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Gene information
General information
TC034091 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9800497..9802353
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034091
    iBeetle screen iB_07506

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034091 RA
      MSSILKFIK YAFTKYPIA SNSLVYGTL 
      YVGAEFSQQ TISRKVLTK TPTSYDTPA 
      IARYAVYGT GIQGPFLTI WYRWLDKKF 
      IGTSYKIVA QKLLLDQFL MTPQILVIF 
      YVAMSIMEK KKDIFEECK QKIIPTFKS 
      SCMFWLPAQ TVNFLLIPP SFRVVYVGT 
      CSFAWVNIL CWIKRQEY
    • >TC034091 RB
      MSSILKFIK YAFTKYPIA SNSLVYGTL 
      YVGAEFSQQ TISRKVLTK TPTSYDTPA 
      IARYAVYGT GIQGPFLTI WYRWLDKKF 
      IGTSYKIVA QKLLLDQFL MTPQILVIF 
      YVAMSIMEK KKDIFEECK QKIIPTFKS 
      SWSFMLGLA HLHGSTFCA GLSDKNTRF 
      CKL
    • >TC034091 RC
      MSSILKFIK YAFTKYPIA SNSLVYGTL 
      YVGAEFSQQ TISRKVLTK TPTSYDTPA 
      IARYAVYGT GIQGPFLTI WYLFFWNKE 
      PIIYDRQML TCFGILGVT VIAPVYQYW 
      YRWLDKKFI GTSYKIVAQ KLLLDQFLM 
      TPQILVIFY VAMSIMEKK KDIFEECKQ 
      KIIPTFKSS CMFWLPAQT VNFLLIPPS 
      FRVVYVGTC SFAWVNILC WIKRQEY
    CDS sequence
    • >TC034091 RA
      atgtcaagt attttaaaa ttcattaaa tatgcattt 
      accaaatac ccgatcgct tccaattct ctggtttat 
      ggaacactt tacgtggga gccgaattt tcccaacaa 
      accatctcg agaaaagtt ctgactaaa acgcccacg 
      tcttacgac acgcctgca attgctcga tatgcagtt 
      tacggaacc ggaatacag ggaccattt ttgaccatt 
      tggtacaga tggttagac aagaaattt atcggaacc 
      tcgtacaaa attgtagca caaaaactg ctactggac 
      caattctta atgaccccg cagattttg gtaattttt 
      tatgttgcc atgagcatt atggagaag aagaaggat 
      atttttgaa gaatgtaaa cagaaaata atccccaca 
      ttcaagtct agttgtatg ttttggctt ccagcccaa 
      actgtaaat ttcttgtta atcccccct tcgtttagg 
      gtcgtttat gttgggact tgctcattt gcatgggtc 
      aacattttg tgctggatt aagcgacaa gaatactag
    • >TC034091 RB
      atgtcaagt attttaaaa ttcattaaa tatgcattt 
      accaaatac ccgatcgct tccaattct ctggtttat 
      ggaacactt tacgtggga gccgaattt tcccaacaa 
      accatctcg agaaaagtt ctgactaaa acgcccacg 
      tcttacgac acgcctgca attgctcga tatgcagtt 
      tacggaacc ggaatacag ggaccattt ttgaccatt 
      tggtacaga tggttagac aagaaattt atcggaacc 
      tcgtacaaa attgtagca caaaaactg ctactggac 
      caattctta atgaccccg cagattttg gtaattttt 
      tatgttgcc atgagcatt atggagaag aagaaggat 
      atttttgaa gaatgtaaa cagaaaata atccccaca 
      ttcaagtct agttggtcg tttatgttg ggacttgct 
      catttgcat gggtcaaca ttttgtgct ggattaagc 
      gacaagaat actagattt tgtaaatta taa
    • >TC034091 RC
      atgtcaagt attttaaaa ttcattaaa tatgcattt 
      accaaatac ccgatcgct tccaattct ctggtttat 
      ggaacactt tacgtggga gccgaattt tcccaacaa 
      accatctcg agaaaagtt ctgactaaa acgcccacg 
      tcttacgac acgcctgca attgctcga tatgcagtt 
      tacggaacc ggaatacag ggaccattt ttgaccatt 
      tggtattta tttttttgg aataaagaa ccgatcatc 
      tatgaccgc caaatgctc acatgtttt ggaattctc 
      ggtgtgact gttattgct ccagtatac cagtactgg 
      tacagatgg ttagacaag aaatttatc ggaacctcg 
      tacaaaatt gtagcacaa aaactgcta ctggaccaa 
      ttcttaatg accccgcag attttggta attttttat 
      gttgccatg agcattatg gagaagaag aaggatatt 
      tttgaagaa tgtaaacag aaaataatc cccacattc 
      aagtctagt tgtatgttt tggcttcca gcccaaact 
      gtaaatttc ttgttaatc cccccttcg tttagggtc 
      gtttatgtt gggacttgc tcatttgca tgggtcaac 
      attttgtgc tggattaag cgacaagaa tactag
    mRNA sequence
    • >TC034091 RA
      acatatttt atttacagt aatttattt aactagcgc 
      caattgaat tcattaaac aaaacctat ctgctgtgg 
      tgtgtagta gctgtgaag aattacaag cgttgttat 
      taagttact gtgttgttc cagcatgtc aagtatttt 
      aaaattcat taaatatgc atttaccaa atacccgat 
      cgcttccaa ttctctggt ttatggaac actttacgt 
      gggagccga attttccca acaaaccat ctcgagaaa 
      agttctgac taaaacgcc cacgtctta cgacacgcc 
      tgcaattgc tcgatatgc agtttacgg aaccggaat 
      acagggacc atttttgac catttggta cagatggtt 
      agacaagaa atttatcgg aacctcgta caaaattgt 
      agcacaaaa actgctact ggaccaatt cttaatgac 
      cccgcagat tttggtaat tttttatgt tgccatgag 
      cattatgga gaagaagaa ggatatttt tgaagaatg 
      taaacagaa aataatccc cacattcaa gtctagttg 
      tatgttttg gcttccagc ccaaactgt aaatttctt 
      gttaatccc cccttcgtt tagggtcgt ttatgttgg 
      gacttgctc atttgcatg ggtcaacat tttgtgctg 
      gattaagcg acaagaata ctagatttt gtaaattat 
      aataaagtc aagtcttac attttgacg aagcttacg 
      aacttgtta aaatttgag ctttggctg ttcttaaat 
      cagtgacag aagaggaca ataattcct atttattgt 
      agctatgga tgttcttga tggcactat ttagctata 
      actttaaat aattatacc tccaatgtg atattttca 
      accgtacat atcaagtag tctcccaga aagtatgat 
      gttttattg actagaaat tatatatta actgaaccg 
      aatttattg atgttccat tttaatctc gtattggta 
      cagttgatg tgtttattt gtgtactac atgtaatat 
      tttgaatac ataataaat tttatctta ctcg
    • >TC034091 RB
      acatatttt atttacagt aatttattt aactagcgc 
      caattgaat tcattaaac aaaacctat ctgctgtgg 
      tgtgtagta gctgtgaag aattacaag cgttgttat 
      taagttact gtgttgttc cagcatgtc aagtatttt 
      aaaattcat taaatatgc atttaccaa atacccgat 
      cgcttccaa ttctctggt ttatggaac actttacgt 
      gggagccga attttccca acaaaccat ctcgagaaa 
      agttctgac taaaacgcc cacgtctta cgacacgcc 
      tgcaattgc tcgatatgc agtttacgg aaccggaat 
      acagggacc atttttgac catttggta cagatggtt 
      agacaagaa atttatcgg aacctcgta caaaattgt 
      agcacaaaa actgctact ggaccaatt cttaatgac 
      cccgcagat tttggtaat tttttatgt tgccatgag 
      cattatgga gaagaagaa ggatatttt tgaagaatg 
      taaacagaa aataatccc cacattcaa gtctagttg 
      gtcgtttat gttgggact tgctcattt gcatgggtc 
      aacattttg tgctggatt aagcgacaa gaatactag 
      attttgtaa attataata aagtcaagt cttacattt 
      tgacgaagc ttacgaact tgttaaaat ttgagcttt 
      ggctgttct taaatcagt gacagaaga ggacaataa 
      ttcctattt attgtagct atggatgtt cttgatggc 
      actatttag ctataactt taaataatt atacctcca 
      atgtgatat tttcaaccg tacatatca agtagtctc 
      ccagaaagt atgatgttt tattgacta gaaattata 
      tattaactg aaccgaatt tattgatgt tccatttta 
      atctcgtat tggtacagt tgatgtgtt tatttgtgt 
      actacatgt aatattttg aatacataa taaatttta 
      tcttactcg
    • >TC034091 RC
      acatatttt atttacagt aatttattt aactagcgc 
      caattgaat tcattaaac aaaacctat ctgctgtgg 
      tgtgtagta gctgtgaag aattacaag cgttgttat 
      taagttact gtgttgttc cagcatgtc aagtatttt 
      aaaattcat taaatatgc atttaccaa atacccgat 
      cgcttccaa ttctctggt ttatggaac actttacgt 
      gggagccga attttccca acaaaccat ctcgagaaa 
      agttctgac taaaacgcc cacgtctta cgacacgcc 
      tgcaattgc tcgatatgc agtttacgg aaccggaat 
      acagggacc atttttgac catttggta tttattttt 
      ttggaataa agaaccgat catctatga ccgccaaat 
      gctcacatg ttttggaat tctcggtgt gactgttat 
      tgctccagt ataccagta ctggtacag atggttaga 
      caagaaatt tatcggaac ctcgtacaa aattgtagc 
      acaaaaact gctactgga ccaattctt aatgacccc 
      gcagatttt ggtaatttt ttatgttgc catgagcat 
      tatggagaa gaagaagga tatttttga agaatgtaa 
      acagaaaat aatccccac attcaagtc tagttgtat 
      gttttggct tccagccca aactgtaaa tttcttgtt 
      aatcccccc ttcgtttag ggtcgttta tgttgggac 
      ttgctcatt tgcatgggt caacatttt gtgctggat 
      taagcgaca agaatacta gattttgta aattataat 
      aaagtcaag tcttacatt ttgacgaag cttacgaac 
      ttgttaaaa tttgagctt tggctgttc ttaaatcag 
      tgacagaag aggacaata attcctatt tattgtagc 
      tatggatgt tcttgatgg cactattta gctataact 
      ttaaataat tatacctcc aatgtgata ttttcaacc 
      gtacatatc aagtagtct cccagaaag tatgatgtt 
      ttattgact agaaattat atattaact gaaccgaat 
      ttattgatg ttccatttt aatctcgta ttggtacag 
      ttgatgtgt ttatttgtg tactacatg taatatttt 
      gaatacata ataaatttt atcttactc g
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