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Gene information
General information
TC034102 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10427389..10431218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034102
      iBeetle screen iB_07520

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034102 RA
        MVLSRIQMK AKTLLVFLQ ILIPRLVLH 
        FPTQRQKAL VLH
      CDS sequence
      • >TC034102 RA
        atggtgttg agccgaatt caaatgaaa 
        gcgaaaact ttactagtt tttcttcaa 
        atactgatt ccgcgatta gttctccac 
        tttccgact caacgtcag aaagcccta 
        gttttgcat taa
      mRNA sequence
      • >TC034102 RA
        agttaaaag taggtaccg tatgaaatg aagcgagta 
        ttgcttttt ttgattgta ctgcaatta agttcatcg 
        caaaggtgt ccagtttgt aaagattat tattgtttg 
        gaaaacatt tggaaagat tgccctcaa acactggtt 
        aattcaaat attacaaat ataagcaat gcaagtatt 
        aattgcatc agtgtgcag tacataggt gattagaaa 
        ttgtttgaa acacaccca tatatcctg tttcttaac 
        agcccaaca agagtatcg ccaattgaa ggactgtgt 
        tattggaac ggaagcgga aaattgtca attatttta 
        ccaagaata cggaataca aaacgttaa ttgtacggt 
        cgtcccagc atttgttga tattcaacc gtcttcaaa 
        tcaattaga aagaaatag acttgcaaa cgggacaag 
        tactggtca aggtttaaa tttaaatct aaactgtag 
        tagtccatg tttgaattt aaatttaag tttattttt 
        aagtgatga ttcgaattc gacttctag catcagttc 
        tagtgaaag tctcagtta tagttacga tgaaactct 
        taatgttag ttacttaaa ttttaattt ttcgtcaaa 
        ttcgtttgg caatttaag ttttaattt gacagaaga 
        ttcaagtga ctcagattt aaattacag ctatgattt 
        aattttcgc cacaaattc aagtgttaa tttaaacga 
        aagtgactt gttgctgtc taatttaaa tccaattcc 
        aattccgat ttacagtac ttctctaaa tcaaagttt 
        aaatttgac ttcatctga agaagactc gggttctga 
        tttgagcta cagttacga ctggaattc aaattcaag 
        ctttaattt gacttccaa tgaaactga ttttttgtc 
        ttctgatcc tgtcattat cagttcaaa cccagttct 
        aggttacag tggaaactc aacttcaaa tagcagtgt 
        tagttttac ttcggctga agataattc taactctga 
        actgaattc aagtgcgaa cctaaattt aattctaaa 
        caaaaatta ttctatgtc ttctgacat cattttaaa 
        tactacgtc ctacgaaaa tttaagttt cagttcgac 
        agaacaaga atcaaattc cagttttaa tcccatttt 
        acaaacaaa ttcaaacct gaatgtaga ttttaacgt 
        aagtcaagc cagtattga gaataataa tagttatgc 
        tgaccataa ttttactga ctctgaact aagctcaag 
        tttagagtt tttcataaa ctcaagtca aaacttgcc 
        attggcttc taattcaga aatagattt aagtttaaa 
        ctcaagttt gcaaaattt agaacaagc aaacacaac 
        ggaaagaat actccaaac aaataattc tgattttga 
        taaaatggt tacttttag tccaaattc tacagagca 
        aactttaac ccccactga accaacgat aagtcttct 
        atctttttt caacacgac acctaatct actctctga 
        tgcaacagt tttgtctga aagtcctat attaagcac 
        aacttccga ttatgaatt gagtgctga aacaaatag 
        cttaacctt gacagatct tagctccgt tagttctac 
        cttgccttc agactcaag tctcggccc atatcaaga 
        ttttagtcc aacttccaa tattttaat attcgatgg 
        tgttgagcc gaattcaaa tgaaagcga aaactttac 
        tagtttttc ttcaaatac tgattccgc gattagttc 
        tccactttc cgactcaac gtcagaaag ccctagttt 
        tgcattaag ttctggttt agctaattc gaccacttc 
        gaactttga cgtaagccc aactttggc cttaaatcc 
        gaatttcag ctatagccc aaaccaggg ttcaacagt 
        tactccaac ttcttataa tgaaaatta ttccttaag 
        tattacgta taccagccc aacttctaa tttttcaga 
        tttagaaac tatttctcc tttcgacaa ttatagttt 
        tacttcaag tcctggttt gaatttaag ttcagctga 
        cagtttgga tgtaacttc tagttcttc taacccaac 
        tgaaagttt caaaattag tttaactac cagtcctac 
        ttctaatac taatagctt tgattctag tacaaccac 
        tgaaccggt tttaaggcc aggttttgc tacaagacc 
        aggtttctt gacccaaaa ttcaggttt gacaactcc 
        aacttcgcg ttctaattc aagctttag aattgaccc 
        taatatcag ttttccttc aagtgacaa ttcaaccag 
        aagtaactt tacttctaa cacaactcc aagttttta 
        tacaatttt gcgcgacat gttcagatt caatgttag 
        cacgacacc aagctttcg taaaaagct taatgtaac 
        ttctcgccc tcatcctac aagccctag ctacacttc 
        cagttttac gttcaactg gatccctga tagacacgt 
        tcttccctt tattcctac tccaagtgt aaacggtaa 
        taagcctaa aaaaccggc ttctcaagt cagcccaga 
        cctgaagcc aagtccaga ttctgcgag cattaacac 
        agttaacgt aatcataga aatcctagt tttaatgct 
        atctgtttt tgaactctg ttaattagt taagttggt 
        acttatcgt tgtagattg ttgtaaaat aagttattt 
        tttactctt atttgtaaa tatgtatat ttttctaac 
        tgtactcat atcgtgata aatgttgcc aattaagtt 
        taaagtttg tcacattaa agataatct tttctggtc 
        aatcgactt tccaccctt cgatgcacg caatgcacg 
        taatagcat tctgaggtg aacaaacta gtctgcatt 
        cgaaaatca tgcaaatga gacgcgatg actaccatc 
        tcctgcatt gtagtgaca actctagtg gttgtgtct 
        ttggctaat ttagtgctt ctagtgcac gttattatg 
        aattcaatc tacaaatgt agtcaacac cggacgtat 
        ttgaaacgc gttggaaaa atagaaaaa cgggaagat 
        tcatctttt atggatcaa gagtttaaa acaacagca 
        aacaatttt gtggctgct ttaattgta gaaattgac 
        gatgataat tttgagttt gtgtgtgga gtggctatt 
        gtgggcaaa aaatgggct ctaacacca gcacattgt 
        gtggatgct atcgttgat caacctcta actaaagtt 
        aagatttct tctaacact ttgaagctg aaatacgga 
        atttggtac gacgttgat agaatgagt aaacacgaa 
        aattataag tctagtgaa cacgtaaac gacatagct 
        ttgataagt gttaagcaa tcatttaaa gacaaagaa 
        gttaaaagt gttatcctg ccgactaga aatttttta 
        tacgtagct gagtcctca gcaactgct attgggtgg 
        ttgggtcaa gaagtgttt gcagttgat ttgaaactg 
        cttccacat aaacagtgt cagagtgct tatctcgaa 
        ggagttaca aatggtact ttttgtgcc gaaagaaaa 
        gcttgtgtt tatgaccat ggagggttt ttgattcag 
        aaaggtgtg ctaattggg actatctct tttggggta 
        ggatgtaac tcaaaaccg gttgtttat acgaaaatc 
        tcgttgttt gaagattgg ttcaaaggt cggaaaatc 
        ggagcagtt tttaaagga agtacttaa catgtattc 
        tcgcaattg tagacacag aaataaagt ttataacga 
        ttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY