| General information | |||
| TC034105 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 10459431..10463138 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034105 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07524 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034105 RA
MSFRLAILL LPLAVAFGE DICQTCSCK AKANAQIYT INCANKSLD HTLTTWPPQ NNTIIANFA GNNLRTLEP LPPTNASVE IILSRCNIK YLQAALFAA APNVKFVDL SYNQLPAEE IASEKFRGP INNTEYRPI AVENLNLAY NQIHSLGMN VFEHMPNLK ILNLEGNDF TMLDIHTQL ALTSVRQLQ SLNLANNEL TEMIAGAIQ THKNLTELN LSNNLLDFV PSSLAQIGQ SLQILNLDN NPIIELTSE SFLGLGNII ELSANNLTK LKYVNANTF ASMPKLKKL SLSNNFHLR DIDREAFAA NQTLEEFYL NNNELFKLD FKLLPWSKL HIFEFKNNP FDCSCDLYN ITLALSKDI IRSEDGPYC MDPRTDREM QIFYLKSDI CSVKSKHVT RTESIEYQF SVVRISLIV LSVILMMIA LVAAVLGVI KYRRYQRNM SYPYATQIA CGYSDNGQT LTLDCSKKN FIHMLAAWP PHNATIKAS FAYNYLGHL QKLPPTEAV VEIILSNCN LKVLEPGPF DTTVNIKFL DLSHNLLTS EQLSAGVFK GPYNGAKPT EIALEELDL SYNQIHSLH NNVFKFAPH LKRLNLKGN DFVLLDIQT QLALTSLRS LSSLNLADN ELTDLVEGA IEGLDYLRE LDLSINYLD FVPHSLKAV AKSLQVVNL DGNPIIEMT EESFQGLEK LIELSANNL TKLHQINSS AFAPALSLK KLSLAHNPS LSDINVNAF SPNQSIEEL YLNNNNLST LPFDLIPWH HLTSFALSD NPLTCSCDL YKIATSLPS SITRTSNHP TCFDLYTST TFMVYNLDS VICDPQTLP AIKPRILHH HFSIPLTGL IVVLSLGLF VAIALGYSR YKKFASATR DPYVSQVVY SPLFNRAV
- >TC034105 RA
atgtctttt cgtttggcc atcctcctg ctacccctc gcggtggct tttggtgaa gacatctgt caaacctgt agttgcaaa gcaaaagcc aacgcccag atctacaca ataaactgt gccaacaaa agcctagac cacactcta acaacctgg ccgccccaa aacaacaca ataatcgca aacttcgcc ggcaacaac ctaaggaca cttgagccg cttccacca acaaatgcc agtgttgag ataatcttg tcacggtgt aacatcaaa tacctacaa gccgcacta tttgccgct gcgccaaac gtcaaattc gttgatctt agttacaat caattgcca gccgaagaa atagcctcg gaaaagttt aggggaccg atcaacaac accgagtat cggccaatc gccgtcgaa aacctaaac ctggcctac aatcagatc cacagcctg ggcatgaac gttttcgaa catatgccc aacttgaaa atacttaat ttggaaggt aacgacttt accatgttg gatatacac acacaactg gcattgacg tcggttagg caactgcag agtctcaac ctggccaat aacgaatta actgaaatg atcgccggt gccattcag actcacaaa aacttaacc gagctgaat ttatcgaac aatttgctt gatttcgtc ccgtctagt ttagcccag attggtcaa agtctgcaa atactcaac ctggacaat aacccgatc attgaattg acatctgag tcgtttcta ggcctcgga aatataatc gaactgtcg gctaacaat ttaacaaaa ctgaaatac gtcaatgct aacactttt gcctccatg ccgaaacta aaaaaactg agtctctca aacaatttc catttgagg gacatagac cgcgaggct ttcgccgct aaccaaacc ctcgaagag ttctatttg aacaacaac gagctcttc aaactcgac tttaagcta ctgccatgg tcaaaatta cacattttt gaattcaaa aacaacccg tttgattgt tcctgtgac ttgtacaac atcaccctg gcgctgtcc aaagacatt atccgatca gaggacggc ccttattgc atggaccct agaacggac cgcgaaatg caaattttt tacttgaaa agtgatatc tgctcagtc aagagcaaa catgtgact cggactgag tccatcgag taccagttt tctgtcgtt aggataagt ttgattgtc ttgagtgta attttgatg atgatagcg ttagttgct gcggttttg ggtgtgata aagtacagg aggtaccag aggaatatg agctatccg tacgctacg cagattgct tgtggatac tccgataac ggccaaacc ctcactctc gactgttct aagaaaaat ttcattcat atgttagcc gcgtggcct ccacacaac gccactatt aaagcaagt ttcgcttat aattacctc ggccacttg caaaaactc ccaccaact gaggctgtt gttgaaatc atactttca aactgcaat ctcaaagtt ttagaaccg ggacctttt gataccacc gtcaatata aaatttctt gacttaagt cataatctg ctaacgtcg gaacaactt agcgccggt gtttttaag ggtccctac aatggggcc aaaccgact gagatagct ctcgaagag ctagatttg agctataat cagattcac agcctgcat aataacgtt tttaaattc gcgccgcat ctgaaacgg cttaatctc aaagggaat gactttgtc ttgttggat atacagaca caattggcc cttacttct ttaagaagt ctttcgagt ctgaatttg gccgataat gagctgacc gatttggtg gaaggtgca attgaagga ctggactat ttgcgcgag ttggatttg tcaatcaat tacttggat tttgtgccg catagtttg aaagcggtc gccaaaagt ctgcaagtg gtgaattta gacggaaat cctattatc gaaatgacc gaggaaagc tttcaaggc ttagaaaaa ctgatcgaa ttatctgcc aacaacttg accaaactt caccagatc aattcgagt gctttcgca cccgcccta agcttgaaa aaactaagc cttgcacac aacccttca ctctcagac atcaacgtc aacgctttc tcccccaac caatcaatc gaagagctg tacttaaac aacaacaat ctttcaacc cttcctttc gacctcatc ccttggcac cacttgacc agcttcgct ctcagcgac aacccccta acttgtagc tgcgacttg tacaaaatc gccacttcc cttccaagt tccataacc cgaacttca aaccatccc acctgtttc gacctttac acctcgacc accttcatg gtctacaac ctcgacagc gtcatctgc gacccccaa accctccca gctatcaaa cctcgaata ttacaccac cacttctcc atccccctc accggcctc atcgtggtc ctgtcactg gggctcttc gtagccatc gccttgggc tactcccga tataaaaaa ttcgcttcg gcgaccaga gacccatac gtatcgcag gtcgtctat agtccgctg ttcaatcgc gcggtctaa
- >TC034105 RA
tcaagtaca aaatgtctt ttcgtttgg ccatcctcc tgctacccc tcgcggtgg cttttggtg aagacatct gtcaaacct gtagttgca aagcaaaag ccaacgccc agatctaca caataaact gtgccaaca aaagcctag accacactc taacaacct ggccgcccc aaaacaaca caataatcg caaacttcg ccggcaaca acctaagga cacttgagc cgcttccac caacaaatg ccagtgttg agataatct tgtcacggt gtaacatca aatacctac aagccgcac tatttgccg ctgcgccaa acgtcaaat tcgttgatc ttagttaca atcaattgc cagccgaag aaatagcct cggaaaagt ttaggggac cgatcaaca acaccgagt atcggccaa tcgccgtcg aaaacctaa acctggcct acaatcaga tccacagcc tgggcatga acgttttcg aacatatgc ccaacttga aaatactta atttggaag gtaacgact ttaccatgt tggatatac acacacaac tggcattga cgtcggtta ggcaactgc agagtctca acctggcca ataacgaat taactgaaa tgatcgccg gtgccattc agactcaca aaaacttaa ccgagctga atttatcga acaatttgc ttgatttcg tcccgtcta gtttagccc agattggtc aaagtctgc aaatactca acctggaca ataacccga tcattgaat tgacatctg agtcgtttc taggcctcg gaaatataa tcgaactgt cggctaaca atttaacaa aactgaaat acgtcaatg ctaacactt ttgcctcca tgccgaaac taaaaaaac tgagtctct caaacaatt tccatttga gggacatag accgcgagg ctttcgccg ctaaccaaa ccctcgaag agttctatt tgaacaaca acgagctct tcaaactcg actttaagc tactgccat ggtcaaaat tacacattt ttgaattca aaaacaacc cgtttgatt gttcctgtg acttgtaca acatcaccc tggcgctgt ccaaagaca ttatccgat cagaggacg gcccttatt gcatggacc ctagaacgg accgcgaaa tgcaaattt tttacttga aaagtgata tctgctcag tcaagagca aacatgtga ctcggactg agtccatcg agtaccagt tttctgtcg ttaggataa gtttgattg tcttgagtg taattttga tgatgatag cgttagttg ctgcggttt tgggtgtga taaagtaca ggaggtacc agaggaata tgagctatc cgtacgcta cgcagattg cttgtggat actccgata acggccaaa ccctcactc tcgactgtt ctaagaaaa atttcattc atatgttag ccgcgtggc ctccacaca acgccacta ttaaagcaa gtttcgctt ataattacc tcggccact tgcaaaaac tcccaccaa ctgaggctg ttgttgaaa tcatacttt caaactgca atctcaaag ttttagaac cgggacctt ttgatacca ccgtcaata taaaatttc ttgacttaa gtcataatc tgctaacgt cggaacaac ttagcgccg gtgttttta agggtccct acaatgggg ccaaaccga ctgagatag ctctcgaag agctagatt tgagctata atcagattc acagcctgc ataataacg tttttaaat tcgcgccgc atctgaaac ggcttaatc tcaaaggga atgactttg tcttgttgg atatacaga cacaattgg cccttactt ctttaagaa gtctttcga gtctgaatt tggccgata atgagctga ccgatttgg tggaaggtg caattgaag gactggact atttgcgcg agttggatt tgtcaatca attacttgg attttgtgc cgcatagtt tgaaagcgg tcgccaaaa gtctgcaag tggtgaatt tagacggaa atcctatta tcgaaatga ccgaggaaa gctttcaag gcttagaaa aactgatcg aattatctg ccaacaact tgaccaaac ttcaccaga tcaattcga gtgctttcg cacccgccc taagcttga aaaaactaa gccttgcac acaaccctt cactctcag acatcaacg tcaacgctt tctccccca accaatcaa tcgaagagc tgtacttaa acaacaaca atctttcaa cccttcctt tcgacctca tcccttggc accacttga ccagcttcg ctctcagcg acaaccccc taacttgta gctgcgact tgtacaaaa tcgccactt cccttccaa gttccataa cccgaactt caaaccatc ccacctgtt tcgaccttt acacctcga ccaccttca tggtctaca acctcgaca gcgtcatct gcgaccccc aaaccctcc cagctatca aacctcgaa tattacacc accacttct ccatccccc tcaccggcc tcatcgtgg tcctgtcac tggggctct tcgtagcca tcgccttgg gctactccc gatataaaa aattcgctt cggcgacca gagacccat acgtatcgc aggtcgtct atagtccgc tgttcaatc gcgcggtct aaaataaaa tctcacctc tgctgttcc ggccagctg cttcgtata cgttaggaa ttggttgag gaggtcttc ggctttccg ggcgacctc tttgaacct gtagagagc ttcgagctg ttcgatgca tctcacaca gatgacgtg cggcagagg gtcgctctt ggcaacctg gaaagtgat cattgcggt gattaatgc gtgttgcgt aaattttcg t
