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Gene information
General information
TC034102 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10427389..10431218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC034102
      iBeetle screen iB_07520

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034102 RA
        MVLSRIQMK AKTLLVFLQ ILIPRLVLH 
FPTQRQKAL VLH
      CDS sequence
      • >TC034102 RA
        atggtgttg agccgaatt caaatgaaa 
gcgaaaact ttactagtt tttcttcaa 
atactgatt ccgcgatta gttctccac 
tttccgact caacgtcag aaagcccta 
gttttgcat taa
      mRNA sequence
      • >TC034102 RA
        agttaaaag taggtaccg tatgaaatg aagcgagta 
ttgcttttt ttgattgta ctgcaatta agttcatcg 
caaaggtgt ccagtttgt aaagattat tattgtttg 
gaaaacatt tggaaagat tgccctcaa acactggtt 
aattcaaat attacaaat ataagcaat gcaagtatt 
aattgcatc agtgtgcag tacataggt gattagaaa 
ttgtttgaa acacaccca tatatcctg tttcttaac 
agcccaaca agagtatcg ccaattgaa ggactgtgt 
tattggaac ggaagcgga aaattgtca attatttta 
ccaagaata cggaataca aaacgttaa ttgtacggt 
cgtcccagc atttgttga tattcaacc gtcttcaaa 
tcaattaga aagaaatag acttgcaaa cgggacaag 
tactggtca aggtttaaa tttaaatct aaactgtag 
tagtccatg tttgaattt aaatttaag tttattttt 
aagtgatga ttcgaattc gacttctag catcagttc 
tagtgaaag tctcagtta tagttacga tgaaactct 
taatgttag ttacttaaa ttttaattt ttcgtcaaa 
ttcgtttgg caatttaag ttttaattt gacagaaga 
ttcaagtga ctcagattt aaattacag ctatgattt 
aattttcgc cacaaattc aagtgttaa tttaaacga 
aagtgactt gttgctgtc taatttaaa tccaattcc 
aattccgat ttacagtac ttctctaaa tcaaagttt 
aaatttgac ttcatctga agaagactc gggttctga 
tttgagcta cagttacga ctggaattc aaattcaag 
ctttaattt gacttccaa tgaaactga ttttttgtc 
ttctgatcc tgtcattat cagttcaaa cccagttct 
aggttacag tggaaactc aacttcaaa tagcagtgt 
tagttttac ttcggctga agataattc taactctga 
actgaattc aagtgcgaa cctaaattt aattctaaa 
caaaaatta ttctatgtc ttctgacat cattttaaa 
tactacgtc ctacgaaaa tttaagttt cagttcgac 
agaacaaga atcaaattc cagttttaa tcccatttt 
acaaacaaa ttcaaacct gaatgtaga ttttaacgt 
aagtcaagc cagtattga gaataataa tagttatgc 
tgaccataa ttttactga ctctgaact aagctcaag 
tttagagtt tttcataaa ctcaagtca aaacttgcc 
attggcttc taattcaga aatagattt aagtttaaa 
ctcaagttt gcaaaattt agaacaagc aaacacaac 
ggaaagaat actccaaac aaataattc tgattttga 
taaaatggt tacttttag tccaaattc tacagagca 
aactttaac ccccactga accaacgat aagtcttct 
atctttttt caacacgac acctaatct actctctga 
tgcaacagt tttgtctga aagtcctat attaagcac 
aacttccga ttatgaatt gagtgctga aacaaatag 
cttaacctt gacagatct tagctccgt tagttctac 
cttgccttc agactcaag tctcggccc atatcaaga 
ttttagtcc aacttccaa tattttaat attcgatgg 
tgttgagcc gaattcaaa tgaaagcga aaactttac 
tagtttttc ttcaaatac tgattccgc gattagttc 
tccactttc cgactcaac gtcagaaag ccctagttt 
tgcattaag ttctggttt agctaattc gaccacttc 
gaactttga cgtaagccc aactttggc cttaaatcc 
gaatttcag ctatagccc aaaccaggg ttcaacagt 
tactccaac ttcttataa tgaaaatta ttccttaag 
tattacgta taccagccc aacttctaa tttttcaga 
tttagaaac tatttctcc tttcgacaa ttatagttt 
tacttcaag tcctggttt gaatttaag ttcagctga 
cagtttgga tgtaacttc tagttcttc taacccaac 
tgaaagttt caaaattag tttaactac cagtcctac 
ttctaatac taatagctt tgattctag tacaaccac 
tgaaccggt tttaaggcc aggttttgc tacaagacc 
aggtttctt gacccaaaa ttcaggttt gacaactcc 
aacttcgcg ttctaattc aagctttag aattgaccc 
taatatcag ttttccttc aagtgacaa ttcaaccag 
aagtaactt tacttctaa cacaactcc aagttttta 
tacaatttt gcgcgacat gttcagatt caatgttag 
cacgacacc aagctttcg taaaaagct taatgtaac 
ttctcgccc tcatcctac aagccctag ctacacttc 
cagttttac gttcaactg gatccctga tagacacgt 
tcttccctt tattcctac tccaagtgt aaacggtaa 
taagcctaa aaaaccggc ttctcaagt cagcccaga 
cctgaagcc aagtccaga ttctgcgag cattaacac 
agttaacgt aatcataga aatcctagt tttaatgct 
atctgtttt tgaactctg ttaattagt taagttggt 
acttatcgt tgtagattg ttgtaaaat aagttattt 
tttactctt atttgtaaa tatgtatat ttttctaac 
tgtactcat atcgtgata aatgttgcc aattaagtt 
taaagtttg tcacattaa agataatct tttctggtc 
aatcgactt tccaccctt cgatgcacg caatgcacg 
taatagcat tctgaggtg aacaaacta gtctgcatt 
cgaaaatca tgcaaatga gacgcgatg actaccatc 
tcctgcatt gtagtgaca actctagtg gttgtgtct 
ttggctaat ttagtgctt ctagtgcac gttattatg 
aattcaatc tacaaatgt agtcaacac cggacgtat 
ttgaaacgc gttggaaaa atagaaaaa cgggaagat 
tcatctttt atggatcaa gagtttaaa acaacagca 
aacaatttt gtggctgct ttaattgta gaaattgac 
gatgataat tttgagttt gtgtgtgga gtggctatt 
gtgggcaaa aaatgggct ctaacacca gcacattgt 
gtggatgct atcgttgat caacctcta actaaagtt 
aagatttct tctaacact ttgaagctg aaatacgga 
atttggtac gacgttgat agaatgagt aaacacgaa 
aattataag tctagtgaa cacgtaaac gacatagct 
ttgataagt gttaagcaa tcatttaaa gacaaagaa 
gttaaaagt gttatcctg ccgactaga aatttttta 
tacgtagct gagtcctca gcaactgct attgggtgg 
ttgggtcaa gaagtgttt gcagttgat ttgaaactg 
cttccacat aaacagtgt cagagtgct tatctcgaa 
ggagttaca aatggtact ttttgtgcc gaaagaaaa 
gcttgtgtt tatgaccat ggagggttt ttgattcag 
aaaggtgtg ctaattggg actatctct tttggggta 
ggatgtaac tcaaaaccg gttgtttat acgaaaatc 
tcgttgttt gaagattgg ttcaaaggt cggaaaatc 
ggagcagtt tttaaagga agtacttaa catgtattc 
tcgcaattg tagacacag aaataaagt ttataacga 
ttt
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