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Gene information
General information
TC006499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10622270..10625004
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006499
    iBeetle screen iB_07528

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006499 RA
      MSFSITNKL FPSNHKTVF VLDHTPYFG ISCESPIEL 
      EFLKARTPG FIPMTPICK SLWTSSVES AIEYCRIVW 
      DLFPQGKLV RFIASDTVA HILNTWSPS QQNLSHVMN 
      AMSLIGVPP PHAINRNLE YTVLHGLRA AIEALSDCT 
      DIQVKKSQN QTFGDVQTK LINRCRVIC ITSARDNDS 
      MKRLEEIFL SVLQQQNKV AAGSDRLLT IDHCHLVII 
      NTFPVNIES QVNNHPPKN LSTILTTEV HSIKAPLIS 
      NKLSTLILE HYNLASTTV TGIPMKEEQ NASSSANYD 
      VEIYHASSA HTAILKGNA LDSAAIRTI KDGLEYETV 
      TLKWCTPRG CSGSELQNC TGMFRITPV DVNSRPSLC 
      LINFLLNGR SVMLEMPRK AGGKITSHL LASHGGEIF 
      IHTLCTARS VLEDPPSIS EGCGGRVTD YRITDFGLL 
      IKQNTLVPI KAGFVDEGN RPIQKMKTR LNRHTKYWP 
      LTISSTLIF NLKGYIDPL PSIITKEKI TEEEVYQCR 
      RIIFNLISL ESKNESLYS LNAGQRVKG QKREDQYKA 
      MWNELETLL KKNLHTADH RSVYNCLLE CHKFNFEEE 
      KLSDKVELD EALRELDHI GPSKSDSDA PRASVIRAT 
      TDSPMSPPP LTSINPPVA KSGTSRSGG IYSAPRTLL 
      DIFTSQEKV KSRPEFSGR LAGGAVAKL YANHKYADS 
      GSRGESMEV E
    CDS sequence
    • >TC006499 RA
      atgtcattt tcaataaca aataagttg tttccgtcg 
      aaccacaag acggttttt gtgctagac cacacgccc 
      tatttcggt atatcatgc gaaagcccc atagagctc 
      gagtttttg aaagccagg actcccggc tttatcccc 
      atgaccccc atttgcaag tcgctgtgg acctccagc 
      gttgagtcg gctatcgag tattgcagg atagtctgg 
      gacctgttc ccgcaaggg aaattggtt aggttcatt 
      gctagcgac actgttgct catattttg aatacatgg 
      agcccctcg cagcagaat ctgagccac gttatgaac 
      gcgatgtcg ctaattggg gttccgccc cctcatgcc 
      atcaatcgc aacttggaa tataccgtt ttgcacggc 
      ttgagggcc gcgattgaa gccctcagt gactgcacg 
      gacatccaa gtgaaaaag agccaaaat caaactttt 
      ggcgacgtg caaacgaaa ctaataaat cggtgcaga 
      gtgatttgc atcacttcg gcacgtgac aatgacagt 
      atgaagcga ttggaggaa atttttttg agtgtcttg 
      caacaacaa aacaaagtt gccgcagga tctgacaga 
      ttattgaca attgaccat tgccacctt gtcataatc 
      aacactttc ccggtcaat atcgagtca caagtcaat 
      aatcacccc ccaaaaaat ttgtcaaca attttgaca 
      acggaggtt cactcaatc aaagccccc ttgatctcg 
      aacaaactc tccactctt attttggag cactataac 
      ttggctagt accacagtt acggggatt cccatgaaa 
      gaggagcaa aatgcgagt tcctcggcc aactacgac 
      gtggagatt taccacgcg agctccgct cacaccgcc 
      atcctcaag ggcaatgcc ctggattcg gccgccatt 
      cgcaccatc aaagacggc ctggagtac gaaacggtc 
      accttaaag tggtgtacg ccgcgtggg tgttccggg 
      tccgagctc caaaattgc accggaatg ttccggatc 
      actccggtc gatgtcaat tccaggccg tccctttgc 
      cttatcaat ttcctcctg aacgggagg tcagtcatg 
      ctggaaatg ccgcggaag gcgggaggg aaaatcacg 
      agtcatttg ttggcctcc catgggggc gaaattttc 
      atccacact ttgtgcacg gcaaggtcg gtcctggag 
      gaccccccg agcatttcc gaagggtgt ggcgggaga 
      gttaccgac tataggatc actgacttt gggttgttg 
      atcaaacag aacaccttg gtgccgatc aaggccggg 
      tttgtggac gaggggaat aggccgatt cagaagatg 
      aagacgagg ctgaatagg cacacgaag tattggccc 
      ttgacgatt agctcaact ttgattttt aacttgaaa 
      gggtacatt gatcctctt ccgtcgatt ataacgaaa 
      gaaaaaatc acggaagag gaagtgtac cagtgcaga 
      cgcattatt ttcaatctg atatcgctt gagagcaaa 
      aacgagtcg ttgtactcg ttgaacgcg ggtcaaagg 
      gtgaagggc caaaaaagg gaggaccag tataaggcc 
      atgtggaac gaactggag acactcctt aagaaaaat 
      ctgcacaca gcagaccac aggagcgtt tacaactgt 
      cttttagag tgtcacaaa ttcaatttc gaggaggaa 
      aaactttca gataaagtc gaactagat gaagcttta 
      cgcgaattg gatcacata ggaccgtcg aaatccgac 
      tctgacgcc ccccgagct tcggttatc cgcgccact 
      acagactcg ccaatgtcg cccccacca ctcaccagt 
      attaaccct cccgtggcc aaatcgggc actagcagg 
      tctgggggc atttattca gccccccga actctgctc 
      gatatcttc acatcgcag gagaaggtg aagtcacgg 
      ccggagttc agcgggagg ctggcaggt ggcgctgtt 
      gcaaaactg tacgccaat cacaagtat gccgattcg 
      gggtctaga ggggagagt atggaagtt gaataa
    mRNA sequence
    • >TC006499 RA
      attgtcaag catttaaac gcgcgccaa ttttttgac 
      aagtcacgt gacaaaaca actcaaata acctcacaa 
      aaagagtac aaaaaatgt cattttcaa taacaaata 
      agttgtttc cgtcgaacc acaagacgg tttttgtgc 
      tagaccaca cgccctatt tcggtatat catgcgaaa 
      gccccatag agctcgagt ttttgaaag ccaggactc 
      ccggcttta tccccatga cccccattt gcaagtcgc 
      tgtggacct ccagcgttg agtcggcta tcgagtatt 
      gcaggatag tctgggacc tgttcccgc aagggaaat 
      tggttaggt tcattgcta gcgacactg ttgctcata 
      ttttgaata catggagcc cctcgcagc agaatctga 
      gccacgtta tgaacgcga tgtcgctaa ttggggttc 
      cgccccctc atgccatca atcgcaact tggaatata 
      ccgttttgc acggcttga gggccgcga ttgaagccc 
      tcagtgact gcacggaca tccaagtga aaaagagcc 
      aaaatcaaa cttttggcg acgtgcaaa cgaaactaa 
      taaatcggt gcagagtga tttgcatca cttcggcac 
      gtgacaatg acagtatga agcgattgg aggaaattt 
      ttttgagtg tcttgcaac aacaaaaca aagttgccg 
      caggatctg acagattat tgacaattg accattgcc 
      accttgtca taatcaaca ctttcccgg tcaatatcg 
      agtcacaag tcaataatc accccccaa aaaatttgt 
      caacaattt tgacaacgg aggttcact caatcaaag 
      cccccttga tctcgaaca aactctcca ctcttattt 
      tggagcact ataacttgg ctagtacca cagttacgg 
      ggattccca tgaaagagg agcaaaatg cgagttcct 
      cggccaact acgacgtgg agatttacc acgcgagct 
      ccgctcaca ccgccatcc tcaagggca atgccctgg 
      attcggccg ccattcgca ccatcaaag acggcctgg 
      agtacgaaa cggtcacct taaagtggt gtacgccgc 
      gtgggtgtt ccgggtccg agctccaaa attgcaccg 
      gaatgttcc ggatcactc cggtcgatg tcaattcca 
      ggccgtccc tttgcctta tcaatttcc tcctgaacg 
      ggaggtcag tcatgctgg aaatgccgc ggaaggcgg 
      gagggaaaa tcacgagtc atttgttgg cctcccatg 
      ggggcgaaa ttttcatcc acactttgt gcacggcaa 
      ggtcggtcc tggaggacc ccccgagca tttccgaag 
      ggtgtggcg ggagagtta ccgactata ggatcactg 
      actttgggt tgttgatca aacagaaca ccttggtgc 
      cgatcaagg ccgggtttg tggacgagg ggaataggc 
      cgattcaga agatgaaga cgaggctga ataggcaca 
      cgaagtatt ggcccttga cgattagct caactttga 
      tttttaact tgaaagggt acattgatc ctcttccgt 
      cgattataa cgaaagaaa aaatcacgg aagaggaag 
      tgtaccagt gcagacgca ttattttca atctgatat 
      cgcttgaga gcaaaaacg agtcgttgt actcgttga 
      acgcgggtc aaagggtga agggccaaa aaagggagg 
      accagtata aggccatgt ggaacgaac tggagacac 
      tccttaaga aaaatctgc acacagcag accacagga 
      gcgtttaca actgtcttt tagagtgtc acaaattca 
      atttcgagg aggaaaaac tttcagata aagtcgaac 
      tagatgaag ctttacgcg aattggatc acataggac 
      cgtcgaaat ccgactctg acgcccccc gagcttcgg 
      ttatccgcg ccactacag actcgccaa tgtcgcccc 
      caccactca ccagtatta accctcccg tggccaaat 
      cgggcacta gcaggtctg ggggcattt attcagccc 
      cccgaactc tgctcgata tcttcacat cgcaggaga 
      aggtgaagt cacggccgg agttcagcg ggaggctgg 
      caggtggcg ctgttgcaa aactgtacg ccaatcaca 
      agtatgccg attcggggt ctagagggg agagtatgg 
      aagttgaat aaaaaaatg ccgtgaaag atttatatt 
      tatgaaaat atcctacaa aaaacctca aactcattt 
      tctacaact gatattttt acagttaca ttgttcaga 
      ttatgaata tctcctgtt tgctatgat tacaagaag 
      cggcgtgaa taggagcga ataaaaaac atgaaaaaa 
      gtcggttca agacattca taatccact ttaaaaaat 
      ataacacat tactatata tataatttt aaataaatt 
      cactaacta tctgttttt tgtaaaagt gttatgtat 
      acattctac agtattatt cataaaagg cattcagtt 
      atttgttgt atctata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY