Status
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Gene information
General information
TC034105 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10459431..10463138
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034105
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034105 RA
      MSFRLAILL LPLAVAFGE DICQTCSCK AKANAQIYT 
INCANKSLD HTLTTWPPQ NNTIIANFA GNNLRTLEP 
LPPTNASVE IILSRCNIK YLQAALFAA APNVKFVDL 
SYNQLPAEE IASEKFRGP INNTEYRPI AVENLNLAY 
NQIHSLGMN VFEHMPNLK ILNLEGNDF TMLDIHTQL 
ALTSVRQLQ SLNLANNEL TEMIAGAIQ THKNLTELN 
LSNNLLDFV PSSLAQIGQ SLQILNLDN NPIIELTSE 
SFLGLGNII ELSANNLTK LKYVNANTF ASMPKLKKL 
SLSNNFHLR DIDREAFAA NQTLEEFYL NNNELFKLD 
FKLLPWSKL HIFEFKNNP FDCSCDLYN ITLALSKDI 
IRSEDGPYC MDPRTDREM QIFYLKSDI CSVKSKHVT 
RTESIEYQF SVVRISLIV LSVILMMIA LVAAVLGVI 
KYRRYQRNM SYPYATQIA CGYSDNGQT LTLDCSKKN 
FIHMLAAWP PHNATIKAS FAYNYLGHL QKLPPTEAV 
VEIILSNCN LKVLEPGPF DTTVNIKFL DLSHNLLTS 
EQLSAGVFK GPYNGAKPT EIALEELDL SYNQIHSLH 
NNVFKFAPH LKRLNLKGN DFVLLDIQT QLALTSLRS 
LSSLNLADN ELTDLVEGA IEGLDYLRE LDLSINYLD 
FVPHSLKAV AKSLQVVNL DGNPIIEMT EESFQGLEK 
LIELSANNL TKLHQINSS AFAPALSLK KLSLAHNPS 
LSDINVNAF SPNQSIEEL YLNNNNLST LPFDLIPWH 
HLTSFALSD NPLTCSCDL YKIATSLPS SITRTSNHP 
TCFDLYTST TFMVYNLDS VICDPQTLP AIKPRILHH 
HFSIPLTGL IVVLSLGLF VAIALGYSR YKKFASATR 
DPYVSQVVY SPLFNRAV
    CDS sequence
    • >TC034105 RA
      atgtctttt cgtttggcc atcctcctg ctacccctc 
gcggtggct tttggtgaa gacatctgt caaacctgt 
agttgcaaa gcaaaagcc aacgcccag atctacaca 
ataaactgt gccaacaaa agcctagac cacactcta 
acaacctgg ccgccccaa aacaacaca ataatcgca 
aacttcgcc ggcaacaac ctaaggaca cttgagccg 
cttccacca acaaatgcc agtgttgag ataatcttg 
tcacggtgt aacatcaaa tacctacaa gccgcacta 
tttgccgct gcgccaaac gtcaaattc gttgatctt 
agttacaat caattgcca gccgaagaa atagcctcg 
gaaaagttt aggggaccg atcaacaac accgagtat 
cggccaatc gccgtcgaa aacctaaac ctggcctac 
aatcagatc cacagcctg ggcatgaac gttttcgaa 
catatgccc aacttgaaa atacttaat ttggaaggt 
aacgacttt accatgttg gatatacac acacaactg 
gcattgacg tcggttagg caactgcag agtctcaac 
ctggccaat aacgaatta actgaaatg atcgccggt 
gccattcag actcacaaa aacttaacc gagctgaat 
ttatcgaac aatttgctt gatttcgtc ccgtctagt 
ttagcccag attggtcaa agtctgcaa atactcaac 
ctggacaat aacccgatc attgaattg acatctgag 
tcgtttcta ggcctcgga aatataatc gaactgtcg 
gctaacaat ttaacaaaa ctgaaatac gtcaatgct 
aacactttt gcctccatg ccgaaacta aaaaaactg 
agtctctca aacaatttc catttgagg gacatagac 
cgcgaggct ttcgccgct aaccaaacc ctcgaagag 
ttctatttg aacaacaac gagctcttc aaactcgac 
tttaagcta ctgccatgg tcaaaatta cacattttt 
gaattcaaa aacaacccg tttgattgt tcctgtgac 
ttgtacaac atcaccctg gcgctgtcc aaagacatt 
atccgatca gaggacggc ccttattgc atggaccct 
agaacggac cgcgaaatg caaattttt tacttgaaa 
agtgatatc tgctcagtc aagagcaaa catgtgact 
cggactgag tccatcgag taccagttt tctgtcgtt 
aggataagt ttgattgtc ttgagtgta attttgatg 
atgatagcg ttagttgct gcggttttg ggtgtgata 
aagtacagg aggtaccag aggaatatg agctatccg 
tacgctacg cagattgct tgtggatac tccgataac 
ggccaaacc ctcactctc gactgttct aagaaaaat 
ttcattcat atgttagcc gcgtggcct ccacacaac 
gccactatt aaagcaagt ttcgcttat aattacctc 
ggccacttg caaaaactc ccaccaact gaggctgtt 
gttgaaatc atactttca aactgcaat ctcaaagtt 
ttagaaccg ggacctttt gataccacc gtcaatata 
aaatttctt gacttaagt cataatctg ctaacgtcg 
gaacaactt agcgccggt gtttttaag ggtccctac 
aatggggcc aaaccgact gagatagct ctcgaagag 
ctagatttg agctataat cagattcac agcctgcat 
aataacgtt tttaaattc gcgccgcat ctgaaacgg 
cttaatctc aaagggaat gactttgtc ttgttggat 
atacagaca caattggcc cttacttct ttaagaagt 
ctttcgagt ctgaatttg gccgataat gagctgacc 
gatttggtg gaaggtgca attgaagga ctggactat 
ttgcgcgag ttggatttg tcaatcaat tacttggat 
tttgtgccg catagtttg aaagcggtc gccaaaagt 
ctgcaagtg gtgaattta gacggaaat cctattatc 
gaaatgacc gaggaaagc tttcaaggc ttagaaaaa 
ctgatcgaa ttatctgcc aacaacttg accaaactt 
caccagatc aattcgagt gctttcgca cccgcccta 
agcttgaaa aaactaagc cttgcacac aacccttca 
ctctcagac atcaacgtc aacgctttc tcccccaac 
caatcaatc gaagagctg tacttaaac aacaacaat 
ctttcaacc cttcctttc gacctcatc ccttggcac 
cacttgacc agcttcgct ctcagcgac aacccccta 
acttgtagc tgcgacttg tacaaaatc gccacttcc 
cttccaagt tccataacc cgaacttca aaccatccc 
acctgtttc gacctttac acctcgacc accttcatg 
gtctacaac ctcgacagc gtcatctgc gacccccaa 
accctccca gctatcaaa cctcgaata ttacaccac 
cacttctcc atccccctc accggcctc atcgtggtc 
ctgtcactg gggctcttc gtagccatc gccttgggc 
tactcccga tataaaaaa ttcgcttcg gcgaccaga 
gacccatac gtatcgcag gtcgtctat agtccgctg 
ttcaatcgc gcggtctaa
    mRNA sequence
    • >TC034105 RA
      tcaagtaca aaatgtctt ttcgtttgg ccatcctcc 
tgctacccc tcgcggtgg cttttggtg aagacatct 
gtcaaacct gtagttgca aagcaaaag ccaacgccc 
agatctaca caataaact gtgccaaca aaagcctag 
accacactc taacaacct ggccgcccc aaaacaaca 
caataatcg caaacttcg ccggcaaca acctaagga 
cacttgagc cgcttccac caacaaatg ccagtgttg 
agataatct tgtcacggt gtaacatca aatacctac 
aagccgcac tatttgccg ctgcgccaa acgtcaaat 
tcgttgatc ttagttaca atcaattgc cagccgaag 
aaatagcct cggaaaagt ttaggggac cgatcaaca 
acaccgagt atcggccaa tcgccgtcg aaaacctaa 
acctggcct acaatcaga tccacagcc tgggcatga 
acgttttcg aacatatgc ccaacttga aaatactta 
atttggaag gtaacgact ttaccatgt tggatatac 
acacacaac tggcattga cgtcggtta ggcaactgc 
agagtctca acctggcca ataacgaat taactgaaa 
tgatcgccg gtgccattc agactcaca aaaacttaa 
ccgagctga atttatcga acaatttgc ttgatttcg 
tcccgtcta gtttagccc agattggtc aaagtctgc 
aaatactca acctggaca ataacccga tcattgaat 
tgacatctg agtcgtttc taggcctcg gaaatataa 
tcgaactgt cggctaaca atttaacaa aactgaaat 
acgtcaatg ctaacactt ttgcctcca tgccgaaac 
taaaaaaac tgagtctct caaacaatt tccatttga 
gggacatag accgcgagg ctttcgccg ctaaccaaa 
ccctcgaag agttctatt tgaacaaca acgagctct 
tcaaactcg actttaagc tactgccat ggtcaaaat 
tacacattt ttgaattca aaaacaacc cgtttgatt 
gttcctgtg acttgtaca acatcaccc tggcgctgt 
ccaaagaca ttatccgat cagaggacg gcccttatt 
gcatggacc ctagaacgg accgcgaaa tgcaaattt 
tttacttga aaagtgata tctgctcag tcaagagca 
aacatgtga ctcggactg agtccatcg agtaccagt 
tttctgtcg ttaggataa gtttgattg tcttgagtg 
taattttga tgatgatag cgttagttg ctgcggttt 
tgggtgtga taaagtaca ggaggtacc agaggaata 
tgagctatc cgtacgcta cgcagattg cttgtggat 
actccgata acggccaaa ccctcactc tcgactgtt 
ctaagaaaa atttcattc atatgttag ccgcgtggc 
ctccacaca acgccacta ttaaagcaa gtttcgctt 
ataattacc tcggccact tgcaaaaac tcccaccaa 
ctgaggctg ttgttgaaa tcatacttt caaactgca 
atctcaaag ttttagaac cgggacctt ttgatacca 
ccgtcaata taaaatttc ttgacttaa gtcataatc 
tgctaacgt cggaacaac ttagcgccg gtgttttta 
agggtccct acaatgggg ccaaaccga ctgagatag 
ctctcgaag agctagatt tgagctata atcagattc 
acagcctgc ataataacg tttttaaat tcgcgccgc 
atctgaaac ggcttaatc tcaaaggga atgactttg 
tcttgttgg atatacaga cacaattgg cccttactt 
ctttaagaa gtctttcga gtctgaatt tggccgata 
atgagctga ccgatttgg tggaaggtg caattgaag 
gactggact atttgcgcg agttggatt tgtcaatca 
attacttgg attttgtgc cgcatagtt tgaaagcgg 
tcgccaaaa gtctgcaag tggtgaatt tagacggaa 
atcctatta tcgaaatga ccgaggaaa gctttcaag 
gcttagaaa aactgatcg aattatctg ccaacaact 
tgaccaaac ttcaccaga tcaattcga gtgctttcg 
cacccgccc taagcttga aaaaactaa gccttgcac 
acaaccctt cactctcag acatcaacg tcaacgctt 
tctccccca accaatcaa tcgaagagc tgtacttaa 
acaacaaca atctttcaa cccttcctt tcgacctca 
tcccttggc accacttga ccagcttcg ctctcagcg 
acaaccccc taacttgta gctgcgact tgtacaaaa 
tcgccactt cccttccaa gttccataa cccgaactt 
caaaccatc ccacctgtt tcgaccttt acacctcga 
ccaccttca tggtctaca acctcgaca gcgtcatct 
gcgaccccc aaaccctcc cagctatca aacctcgaa 
tattacacc accacttct ccatccccc tcaccggcc 
tcatcgtgg tcctgtcac tggggctct tcgtagcca 
tcgccttgg gctactccc gatataaaa aattcgctt 
cggcgacca gagacccat acgtatcgc aggtcgtct 
atagtccgc tgttcaatc gcgcggtct aaaataaaa 
tctcacctc tgctgttcc ggccagctg cttcgtata 
cgttaggaa ttggttgag gaggtcttc ggctttccg 
ggcgacctc tttgaacct gtagagagc ttcgagctg 
ttcgatgca tctcacaca gatgacgtg cggcagagg 
gtcgctctt ggcaacctg gaaagtgat cattgcggt 
gattaatgc gtgttgcgt aaattttcg t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY