Status
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Gene information
General information
TC031279
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005691,TC005690
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9074333..9139213
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031279
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    gena triplet bristle potentially number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    vertex triplet potentially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    bell row potentially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031279 RA
      MMSVSSLCH ISTIFFITA SINFGTGVK INKLQVPEV 
      IKHGAPVIL DCDFTLDDT EEDLVVKWY FNKNKTLVY 
      QWIPALKPQ GLGILKDRL NLEYAASVD ANSVHRALH 
      ILKAVPDLS GDYTCSVST LQSEDIRTK SMLVFVPEK 
      ELLLRRLEA EEGLMRVQC LADGVFPRP VMSLHSQER 
      EIEGTEVTA RLRGQLYEV SATATLPAL KDPEEFSCE 
      LRIPQANYT VRRETVFYP GNKAPLILM DRWLALSPC 
      IFSLYLGLT RQFIGLC
    • >TC031279 RB
      MMSVSSLCH ISTIFFITA SINFGTGVK INKLQVPEV 
      IKHGAPVIL DCDFTLDDT EEDLVVKWY FNKNKTLVY 
      QWIPALKPQ GLGILKDRL NLEYAASVD ANSVHRALH 
      ILKAVPDLS GDYTCSVST LQSEDIRTK SMLVFVPEK 
      ELLLRRLEA EEGLMRVQC LADGVFPRP VMSLHSQER 
      EIEGTEVTA RLRGQLYEV SATATLPAL KDPEEFSCE 
      LRIPQANYT VRRETVFYP VIQINP
    CDS sequence
    • >TC031279 RA
      atgatgagt gtttccagt ctgtgccat atttccacc 
      atatttttc attactgct tcgattaat tttggaaca 
      ggggtgaaa atcaacaaa ttgcaagtt cctgaagta 
      atcaagcac ggggcgcct gtaattcta gactgcgac 
      tttacgtta gatgacaca gaggaggac ctcgtcgtg 
      aaatggtat ttcaacaag aataaaaca ctagtctac 
      cagtggata ccagcgttg aagccgcaa ggtttgggc 
      attttgaaa gatcgcctc aatcttgaa tatgccgcc 
      agcgtcgac gcaaatagt gtccacaga gccctgcat 
      atccttaag gccgttcct gacctctcc ggggactat 
      acgtgctca gtatcaacc ctccagagc gaagacatc 
      aggactaaa agcatgctc gtatttgtt cccgaaaaa 
      gagcttctt ctgaggaga ctggaggct gaagaagga 
      ctcatgcgg gtgcaatgt ctggcagac ggcgtattt 
      cccaggcca gttatgagt ctgcactct caagaaagg 
      gagatcgaa gggaccgaa gtcacagct cggctaagg 
      ggacagctg tacgaagta tcggcgaca gcgacgctg 
      cctgcgctt aaggaccca gaggagttc tcgtgcgag 
      cttcgcatc ccccaggcg aactataca gttcgtcga 
      gagaccgtc ttctatccc ggcaacaaa gcaccttta 
      attttaatg gatagatgg ctggccctc tccccttgc 
      atattttcc ctatacctt ggcctaaca cgtcagttt 
      ataggtctg tgctaa
    • >TC031279 RB
      atgatgagt gtttccagt ctgtgccat atttccacc 
      atatttttc attactgct tcgattaat tttggaaca 
      ggggtgaaa atcaacaaa ttgcaagtt cctgaagta 
      atcaagcac ggggcgcct gtaattcta gactgcgac 
      tttacgtta gatgacaca gaggaggac ctcgtcgtg 
      aaatggtat ttcaacaag aataaaaca ctagtctac 
      cagtggata ccagcgttg aagccgcaa ggtttgggc 
      attttgaaa gatcgcctc aatcttgaa tatgccgcc 
      agcgtcgac gcaaatagt gtccacaga gccctgcat 
      atccttaag gccgttcct gacctctcc ggggactat 
      acgtgctca gtatcaacc ctccagagc gaagacatc 
      aggactaaa agcatgctc gtatttgtt cccgaaaaa 
      gagcttctt ctgaggaga ctggaggct gaagaagga 
      ctcatgcgg gtgcaatgt ctggcagac ggcgtattt 
      cccaggcca gttatgagt ctgcactct caagaaagg 
      gagatcgaa gggaccgaa gtcacagct cggctaagg 
      ggacagctg tacgaagta tcggcgaca gcgacgctg 
      cctgcgctt aaggaccca gaggagttc tcgtgcgag 
      cttcgcatc ccccaggcg aactataca gttcgtcga 
      gagaccgtc ttctatccc gtaattcaa atcaatcct 
      taa
    mRNA sequence
    • >TC031279 RA
      agtttgcag cgtcgggac gccgacgtc ggaacatcg 
      cgtcatcac tcatcacac gggagaaca cgtgcgttc 
      ggtggcgtg ctctaattt caaatcaat cagacctgt 
      ctattcctc gcagaatgg tttgcatgt aaggaaagt 
      cgagtgttg ataattaaa gaggaatgt gagtgtccg 
      taaatgatg agtgtttcc agtctgtgc catatttcc 
      accatattt ttcattact gcttcgatt aattttgga 
      acaggggtg aaaatcaac aaattgcaa gttcctgaa 
      gtaatcaag cacggggcg cctgtaatt ctagactgc 
      gactttacg ttagatgac acagaggag gacctcgtc 
      gtgaaatgg tatttcaac aagaataaa acactagtc 
      taccagtgg ataccagcg ttgaagccg caaggtttg 
      ggcattttg aaagatcgc ctcaatctt gaatatgcc 
      gccagcgtc gacgcaaat agtgtccac agagccctg 
      catatcctt aaggccgtt cctgacctc tccggggac 
      tatacgtgc tcagtatca accctccag agcgaagac 
      atcaggact aaaagcatg ctcgtattt gttcccgaa 
      aaagagctt cttctgagg agactggag gctgaagaa 
      ggactcatg cgggtgcaa tgtctggca gacggcgta 
      tttcccagg ccagttatg agtctgcac tctcaagaa 
      agggagatc gaagggacc gaagtcaca gctcggcta 
      aggggacag ctgtacgaa gtatcggcg acagcgacg 
      ctgcctgcg cttaaggac ccagaggag ttctcgtgc 
      gagcttcgc atcccccag gcgaactat acagttcgt 
      cgagagacc gtcttctat cccggcaac aaagcacct 
      ttaatttta atggataga tggctggcc ctctcccct 
      tgcatattt tccctatac cttggccta acacgtcag 
      tttataggt ctgtgctaa ctataggta atggaagac 
      aagacaatg attctaacg taattttta gtgtgcaac 
      tgaagactc acgctaaaa actgaatgt tacgatgtt 
      atttgtatt aatttcttt gaagactgt tttctgtaa 
      cacctccat atacgcaac agttaccag cactaaacc 
      ggaacaaac tagacattt caactgtaa ataaattgt 
      cgtaaagga aagacattg cttttagta ttccttttt 
      gtaaagcat gctttgtta atccggcga ttgacaaac 
      catgttggg ataaataag tgtattcaa agttagtgt 
      ctaagataa taactattt gtgataaaa tttaattct 
      attcatgtt acgataaaa ctatttcct gattgttta 
      ttatatgtt ggccgttgt acatattac gtagcttaa 
      aactgaata aaatctcaa tttgtac
    • >TC031279 RB
      agtttgcag cgtcgggac gccgacgtc ggaacatcg 
      cgtcatcac tcatcacac gggagaaca cgtgcgttc 
      ggtggcgtg ctctaattt caaatcaat cagacctgt 
      ctattcctc gcagaatgg tttgcatgt aaggaaagt 
      cgagtgttg ataattaaa gaggaatgt gagtgtccg 
      taaatgatg agtgtttcc agtctgtgc catatttcc 
      accatattt ttcattact gcttcgatt aattttgga 
      acaggggtg aaaatcaac aaattgcaa gttcctgaa 
      gtaatcaag cacggggcg cctgtaatt ctagactgc 
      gactttacg ttagatgac acagaggag gacctcgtc 
      gtgaaatgg tatttcaac aagaataaa acactagtc 
      taccagtgg ataccagcg ttgaagccg caaggtttg 
      ggcattttg aaagatcgc ctcaatctt gaatatgcc 
      gccagcgtc gacgcaaat agtgtccac agagccctg 
      catatcctt aaggccgtt cctgacctc tccggggac 
      tatacgtgc tcagtatca accctccag agcgaagac 
      atcaggact aaaagcatg ctcgtattt gttcccgaa 
      aaagagctt cttctgagg agactggag gctgaagaa 
      ggactcatg cgggtgcaa tgtctggca gacggcgta 
      tttcccagg ccagttatg agtctgcac tctcaagaa 
      agggagatc gaagggacc gaagtcaca gctcggcta 
      aggggacag ctgtacgaa gtatcggcg acagcgacg 
      ctgcctgcg cttaaggac ccagaggag ttctcgtgc 
      gagcttcgc atcccccag gcgaactat acagttcgt 
      cgagagacc gtcttctat cccgtaatt caaatcaat 
      ccttaataa ccctgttac accttgttt aacacttac 
      gtaataaaa atgttttca gaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY