Status
Please wait ...
Gene information
General information
TC005717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8537496..8540438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005717
    iBeetle screen iB_07495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005717 RA
      MPNKALHCY LLMIIWILL LVAGVYKFL EPRQRPPEP 
      AVTAELFHN LANDIKTRR IFKICNFPH EITDGDEGA 
      LHSKKWVMT GVIILIRHG DRGPLQHVR NISAVNCGS 
      QETEKLISY KSYLHNVTI SGRAPWTGP GPFHDFPLL 
      PTHPRQCQL GQLTMQGIS QLLKLGQIL RQTYLHVWP 
      KIATLTPSE VLVYSTRYR RTFQSALSF LFALIPTEA 
      FTKLSIFES QSMNFCFKD CGCPITDKL WKGVKKSIS 
      HQLKSHPAV AVLAEATGK SLFSTGAEQ GVLGGDPHA 
      VRDALLTYV CHGSGLPCE TPTNCIKRQ NVAGVFAYT 
      DWVNYQKWR NAFWKRLCL LRSYGLIRH IVQQMLHMV 
      SNNGPFLVL YSGHDHTVE QLTTALGLQ NDPLLLRYG 
      ARVVFEVYQ NYEESQNGA KGIYFRVLS NGRDVTRQV 
      SFCKDIVLL ENKVSLCKI EDIVRFIHD DYFVSLNVT 
      NFKDACSRN E
    CDS sequence
    • >TC005717 RA
      atgcccaac aaggccctc cactgctac ctcctgatg 
      atcatctgg atcctgctc ttggtcgca ggtgtttac 
      aaattcctc gaaccgcga cagcggccc ccagagccg 
      gcggtcacc gccgaactc ttccacaac ctcgccaac 
      gacatcaaa acacggcga attttcaaa atttgtaac 
      ttcccgcac gagatcacc gacggggat gaaggtgcc 
      ctccacagc aaaaagtgg gtgatgacg ggtgtcatc 
      attttgatc aggcatggg gacaggggg cccctccaa 
      cacgtcagg aatatctcg gcggtcaat tgcggctcg 
      caagaaaca gaaaaattg atttcttac aagtcgtat 
      ctccacaac gtgacaatc tcgggccgc gccccctgg 
      actggcccc ggccccttc cacgacttc cctctgctc 
      ccaacccac ccccggcag tgccaactg ggccaactc 
      accatgcaa ggcatcagc caactcctc aaacttggg 
      caaatatta cgccaaacc taccttcac gtttggcca 
      aaaatcgcc actctaaca cccagcgaa gtcctagtc 
      tacagcacc cgctatcgg cgcaccttc caatcagcc 
      ctctcgttc ttattcgct ttaatccct acagaagcg 
      ttcacaaaa ttatcaatt tttgaaagc caatcaatg 
      aatttctgc ttcaaagac tgcgggtgc cccatcacc 
      gacaagtta tggaaaggg gtgaaaaaa tcgatttcg 
      caccagttg aaatcgcac ccggccgtg gccgtcctg 
      gccgaagcc acggggaag tccctgttt tccaccggg 
      gcggagcag ggggtcctc ggcggggac ccccacgcc 
      gtcagagac gcactcttg acttacgtt tgccacggc 
      agtgggctc ccgtgtgaa accccgacg aattgtatc 
      aagcggcag aacgttgct ggtgttttt gcttacact 
      gactgggtc aactaccag aaatggagg aacgctttt 
      tggaaaaga ttgtgtctt ttgcggtct tacggtttg 
      ataagacac atcgtgcaa caaatgttg cacatggtg 
      tccaataac ggccccttt ctggtgctt tactcgggg 
      catgaccac actgtggag cagctcacc acggcccta 
      gggctccag aacgacccc ctgttgttg cgatatggg 
      gcgcgggtc gttttcgaa gtgtaccaa aactacgaa 
      gagtcgcaa aacggcgcc aaaggcatt tatttccgc 
      gtgttatcg aatggtaga gacgtgaca cgacaggtg 
      agtttttgt aaagatatc gttttgttg gaaaataaa 
      gtaagtttg tgtaaaatc gaggatatt gtgagattt 
      atccacgat gattatttc gtttcgttg aacgtaacg 
      aattttaaa gatgcgtgc agtaggaat gagtag
    mRNA sequence
    • >TC005717 RA
      aagcgataa gttaggtta tgtcagagg cggcttgat 
      gactttcga tgcacccag gatgcccaa caaggccct 
      ccactgcta cctcctgat gatcatctg gatcctgct 
      cttggtcgc aggtgttta caaattcct cgaaccgcg 
      acagcggcc cccagagcc ggcggtcac cgccgaact 
      cttccacaa cctcgccaa cgacatcaa aacacggcg 
      aattttcaa aatttgtaa cttcccgca cgagatcac 
      cgacgggga tgaaggtgc cctccacag caaaaagtg 
      ggtgatgac gggtgtcat cattttgat caggcatgg 
      ggacagggg gcccctcca acacgtcag gaatatctc 
      ggcggtcaa ttgcggctc gcaagaaac agaaaaatt 
      gatttctta caagtcgta tctccacaa cgtgacaat 
      ctcgggccg cgccccctg gactggccc cggcccctt 
      ccacgactt ccctctgct cccaaccca cccccggca 
      gtgccaact gggccaact caccatgca aggcatcag 
      ccaactcct caaacttgg gcaaatatt acgccaaac 
      ctaccttca cgtttggcc aaaaatcgc cactctaac 
      acccagcga agtcctagt ctacagcac ccgctatcg 
      gcgcacctt ccaatcagc cctctcgtt cttattcgc 
      tttaatccc tacagaagc gttcacaaa attatcaat 
      ttttgaaag ccaatcaat gaatttctg cttcaaaga 
      ctgcgggtg ccccatcac cgacaagtt atggaaagg 
      ggtgaaaaa atcgatttc gcaccagtt gaaatcgca 
      cccggccgt ggccgtcct ggccgaagc cacggggaa 
      gtccctgtt ttccaccgg ggcggagca gggggtcct 
      cggcgggga cccccacgc cgtcagaga cgcactctt 
      gacttacgt ttgccacgg cagtgggct cccgtgtga 
      aaccccgac gaattgtat caagcggca gaacgttgc 
      tggtgtttt tgcttacac tgactgggt caactacca 
      gaaatggag gaacgcttt ttggaaaag attgtgtct 
      tttgcggtc ttacggttt gataagaca catcgtgca 
      acaaatgtt gcacatggt gtccaataa cggcccctt 
      tctggtgct ttactcggg gcatgacca cactgtgga 
      gcagctcac cacggccct agggctcca gaacgaccc 
      cctgttgtt gcgatatgg ggcgcgggt cgttttcga 
      agtgtacca aaactacga agagtcgca aaacggcgc 
      caaaggcat ttatttccg cgtgttatc gaatggtag 
      agacgtgac acgacaggt gagtttttg taaagatat 
      cgttttgtt ggaaaataa agtaagttt gtgtaaaat 
      cgaggatat tgtgagatt tatccacga tgattattt 
      cgtttcgtt gaacgtaac gaattttaa agatgcgtg 
      cagtaggaa tgagtagaa atttgagtt gttaggttt 
      ctgaaactg tgattgtat cagacgtgt tttcgtttt 
      gagagcaga aatgtgata ggtcaatga cgcaaaaat 
      gaatggaat tgtttccag tcaaggcac aaaacggtt 
      gtgagcctg ttcaagaaa tgaattcac gctcttgaa 
      gggatttta tttcttctg tcttccgtg cttgcttat 
      tataggttg cgaaataac cgatatttt tattttgac 
      tcaatgaaa caaaagcac ttaattatg aaggatgat 
      tgcctggag ccctgatga ttattttta gtgttttcg 
      tacagatat gttatccac aagtgaaaa caataatta 
      attaatgtg atgtaacaa tgaacaaac tgtaatctc 
      ataaacccg ttatttatt ctaatctac acttttgta 
      ttgtgacgt tgggttgtt atttgtttt catcttttt 
      acttgaaaa ataaaagta gaaactttg ttgtttagt 
      atcattcac ggcttttca cccaaatgt caacgaatc 
      tgtaaaagc aaaggaaac gcaagtggt aattgcaaa 
      ggcaaaaaa aaagaattg tgttgactt ctattggct 
      taattgtgt ctgatttgt gtttacata aaaaagcga 
      ttattcaaa atttgtaat aaagtagct catttccga 
      aatcatttt atttcactt tttacatga aaattaaat 
      atttaattt ggaatttcg taatttttt gttaatcac 
      ccagcatat ttaaaaatt caaataaaa aaatcgtct 
      tcatttatt agaaagtgt gtagaaaat aaagctcaa 
      ttagatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY