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Gene information
General information
TC006451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9810026..9820790
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006451
    iBeetle screen iB_07508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006451 RA
      MAESCPSGR SNFNPLSSI NRDSELPKQ DCTSPSPTE 
      TVCLESLCT TDNAPGTSQ NHLAPLSEK LDEIFRISQ 
      SGEETEKTS FLNKLGLEE CDEEDSFEK EENWEDCTE 
      GDLSEENQE LEEDEDSSE VFSGSPPGP HQTTPRSSY 
      AKRSGSYAT DRYWRKRKL DVQNGFPCK KLLPEKKSD 
      VTSMSSEPS ARAPTAPET NGATPSDKK SRLVIPTKD 
      NPPPEMSDW LTQFQKWSN AERILAISE LIARCEPTQ 
      VRHMMQVIE PQFQRDFIS LLPRELALN VLSFLEPKD 
      LLRAAQTCR SWRFLAEDN LLWKEKCRQ AGIDEIPKR 
      RCSPRSPGT QMSPWKAAY MRQHAIEMN WRSKPIRPP 
      KMLKGHDDH VITCLQFCG NRIVSGSDD NTLKVWSAI 
      TGKCLRTLV GHTGGVWSS QMSGATIIS GSTDRTLKV 
      WDAETGDCI HTLNGHTST VRCMHLHGN KVVSGSRDA 
      TLRVWDIKT GACLHVLVG HLAAVRCVQ YDGRLVVSG 
      AYDYMVKVW NPEREECLH TLQGHTNRV YSLQFDGVH 
      VVSGSLDTS IRVWEVETG ACKHTLMGH QSLTSGMEL 
      RNNILVSGN ADSTVKVWD IVTGQCLQT LSGPYKHQS 
      AVTCLQFNN RFVITSSDD GTVKLWDVR TGEFIRNLV 
      ALDSGGSGG VVWRIRAND TKLVCAVGS RNGTEETKL 
      LVLDFDVDS NSSTIISR
    • >TC006451 RB
      MAESCPSGR SNFNPLSSI NRDSELPKQ DCTSPSPTE 
      TVCLESLCT TDNAPGTSQ NHLAPLSEK LDEIFRISQ 
      SGEETEKTS FLNKLGLEE CDEEDSFEK EENWEDCTE 
      GDLSEENQE LEEDEDSSE VFSGSPPGP HQTTPRSSY 
      AKRSGSYAT DRYWRKRKL DVQNGFPCK KLLPEKKSD 
      VTSMSSEPS ARAPTAPET NGATPSDKK SRLVIPTKD 
      NPPPEMSDW LTQFQKWSN AERILAISE LIARCEPTQ 
      VRHMMQVIE PQFQRDFIS LLPRELALN VLSFLEPKD 
      LLRAAQTCR SWRFLAEDN LLWKEKCRQ AGIDEIPKR 
      RCSPRSPGT QMSPWKAAY MRQHAIEMN WRSKPIRPP 
      KMLKGHDDH VITCLQFCG NRIVSGSDD NTLKVWSAI 
      TGKCLRTLV GHTGGVWSS QMSGATIIS GSTDRTLKV 
      WDAETGDCI HTLNGHTST VRCMHLHGN KVVSGSRDA 
      TLRVWDIKT GACLHVLVG HLAAVRCVQ YDGRLVVSG 
      AYDYMVKVW NPEREECLH TLQGHTNRV YSLQFDGVH 
      VVSGSLDTS IRVWEVETG ACKHTLMGH QSLTSGMEL 
      RNNILVSGN ADSTVKVWD IVTGQCLQT LSGPYKHQS 
      AVTCLQFNN RFVITSSDD GTVKLWDVR TGEFIRNLV 
      ALDSGGSGG VVWRIRAND TKLVCAVGS RNGTEETKL 
      LVLDFDVDS NSSTIISS
    CDS sequence
    • >TC006451 RA
      atggcagaa tcatgtcct tcaggcaga agtaatttc 
      aacccctta tctagtatt aatcgagat tcagaactt 
      ccaaaacaa gactgcacc agccctagc cctactgaa 
      accgtttgt ctagagtcg ttgtgtacc actgataat 
      gcaccaggt acatcccag aatcactta gctccattg 
      agtgaaaaa ctagatgag atatttaga attagtcaa 
      tcgggcgag gagactgaa aaaactagc tttctcaat 
      aagctaggt ctggaagaa tgtgatgaa gaagacagt 
      tttgagaag gaagaaaac tgggaggat tgtactgaa 
      ggagacctc tctgaagaa aatcaagag ctagaagag 
      gacgaagat agcagtgaa gttttttca ggttcacct 
      ccaggtcct catcaaaca acaccaagg agttcgtac 
      gcgaagaga agtgggtcg tacgccact gacagatac 
      tggagaaaa cggaagttg gacgtgcag aatgggttt 
      ccgtgcaag aagttgcta ccagaaaag aaatctgac 
      gttacttcg atgtcatct gagccctct gctcgtgcg 
      ccgacggcg cccgaaacc aacggtgcc acccctagc 
      gacaaaaaa tctcgtctc gtaatcccg accaaagac 
      aatccgcca ccggaaatg tccgattgg ctgacccaa 
      ttccaaaaa tggtcaaac gcggaacga attctcgca 
      atcagcgaa ctaatcgcc cgctgcgaa ccaactcaa 
      gtccgtcat atgatgcaa gtgattgaa ccacaattc 
      caacgagat ttcatctca ttattacca cgcgagttg 
      gctttgaac gttttatca tttctcgaa ccgaaagac 
      cttttacgt gcagcacaa acttgtcgc agttggcgt 
      ttcctggct gaagataat ctactgtgg aaggaaaag 
      tgtcggcaa gcaggcatt gatgaaata cctaaacgg 
      aggtgctca cctaggtct cctggaacg caaatgtcg 
      ccatggaaa gctgcctac atgagacaa catgccatt 
      gaaatgaac tggaggagc aaaccgatc aggccgcct 
      aagatgcta aaaggacac gatgatcat gtgatcacg 
      tgcttgcag ttctgtggg aatcgcatt gtgagcgga 
      tcggatgat aatacgctc aaagtttgg tcagcgatc 
      acaggaaag tgtttgcgc actttggtg gggcacact 
      ggaggcgtt tggtcgtct cagatgtcc ggtgcgaca 
      ataatcagt ggcagtact gatagaaca ctgaaggtg 
      tgggacgcc gaaacgggc gattgtatt catacgctc 
      aacggacac acttcaact gtacggtgt atgcattta 
      cacggaaac aaggttgtg agtggttct cgtgatgca 
      actttaagg gtgtgggat attaaaact ggggcatgt 
      cttcatgtt cttgtggga catttagcc gctgtacga 
      tgtgttcaa tatgatggc cgattggtg gtttctgga 
      gcttatgat tatatggtt aaagtttgg aatccagaa 
      cgagaggag tgtttgcac acgttacaa ggtcataca 
      aatcgtgtc tattcatta cagtttgat ggcgttcat 
      gtggtcagc ggttctttg gatacgagc attcgagtg 
      tgggaagtt gagacggga gcttgcaaa catacgctt 
      atgggtcac cagtcccta acatccggt atggaatta 
      cgaaataat attttagtg tccggaaat gccgattcc 
      accgttaaa gtttgggac atagtgacc ggtcagtgc 
      ctacaaacc ctttcagga ccatacaaa catcagtca 
      gccgtaact tgcctccaa ttcaacaat cgttttgtg 
      ataacttcc tcggatgat ggaactgta aaattatgg 
      gacgttcgc accggcgag ttcatcaga aacctggtg 
      gctttggac agtggtggc tcaggaggc gttgtgtgg 
      cggattcgc gccaacgac acgaaacta gtgtgcgcg 
      gttggaagt cgtaacggc acagaagaa acaaaacta 
      ctagtgttg gactttgat gtcgattct aactccagt 
      accatcatt tctaggtaa
    • >TC006451 RB
      atggcagaa tcatgtcct tcaggcaga agtaatttc 
      aacccctta tctagtatt aatcgagat tcagaactt 
      ccaaaacaa gactgcacc agccctagc cctactgaa 
      accgtttgt ctagagtcg ttgtgtacc actgataat 
      gcaccaggt acatcccag aatcactta gctccattg 
      agtgaaaaa ctagatgag atatttaga attagtcaa 
      tcgggcgag gagactgaa aaaactagc tttctcaat 
      aagctaggt ctggaagaa tgtgatgaa gaagacagt 
      tttgagaag gaagaaaac tgggaggat tgtactgaa 
      ggagacctc tctgaagaa aatcaagag ctagaagag 
      gacgaagat agcagtgaa gttttttca ggttcacct 
      ccaggtcct catcaaaca acaccaagg agttcgtac 
      gcgaagaga agtgggtcg tacgccact gacagatac 
      tggagaaaa cggaagttg gacgtgcag aatgggttt 
      ccgtgcaag aagttgcta ccagaaaag aaatctgac 
      gttacttcg atgtcatct gagccctct gctcgtgcg 
      ccgacggcg cccgaaacc aacggtgcc acccctagc 
      gacaaaaaa tctcgtctc gtaatcccg accaaagac 
      aatccgcca ccggaaatg tccgattgg ctgacccaa 
      ttccaaaaa tggtcaaac gcggaacga attctcgca 
      atcagcgaa ctaatcgcc cgctgcgaa ccaactcaa 
      gtccgtcat atgatgcaa gtgattgaa ccacaattc 
      caacgagat ttcatctca ttattacca cgcgagttg 
      gctttgaac gttttatca tttctcgaa ccgaaagac 
      cttttacgt gcagcacaa acttgtcgc agttggcgt 
      ttcctggct gaagataat ctactgtgg aaggaaaag 
      tgtcggcaa gcaggcatt gatgaaata cctaaacgg 
      aggtgctca cctaggtct cctggaacg caaatgtcg 
      ccatggaaa gctgcctac atgagacaa catgccatt 
      gaaatgaac tggaggagc aaaccgatc aggccgcct 
      aagatgcta aaaggacac gatgatcat gtgatcacg 
      tgcttgcag ttctgtggg aatcgcatt gtgagcgga 
      tcggatgat aatacgctc aaagtttgg tcagcgatc 
      acaggaaag tgtttgcgc actttggtg gggcacact 
      ggaggcgtt tggtcgtct cagatgtcc ggtgcgaca 
      ataatcagt ggcagtact gatagaaca ctgaaggtg 
      tgggacgcc gaaacgggc gattgtatt catacgctc 
      aacggacac acttcaact gtacggtgt atgcattta 
      cacggaaac aaggttgtg agtggttct cgtgatgca 
      actttaagg gtgtgggat attaaaact ggggcatgt 
      cttcatgtt cttgtggga catttagcc gctgtacga 
      tgtgttcaa tatgatggc cgattggtg gtttctgga 
      gcttatgat tatatggtt aaagtttgg aatccagaa 
      cgagaggag tgtttgcac acgttacaa ggtcataca 
      aatcgtgtc tattcatta cagtttgat ggcgttcat 
      gtggtcagc ggttctttg gatacgagc attcgagtg 
      tgggaagtt gagacggga gcttgcaaa catacgctt 
      atgggtcac cagtcccta acatccggt atggaatta 
      cgaaataat attttagtg tccggaaat gccgattcc 
      accgttaaa gtttgggac atagtgacc ggtcagtgc 
      ctacaaacc ctttcagga ccatacaaa catcagtca 
      gccgtaact tgcctccaa ttcaacaat cgttttgtg 
      ataacttcc tcggatgat ggaactgta aaattatgg 
      gacgttcgc accggcgag ttcatcaga aacctggtg 
      gctttggac agtggtggc tcaggaggc gttgtgtgg 
      cggattcgc gccaacgac acgaaacta gtgtgcgcg 
      gttggaagt cgtaacggc acagaagaa acaaaacta 
      ctagtgttg gactttgat gtcgattct aactccagt 
      accatcatt tctagctga
    mRNA sequence
    • >TC006451 RA
      aagttcgtc cagatattt gtaatggtg ccaataaat 
      cctagtatt ttgagaatg gccgtcagt gtagttttt 
      tgaaaaggt attttaccc agcgagatg cacaagaaa 
      actcgcttt agtgtctcg gagtgatag gaagtagca 
      aacagtggt ccttgatgc atagttttt ggaagcatt 
      tcgttaatt ttccttctt ctcttgcaa ttatctggt 
      agttagagt agtttcgtt tcatgcctt cgaataatg 
      atagaaaat tacatcagt agactgtag ataatggca 
      gaatcatgt ccttcaggc agaagtaat ttcaacccc 
      ttatctagt attaatcga gattcagaa cttccaaaa 
      caagactgc accagccct agccctact gaaaccgtt 
      tgtctagag tcgttgtgt accactgat aatgcacca 
      ggtacatcc cagaatcac ttagctcca ttgagtgaa 
      aaactagat gagatattt agaattagt caatcgggc 
      gaggagact gaaaaaact agctttctc aataagcta 
      ggtctggaa gaatgtgat gaagaagac agttttgag 
      aaggaagaa aactgggag gattgtact gaaggagac 
      ctctctgaa gaaaatcaa gagctagaa gaggacgaa 
      gatagcagt gaagttttt tcaggttca cctccaggt 
      cctcatcaa acaacacca aggagttcg tacgcgaag 
      agaagtggg tcgtacgcc actgacaga tactggaga 
      aaacggaag ttggacgtg cagaatggg tttccgtgc 
      aagaagttg ctaccagaa aagaaatct gacgttact 
      tcgatgtca tctgagccc tctgctcgt gcgccgacg 
      gcgcccgaa accaacggt gccacccct agcgacaaa 
      aaatctcgt ctcgtaatc ccgaccaaa gacaatccg 
      ccaccggaa atgtccgat tggctgacc caattccaa 
      aaatggtca aacgcggaa cgaattctc gcaatcagc 
      gaactaatc gcccgctgc gaaccaact caagtccgt 
      catatgatg caagtgatt gaaccacaa ttccaacga 
      gatttcatc tcattatta ccacgcgag ttggctttg 
      aacgtttta tcatttctc gaaccgaaa gacctttta 
      cgtgcagca caaacttgt cgcagttgg cgtttcctg 
      gctgaagat aatctactg tggaaggaa aagtgtcgg 
      caagcaggc attgatgaa atacctaaa cggaggtgc 
      tcacctagg tctcctgga acgcaaatg tcgccatgg 
      aaagctgcc tacatgaga caacatgcc attgaaatg 
      aactggagg agcaaaccg atcaggccg cctaagatg 
      ctaaaagga cacgatgat catgtgatc acgtgcttg 
      cagttctgt gggaatcgc attgtgagc ggatcggat 
      gataatacg ctcaaagtt tggtcagcg atcacagga 
      aagtgtttg cgcactttg gtggggcac actggaggc 
      gtttggtcg tctcagatg tccggtgcg acaataatc 
      agtggcagt actgataga acactgaag gtgtgggac 
      gccgaaacg ggcgattgt attcatacg ctcaacgga 
      cacacttca actgtacgg tgtatgcat ttacacgga 
      aacaaggtt gtgagtggt tctcgtgat gcaacttta 
      agggtgtgg gatattaaa actggggca tgtcttcat 
      gttcttgtg ggacattta gccgctgta cgatgtgtt 
      caatatgat ggccgattg gtggtttct ggagcttat 
      gattatatg gttaaagtt tggaatcca gaacgagag 
      gagtgtttg cacacgtta caaggtcat acaaatcgt 
      gtctattca ttacagttt gatggcgtt catgtggtc 
      agcggttct ttggatacg agcattcga gtgtgggaa 
      gttgagacg ggagcttgc aaacatacg cttatgggt 
      caccagtcc ctaacatcc ggtatggaa ttacgaaat 
      aatatttta gtgtccgga aatgccgat tccaccgtt 
      aaagtttgg gacatagtg accggtcag tgcctacaa 
      accctttca ggaccatac aaacatcag tcagccgta 
      acttgcctc caattcaac aatcgtttt gtgataact 
      tcctcggat gatggaact gtaaaatta tgggacgtt 
      cgcaccggc gagttcatc agaaacctg gtggctttg 
      gacagtggt ggctcagga ggcgttgtg tggcggatt 
      cgcgccaac gacacgaaa ctagtgtgc gcggttgga 
      agtcgtaac ggcacagaa gaaacaaaa ctactagtg 
      ttggacttt gatgtcgat tctaactcc agtaccatc 
      atttctagg taattacag actgcaaaa aattgtatg 
      agtgcgcgt gtttgtaga cctacgact tttaaaaag 
      catactact gatgttgct atcaaattt catgtcttt 
      gacaaaccc aataatttc aactactaa acgttttca 
      gaaacactt aatggtgta attctgtga tattacgtt 
      taatttagg cgacatcaa cagtatgcg aattttaac 
      gacttgtta tttcagctg aggagtttt tttcaaaaa 
      tctggtgct gttgagtcc ggaagtttc ttttgtgtt 
      gtgtccaaa gtacaaata ctgttttag gatgatgag 
      tttgcaata tttccacac tcgttgttt gtttaattg 
      taaaattcc taaatttac atgacatga tttgcacca 
      gagagactg attgtaagt tttaagtta atggcattt 
      taagtaaaa ttaccacat tagtttgtt tacaacgag 
      taataggcc aagtgattg gtaattgta tcacgtggt 
      ttattacga aaggagatt gttttttta gtgagagac 
      gatgtggtt catgctcaa gattcttgt catagctca 
      acataattg tatatagtc gaacataat ccgtttcat 
      catacatca ctagtttaa ttagtattt ttctcaatt 
      aatttactc aagttaagt atttttaat tagctcttg 
      tcaaaatgt ctaatagtt tagtcggca atcgaaatt 
      ttaacaatt ttaggaatc catgtttta tatacgaaa 
      atagtatca aataagaag agacgctta gattctttt 
      aagtcggat tttttcgtt tcgttcaga cttttcaaa 
      tttcctcgt ttttggttg gtgaatttt aatgtaatc 
      atttcaaag tctggtgtt gttaatgtt a
    • >TC006451 RB
      aagttcgtc cagatattt gtaatggtg ccaataaat 
      cctagtatt ttgagaatg gccgtcagt gtagttttt 
      tgaaaaggt attttaccc agcgagatg cacaagaaa 
      actcgcttt agtgtctcg gagtgatag gaagtagca 
      aacagtggt ccttgatgc atagttttt ggaagcatt 
      tcgttaatt ttccttctt ctcttgcaa ttatctggt 
      agttagagt agtttcgtt tcatgcctt cgaataatg 
      atagaaaat tacatcagt agactgtag ataatggca 
      gaatcatgt ccttcaggc agaagtaat ttcaacccc 
      ttatctagt attaatcga gattcagaa cttccaaaa 
      caagactgc accagccct agccctact gaaaccgtt 
      tgtctagag tcgttgtgt accactgat aatgcacca 
      ggtacatcc cagaatcac ttagctcca ttgagtgaa 
      aaactagat gagatattt agaattagt caatcgggc 
      gaggagact gaaaaaact agctttctc aataagcta 
      ggtctggaa gaatgtgat gaagaagac agttttgag 
      aaggaagaa aactgggag gattgtact gaaggagac 
      ctctctgaa gaaaatcaa gagctagaa gaggacgaa 
      gatagcagt gaagttttt tcaggttca cctccaggt 
      cctcatcaa acaacacca aggagttcg tacgcgaag 
      agaagtggg tcgtacgcc actgacaga tactggaga 
      aaacggaag ttggacgtg cagaatggg tttccgtgc 
      aagaagttg ctaccagaa aagaaatct gacgttact 
      tcgatgtca tctgagccc tctgctcgt gcgccgacg 
      gcgcccgaa accaacggt gccacccct agcgacaaa 
      aaatctcgt ctcgtaatc ccgaccaaa gacaatccg 
      ccaccggaa atgtccgat tggctgacc caattccaa 
      aaatggtca aacgcggaa cgaattctc gcaatcagc 
      gaactaatc gcccgctgc gaaccaact caagtccgt 
      catatgatg caagtgatt gaaccacaa ttccaacga 
      gatttcatc tcattatta ccacgcgag ttggctttg 
      aacgtttta tcatttctc gaaccgaaa gacctttta 
      cgtgcagca caaacttgt cgcagttgg cgtttcctg 
      gctgaagat aatctactg tggaaggaa aagtgtcgg 
      caagcaggc attgatgaa atacctaaa cggaggtgc 
      tcacctagg tctcctgga acgcaaatg tcgccatgg 
      aaagctgcc tacatgaga caacatgcc attgaaatg 
      aactggagg agcaaaccg atcaggccg cctaagatg 
      ctaaaagga cacgatgat catgtgatc acgtgcttg 
      cagttctgt gggaatcgc attgtgagc ggatcggat 
      gataatacg ctcaaagtt tggtcagcg atcacagga 
      aagtgtttg cgcactttg gtggggcac actggaggc 
      gtttggtcg tctcagatg tccggtgcg acaataatc 
      agtggcagt actgataga acactgaag gtgtgggac 
      gccgaaacg ggcgattgt attcatacg ctcaacgga 
      cacacttca actgtacgg tgtatgcat ttacacgga 
      aacaaggtt gtgagtggt tctcgtgat gcaacttta 
      agggtgtgg gatattaaa actggggca tgtcttcat 
      gttcttgtg ggacattta gccgctgta cgatgtgtt 
      caatatgat ggccgattg gtggtttct ggagcttat 
      gattatatg gttaaagtt tggaatcca gaacgagag 
      gagtgtttg cacacgtta caaggtcat acaaatcgt 
      gtctattca ttacagttt gatggcgtt catgtggtc 
      agcggttct ttggatacg agcattcga gtgtgggaa 
      gttgagacg ggagcttgc aaacatacg cttatgggt 
      caccagtcc ctaacatcc ggtatggaa ttacgaaat 
      aatatttta gtgtccgga aatgccgat tccaccgtt 
      aaagtttgg gacatagtg accggtcag tgcctacaa 
      accctttca ggaccatac aaacatcag tcagccgta 
      acttgcctc caattcaac aatcgtttt gtgataact 
      tcctcggat gatggaact gtaaaatta tgggacgtt 
      cgcaccggc gagttcatc agaaacctg gtggctttg 
      gacagtggt ggctcagga ggcgttgtg tggcggatt 
      cgcgccaac gacacgaaa ctagtgtgc gcggttgga 
      agtcgtaac ggcacagaa gaaacaaaa ctactagtg 
      ttggacttt gatgtcgat tctaactcc agtaccatc 
      atttctagc tgaggagtt tttttcaaa aatctggtg 
      ctgttgagt ccggaagtt tcttttgtg ttgtgtcca 
      aagtacaaa tactgtttt aggatgatg agtttgcaa 
      tatttccac actcgttgt ttgtttaat tgtaaaatt 
      cctaaattt acatgacat gatttgcac cagagagac 
      tgattgtaa gttttaagt taatggcat tttaagtaa 
      aattaccac attagtttg tttacaacg agtaatagg 
      ccaagtgat tggtaattg tatcacgtg gtttattac 
      gaaaggaga ttgtttttt tagtgagag acgatgtgg 
      ttcatgctc aagattctt gtcatagct caacataat 
      tgtatatag tcgaacata atccgtttc atcatacat 
      cactagttt aattagtat ttttctcaa ttaatttac 
      tcaagttaa gtattttta attagctct tgtcaaaat 
      gtctaatag tttagtcgg caatcgaaa ttttaacaa 
      ttttaggaa tccatgttt tatatacga aaatagtat 
      caaataaga agagacgct tagattctt ttaagtcgg 
      attttttcg tttcgttca gacttttca aatttcctc 
      gtttttggt tggtgaatt ttaatgtaa tcatttcaa 
      agtctggtg ttgttaatg tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY