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Gene information
General information
TC004767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6659777..6662238
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004767
    iBeetle screen iB_07501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature random rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature specific subset not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004767 RA
      MASTFDHED KDSWYFGPI SRKEATDLL MAERGGGVF 
      LVRDSATCV GDFVLCVKE DSKVSHYII NKMQESQQI 
      KYRIGDQIF ADLPSLLSF YKLHYLDTT PLIRPASKK 
      VEKVIAKFD FEGQDPDDL VFRKGEILT IVAKDEEQW 
      WTAVNSAGQ RGSIPVPYV QKYEEGQAP TEIPRPGSG 
      GSVTSSTQQ QAETAMKRS QMQRKLPAK ARVKQVRVP 
      NAYDKTALK LEIGDIITV TKTNINGQW EGELNGKTG 
      YFPFTHVEF LDNDNEI
    CDS sequence
    • >TC004767 RA
      atggctagt acatttgat catgaagat aaggacagc 
      tggtatttt ggtccaatt agcaggaaa gaagctacc 
      gatttgctc atggcagaa cggggaggt ggtgttttc 
      ttagtgcgt gatagtgct acttgtgtt ggagacttt 
      gtattgtgt gtaaaggaa gacagtaaa gtgagccat 
      tacataatt aataaaatg caagaatcg caacaaatt 
      aagtatagg attggagat caaattttt gcagattta 
      ccaagcttg ctttcattc tataagctt cattattta 
      gatacaaca ccacttatt agacctgct tctaaaaaa 
      gtagagaaa gttattgct aaatttgat tttgagggt 
      caggatcct gatgatcta gtctttaga aaaggagag 
      atccttaca attgtcgca aaagatgaa gagcagtgg 
      tggactgcc gttaatagt gctggacaa agaggatct 
      atacctgtt ccatatgta caaaagtat gaagaagga 
      caagctcct actgaaatt ccgaggcct ggttcaggg 
      ggtagtgta acaagttcg acacaacaa caagccgaa 
      actgccatg aaacgaagt cagatgcaa cgaaaactt 
      ccagcgaaa gcacgagtg aaacaagta cgtgtccca 
      aacgcttac gataaaacc gctttaaaa ctggaaatc 
      ggggatatt atcactgtt actaagact aacataaat 
      ggacagtgg gaaggggag ttaaatgga aaaactggc 
      tattttcct tttacacat gtagaattt ttagataat 
      gataatgaa atttag
    mRNA sequence
    • >TC004767 RA
      aggaacaaa tatttgtag ataagttaa ataagggct 
      tggtagtgg cttggttgt cattatcat tatcattgt 
      caatttcga accgatatg tcattttga caaaagacg 
      catctgtca gtagtgtca atggaaaag tctggtata 
      aatcggtat cggaaataa ggccatttg tatgcgttt 
      gtgtttgaa atccaaatt tttagaaag ggaatttct 
      cgttagttt ttatatttt gtggcctaa ggcttggag 
      gcaggccag gtcagtcgg ccatatctg gcgctgctg 
      gtgttggct gctccgaga tcgactgta taccacgga 
      ccacggtag tatcatgcg ataaatttt aacaattgt 
      gtggtgcag ccgctgata agagtgtag ggacatgtc 
      tgtttaata aacaggggt gaaggtgca agtttgggg 
      taatttaac cggaatttg aggccattt aaaaactgc 
      agcaatatg tgtcgcttg aagaagact cataaatgc 
      ataatataa tatgagaaa aaaaattag gttaaaata 
      cgccaatta ataatagcg ttaaacaat attggactg 
      tacataggt atgtaaatg tctttattt attacaagt 
      tggagtacc ttcagaaaa tgcttaacc gttgctatt 
      atattatca agtggtatt aaaaaatag tgcaaacca 
      tagaaaatg gctagtaca tttgatcat gaagataag 
      gacagctgg tattttggt ccaattagc aggaaagaa 
      gctaccgat ttgctcatg gcagaacgg ggaggtggt 
      gttttctta gtgcgtgat agtgctact tgtgttgga 
      gactttgta ttgtgtgta aaggaagac agtaaagtg 
      agccattac ataattaat aaaatgcaa gaatcgcaa 
      caaattaag tataggatt ggagatcaa atttttgca 
      gatttacca agcttgctt tcattctat aagcttcat 
      tatttagat acaacacca cttattaga cctgcttct 
      aaaaaagta gagaaagtt attgctaaa tttgatttt 
      gagggtcag gatcctgat gatctagtc tttagaaaa 
      ggagagatc cttacaatt gtcgcaaaa gatgaagag 
      cagtggtgg actgccgtt aatagtgct ggacaaaga 
      ggatctata cctgttcca tatgtacaa aagtatgaa 
      gaaggacaa gctcctact gaaattccg aggcctggt 
      tcagggggt agtgtaaca agttcgaca caacaacaa 
      gccgaaact gccatgaaa cgaagtcag atgcaacga 
      aaacttcca gcgaaagca cgagtgaaa caagtacgt 
      gtcccaaac gcttacgat aaaaccgct ttaaaactg 
      gaaatcggg gatattatc actgttact aagactaac 
      ataaatgga cagtgggaa ggggagtta aatggaaaa 
      actggctat tttcctttt acacatgta gaattttta 
      gataatgat aatgaaatt tagtgtttt ttattgatt 
      atattattg tataaatgc tccattctg ctgtatatt 
      tattaaatt tatttggca ctaacatac aatgtttat 
      cctgattac aagctattt gcgttgtta atatcgaat 
      tgtattttt attaattac atatcaata tttacataa 
      atggaattt attaaccgt gctttaatt gtacaacaa 
      aaggaacca accctgcat cggaatatt taaatctcc 
      cgctcactg aaagagccc gcctttatg caacatgtt 
      agttccctc ccgctttca aggtcattg ctctaaaac 
      ccatacata aaaacagtt gtacctgat taccttatg 
      ttctgtacg aaatattga ataaaacat cattatgaa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY