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Gene information
General information
TC005670 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9834183..9860399
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005670
    iBeetle screen iB_07509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005670 RA
      MQLCLILLV SSVLIIASE ARHFYPYST HPPLYHPVA 
      TTPRPHHQR NINDVTVVD EKTGIRYNP YSKHHPGRH 
      VCANQRNVS YPIRTKRVF AKPTYKKYM VRCGTQWCE 
      GVRLVYEKY HREVVTDAN HTLVDYTCC PGWTQVNYK 
      SHGCNKPVC SQPCTNGGK CVRPNVCMC PPGYDGTYC 
      ETKTTVYSP DGSCPNGQT VCLCQTLGP SYCPHHWFC 
      SNLCPAHVC IPCPQSQCT CPCPSKHNG LQDDDRPVC 
      PVCLNGGTC RPPGCVCPP GFTGIQCEN AQIQTDCSL 
      PCKNGGTCK TKSVCICPP GFSGKLCDK DVDECKQKP 
      CDQICYNTP GSYRCECKE SFVLQRDGQ TCRKENDDD 
      TALEAKDLD TQQKLDKMD KRLQMLEKM VKEKSKNEV 
      TKQDLKSIY GNIDRMSSD IGSIACKLR RLESCNQDK 
      TPPKSCIDL VENTIHSNT KPPSPADEA SCDCEKPQE 
      HPPGCKCMS SSV
    CDS sequence
    • >TC005670 RA
      atgcagttg tgtcttata ctgctggtg tcttctgtc 
      ttgattatc gcgagtgag gcacgacat ttctaccct 
      tactcaact caccctcca ctctatcat ccggtcgca 
      acgacccct cgacctcac catcaaaga aacatcaat 
      gatgtgacg gtggtcgac gaaaaaaca gggatacga 
      tacaacccc tacagcaag caccatccg gggagacat 
      gtttgcgca aatcaaaga aacgtcagt taccccatt 
      cgaacaaaa cgcgtcttt gccaaaccc acctacaag 
      aaatacatg gtacgctgt ggcacccaa tggtgtgaa 
      ggtgttagg ttagtgtat gaaaaatac catagggaa 
      gttgtcaca gacgccaac cacactcta gtggactac 
      acctgctgc ccaggatgg acacaggtc aattacaag 
      agtcacggc tgtaataaa cctgtgtgc tcgcagccc 
      tgcaccaac ggaggcaag tgtgtaaga cccaacgtg 
      tgcatgtgt cccccggga tacgacggc acgtattgt 
      gaaactaaa accactgtg tacagcccc gatgggagt 
      tgtcccaat ggtcaaact gtctgtctt tgtcaaact 
      ttgggcccg tcctattgt ccccaccat tggttttgt 
      tctaatttg tgtcctgct cacgtgtgc atcccctgt 
      ccacaatcc cagtgcact tgtccttgt ccttccaaa 
      cataatggg ttgcaagac gacgacaga cccgtgtgt 
      cctgtgtgt ctaaatggg ggaacctgt cggcctccg 
      ggttgtgtt tgtcctccg ggatttacc ggaattcaa 
      tgtgaaaat gcacagatt caaacggac tgcagtctg 
      ccgtgcaag aacggcggc acttgcaag accaagtcc 
      gtgtgcatt tgtccacca ggcttcagt ggtaaactc 
      tgcgacaaa gacgtggat gagtgcaag cagaagccc 
      tgcgaccaa atatgttac aacacacct gggagctac 
      cgctgcgaa tgcaaagag tcgttcgta ttgcaaaga 
      gacggtcag acttgtcgt aaagagaac gacgacgac 
      acagcgctc gaggccaaa gatttggat acacagcaa 
      aaattggat aagatggat aaacggctg cagatgctc 
      gaaaagatg gtgaaagaa aagagcaaa aacgaagtt 
      acgaagcag gatcttaaa agtatttat ggaaacatt 
      gataggatg tctagtgat attggtagt atagcgtgc 
      aagttgcgg cgattggaa agctgtaat caagataaa 
      acgccacca aaaagttgc atcgatctt gtagaaaac 
      acaattcat agtaacacc aaaccccct tctccagcc 
      gacgaagca agctgtgat tgtgaaaaa ccacaagaa 
      catccccca gggtgtaaa tgtatgtcg tcgtcagtg 
      taa
    mRNA sequence
    • >TC005670 RA
      cattcatcg taatgcacc gatgcagtt ttatcacgt 
      gatcggtgg taccggcga aagaactca cgtgttgag 
      accctaaag cagtacgca caatgcagt tgtgtctta 
      tactgctgg tgtcttctg tcttgatta tcgcgagtg 
      aggcacgac atttctacc cttactcaa ctcaccctc 
      cactctatc atccggtcg caacgaccc ctcgacctc 
      accatcaaa gaaacatca atgatgtga cggtggtcg 
      acgaaaaaa cagggatac gatacaacc cctacagca 
      agcaccatc cggggagac atgtttgcg caaatcaaa 
      gaaacgtca gttacccca ttcgaacaa aacgcgtct 
      ttgccaaac ccacctaca agaaataca tggtacgct 
      gtggcaccc aatggtgtg aaggtgtta ggttagtgt 
      atgaaaaat accataggg aagttgtca cagacgcca 
      accacactc tagtggact acacctgct gcccaggat 
      ggacacagg tcaattaca agagtcacg gctgtaata 
      aacctgtgt gctcgcagc cctgcacca acggaggca 
      agtgtgtaa gacccaacg tgtgcatgt gtcccccgg 
      gatacgacg gcacgtatt gtgaaacta aaaccactg 
      tgtacagcc ccgatggga gttgtccca atggtcaaa 
      ctgtctgtc tttgtcaaa ctttgggcc cgtcctatt 
      gtccccacc attggtttt gttctaatt tgtgtcctg 
      ctcacgtgt gcatcccct gtccacaat cccagtgca 
      cttgtcctt gtccttcca aacataatg ggttgcaag 
      acgacgaca gacccgtgt gtcctgtgt gtctaaatg 
      ggggaacct gtcggcctc cgggttgtg tttgtcctc 
      cgggattta ccggaattc aatgtgaaa atgcacaga 
      ttcaaacgg actgcagtc tgccgtgca agaacggcg 
      gcacttgca agaccaagt ccgtgtgca tttgtccac 
      caggcttca gtggtaaac tctgcgaca aagacgtgg 
      atgagtgca agcagaagc cctgcgacc aaatatgtt 
      acaacacac ctgggagct accgctgcg aatgcaaag 
      agtcgttcg tattgcaaa gagacggtc agacttgtc 
      gtaaagaga acgacgacg acacagcgc tcgaggcca 
      aagatttgg atacacagc aaaaattgg ataagatgg 
      ataaacggc tgcagatgc tcgaaaaga tggtgaaag 
      aaaagagca aaaacgaag ttacgaagc aggatctta 
      aaagtattt atggaaaca ttgatagga tgtctagtg 
      atattggta gtatagcgt gcaagttgc ggcgattgg 
      aaagctgta atcaagata aaacgccac caaaaagtt 
      gcatcgatc ttgtagaaa acacaattc atagtaaca 
      ccaaacccc cttctccag ccgacgaag caagctgtg 
      attgtgaaa aaccacaag aacatcccc cagggtgta 
      aatgtatgt cgtcgtcag tgtaaaaat ttgaactta 
      tatttatat gtaaaaaca caaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY