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Gene information
General information
TC034105 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10459431..10463138
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034105
    iBeetle screen iB_07524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034105 RA
      MSFRLAILL LPLAVAFGE DICQTCSCK AKANAQIYT 
      INCANKSLD HTLTTWPPQ NNTIIANFA GNNLRTLEP 
      LPPTNASVE IILSRCNIK YLQAALFAA APNVKFVDL 
      SYNQLPAEE IASEKFRGP INNTEYRPI AVENLNLAY 
      NQIHSLGMN VFEHMPNLK ILNLEGNDF TMLDIHTQL 
      ALTSVRQLQ SLNLANNEL TEMIAGAIQ THKNLTELN 
      LSNNLLDFV PSSLAQIGQ SLQILNLDN NPIIELTSE 
      SFLGLGNII ELSANNLTK LKYVNANTF ASMPKLKKL 
      SLSNNFHLR DIDREAFAA NQTLEEFYL NNNELFKLD 
      FKLLPWSKL HIFEFKNNP FDCSCDLYN ITLALSKDI 
      IRSEDGPYC MDPRTDREM QIFYLKSDI CSVKSKHVT 
      RTESIEYQF SVVRISLIV LSVILMMIA LVAAVLGVI 
      KYRRYQRNM SYPYATQIA CGYSDNGQT LTLDCSKKN 
      FIHMLAAWP PHNATIKAS FAYNYLGHL QKLPPTEAV 
      VEIILSNCN LKVLEPGPF DTTVNIKFL DLSHNLLTS 
      EQLSAGVFK GPYNGAKPT EIALEELDL SYNQIHSLH 
      NNVFKFAPH LKRLNLKGN DFVLLDIQT QLALTSLRS 
      LSSLNLADN ELTDLVEGA IEGLDYLRE LDLSINYLD 
      FVPHSLKAV AKSLQVVNL DGNPIIEMT EESFQGLEK 
      LIELSANNL TKLHQINSS AFAPALSLK KLSLAHNPS 
      LSDINVNAF SPNQSIEEL YLNNNNLST LPFDLIPWH 
      HLTSFALSD NPLTCSCDL YKIATSLPS SITRTSNHP 
      TCFDLYTST TFMVYNLDS VICDPQTLP AIKPRILHH 
      HFSIPLTGL IVVLSLGLF VAIALGYSR YKKFASATR 
      DPYVSQVVY SPLFNRAV
    CDS sequence
    • >TC034105 RA
      atgtctttt cgtttggcc atcctcctg ctacccctc 
      gcggtggct tttggtgaa gacatctgt caaacctgt 
      agttgcaaa gcaaaagcc aacgcccag atctacaca 
      ataaactgt gccaacaaa agcctagac cacactcta 
      acaacctgg ccgccccaa aacaacaca ataatcgca 
      aacttcgcc ggcaacaac ctaaggaca cttgagccg 
      cttccacca acaaatgcc agtgttgag ataatcttg 
      tcacggtgt aacatcaaa tacctacaa gccgcacta 
      tttgccgct gcgccaaac gtcaaattc gttgatctt 
      agttacaat caattgcca gccgaagaa atagcctcg 
      gaaaagttt aggggaccg atcaacaac accgagtat 
      cggccaatc gccgtcgaa aacctaaac ctggcctac 
      aatcagatc cacagcctg ggcatgaac gttttcgaa 
      catatgccc aacttgaaa atacttaat ttggaaggt 
      aacgacttt accatgttg gatatacac acacaactg 
      gcattgacg tcggttagg caactgcag agtctcaac 
      ctggccaat aacgaatta actgaaatg atcgccggt 
      gccattcag actcacaaa aacttaacc gagctgaat 
      ttatcgaac aatttgctt gatttcgtc ccgtctagt 
      ttagcccag attggtcaa agtctgcaa atactcaac 
      ctggacaat aacccgatc attgaattg acatctgag 
      tcgtttcta ggcctcgga aatataatc gaactgtcg 
      gctaacaat ttaacaaaa ctgaaatac gtcaatgct 
      aacactttt gcctccatg ccgaaacta aaaaaactg 
      agtctctca aacaatttc catttgagg gacatagac 
      cgcgaggct ttcgccgct aaccaaacc ctcgaagag 
      ttctatttg aacaacaac gagctcttc aaactcgac 
      tttaagcta ctgccatgg tcaaaatta cacattttt 
      gaattcaaa aacaacccg tttgattgt tcctgtgac 
      ttgtacaac atcaccctg gcgctgtcc aaagacatt 
      atccgatca gaggacggc ccttattgc atggaccct 
      agaacggac cgcgaaatg caaattttt tacttgaaa 
      agtgatatc tgctcagtc aagagcaaa catgtgact 
      cggactgag tccatcgag taccagttt tctgtcgtt 
      aggataagt ttgattgtc ttgagtgta attttgatg 
      atgatagcg ttagttgct gcggttttg ggtgtgata 
      aagtacagg aggtaccag aggaatatg agctatccg 
      tacgctacg cagattgct tgtggatac tccgataac 
      ggccaaacc ctcactctc gactgttct aagaaaaat 
      ttcattcat atgttagcc gcgtggcct ccacacaac 
      gccactatt aaagcaagt ttcgcttat aattacctc 
      ggccacttg caaaaactc ccaccaact gaggctgtt 
      gttgaaatc atactttca aactgcaat ctcaaagtt 
      ttagaaccg ggacctttt gataccacc gtcaatata 
      aaatttctt gacttaagt cataatctg ctaacgtcg 
      gaacaactt agcgccggt gtttttaag ggtccctac 
      aatggggcc aaaccgact gagatagct ctcgaagag 
      ctagatttg agctataat cagattcac agcctgcat 
      aataacgtt tttaaattc gcgccgcat ctgaaacgg 
      cttaatctc aaagggaat gactttgtc ttgttggat 
      atacagaca caattggcc cttacttct ttaagaagt 
      ctttcgagt ctgaatttg gccgataat gagctgacc 
      gatttggtg gaaggtgca attgaagga ctggactat 
      ttgcgcgag ttggatttg tcaatcaat tacttggat 
      tttgtgccg catagtttg aaagcggtc gccaaaagt 
      ctgcaagtg gtgaattta gacggaaat cctattatc 
      gaaatgacc gaggaaagc tttcaaggc ttagaaaaa 
      ctgatcgaa ttatctgcc aacaacttg accaaactt 
      caccagatc aattcgagt gctttcgca cccgcccta 
      agcttgaaa aaactaagc cttgcacac aacccttca 
      ctctcagac atcaacgtc aacgctttc tcccccaac 
      caatcaatc gaagagctg tacttaaac aacaacaat 
      ctttcaacc cttcctttc gacctcatc ccttggcac 
      cacttgacc agcttcgct ctcagcgac aacccccta 
      acttgtagc tgcgacttg tacaaaatc gccacttcc 
      cttccaagt tccataacc cgaacttca aaccatccc 
      acctgtttc gacctttac acctcgacc accttcatg 
      gtctacaac ctcgacagc gtcatctgc gacccccaa 
      accctccca gctatcaaa cctcgaata ttacaccac 
      cacttctcc atccccctc accggcctc atcgtggtc 
      ctgtcactg gggctcttc gtagccatc gccttgggc 
      tactcccga tataaaaaa ttcgcttcg gcgaccaga 
      gacccatac gtatcgcag gtcgtctat agtccgctg 
      ttcaatcgc gcggtctaa
    mRNA sequence
    • >TC034105 RA
      tcaagtaca aaatgtctt ttcgtttgg ccatcctcc 
      tgctacccc tcgcggtgg cttttggtg aagacatct 
      gtcaaacct gtagttgca aagcaaaag ccaacgccc 
      agatctaca caataaact gtgccaaca aaagcctag 
      accacactc taacaacct ggccgcccc aaaacaaca 
      caataatcg caaacttcg ccggcaaca acctaagga 
      cacttgagc cgcttccac caacaaatg ccagtgttg 
      agataatct tgtcacggt gtaacatca aatacctac 
      aagccgcac tatttgccg ctgcgccaa acgtcaaat 
      tcgttgatc ttagttaca atcaattgc cagccgaag 
      aaatagcct cggaaaagt ttaggggac cgatcaaca 
      acaccgagt atcggccaa tcgccgtcg aaaacctaa 
      acctggcct acaatcaga tccacagcc tgggcatga 
      acgttttcg aacatatgc ccaacttga aaatactta 
      atttggaag gtaacgact ttaccatgt tggatatac 
      acacacaac tggcattga cgtcggtta ggcaactgc 
      agagtctca acctggcca ataacgaat taactgaaa 
      tgatcgccg gtgccattc agactcaca aaaacttaa 
      ccgagctga atttatcga acaatttgc ttgatttcg 
      tcccgtcta gtttagccc agattggtc aaagtctgc 
      aaatactca acctggaca ataacccga tcattgaat 
      tgacatctg agtcgtttc taggcctcg gaaatataa 
      tcgaactgt cggctaaca atttaacaa aactgaaat 
      acgtcaatg ctaacactt ttgcctcca tgccgaaac 
      taaaaaaac tgagtctct caaacaatt tccatttga 
      gggacatag accgcgagg ctttcgccg ctaaccaaa 
      ccctcgaag agttctatt tgaacaaca acgagctct 
      tcaaactcg actttaagc tactgccat ggtcaaaat 
      tacacattt ttgaattca aaaacaacc cgtttgatt 
      gttcctgtg acttgtaca acatcaccc tggcgctgt 
      ccaaagaca ttatccgat cagaggacg gcccttatt 
      gcatggacc ctagaacgg accgcgaaa tgcaaattt 
      tttacttga aaagtgata tctgctcag tcaagagca 
      aacatgtga ctcggactg agtccatcg agtaccagt 
      tttctgtcg ttaggataa gtttgattg tcttgagtg 
      taattttga tgatgatag cgttagttg ctgcggttt 
      tgggtgtga taaagtaca ggaggtacc agaggaata 
      tgagctatc cgtacgcta cgcagattg cttgtggat 
      actccgata acggccaaa ccctcactc tcgactgtt 
      ctaagaaaa atttcattc atatgttag ccgcgtggc 
      ctccacaca acgccacta ttaaagcaa gtttcgctt 
      ataattacc tcggccact tgcaaaaac tcccaccaa 
      ctgaggctg ttgttgaaa tcatacttt caaactgca 
      atctcaaag ttttagaac cgggacctt ttgatacca 
      ccgtcaata taaaatttc ttgacttaa gtcataatc 
      tgctaacgt cggaacaac ttagcgccg gtgttttta 
      agggtccct acaatgggg ccaaaccga ctgagatag 
      ctctcgaag agctagatt tgagctata atcagattc 
      acagcctgc ataataacg tttttaaat tcgcgccgc 
      atctgaaac ggcttaatc tcaaaggga atgactttg 
      tcttgttgg atatacaga cacaattgg cccttactt 
      ctttaagaa gtctttcga gtctgaatt tggccgata 
      atgagctga ccgatttgg tggaaggtg caattgaag 
      gactggact atttgcgcg agttggatt tgtcaatca 
      attacttgg attttgtgc cgcatagtt tgaaagcgg 
      tcgccaaaa gtctgcaag tggtgaatt tagacggaa 
      atcctatta tcgaaatga ccgaggaaa gctttcaag 
      gcttagaaa aactgatcg aattatctg ccaacaact 
      tgaccaaac ttcaccaga tcaattcga gtgctttcg 
      cacccgccc taagcttga aaaaactaa gccttgcac 
      acaaccctt cactctcag acatcaacg tcaacgctt 
      tctccccca accaatcaa tcgaagagc tgtacttaa 
      acaacaaca atctttcaa cccttcctt tcgacctca 
      tcccttggc accacttga ccagcttcg ctctcagcg 
      acaaccccc taacttgta gctgcgact tgtacaaaa 
      tcgccactt cccttccaa gttccataa cccgaactt 
      caaaccatc ccacctgtt tcgaccttt acacctcga 
      ccaccttca tggtctaca acctcgaca gcgtcatct 
      gcgaccccc aaaccctcc cagctatca aacctcgaa 
      tattacacc accacttct ccatccccc tcaccggcc 
      tcatcgtgg tcctgtcac tggggctct tcgtagcca 
      tcgccttgg gctactccc gatataaaa aattcgctt 
      cggcgacca gagacccat acgtatcgc aggtcgtct 
      atagtccgc tgttcaatc gcgcggtct aaaataaaa 
      tctcacctc tgctgttcc ggccagctg cttcgtata 
      cgttaggaa ttggttgag gaggtcttc ggctttccg 
      ggcgacctc tttgaacct gtagagagc ttcgagctg 
      ttcgatgca tctcacaca gatgacgtg cggcagagg 
      gtcgctctt ggcaacctg gaaagtgat cattgcggt 
      gattaatgc gtgttgcgt aaattttcg t
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