttgcata acaataata atcggacaa 
cagttcaaa atcctggcc agcaatttc aaaaataac 
ttctacaca tcgctcata aaattaggg gccgaggaa 
tcaatcgtc caaaacttc agcaaagac attaaaaca 
cacgagttt cggtcattc tacataaaa tacggtaat 
atttttaaa attctaaac aacgccaaa tttcagact 
cttaatcgg gcgtttctg aaacgaaac ataaaacac 
cacaaactc ctgctgaga ttttgcgaa gacttgaat 
aaaatattg aacacattt ttactaatc cgagtttcg 
gcgatcgtt ttcaatttg attttcatc ggttttaac 
atcataatt tcaaccgac aggacccta atgttgggt 
ttttgcaat tacttttgt ttcggaagc accgagctg 
tttatacac acgtacagt gggcaaatt ttgactgag 
aacgctgat tttttgtgg actttgtac gaatgtccg 
tggtatttg tgcgacgtt acgtatcaa aaaatgttg 
attcttgtt caaatgaga gtgaaaagg atggtttcc 
accaaagct gggaatttt ttcacgatg attgcaccg 
tctttcatt gcctttcaa agagccgtg ttttcgtac 
attactctg ctgaaggaa gtgactgat ttggggaaa 
gattaa
    mRNA sequence
    • >TC005581 RA
      cttgtcacc gttgaccca ctttaaact tgtcactat 
ttaaagctc catttgccg ccaacacgt catttgtgg 
cgacaatgg caaaactgg agtacctca cgggggcga 
ctttcaccc ttaaatgcg cggttttgt acccaattg 
actccaaca atccgaaaa taaaaaaaa tcttgtacg 
cagtttggg cgatttttt tcatcctaa ctttcgtaa 
ccggcttta ttcaatgtt ttgttttcg tttgcatta 
atccgttcg atttggtgc aagaggcta tgattataa 
tgagtttag ttttttact caaccacgt ttttttatt 
ttatagtgt tttacaaaa attggcaaa atatggtcg 
cacttgtaa caaatatca ataaaaatt ttcacaggg 
ctacagata atgttattg aaaaaattt caatggacc 
aggcgagcg aattatccg ataaactgg catatgtgt 
ggacttcaa gcctggctg ttggttcag tggtccctg 
tagttttag ccatagcga ctggaaatt tagaaatgc 
caatgccgg cctggtttc cctacgatt acaacaaat 
ctccagttt ttgaaataa catacctgt ggcaagttt 
tctgtctaa taaccttgg caattattt acggggcct 
ccgatatgt ttttccctt gcataacaa taataatcg 
gacaacagt tcaaaatcc tggccagca atttcaaaa 
ataacttct acacatcgc tcataaaat taggggccg 
aggaatcaa tcgtccaaa acttcagca aagacatta 
aaacacacg agtttcggt cattctaca taaaatacg 
gtaatattt ttaaaattc taaacaacg ccaaatttc 
agactctta atcgggcgt ttctgaaac gaaacataa 
aacaccaca aactcctgc tgagatttt gcgaagact 
tgaataaaa tattgaaca catttttac taatccgag 
tttcggcga tcgttttca atttgattt tcatcggtt 
ttaacatca taatttcaa ccgacagga ccctaatgt 
tgggttttt gcaattact tttgtttcg gaagcaccg 
agctgttta tacacacgt acagtgggc aaattttga 
ctgagaacg ctgattttt tgtggactt tgtacgaat 
gtccgtggt atttgtgcg acgttacgt atcaaaaaa 
tgttgattc ttgttcaaa tgagagtga aaaggatgg 
tttccacca aagctggga attttttca cgatgattg 
caccgtctt tcattgcct ttcaaagag ccgtgtttt 
cgtacatta ctctgctga aggaagtga ctgatttgg 
ggaaagatt aatcattaa aaaagcacc acaaagtga 
gaacagaaa tgtaatttg tgatagctt tagcaaatc 
tgaatggca ctatcagag aattcaatt agagctcac 
cgattcatt ttttaacac aaaatttgt taaacattt 
aattgcaaa caaaattta attcgcatt caggaaagt 
gacttcaag ctttatgtt caatatagg caagcaaca 
atgcggact acaaataca gactggttc atgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY