Status
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Gene information
General information
TC005581 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11054835..11056701
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005581
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005581 RA
      MAKLEYLTG ATFTLKCAV LYPIDSNNP KIKKILYAV 
      WAIFFILTF VTGFIQCFV FVCINPFDL VQEAMIIMS 
      LVFYSTTFF YFIVFYKNW QNMVALVTN INKNFHRAT 
      DNVIEKISM DQASELSDK LAYVWTSSL AVGSVVPVV 
      LAIATGNLE MPMPAWFPY DYNKSPVFE ITYLWQVFC 
      LITLAIIYG ASDMFFPCI TIIIGQQFK ILASNFKNN 
      FYTSLIKLG AEESIVQNF SKDIKTHEF RSFYIKYGN 
      IFKILNNAK FQTLNRAFL KRNIKHHKL LLRFCEDLN 
      KILNTFLLI RVSAIVFNL IFIGFNIII STDRTLMLG 
      FCNYFCFGS TELFIHTYS GQILTENAD FLWTLYECP 
      WYLCDVTYQ KMLILVQMR VKRMVSTKA GNFFTMIAP 
      SFIAFQRAV FSYITLLKE VTDLGKD
    CDS sequence
    • >TC005581 RA
      atggcaaaa ctggagtac ctcacgggg gcgactttc 
      acccttaaa tgcgcggtt ttgtaccca attgactcc 
      aacaatccg aaaataaaa aaaatcttg tacgcagtt 
      tgggcgatt tttttcatc ctaactttc gtaaccggc 
      tttattcaa tgttttgtt ttcgtttgc attaatccg 
      ttcgatttg gtgcaagag gctatgatt ataatgagt 
      ttagttttt tactcaacc acgtttttt tattttata 
      gtgttttac aaaaattgg caaaatatg gtcgcactt 
      gtaacaaat atcaataaa aattttcac agggctaca 
      gataatgtt attgaaaaa atttcaatg gaccaggcg 
      agcgaatta tccgataaa ctggcatat gtgtggact 
      tcaagcctg gctgttggt tcagtggtc cctgtagtt 
      ttagccata gcgactgga aatttagaa atgccaatg 
      ccggcctgg tttccctac gattacaac aaatctcca 
      gtttttgaa ataacatac ctgtggcaa gttttctgt 
      ctaataacc ttggcaatt atttacggg gcctccgat 
      atgtttttc ccttgcata acaataata atcggacaa 
      cagttcaaa atcctggcc agcaatttc aaaaataac 
      ttctacaca tcgctcata aaattaggg gccgaggaa 
      tcaatcgtc caaaacttc agcaaagac attaaaaca 
      cacgagttt cggtcattc tacataaaa tacggtaat 
      atttttaaa attctaaac aacgccaaa tttcagact 
      cttaatcgg gcgtttctg aaacgaaac ataaaacac 
      cacaaactc ctgctgaga ttttgcgaa gacttgaat 
      aaaatattg aacacattt ttactaatc cgagtttcg 
      gcgatcgtt ttcaatttg attttcatc ggttttaac 
      atcataatt tcaaccgac aggacccta atgttgggt 
      ttttgcaat tacttttgt ttcggaagc accgagctg 
      tttatacac acgtacagt gggcaaatt ttgactgag 
      aacgctgat tttttgtgg actttgtac gaatgtccg 
      tggtatttg tgcgacgtt acgtatcaa aaaatgttg 
      attcttgtt caaatgaga gtgaaaagg atggtttcc 
      accaaagct gggaatttt ttcacgatg attgcaccg 
      tctttcatt gcctttcaa agagccgtg ttttcgtac 
      attactctg ctgaaggaa gtgactgat ttggggaaa 
      gattaa
    mRNA sequence
    • >TC005581 RA
      cttgtcacc gttgaccca ctttaaact tgtcactat 
      ttaaagctc catttgccg ccaacacgt catttgtgg 
      cgacaatgg caaaactgg agtacctca cgggggcga 
      ctttcaccc ttaaatgcg cggttttgt acccaattg 
      actccaaca atccgaaaa taaaaaaaa tcttgtacg 
      cagtttggg cgatttttt tcatcctaa ctttcgtaa 
      ccggcttta ttcaatgtt ttgttttcg tttgcatta 
      atccgttcg atttggtgc aagaggcta tgattataa 
      tgagtttag ttttttact caaccacgt ttttttatt 
      ttatagtgt tttacaaaa attggcaaa atatggtcg 
      cacttgtaa caaatatca ataaaaatt ttcacaggg 
      ctacagata atgttattg aaaaaattt caatggacc 
      aggcgagcg aattatccg ataaactgg catatgtgt 
      ggacttcaa gcctggctg ttggttcag tggtccctg 
      tagttttag ccatagcga ctggaaatt tagaaatgc 
      caatgccgg cctggtttc cctacgatt acaacaaat 
      ctccagttt ttgaaataa catacctgt ggcaagttt 
      tctgtctaa taaccttgg caattattt acggggcct 
      ccgatatgt ttttccctt gcataacaa taataatcg 
      gacaacagt tcaaaatcc tggccagca atttcaaaa 
      ataacttct acacatcgc tcataaaat taggggccg 
      aggaatcaa tcgtccaaa acttcagca aagacatta 
      aaacacacg agtttcggt cattctaca taaaatacg 
      gtaatattt ttaaaattc taaacaacg ccaaatttc 
      agactctta atcgggcgt ttctgaaac gaaacataa 
      aacaccaca aactcctgc tgagatttt gcgaagact 
      tgaataaaa tattgaaca catttttac taatccgag 
      tttcggcga tcgttttca atttgattt tcatcggtt 
      ttaacatca taatttcaa ccgacagga ccctaatgt 
      tgggttttt gcaattact tttgtttcg gaagcaccg 
      agctgttta tacacacgt acagtgggc aaattttga 
      ctgagaacg ctgattttt tgtggactt tgtacgaat 
      gtccgtggt atttgtgcg acgttacgt atcaaaaaa 
      tgttgattc ttgttcaaa tgagagtga aaaggatgg 
      tttccacca aagctggga attttttca cgatgattg 
      caccgtctt tcattgcct ttcaaagag ccgtgtttt 
      cgtacatta ctctgctga aggaagtga ctgatttgg 
      ggaaagatt aatcattaa aaaagcacc acaaagtga 
      gaacagaaa tgtaatttg tgatagctt tagcaaatc 
      tgaatggca ctatcagag aattcaatt agagctcac 
      cgattcatt ttttaacac aaaatttgt taaacattt 
      aattgcaaa caaaattta attcgcatt caggaaagt 
      gacttcaag ctttatgtt caatatagg caagcaaca 
      atgcggact acaaataca gactggttc atgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY